VB27(+),细胞培养添加剂27,VISTECH 现货供应

Vistech Cell Culture Supplement(RA+)(50×)

Cat. No.: VB27(+)

Storage: -5°C to -20°C in dark

Package: 10ml

Shelf life: 12 months

 

Product description:

Vistech CCS27 Supplement(RA+) is an optimized serum-free supplement used to support the growth and viability of embryonic, post-natal, adult, hippocampal and other central nervous system (CNS) neurons. CCS27 Supplement(RA+) is provided as a 50X liquid and is intended to be used with basic medium for cell culture of nearly pure populations of neuronal cells without the need for an astrocyte feeder layer.

Vistech CCS27补剂(RA+)是一种优化的无血清补剂,用于支持胚胎、出生后、成人、海马和其他中枢神经系统(CNS)神经元的生长和活力。CCS27补充剂(RA+)为50X液体,旨在与基本培养基一起用于几乎纯的神经细胞群体的细胞培养,而不需要星形胶质细胞喂养层。

Applications:

CCS27 Supplement(RA+) when used as a supplement to DMEM/F-12 has been demonstrated to support the expansion of EGF-responsive precursor cells from rat embryonic striatum and mesencephalon.

CCS27 Supplement(RA+) when used as a supplement to Neurobasal Medium is effective for the culturing of tumor cell lines of neuronal origin and optimal viability and long-term survival of pre-natal and embryonic neuronal cells..

CCS27 Supplement(RA+) when used as a supplement to Neurobasal-A Medium is effective for optimal viability and long-term survival of post-natal and adult brain neuronal cells.

CCS27补充剂(RA+)作为DMEM/F-12的补充已被证明可支持大鼠胚胎纹状体和中脑中egf反应性前体细胞的扩增。

CCS27补充剂(RA+)作为神经基础培养基的补充,对神经来源的肿瘤细胞系的培养有效,并能使产前和胚胎神经细胞获得最佳的活力和长期存活。

CCS27补充剂(RA+)作为neurobase – a培养基的补充,对产后和成年脑神经细胞的最佳活力和长期存活有效。

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CCS27 Supplement(RA-), VB27(-),VISTECH 血清代理

Vistech Cell Culture Supplement(RA-)(50×)

Cat. No.: VB27(-)

Storage: -5°C to -20°C in dark

Package: 10ml

Shelf life: 12 months

 

Product description:

Vistech CCS27 Supplement(RA-) is is a serum-free, customized Cell Culture Supplement without Vitamin A (retinyl acetate). The absence of vitamin A makes this formulation ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. Vistech CCS27 Supplement(RA-) is provided as a 50× liquid.

Vistech CCS27补充剂(RA-)是一种无血清、不含维生素a(醋酸视黄酯)的定制细胞培养补充剂。缺乏维生素A使得该配方非常适合神经祖细胞和干细胞的培养,无论是作为悬浮的神经球还是在粘附的单层培养中,都不会诱导分化。Vistech CCS27补充剂(RA-)为50x液体。

Applications:

CCS27 Supplement(RA-) when used as a supplement to Neurobasal(pre-natal/embryonic cells), Neurobasal(post-natal/adult) or DMEM/F12 supports the serum-free growth of Central Nervous System (CNS) and Peripheral Nervous System (PNS) embryonic and adult neural stem cells (NSCs) or neural precursor cells. Epidermal Growth Factor (EGF) and basic-Fibroblast Growth Factor (bFGF) is necessary to prevent spontaneous differentiation of NSCs in culture.

CCS27补充剂(RA-)作为Neurobasal(产前/胚胎细胞)、Neurobasal(产后/成体)或DMEM/F12的补充,支持中枢神经系统(CNS)和周围神经系统(PNS)胚胎和成体神经干细胞(NSCs)或神经前体细胞的无血清生长。表皮生长因子(EGF)和碱性成纤维细胞生长因子(bFGF)是防止NSCs在培养过程中自发分化所必需的。

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mLIF蛋白质, VF-mLIF,VISTECH 血清代理

Catalog Number:

mLIF Protein

VF-mLIF

Description:

VISTECH® Recombinant Mouse LIF Protein (Vistech mLIF)

Packaging Information:

1.0 ml (10000×)

Working Concentration:

0.1 μL mLIF/ 1 mL culture medium

Background Information:

Mouse Leukemia Inhibitory Factor (mLIF) promotes proliferation and maintains long-term pluripotency of mouse embryonic stem (mES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.

小鼠白血病抑制因子(mLIF)通过抑制小鼠胚胎干细胞(mES)和诱导多能干细胞(iPS)的自发分化,促进细胞增殖并维持其长期多能性。

Applications:

VISTECH® mLIF is supplied in liquid form as 10000× in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability. Vistech mLIF is used as a reagent for the in vitro maintenance of the pluripotential phenotype of mouse ES cells and induced pluripotent stem (iPS) cells. The working concentration is recommended at 0.1 μL mLIF per 1 mL culture medium. So each package of VF-LIF103U can make up 10 L culture medium of mES and iPS.

VISTECH®mLIF以液体形式提供10000x,在1.0 mL磷酸盐缓冲盐水中,以1% w/v牛血清白蛋白BSA作为稳定性载体。Vistech mLIF被用作体外维持小鼠胚胎干细胞和诱导多能干细胞(iPS)多能表型的试剂。推荐工作浓度为每1ml培养基0.1 μL mLIF。因此,每包VF-LIF103U可以组成10 L的mES和iPS培养基。

Storage Conditions:

Shipped at ambient temperature

Storage at 4°C for long term

Don’t freeze-thawing

Stability for at least 18 months from the date of manufacture (in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells)

Stability for at least 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

USE RESTRICTED TO RESEARCH USE ONLY

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