Oxoid CM0233B Oxoid 胰蛋白示血琼脂基础 TRYPTOSE BLOOD AGAR BASE

商品名称：胰蛋白示血琼脂基础
品牌：Oxoid
货号：CM0233B
规格：500g

TRYPTOSE BLOOD AGAR BASE

Code: CM0233

A highly nutritious medium specially developed for the preparation of a blood agar which will support the growth of fastidious organisms.

一种高营养培养基，专为制备血琼脂而开发，该培养基将支持苛求微生物的生长。

* Adjusted as required to meet performance standards

Directions
Suspend 30g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

For blood agar, cool the basal medium to 45-50°C and add 7% of sterile blood. Mix thoroughly, taking care to avoid incorporation of air bubbles, and dispense into Petri dishes or other containers.

Description
A highly nutritious medium specially developed by Casman^1,2 for the preparation of a blood agar which will support the growth of many fastidious organisms.

The original formulation included 0.3g dextrose per litre which interfered with the haemolytic reactions. It is now used without dextrose as a standard medium³.

Tryptose Blood Agar Base with added blood gives good haemolytic reactions and without blood it will sustain good to excellent growth of many demanding organisms. However, to improve the growth of certain organisms e.g. Neisseria meningitidis and Streptococcus pneumoniae, the addition of 1g of yeast extract (LP0021) to one litre of Tryptose Blood Agar Base can be made.

Technique
Lightly inoculate the surface of the medium and incubate aerobically at 35°C for 18-24 hours in 5-10% carbon dioxide (if necessary) or incubate anaerobically at 35°C for 48 hours to enhance haemolysis and to restrict unwanted commensal growth.

Carry out microscopy and other identification tests on the isolated colonies.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel

方向
将 30 克悬浮在 1 升蒸馏水中。煮沸以完全溶解。通过在 121°C 高压灭菌 15 分钟进行灭菌。

对于血琼脂，将基础培养基冷却至 45-50°C 并加入 7% 的无菌血液。彻底混合，注意避免混入气泡，然后分装到培养皿或其他容器中。

描述
Casman1,2 专门开发的一种高营养培养基，用于制备血琼脂，支持许多挑剔微生物的生长。

最初的配方包括每升 0.3 克葡萄糖，它会干扰溶血反应。现在它在没有葡萄糖的情况下用作标准培养基 3。

添加血液的胰蛋白酶血琼脂基质可产生良好的溶血反应，而在没有血液的情况下，它将维持许多高要求生物体的良好生长。但是，为了改善某些生物体的生长，例如脑膜炎奈瑟氏菌和肺炎链球菌，将 1g 酵母提取物 (LP0021) 添加到 1 升胰蛋白酶血琼脂培养基中即可制成。

技术
轻轻接种培养基表面并在 5-10% 二氧化碳（如有必要）中 35°C 有氧孵育 18-24 小时或在 35°C 无氧孵育 48 小时以增强溶血并限制不需要的共生生长。

对分离的菌落进行显微镜检查和其他鉴定测试。

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的培养基板储存在 2-8°C。

外貌
脱水介质：稻草色、自由流动的粉末
制备培养基：稻草色凝胶

Quality control

* This organism is available as a Culti-Loop®

References
1. Casman E. P. (1942) J. Bact. 43. 33-37.
2. Casman E. P. (1947) Amer. J. Clin. Path. 17. 281-282.
3. American Public Health Association (1970) Diagnostic Procedures and Reagents. 5th Edn. APHA Inc. New York.