标签归档：yellow

CAS NO：2390-54-7 Thioflavin T ( Basic Yellow 1) 硫黄素T（碱性黄1）

发表于由

Thioflavin T硫黄素T；Basic Yellow 1 碱性黄1；Thioflavin S 硫黄素S；Amyloid plaques 淀粉样斑块；βsheet； CAS NO：2390-54-7；

产品信息

产品名称 规格
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） 1g
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） 5g
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） 25g

产品描述

硫黄素T（Thioflavin T），又称为碱性黄1（Basic Yellow 1），是一种具细胞膜渗透性的苯并噻唑染料，常用来鉴定样本内淀粉样纤维的存在与否。一旦与淀粉样纤维结合后荧光信号明显增强，结合后引起最大激发波长（385nm→450nm）和最大发射波长（445nm→482nm）的迁移。硫黄素T能用来监测堆积的β折叠，也能用来组织学和蛋白质鉴定。

产品特性

1） CAS NO.：2390-54-7

2） 化学名：4-(3,6-Dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylaniline chloride

3） 同义名：Basic Yellow 1; C.I. 49005; ThT; 碱性黄1；硫代磺素T；硫代黄色素 T；

4） 分子式：C17H19ClN2S

5） 分子量：318.86

6） 纯度：≥97%

7） 外观：黄色粉末

8） 溶解性：溶于DMSO、DMF、H2O（5mg/ml）

9） 化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，2年有效。

运输：室温运输。

应用示例

1、文献来源：Khurana R et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38.

示例1：使用ThT的通用实验技术。a) Structure of ThT (top). The two planer segments of ThT whose mutual rotation defines chirality are also shown (bottom).(b) Early histology using Thioflavin-T to stain primary kidney amyloid.(c) TIRF microscopy image of branched glucagon fibrils stained with ThT.(d) Characteristic increase in ThT upon binding to amyloid fibrils.(e) Fibrillization kinetics of increased concentrations of a fibril-forming peptide. The rapid onset of fibrillization induced through seeding is also shown.

2、文献来源：Huang DS et al. Protective Effects of Wogonin against Alzheimer’s Disease by Inhibition of Amyloidogenic Pathway. Evid Based Complement Alternat Med. 2017;2017:3545169.

Thioflavin T Assay（in solution）：The freshly prepared Aβ42 peptides (20 μM) with/without wogonin (10 μM) were added in a 100 μL sample containing 50 mM glycine and 40 μM Thioflavin T. The samples were loaded in a 96-well plate and florescent intensity of each sample was measured by excitation of 440 nm and emission of 490 mm every 30 min at 37°C for 16 hours.

3、文献来源：Marathe S et al. Jagged1 Is Altered in Alzheimer’s Disease and Regulates Spatial Memory Processing. Front Cell Neurosci. 2017 Aug 9;11:220.

Jagged1 expression in brains and CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) and Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls and AD patients (A-C). In the healthy controls, Jagged1 is localized to the somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) and (C,C′) core plaques double positive for Notch1 and Thioflavin-T. [Misfolded β-sheets were stained with Thioflavin-T at a concentration of 100 mM diluted in water]

注意事项

1） 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

Lucifer Yellow Cadaverine 萤光黄尸胺 Nile Red 尼罗红 FluoroGold CAS NO：149733-79-9

发表于由

Lucifer Yellow Cadaverine 萤光黄尸胺；Lucifer Yellow CH；萤光黄CH; Polar molecular tracer 极性分子示踪剂；神经元形态学；Nile Red 尼罗红；FluoroGold；CAS NO：149733-79-9；

产品信息

产品名称

 产品编号 规格 CAS NO.
Lucifer Yellow Cadaverine 萤光黄尸胺 MX4477-10MG 10mg 149733-79-9

产品描述

萤光黄尸胺（Lucifer Yellow Cadaverine），和萤光黄CH（Lucifer Yellow CH）类似，是一种可固定的荧光示踪剂。另外，萤光黄尸胺还能用于偶联羧酸基团。

产品特性

CAS NO.：149733-79-9 分子式：C17H17K2N3O8S2
分子量：534 荧光：λEx/λ Em = 428/536 nm
外观：黄色固体 溶解性：溶于H2O
化学结构式：

保存与运输方法

保存：≤4ºC避光干燥保存，至少2年有效。 

运输：冰袋运输。

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

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MX4231-10MG Hydroxystilbamidine (FluoroGold) 羟芪巴脒（荧光金） 10mg
MX4461-25MG Sulforhodamine 101 磺酰罗丹明101 25mg
MX4475-25MG Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐 25mg
MX4476-25MG Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐 25mg
MX4477-10MG Lucifer Yellow Cadaverine 萤光黄尸胺 10mg
MX4478-25MG Biocytin (ε-Biotinoyl-L-lysine) 生物胞素 25mg
MX4479-25MG Biocytin Hydrazide 生物胞素酰肼 25mg
MX4480-25MG Biotin Ethylenediamine Hydrobromide 生物素乙二胺氢溴酸 25mg

Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐 FluoroGold CAS NO：71206-95-6

发表于由

Lucifer Yellow CH；萤光黄CH; Polar molecular tracer 极性分子示踪剂；神经元形态学；Nile Red 尼罗红；FluoroGold；CAS NO：71206-95-6；

产品信息

产品名称

 产品编号 规格 CAS NO.

Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐

 MX4476-25MG 25mg 71206-95-6

Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐 MX4476-100MG 100mg 71206-95-6

产品描述

萤光黄CH钾盐（Lucifer Yellow CH Potassium salt）是一种强荧光染料，用于标记神经细胞和示踪细胞间的连接。与萤光黄VS（Lucifer Yellow VS）相比，萤光黄CH包含一个碳酰肼基团，在醛类固定过程中能共价结合到周围的生物分子上。萤光黄CH钾盐能用于可视化细胞形态，以及细胞内标记记录神经元。这种极性示踪剂和其它相似的非膜渗透性染料往往通过微注射、胞饮作用、划痕标记、ATP诱导的跨膜或渗透压冲击来完成加载。

锂盐形式的萤光黄CH（Lucifer Yellow CH potassium salt，Cat#：MX4475）通常用于微注射，因其比钾盐或铵盐形式的萤光黄CH表现出更高的溶解性。钾盐或铵盐形式的萤光黄CH倾向于用在锂离子可能干扰某些生物功能的场景。

产品特性

1）CAS NO.：71206-95-6

2）化学名：6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid, dipotassium salt

3）同义名：Lucifer Yellow carbohydrazide potassium salt; Lucifer Yellow CH dipotassium salt; 萤光黄碳酰肼钾盐；萤光黄CH二钾盐；萤黄CH二钾盐；

4）分子式：C13H9K2N5O9S2

5）分子量：521.57

6）荧光：λEx/λ Em = 428/536 nm

7）外观：固体

8）溶解性：溶于H2O（~1mg/ml）

9）化学结构式：

保存与运输方法

保存：≤4ºC避光干燥保存，至少2年有效。 

运输：冰袋运输。

应用示例（来自文献，仅作参考）

文献2：Li Cao, Yunlin Chen, Li Lu, Yihao Liu, Yaowen Wang, Jinqi Fan, Yuehui Yin, Angiotensin II upregulates fibroblast-myofibroblast transition through Cx43-dependent CaMKII and TGF-β1 signaling in neonatal rat cardiac fibroblasts, Acta Biochimica et Biophysica Sinica, Volume 50, Issue 9, September 2018, Pages 843–852, https://doi.org/10.1093/abbs/gmy090

实验目的：染料转运实验（Dye transfer assay）

实验方法：萤光黄染料能进入受损细胞，通过功能性的缝隙连接在相邻细胞间转运。染料转运距离反映间隙连接细胞间通讯（GJIC）的功能状态。经处理后，完全长满的成纤维细胞用PBS清洗，用枪头进行划痕，之后孵育在含0.5%萤光黄CH钾盐的PBS溶液，37℃孵育5min。之后用PBS清洗3次，4%多聚甲醛固定，荧光显微镜拍照成像。

Fig. Ang-(1–7) and AIP reversed CaMKII activation and upregulation of α-SMA, TGF-β1 and Cx43 in Ang II-induced neonatal rat cardiac fibroblasts (A) Representative immunohistochemistry images of α-SMA (green) and Cx43 (red), and quantification of integrated density of α-SMA and Cx43 (right). Scale bars = 30 μm. (B,C) Western blot analysis of p-CaMKII, ox-CaMKII, CaMKII, α-SMA, TGF-β1 and Cx43, and quantification of the protein bands (right). (D) Fluorescence images showing Lucifer yellow dye transfer after scrape loading. n = 3. *P < 0.05 versus the control group; #P < 0.05 versus the Ang II group.

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号 名称 规格
MX4231-10MG Hydroxystilbamidine (FluoroGold) 羟芪巴脒（荧光金） 10mg
MX4461-25MG Sulforhodamine 101 磺酰罗丹明101 25mg
MX4475-25MG Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐 25mg
MX4476-25MG Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐 25mg
MX4477-10MG Lucifer Yellow Cadaverine 萤光黄尸胺 10mg
MX4478-25MG Biocytin (ε-Biotinoyl-L-lysine) 生物胞素 25mg
MX4479-25MG Biocytin Hydrazide 生物胞素酰肼 25mg
MX4480-25MG Biotin Ethylenediamine Hydrobromide 生物素乙二胺氢溴酸 25mg

Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐

发表于由

Lucifer Yellow CH；萤光黄CH; Polar molecular tracer 极性分子示踪剂；神经元形态学；Nile Red 尼罗红；FluoroGold；CAS NO：71206-95-6；

产品信息

产品名称

 产品编号 规格 CAS NO.

Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐

 MX4476-25MG 25mg 71206-95-6

Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐 MX4476-100MG 100mg 71206-95-6

产品描述

萤光黄CH钾盐（Lucifer Yellow CH Potassium salt）是一种强荧光染料，用于标记神经细胞和示踪细胞间的连接。与萤光黄VS（Lucifer Yellow VS）相比，萤光黄CH包含一个碳酰肼基团，在醛类固定过程中能共价结合到周围的生物分子上。萤光黄CH钾盐能用于可视化细胞形态，以及细胞内标记记录神经元。这种极性示踪剂和其它相似的非膜渗透性染料往往通过微注射、胞饮作用、划痕标记、ATP诱导的跨膜或渗透压冲击来完成加载。

锂盐形式的萤光黄CH（Lucifer Yellow CH potassium salt，Cat#：MX4475）通常用于微注射，因其比钾盐或铵盐形式的萤光黄CH表现出更高的溶解性。钾盐或铵盐形式的萤光黄CH倾向于用在锂离子可能干扰某些生物功能的场景。

产品特性

1）CAS NO.：71206-95-6

2）化学名：6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid, dipotassium salt

3）同义名：Lucifer Yellow carbohydrazide potassium salt; Lucifer Yellow CH dipotassium salt; 萤光黄碳酰肼钾盐；萤光黄CH二钾盐；萤黄CH二钾盐；

4）分子式：C13H9K2N5O9S2

5）分子量：521.57

6）荧光：λEx/λ Em = 428/536 nm

7）外观：固体

8）溶解性：溶于H2O（~1mg/ml）

9）化学结构式：

保存与运输方法

保存：≤4ºC避光干燥保存，至少2年有效。 

运输：冰袋运输。

应用示例（来自文献，仅作参考）

文献2：Li Cao, Yunlin Chen, Li Lu, Yihao Liu, Yaowen Wang, Jinqi Fan, Yuehui Yin, Angiotensin II upregulates fibroblast-myofibroblast transition through Cx43-dependent CaMKII and TGF-β1 signaling in neonatal rat cardiac fibroblasts, Acta Biochimica et Biophysica Sinica, Volume 50, Issue 9, September 2018, Pages 843–852, https://doi.org/10.1093/abbs/gmy090

实验目的：染料转运实验（Dye transfer assay）

实验方法：萤光黄染料能进入受损细胞，通过功能性的缝隙连接在相邻细胞间转运。染料转运距离反映间隙连接细胞间通讯（GJIC）的功能状态。经处理后，完全长满的成纤维细胞用PBS清洗，用枪头进行划痕，之后孵育在含0.5%萤光黄CH钾盐的PBS溶液，37℃孵育5min。之后用PBS清洗3次，4%多聚甲醛固定，荧光显微镜拍照成像。

Fig. Ang-(1–7) and AIP reversed CaMKII activation and upregulation of α-SMA, TGF-β1 and Cx43 in Ang II-induced neonatal rat cardiac fibroblasts (A) Representative immunohistochemistry images of α-SMA (green) and Cx43 (red), and quantification of integrated density of α-SMA and Cx43 (right). Scale bars = 30 μm. (B,C) Western blot analysis of p-CaMKII, ox-CaMKII, CaMKII, α-SMA, TGF-β1 and Cx43, and quantification of the protein bands (right). (D) Fluorescence images showing Lucifer yellow dye transfer after scrape loading. n = 3. *P < 0.05 versus the control group; #P < 0.05 versus the Ang II group.

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号 名称 规格
MX4231-10MG Hydroxystilbamidine (FluoroGold) 羟芪巴脒（荧光金） 10mg
MX4461-25MG Sulforhodamine 101 磺酰罗丹明101 25mg
MX4475-25MG Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐 25mg
MX4476-25MG Lucifer Yellow CH (Potassium salt) 萤光黄CH钾盐 25mg
MX4477-10MG Lucifer Yellow Cadaverine 萤光黄尸胺 10mg
MX4478-25MG Biocytin (ε-Biotinoyl-L-lysine) 生物胞素 25mg
MX4479-25MG Biocytin Hydrazide 生物胞素酰肼 25mg
MX4480-25MG Biotin Ethylenediamine Hydrobromide 生物素乙二胺氢溴酸 25mg

Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐

发表于由

Lucifer Yellow CH；萤光黄CH;Polar molecular tracer 极性分子示踪剂；神经元形态学；Nile Red 尼罗红；FluoroGold；CAS NO：67769-47-5；

产品信息

产品名称

 产品编号 规格 CAS NO.

Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐  

 MX4475-25MG     25mg 67769-47-5

Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐 MX4475-100MG 100mg 67769-47-5

产品描述

萤光黄CH（Lucifer Yellow CH, LY-CH）是一种强荧光染料，长期被用于标记神经细胞。与萤光黄VS（Lucifer Yellow VS）相比，萤光黄CH包含一个碳酰肼基团，在醛类固定过程中能共价结合到周围的生物分子上。作为一种生物示踪剂，来监测神经元的分支形态和再生、间隙连接和鉴定，以及醛类固定后的选择性细胞消融。这种极性示踪剂和其它相似的非膜渗透性染料往往通过微注射、胞饮作用、划痕标记、ATP诱导的跨膜或渗透压冲击来完成加载。

本品为锂盐形式的萤光黄CH（Lucifer Yellow CH lithium salt），通常用于微注射，因其比钾盐形式的萤光黄CH表现出更高的溶解性。钾盐形式的萤光黄CH（Lucifer Yellow CH potassium salt）倾向于用在锂离子可能干扰某些生物功能的场景。

产品特性

1）CAS NO.：67769-47-5

2）化学名：6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid, dilithium salt

3）同义名：Lucifer Yellow carbohydrazide lithium salt; Lucifer Yellow CH dilithium salt; 萤光黄碳酰肼锂盐；萤光黄CH二锂盐；萤黄CH二锂盐；

4）分子式：C13H9Li2N5O9S2

5）分子量：457.25

6）荧光：λex 428 nm; λem 540 nm in H2O

7）外观：固体

8）溶解性：溶于H2O（1mg/ml）

9）化学结构式：

保存与运输方法

保存：≤4ºC避光干燥保存，至少2年有效。

运输：冰袋运输。

应用示例（来自文献，仅作参考）

文献1：Bederska M, Borucki W, Znojek E. Movement of fluorescent dyes Lucifer Yellow (LYCH) and carboxyfluorescein (CF) in Medicago truncatula Gaertn. roots and root nodules. Symbiosis. 2012;58(1-3):183-190. doi:10.1007/s13199-013-0221-7

实验目的：萤光黄进行质外体示踪（apoplastic tracing）

实验方法：根瘤暴露于含1%萤光黄的溶液12h，之后不经固定或经2次1min间隔真空抽吸和4%戊二醛过夜固定。处理后根部和根瘤用去离子水清洗。切片用激光扫描共聚焦显微镜观察（405nm二极管激光器激发，发射光谱为500nm-550nm之间，最大发射波长为528nm）。

 

Fig. The bright field (a) and CLSM (b–g) photographs of non-fixed tissues. Part of nodulated root system (a). Cross section of control root nodule (b). The uptake of LYCH by untouched root (c) and untouched root nodule (d, e – cross section). Damaged root exhibited LYCH fluorescence in xylem elements (d,g). Cross section of the stele of control root (f). Cross section of root stele treated with LYCH (g). Abbreviations: IT – inner tissue, OC – outer cortex, VB – vascular bundle, X – xylem vessels, arrow – parts of cells stained with LYCH, double arrow-head – autofluorescence of uninfected cells. Bars represent: A and C= 100 μm, B and E= 200 μm, D= 150 μm, F and G= 50 μm. LYCH fluorescence was observed after excitation at 405 nm, emission spectrum was between 500 nm and 550 nm

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号 名称 规格         
MX4231-10MG       Hydroxystilbamidine (FluoroGold)羟芪巴脒（荧光金） 10mg
MX4461-25MG Sulforhodamine 101磺酰罗丹明101 25mg
MX4475-25MG Lucifer Yellow CH (Lithium salt)萤光黄CH锂盐 25mg
MX4476-25MG Lucifer Yellow CH (Potassium salt)萤光黄CH钾盐 25mg
MX4477-10MG Lucifer Yellow Cadaverine萤光黄尸胺 10mg
MX4478-25MG Biocytin (ε-Biotinoyl-L-lysine)生物胞素 25mg
MX4479-25MG Biocytin Hydrazide生物胞素酰肼 25mg
MX4480-25MG Biotin Ethylenediamine Hydrobromide生物素乙二胺氢溴酸    25mg

 

Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐 FluoroGold CAS NO：67769-47-5

发表于由

Lucifer Yellow CH；萤光黄CH;Polar molecular tracer 极性分子示踪剂；神经元形态学；Nile Red 尼罗红；FluoroGold；CAS NO：67769-47-5；

产品信息

产品名称

 产品编号 规格 CAS NO.

Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐  

 MX4475-25MG     25mg 67769-47-5

Lucifer Yellow CH (Lithium salt) 萤光黄CH锂盐 MX4475-100MG 100mg 67769-47-5

产品描述

萤光黄CH（Lucifer Yellow CH, LY-CH）是一种强荧光染料，长期被用于标记神经细胞。与萤光黄VS（Lucifer Yellow VS）相比，萤光黄CH包含一个碳酰肼基团，在醛类固定过程中能共价结合到周围的生物分子上。作为一种生物示踪剂，来监测神经元的分支形态和再生、间隙连接和鉴定，以及醛类固定后的选择性细胞消融。这种极性示踪剂和其它相似的非膜渗透性染料往往通过微注射、胞饮作用、划痕标记、ATP诱导的跨膜或渗透压冲击来完成加载。

本品为锂盐形式的萤光黄CH（Lucifer Yellow CH lithium salt），通常用于微注射，因其比钾盐形式的萤光黄CH表现出更高的溶解性。钾盐形式的萤光黄CH（Lucifer Yellow CH potassium salt）倾向于用在锂离子可能干扰某些生物功能的场景。

产品特性

1）CAS NO.：67769-47-5

2）化学名：6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid, dilithium salt

3）同义名：Lucifer Yellow carbohydrazide lithium salt; Lucifer Yellow CH dilithium salt; 萤光黄碳酰肼锂盐；萤光黄CH二锂盐；萤黄CH二锂盐；

4）分子式：C13H9Li2N5O9S2

5）分子量：457.25

6）荧光：λex 428 nm; λem 540 nm in H2O

7）外观：固体

8）溶解性：溶于H2O（1mg/ml）

9）化学结构式：

保存与运输方法

保存：≤4ºC避光干燥保存，至少2年有效。

运输：冰袋运输。

应用示例（来自文献，仅作参考）

文献1：Bederska M, Borucki W, Znojek E. Movement of fluorescent dyes Lucifer Yellow (LYCH) and carboxyfluorescein (CF) in Medicago truncatula Gaertn. roots and root nodules. Symbiosis. 2012;58(1-3):183-190. doi:10.1007/s13199-013-0221-7

实验目的：萤光黄进行质外体示踪（apoplastic tracing）

实验方法：根瘤暴露于含1%萤光黄的溶液12h，之后不经固定或经2次1min间隔真空抽吸和4%戊二醛过夜固定。处理后根部和根瘤用去离子水清洗。切片用激光扫描共聚焦显微镜观察（405nm二极管激光器激发，发射光谱为500nm-550nm之间，最大发射波长为528nm）。

Fig. The bright field (a) and CLSM (b–g) photographs of non-fixed tissues. Part of nodulated root system (a). Cross section of control root nodule (b). The uptake of LYCH by untouched root (c) and untouched root nodule (d, e – cross section). Damaged root exhibited LYCH fluorescence in xylem elements (d,g). Cross section of the stele of control root (f). Cross section of root stele treated with LYCH (g). Abbreviations: IT – inner tissue, OC – outer cortex, VB – vascular bundle, X – xylem vessels, arrow – parts of cells stained with LYCH, double arrow-head – autofluorescence of uninfected cells. Bars represent: A and C= 100 μm, B and E= 200 μm, D= 150 μm, F and G= 50 μm. LYCH fluorescence was observed after excitation at 405 nm, emission spectrum was between 500 nm and 550 nm

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

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MX4461-25MG Sulforhodamine 101磺酰罗丹明101 25mg
MX4475-25MG Lucifer Yellow CH (Lithium salt)萤光黄CH锂盐 25mg
MX4476-25MG Lucifer Yellow CH (Potassium salt)萤光黄CH钾盐 25mg
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Hoechst染料（Hoechst dye）是一个DNA标记用荧光染料家族，用于荧光成像和流式分析

发表于由

Nuclear Yellow 核黄；Hoechst S769121；Fluoro-Gold™；Fluoro-ruby；Neuronal retrograde tracer；神经元逆行示踪剂；Anterograde tracer 顺行示踪剂；CAS：74681-68-8；

订购信息：

货号

 产品名称 CAS NO.           规格                  
MX4232-5MG  Nuclear Yellow (Hoechst S769121) 核黄   74681-68-8 5mg
MX4232-25MG      Nuclear Yellow (Hoechst S769121) 核黄      74681-68-8 25mg

Hoechst染料（Hoechst dye）是一个DNA标记用荧光染料家族，用于荧光成像和流式分析。由于这类荧光染料能标记DNA，通常用于观察核酸和线粒体。

核黄（Nuclear Yellow），又称为Hoechst S769121，是长波长示踪剂，常常与广受欢迎的逆行示踪剂真蓝（True Blue）联合使用做双色神经元映射。神经元细胞内，核黄主要染细胞核，呈黄色荧光，而真蓝是紫外激发的二价阳离子染料，染细胞质，呈蓝色荧光。两种染料对免疫组化处理过程都很稳定，且能用在图片转化DAB为不可溶、电子致密的反应产物。

产品特性

  1. CAS NO.：74681-68-8
  2. 同义名：Hoechst S769121
  3. 分子式：C25H28Cl3N7O2S
  4. 分子量：596.96
  5. 外观：浅黄色粉末
  6. 纯度：≥98%（HPLC）
  7. 溶解性：溶于H2O或DMSO
  8. Ex/Em：355/495nm
  9. 化学结构式：  

保存与运输方法

保存：-20℃干燥避光保存，有效期至少2年。

运输：冰袋运输。

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

操作流程（细胞染色）

以下步骤适用于绝大多数细胞类型。生长培养基、细胞密度、是否存在其他细胞类型和其他因素都可能影响染色。玻璃器皿上残留的去污剂也可能会影响真实或许多有机体的表观染色，导致含或不含细胞的溶液中出现明亮染色的材料。

1）离心收集细胞。

2）在缓冲溶液或培养基（pH 7.4）中重悬细胞。

3）在盖玻片上或细胞培养孔内原位染贴壁细胞。

4）加Hoechst染料使其浓度为0.5-5µM。

5）孵育细胞15-60min。

【注意】：孵育时间和染料浓度需通过实验来确定。最初实验需要在整个建议范围内尝试几个染料浓度以确定能得到最佳染色的工作浓度。

相关产品：

货号 名称 规格
MX4231-10MG Hydroxystilbamidine羟芪巴脒 10mg
MX4232-25MG Nuclear Yellow (Hoechst S769121)核黄 25mg
MX4014-1MG FM 1-43膜电位荧光探针 1mg
MX4015-1MG FM 2-10膜电位荧光探针 1mg
MX4016-1MG FM 4-64膜电位荧光探针 1mg
MX4019-5MG RH 414膜电位荧光探针 5mg
MX4020-1MG RH 795膜电位荧光探针 1mg
MX4017-5MG RH 237膜电位荧光探针 5mg
MX4018-5MG RH 421膜电位荧光探针 5mg
MX4205-10MG             Propidium Iodide碘化丙啶（粉末） 10mg                  
MX4205-1ML Propidium Iodide (1mg/ml)碘化丙啶（1mg/ml）                 1ml
MF0756-1G Ethidium Bromide (EB)溴化乙锭 1g
MF0756-5ML EB (10 mg/ml in Water) EB水溶液（10 mg/ml） 5ml

Nuclear Yellow (Hoechst S769121) 核黄 Anterograde tracer 顺行示踪剂 CAS：74681-68-8

发表于由

Nuclear Yellow 核黄；Hoechst S769121；Fluoro-Gold™；Fluoro-ruby；Neuronal retrograde tracer；神经元逆行示踪剂；Anterograde tracer 顺行示踪剂；CAS：74681-68-8；

订购信息：

货号

 产品名称 CAS NO.           规格                   
MX4232-5MG  Nuclear Yellow (Hoechst S769121) 核黄   74681-68-8 5mg
MX4232-25MG      Nuclear Yellow (Hoechst S769121) 核黄      74681-68-8 25mg

Hoechst染料（Hoechst dye）是一个DNA标记用荧光染料家族，用于荧光成像和流式分析。由于这类荧光染料能标记DNA，通常用于观察核酸和线粒体。

核黄（Nuclear Yellow），又称为Hoechst S769121，是长波长示踪剂，常常与广受欢迎的逆行示踪剂真蓝（True Blue）联合使用做双色神经元映射。神经元细胞内，核黄主要染细胞核，呈黄色荧光，而真蓝是紫外激发的二价阳离子染料，染细胞质，呈蓝色荧光。两种染料对免疫组化处理过程都很稳定，且能用在图片转化DAB为不可溶、电子致密的反应产物。

产品特性

  1. CAS NO.：74681-68-8
  2. 同义名：Hoechst S769121
  3. 分子式：C25H28Cl3N7O2S
  4. 分子量：596.96
  5. 外观：浅黄色粉末
  6. 纯度：≥98%（HPLC）
  7. 溶解性：溶于H2O或DMSO
  8. Ex/Em：355/495nm

保存与运输方法

保存：-20℃干燥避光保存，有效期至少2年。

运输：冰袋运输。

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

操作流程（细胞染色）

以下步骤适用于绝大多数细胞类型。生长培养基、细胞密度、是否存在其他细胞类型和其他因素都可能影响染色。玻璃器皿上残留的去污剂也可能会影响真实或许多有机体的表观染色，导致含或不含细胞的溶液中出现明亮染色的材料。

1）离心收集细胞。

2）在缓冲溶液或培养基（pH 7.4）中重悬细胞。

3）在盖玻片上或细胞培养孔内原位染贴壁细胞。

4）加Hoechst染料使其浓度为0.5-5µM。

5）孵育细胞15-60min。

【注意】：孵育时间和染料浓度需通过实验来确定。最初实验需要在整个建议范围内尝试几个染料浓度以确定能得到最佳染色的工作浓度。

相关产品：

货号 名称 规格
MX4231-10MG Hydroxystilbamidine羟芪巴脒 10mg
MX4232-25MG Nuclear Yellow (Hoechst S769121)核黄 25mg
MX4014-1MG FM 1-43膜电位荧光探针 1mg
MX4015-1MG FM 2-10膜电位荧光探针 1mg
MX4016-1MG FM 4-64膜电位荧光探针 1mg
MX4019-5MG RH 414膜电位荧光探针 5mg
MX4020-1MG RH 795膜电位荧光探针 1mg
MX4017-5MG RH 237膜电位荧光探针 5mg
MX4018-5MG RH 421膜电位荧光探针 5mg
MX4205-10MG             Propidium Iodide碘化丙啶（粉末） 10mg                  
MX4205-1ML Propidium Iodide (1mg/ml)碘化丙啶（1mg/ml）                 1ml
MF0756-1G Ethidium Bromide (EB)溴化乙锭 1g
MF0756-5ML EB (10 mg/ml in Water) EB水溶液（10 mg/ml） 5ml

Thioflavin T ( Basic Yellow 1) 硫黄素T（碱性黄1）

发表于由

描述

Thioflavin T ( Basic Yellow 1) 硫黄素T（碱性黄1）

产品关键词：

Thioflavin T硫黄素T；Basic Yellow 1 碱性黄1；Thioflavin S 硫黄素S；Amyloid plaques 淀粉样斑块；βsheet； CAS NO：2390-54-7；

产品信息

产品名称 产品编号 规格 价格（元）
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） MX4464-1G 1g 180
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） MX4464-5G 5g 380
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） MX4464-25G 25g 1080

产品描述

硫黄素T（Thioflavin T），又称为碱性黄1（Basic Yellow 1），是一种具细胞膜渗透性的苯并噻唑染料，常用来鉴定样本内淀粉样纤维的存在与否。一旦与淀粉样纤维结合后荧光信号明显增强，结合后引起最大激发波长（385nm→450nm）和最大发射波长（445nm→482nm）的迁移。硫黄素T能用来监测堆积的β折叠，也能用来组织学和蛋白质鉴定。

产品特性

1） CAS NO.：2390-54-7

2） 化学名：4-(3,6-Dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylaniline chloride

3） 同义名：Basic Yellow 1; C.I. 49005; ThT; 碱性黄1；硫代磺素T；硫代黄色素 T；

4） 分子式：C17H19ClN2S

5） 分子量：318.86

6） 纯度：≥97%

7） 外观：黄色粉末

8） 溶解性：溶于DMSO、DMF、H2O（5mg/ml）

9） 化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，2年有效。

运输：室温运输。

应用示例

1、文献来源：Khurana R et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38.

示例1：使用ThT的通用实验技术。a) Structure of ThT (top). The two planer segments of ThT whose mutual rotation defines chirality are also shown (bottom).(b) Early histology using Thioflavin-T to stain primary kidney amyloid.(c) TIRF microscopy image of branched glucagon fibrils stained with ThT.(d) Characteristic increase in ThT upon binding to amyloid fibrils.(e) Fibrillization kinetics of increased concentrations of a fibril-forming peptide. The rapid onset of fibrillization induced through seeding is also shown.

2、文献来源：Huang DS et al. Protective Effects of Wogonin against Alzheimer’s Disease by Inhibition of Amyloidogenic Pathway. Evid Based Complement Alternat Med. 2017;2017:3545169.

Thioflavin T Assay（in solution）：The freshly prepared Aβ42 peptides (20 μM) with/without wogonin (10 μM) were added in a 100 μL sample containing 50 mM glycine and 40 μM Thioflavin T. The samples were loaded in a 96-well plate and florescent intensity of each sample was measured by excitation of 440 nm and emission of 490 mm every 30 min at 37°C for 16 hours.

3、文献来源：Marathe S et al. Jagged1 Is Altered in Alzheimer’s Disease and Regulates Spatial Memory Processing. Front Cell Neurosci. 2017 Aug 9;11:220.

Jagged1 expression in brains and CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) and Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls and AD patients (A-C). In the healthy controls, Jagged1 is localized to the somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) and (C,C′) core plaques double positive for Notch1 and Thioflavin-T. [Misfolded β-sheets were stained with Thioflavin-T at a concentration of 100 mM diluted in water]

注意事项

1） 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

Thioflavin T ( Basic Yellow 1) 硫黄素T（碱性黄1）

发表于由

描述

Thioflavin T ( Basic Yellow 1) 硫黄素T（碱性黄1）

产品关键词：

Thioflavin T硫黄素T；Basic Yellow 1 碱性黄1；Thioflavin S 硫黄素S；Amyloid plaques 淀粉样斑块；βsheet； CAS NO：2390-54-7；

产品信息

产品名称 产品编号 规格 价格（元）
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） MX4464-1G 1g 180
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） MX4464-5G 5g 380
Thioflavin T ( Basic Yellow 1)硫黄素T（碱性黄1） MX4464-25G 25g 1080

产品描述

硫黄素T（Thioflavin T），又称为碱性黄1（Basic Yellow 1），是一种具细胞膜渗透性的苯并噻唑染料，常用来鉴定样本内淀粉样纤维的存在与否。一旦与淀粉样纤维结合后荧光信号明显增强，结合后引起最大激发波长（385nm→450nm）和最大发射波长（445nm→482nm）的迁移。硫黄素T能用来监测堆积的β折叠，也能用来组织学和蛋白质鉴定。

产品特性

1） CAS NO.：2390-54-7

2） 化学名：4-(3,6-Dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylaniline chloride

3） 同义名：Basic Yellow 1; C.I. 49005; ThT; 碱性黄1；硫代磺素T；硫代黄色素 T；

4） 分子式：C17H19ClN2S

5） 分子量：318.86

6） 纯度：≥97%

7） 外观：黄色粉末

8） 溶解性：溶于DMSO、DMF、H2O（5mg/ml）

9） 化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，2年有效。

运输：室温运输。

应用示例

1、文献来源：Khurana R et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38.

示例1：使用ThT的通用实验技术。a) Structure of ThT (top). The two planer segments of ThT whose mutual rotation defines chirality are also shown (bottom).(b) Early histology using Thioflavin-T to stain primary kidney amyloid.(c) TIRF microscopy image of branched glucagon fibrils stained with ThT.(d) Characteristic increase in ThT upon binding to amyloid fibrils.(e) Fibrillization kinetics of increased concentrations of a fibril-forming peptide. The rapid onset of fibrillization induced through seeding is also shown.

2、文献来源：Huang DS et al. Protective Effects of Wogonin against Alzheimer’s Disease by Inhibition of Amyloidogenic Pathway. Evid Based Complement Alternat Med. 2017;2017:3545169.

Thioflavin T Assay（in solution）：The freshly prepared Aβ42 peptides (20 μM) with/without wogonin (10 μM) were added in a 100 μL sample containing 50 mM glycine and 40 μM Thioflavin T. The samples were loaded in a 96-well plate and florescent intensity of each sample was measured by excitation of 440 nm and emission of 490 mm every 30 min at 37°C for 16 hours.

3、文献来源：Marathe S et al. Jagged1 Is Altered in Alzheimer’s Disease and Regulates Spatial Memory Processing. Front Cell Neurosci. 2017 Aug 9;11:220.

Jagged1 expression in brains and CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) and Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls and AD patients (A-C). In the healthy controls, Jagged1 is localized to the somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) and (C,C′) core plaques double positive for Notch1 and Thioflavin-T. [Misfolded β-sheets were stained with Thioflavin-T at a concentration of 100 mM diluted in water]

注意事项

1） 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

Fluorol Yellow 088 荧光黄088

发表于由

描述

Fluorol Yellow 088 荧光黄088

产品标签

Fluorol Yellow 088 荧光黄088；Solvent Green 4 溶绿4；Suberin lamellae 木栓质层；Ruthenium Red 钌红；Calcofluor White Stain 卡尔科弗卢尔荧光增白剂；CAS NO：81-37-8；

产品信息

产品名称

 产品编号 CAS NO.         规格             价格（元）        

Fluorol Yellow 088 荧光黄088       

 MX4473-1G      81-37-8 1g 999
Fluorol Yellow 088 荧光黄088 MX4473-5G 81-37-8 5g

3999
Fluorol Yellow 088 荧光黄088 MX4473-25G 81-37-8 25g

14299

产品描述

荧光黄088（Fluorol Yellow 088）是一种多环芳烃有机染料，一种亲脂性荧光素，用于植物组织木栓质层的细胞染色。

​

产品特性

英文同义名：2,8-Dimethylnaphtho[3,2,1-kl]xanthene; Solvent Green 4; C.I.Fluorescent Brightener 74; Fluorescent Green LF; Fluorol 5G;
中文同义名：2,8-二甲基-萘并[3,2,1-KL]占吨；溶绿4； CAS NO：81-37-8
分子式：C22H16O 分子量：296.36g/mol

纯度：≥95%

荧光：λex 447-453 nm (in methanol)
溶解性：部分溶于水，溶于DMSO，DMF，氯仿和乙酸乙酯 外观：黄色至橙色固体

保存与运输方法

保存：室温避光干燥保存，2年有效。

运输：室温运输。

应用实例

 

文献1:Lux A, Morita S, Abe J, Ito K, 2005.An improved method for clearing and staining free-hand sections and whole-mount samples. Ann Bot (Lond) 96:989–996

A）Clearing solution：Lactic acid was saturated with chloral hydrate.

B）(a) A 0.1 % (w/v) solution of berberine hemisulphate (小檗碱) in lactic acid was dissolved at room temperature. (b) A 0.01 % (w/v) solution of fluorol yellow 088 (荧光黄088, MX4473) in lactic acid was prepared by heating at 70°C for 1h. Due to the low stability of the solution, this was prepared fresh before each use.

C) Clearing and staining procedure

The sections floating in drops of clearing solution on microscope slides were heated over a water bath in covered Petri dishes. Treatment for 1h, using a high temperature (70°C), even with large or dense sections, was usually sufficient. The clearing solution was then absorbed by pipette or tissue paper and the sections were thoroughly washed with distilled water added several times to the sections and absorbed by tissue paper. Sections were post-stained and subsequently washed in the same way. Framing by a liquid blocker (PAP pen) proved useful to prevent the loss of sections from slides.

 

For observation of cell files along the root axis,whole roots were cleared and stained in lactic acid with fluorescence stains (berberine or fluorol yellow) and post-stained with safranin O.This procedure allows observation of epidermal and exodermal cells in thick roots of various species, and in thin roots of arabidopsis it works well for staining and observation of endodermal cells. In thick roots such as those of maize or sorghum, peeling peripheral root tissues exposes the endodermal cells and allows their direct observation. The contrast of cell walls is increased after treatment of peeled samples with berberine in lactic acid.

图1.「D-H」甜瓜不定根的切片经透明处理和含荧光黄088的乳酸染色液染色，在白光「D」、紫外光「E」和两种光下的重叠图片「F」。

文献2: Cohen H et al. A Multilevel Study of Melon Fruit Reticulation Provides Insight into Skin Ligno-Suberization Hallmarks. Plant Physiol. 2019 Apr;179(4):1486-1501.

A）Histochemical observations of suberin and lignin in skin cross sections were achieved by staining with a freshly prepared solution of Fluorol Yellow 088 (0.01% [w/v] in lactic acid) for 30 min at 70°C and phloroglucinol-hydrochloric acid (2% [w/v] in 50% hydrochloric acid) for 30 min at room temperature, respectively. Stained sections were then observed with an Olympus CLSM500 microscope using bright-field and GFP filters. Autofluorescence of cuticle layers in smooth skin sections was observed with a Cy5 filter.

图2.光滑和网状果皮横截面切片的木质素（lignin）（间苯三酚-盐酸染色）和木栓质（suberin）（荧光黄088）染色。

文献3: Gunawardena AH et al. Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae). Am J Bot. 2007 Jul;94(7):1116-28.

A）For fluorol yellow 088, a final concentration of 0.01% (w/v) was made by dissolving 0.01 g of fluorol yellow 088 in 50 mL of PEG 400 solution and heating at 90°C for 1 h (Brundrett et al., 1991). An equal volume of 90% (v/v) glycerol solution was added to the PEG staining solution. Leaves were stained for 1 h at room temperature, rinsed several times with distilled water, and observed with UV light for a bright yellow fluorescence. Unstained cell walls were also examined for autofluorescence as a test for the phenolic constituents of suberin.

图3：成熟花边植物叶的细胞壁组织化学检测。荧光黄088染脂肪族物质「A-D」。透化叶子（A，B）；新鲜叶子（C，D）。微分干涉相衬显微成像图片观察到棕色色素，而荧光成像图片观察到阳性染色（黄绿色荧光）。

注意事项

1）本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

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 名称 规格                

MS4024-25G       

 Aniline Blue (Water Soluble) 苯胺蓝（水溶） 25g
MS4027-5G Toluidine Blue O (TBO) 甲苯胺蓝O 

5g

MS4004A-25G

 Safranine O, BS Grade 番红O（生物染色级） 25g

MS4004B-25G

 Safranine O, High Purity Grade 番红O（高纯级）

25g
MS4074-1G Sudan Red 7B (Solvent Red 19) 苏丹红7B（溶剂红19）       

1g

MX4473-1G

 Fluorol Yellow 088 荧光黄088

1g

 

 

Fluorol Yellow 088 荧光黄088

发表于由

描述

Fluorol Yellow 088 荧光黄088

产品标签

Fluorol Yellow 088 荧光黄088；Solvent Green 4 溶绿4；Suberin lamellae 木栓质层；Ruthenium Red 钌红；Calcofluor White Stain 卡尔科弗卢尔荧光增白剂；CAS NO：81-37-8；

产品信息

产品名称

 产品编号 CAS NO.         规格             价格（元）        

Fluorol Yellow 088 荧光黄088       

 MX4473-1G      81-37-8 1g 999
Fluorol Yellow 088 荧光黄088 MX4473-5G 81-37-8 5g

3999
Fluorol Yellow 088 荧光黄088 MX4473-25G 81-37-8 25g

14299

产品描述

荧光黄088（Fluorol Yellow 088）是一种多环芳烃有机染料，一种亲脂性荧光素，用于植物组织木栓质层的细胞染色。

​

产品特性

英文同义名：2,8-Dimethylnaphtho[3,2,1-kl]xanthene; Solvent Green 4; C.I.Fluorescent Brightener 74; Fluorescent Green LF; Fluorol 5G;
中文同义名：2,8-二甲基-萘并[3,2,1-KL]占吨；溶绿4； CAS NO：81-37-8
分子式：C22H16O 分子量：296.36g/mol

纯度：≥95%

荧光：λex 447-453 nm (in methanol)
溶解性：部分溶于水，溶于DMSO，DMF，氯仿和乙酸乙酯 外观：黄色至橙色固体

保存与运输方法

保存：室温避光干燥保存，2年有效。

运输：室温运输。

应用实例

 

文献1:Lux A, Morita S, Abe J, Ito K, 2005.An improved method for clearing and staining free-hand sections and whole-mount samples. Ann Bot (Lond) 96:989–996

A）Clearing solution：Lactic acid was saturated with chloral hydrate.

B）(a) A 0.1 % (w/v) solution of berberine hemisulphate (小檗碱) in lactic acid was dissolved at room temperature. (b) A 0.01 % (w/v) solution of fluorol yellow 088 (荧光黄088, MX4473) in lactic acid was prepared by heating at 70°C for 1h. Due to the low stability of the solution, this was prepared fresh before each use.

C) Clearing and staining procedure

The sections floating in drops of clearing solution on microscope slides were heated over a water bath in covered Petri dishes. Treatment for 1h, using a high temperature (70°C), even with large or dense sections, was usually sufficient. The clearing solution was then absorbed by pipette or tissue paper and the sections were thoroughly washed with distilled water added several times to the sections and absorbed by tissue paper. Sections were post-stained and subsequently washed in the same way. Framing by a liquid blocker (PAP pen) proved useful to prevent the loss of sections from slides.

 

For observation of cell files along the root axis,whole roots were cleared and stained in lactic acid with fluorescence stains (berberine or fluorol yellow) and post-stained with safranin O.This procedure allows observation of epidermal and exodermal cells in thick roots of various species, and in thin roots of arabidopsis it works well for staining and observation of endodermal cells. In thick roots such as those of maize or sorghum, peeling peripheral root tissues exposes the endodermal cells and allows their direct observation. The contrast of cell walls is increased after treatment of peeled samples with berberine in lactic acid.

图1.「D-H」甜瓜不定根的切片经透明处理和含荧光黄088的乳酸染色液染色，在白光「D」、紫外光「E」和两种光下的重叠图片「F」。

文献2: Cohen H et al. A Multilevel Study of Melon Fruit Reticulation Provides Insight into Skin Ligno-Suberization Hallmarks. Plant Physiol. 2019 Apr;179(4):1486-1501.

A）Histochemical observations of suberin and lignin in skin cross sections were achieved by staining with a freshly prepared solution of Fluorol Yellow 088 (0.01% [w/v] in lactic acid) for 30 min at 70°C and phloroglucinol-hydrochloric acid (2% [w/v] in 50% hydrochloric acid) for 30 min at room temperature, respectively. Stained sections were then observed with an Olympus CLSM500 microscope using bright-field and GFP filters. Autofluorescence of cuticle layers in smooth skin sections was observed with a Cy5 filter.

图2.光滑和网状果皮横截面切片的木质素（lignin）（间苯三酚-盐酸染色）和木栓质（suberin）（荧光黄088）染色。

文献3: Gunawardena AH et al. Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae). Am J Bot. 2007 Jul;94(7):1116-28.

A）For fluorol yellow 088, a final concentration of 0.01% (w/v) was made by dissolving 0.01 g of fluorol yellow 088 in 50 mL of PEG 400 solution and heating at 90°C for 1 h (Brundrett et al., 1991). An equal volume of 90% (v/v) glycerol solution was added to the PEG staining solution. Leaves were stained for 1 h at room temperature, rinsed several times with distilled water, and observed with UV light for a bright yellow fluorescence. Unstained cell walls were also examined for autofluorescence as a test for the phenolic constituents of suberin.

图3：成熟花边植物叶的细胞壁组织化学检测。荧光黄088染脂肪族物质「A-D」。透化叶子（A，B）；新鲜叶子（C，D）。微分干涉相衬显微成像图片观察到棕色色素，而荧光成像图片观察到阳性染色（黄绿色荧光）。

注意事项

1）本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号

 名称 规格                

MS4024-25G       

 Aniline Blue (Water Soluble) 苯胺蓝（水溶） 25g
MS4027-5G Toluidine Blue O (TBO) 甲苯胺蓝O 

5g

MS4004A-25G

 Safranine O, BS Grade 番红O（生物染色级） 25g

MS4004B-25G

 Safranine O, High Purity Grade 番红O（高纯级）

25g
MS4074-1G Sudan Red 7B (Solvent Red 19) 苏丹红7B（溶剂红19）       

1g

MX4473-1G

 Fluorol Yellow 088 荧光黄088

1g

 

 

Fluorol Yellow 088 荧光黄088

发表于由

描述

Fluorol Yellow 088 荧光黄088

产品标签

Fluorol Yellow 088 荧光黄088；Solvent Green 4 溶绿4；Suberin lamellae 木栓质层；Ruthenium Red 钌红；Calcofluor White Stain 卡尔科弗卢尔荧光增白剂；CAS NO：81-37-8；

产品信息

产品名称

 产品编号 CAS NO.         规格             价格（元）        

Fluorol Yellow 088 荧光黄088       

 MX4473-1G      81-37-8 1g 999
Fluorol Yellow 088 荧光黄088 MX4473-5G 81-37-8 5g

3999
Fluorol Yellow 088 荧光黄088 MX4473-25G 81-37-8 25g

14299

产品描述

荧光黄088（Fluorol Yellow 088）是一种多环芳烃有机染料，一种亲脂性荧光素，用于植物组织木栓质层的细胞染色。

​

产品特性

英文同义名：2,8-Dimethylnaphtho[3,2,1-kl]xanthene; Solvent Green 4; C.I.Fluorescent Brightener 74; Fluorescent Green LF; Fluorol 5G;
中文同义名：2,8-二甲基-萘并[3,2,1-KL]占吨；溶绿4； CAS NO：81-37-8
分子式：C22H16O 分子量：296.36g/mol

纯度：≥95%

荧光：λex 447-453 nm (in methanol)
溶解性：部分溶于水，溶于DMSO，DMF，氯仿和乙酸乙酯 外观：黄色至橙色固体

保存与运输方法

保存：室温避光干燥保存，2年有效。

运输：室温运输。

应用实例

 

文献1:Lux A, Morita S, Abe J, Ito K, 2005.An improved method for clearing and staining free-hand sections and whole-mount samples. Ann Bot (Lond) 96:989–996

A）Clearing solution：Lactic acid was saturated with chloral hydrate.

B）(a) A 0.1 % (w/v) solution of berberine hemisulphate (小檗碱) in lactic acid was dissolved at room temperature. (b) A 0.01 % (w/v) solution of fluorol yellow 088 (荧光黄088, MX4473) in lactic acid was prepared by heating at 70°C for 1h. Due to the low stability of the solution, this was prepared fresh before each use.

C) Clearing and staining procedure

The sections floating in drops of clearing solution on microscope slides were heated over a water bath in covered Petri dishes. Treatment for 1h, using a high temperature (70°C), even with large or dense sections, was usually sufficient. The clearing solution was then absorbed by pipette or tissue paper and the sections were thoroughly washed with distilled water added several times to the sections and absorbed by tissue paper. Sections were post-stained and subsequently washed in the same way. Framing by a liquid blocker (PAP pen) proved useful to prevent the loss of sections from slides.

 

For observation of cell files along the root axis,whole roots were cleared and stained in lactic acid with fluorescence stains (berberine or fluorol yellow) and post-stained with safranin O.This procedure allows observation of epidermal and exodermal cells in thick roots of various species, and in thin roots of arabidopsis it works well for staining and observation of endodermal cells. In thick roots such as those of maize or sorghum, peeling peripheral root tissues exposes the endodermal cells and allows their direct observation. The contrast of cell walls is increased after treatment of peeled samples with berberine in lactic acid.

图1.「D-H」甜瓜不定根的切片经透明处理和含荧光黄088的乳酸染色液染色，在白光「D」、紫外光「E」和两种光下的重叠图片「F」。

文献2: Cohen H et al. A Multilevel Study of Melon Fruit Reticulation Provides Insight into Skin Ligno-Suberization Hallmarks. Plant Physiol. 2019 Apr;179(4):1486-1501.

A）Histochemical observations of suberin and lignin in skin cross sections were achieved by staining with a freshly prepared solution of Fluorol Yellow 088 (0.01% [w/v] in lactic acid) for 30 min at 70°C and phloroglucinol-hydrochloric acid (2% [w/v] in 50% hydrochloric acid) for 30 min at room temperature, respectively. Stained sections were then observed with an Olympus CLSM500 microscope using bright-field and GFP filters. Autofluorescence of cuticle layers in smooth skin sections was observed with a Cy5 filter.

图2.光滑和网状果皮横截面切片的木质素（lignin）（间苯三酚-盐酸染色）和木栓质（suberin）（荧光黄088）染色。

文献3: Gunawardena AH et al. Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae). Am J Bot. 2007 Jul;94(7):1116-28.

A）For fluorol yellow 088, a final concentration of 0.01% (w/v) was made by dissolving 0.01 g of fluorol yellow 088 in 50 mL of PEG 400 solution and heating at 90°C for 1 h (Brundrett et al., 1991). An equal volume of 90% (v/v) glycerol solution was added to the PEG staining solution. Leaves were stained for 1 h at room temperature, rinsed several times with distilled water, and observed with UV light for a bright yellow fluorescence. Unstained cell walls were also examined for autofluorescence as a test for the phenolic constituents of suberin.

图3：成熟花边植物叶的细胞壁组织化学检测。荧光黄088染脂肪族物质「A-D」。透化叶子（A，B）；新鲜叶子（C，D）。微分干涉相衬显微成像图片观察到棕色色素，而荧光成像图片观察到阳性染色（黄绿色荧光）。

注意事项

1）本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号

 名称 规格                

MS4024-25G       

 Aniline Blue (Water Soluble) 苯胺蓝（水溶） 25g
MS4027-5G Toluidine Blue O (TBO) 甲苯胺蓝O 

5g

MS4004A-25G

 Safranine O, BS Grade 番红O（生物染色级） 25g

MS4004B-25G

 Safranine O, High Purity Grade 番红O（高纯级）

25g
MS4074-1G Sudan Red 7B (Solvent Red 19) 苏丹红7B（溶剂红19）       

1g

MX4473-1G

 Fluorol Yellow 088 荧光黄088

1g

 

 

Lucifer Yellow Cadaverine，149733-79-9

发表于由

Lucifer Yellow Cadaverine 通常作为可固定的荧光示踪剂。Lucifer Yellow Cadaverine 可以标记细胞中的内吞噬泡。由于其水溶解度高、荧光明亮、细胞毒性低，并且具有较宽的斯托克斯位移（Stokes shift），我们可以在固定和活细胞中观察内吞噬泡。此外，它还可以用于结合羧酸基团。

产品名称 Lucifer Yellow Cadaverine，149733-79-9
目录号 910823
中文名称 萤光黄尸胺
英文名称 Lucifer Yellow Cadaverine
CAS 149733-79-9
分子式 C17H17K2N3O8S2
分子量 534
存储条件 -20°干燥避光
Ex/Em(nm) 428/536