标签归档：Vector Labs

Vectorlabs代理-Fluorescein Maleimide, 荧光素马来酰亚胺12 mg

发表于由

Vectorlabs代理-Fluorescein Maleimide, 荧光素马来酰亚胺12 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Fluorescein Maleimide, 12 mg

品牌：Vectorlabs
产品货号：SP-1502-12
规格：12 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准

  • 产品名称:Fluorescein Maleimide, 12 mg

    别名:荧光素标记的马来酰亚胺

    产品描述:

    Fluorescein Maleimide is designed for fluoresceinating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. In the case of proteins, Fluorescein Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag®, 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents. (Excitation maximum at 495 nm; emission maximum at 515 nm).

Vectorlabs代理-Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

发表于由

Vectorlabs代理-Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

品牌：Vectorlabs

产品货号：AL-1023S-5

规格：5 ml

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

别名:琥珀酰化小麦胚芽凝集素，琼脂糖交联

产品描述:

Wheat germ agglutinin (WGA) succinylated has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.Agarose bound*, succinylated WGA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: Chitin Hydrolysate (Cat. No. SP-0090) or 500 mM N-acetylglucosamine with salt and/or acid elution generally required*3 mg lectin/ml gel

据报道，琥珀酰化的小麦胚芽凝集素 (WGA) 具有不同于天然凝集素的特性。有证据表明，与天然形式不同，琥珀酰化小麦胚芽凝集素不与唾液酸残基结合，但保留了其对 N-乙酰氨基葡萄糖的特异性。使用天然凝集素和琥珀酰化形式的缀合物可以提供一个系统来区分唾液酸化糖缀合物和仅含有 N-乙酰氨基葡萄糖结构的糖缀合物。琼脂糖结合*，琥珀酰化 WGA 是使用我们的亲和纯化凝集素制备的。具有约 2×107 道尔顿分子量排除极限的热稳定、交联的 4% 琼脂糖珠用作凝集素共价偶联的固相基质。凝集素与珠子的连接受到仔细控制，以保持凝集素活性并最大限度地减少可能导致非特异性离子或疏水相互作用的结合凝集素的构象变化。我们开发的将凝集素与琼脂糖珠偶联的技术在凝集素和基质之间插入了一个亲水性间隔臂。这种偶联方法与传统的溴化氰方法相比具有几个优点：保持偶联凝集素的最大碳水化合物结合活性pH 值范围 结合的蛋白质不会被 Tris 或其他常规使用的缓冲液从珠子上浸出 结合后不存在残留电荷。这最大限度地减少了与基质的非特异性结合。我们的琼脂糖结合凝集素以每毫升沉降珠子的恒定凝集素浓度提供。选择每种凝集素的浓度以实现珠中每毫克凝集素的最高糖缀合物结合能力。使用已知结合凝集素的糖蛋白测试每批的结合能力。这为用户提供了指导并确保了我们琼脂糖结合凝集素的质量。抑制/洗脱糖：甲壳素水解物（货号 SP-0090）或 500 mM N-乙酰氨基葡萄糖，通常需要盐和/或酸洗脱*3毫克凝集素/毫升凝胶

实验应用:Glycobiology,Affinity Chromatography

推荐稀释度:

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-5100. Alternatively, 0.5 M N-Acetyl-D-Glucosamine (GlcNAc) can be used.For those glycoconjugates having very high affinity for WGA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid and increase the concentration of GlcNAc. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.

使用前用缓冲液彻底清洗凝胶，以去除为稳定凝集素而添加的糖。 用于洗脱与该琼脂糖凝集素结合的糖缀合物的推荐产品：糖蛋白洗脱溶液，Cat. 编号 ES-5100。 或者，可以使用 0.5 M N-乙酰基-D-氨基葡萄糖 (GlcNAc)。对于那些对 WGA 具有非常高亲和力的糖缀合物，可能需要用乙酸将洗脱糖溶液的 pH 值降低到 3.0 并增加 GlcNAc 的浓度。 使用后，用几柱体积的缓冲盐水清洗凝胶，然后将凝胶重悬于含有 0.08% 叠氮化钠的缓冲盐水中保存。

偶联:Agarose

存储溶液:10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide

样本类型:10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide

Vectorlabs 抗体-Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg

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Vectorlabs 抗体-Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg

  • 产品名称:Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg
  • 别名:山羊抗人IgG (H+L)抗体，HRP标记
  • 产品描述:
    Goat Anti-Human IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.
  • 反应种属:Human
  • 宿主来源:Goat
  • 实验应用:Immunohistochemistry / Immunocytochemistry,Blotting Applications,Elispot,ELISAs
  • 推荐稀释度:
    Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti human IgG is 0.5-5.0µg/ml.
  • 偶联:Peroxidase
  • 产品浓度:1 mg active conjugate/ml
  • 产品形式:Concentrate
  • 存储溶液:10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 产地:美国
  • 样本类型:10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs 苏木精/苏木素复染试剂-Hematoxylin Counterstain, 500 ml

发表于由

Vectorlabs 苏木精/苏木素复染试剂-Hematoxylin Counterstain, 500 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Hematoxylin Counterstain, 500 ml

品牌：Vectorlabs
产品货号：H-3401-500
规格：500 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Hematoxylin Counterstain, 500 ml
  • 别名:苏木精/苏木素复染试剂
  • 产品描述:
    Vector’s Hematoxylin counterstain produces blue stained nuclei with crisp detail. This commonly utilized counterstain is based on Gill s formulation and is mercury free. As this is a progressive stain formula, the intensity of nuclear staining can be adjusted to achieve the most desirable results either manually or in an automated instrument. This counterstain provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates and is suitable for use with non-aqueous and aqueous mountants.
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 产品形式:
    Ready-to-Use
  • 产地:
    美国

Vectorlabs TEXAS RED标记的马来酰亚胺-Texas Red Maleimide, 3.6 mg

发表于由

Vectorlabs TEXAS RED标记的马来酰亚胺-Texas Red Maleimide, 3.6 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Texas Red® Maleimide, 3.6 mg

品牌：Vectorlabs
产品货号：SP-1505-3.6
规格：3.6 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:Texas Red Maleimide, 3.6 mg
  • 别名:TEXAS RED标记的马来酰亚胺
  • 产品描述:
    Texas Red Maleimide is designed for fluorescent labeling of proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Texas Red® Maleimide require free thiol groups. In the case of proteins, Texas Red Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag®, 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.This fluorescent label is a high quantum yield rhodamine derivative that can be directly visualized. The label can also be detected, and the signal amplified, using our affinity-purified antibody conjugates to rhodamine. (Excitation maximum at 595 nm; emission maximum at 615 nm).
  • 预期分子量:728
  • 产地:美国

Vectorlabs 花生凝集素，Cy3标记-Peanut Agglutinin (PNA), CY3, 1 mg

发表于由

Vectorlabs 花生凝集素，Cy3标记-Peanut Agglutinin (PNA), CY3, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Peanut Agglutinin (PNA), CY3, 1 mg

品牌：Vectorlabs
产品货号：CL-1073-1
规格：1 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准

产品名称:Peanut Agglutinin (PNA), CY®3, 1 mg

别名:花生凝集素，Cy3标记

产品描述:

Cy3 PEANUT AGGLUTININ PNA binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.Cy3 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.Excitation: 550 nmEmission: 570 nmColor: Red

实验应用:Immunofluorescence,Glycobiology

推荐稀释度:The recommended concentration range for use is 5-20µg/ml.

偶联:Cy3

产品浓度:1 mg active conjugate/ml

存储溶液:10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

产地:美国

样本类型:10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

Vectorlabs Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml

发表于由

Vectorlabs代理-Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml

品牌：Vectorlabs
产品货号：AL-1063-2
规格：2 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml
  • 别名:
    荆豆凝集素I，琼脂糖交联
  • 产品描述:
  • Ulex europaeus agglutinin I (UEA I) binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.Agarose bound* Ulex europaeus agglutinin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins. Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose*2 mg lectin/ml gel
  • 实验应用:
    Glycobiology,Affinity Chromatography
  • 推荐稀释度:
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 偶联:
    Agarose
  • 存储溶液:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide
  • 产地:
    美国
  • 样本类型:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide

Vectorlabs Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg

发表于由

Vectorlabs Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg

品牌：Vectorlabs
产品货号：PI-9400-1
规格：1 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg
  • 别名:
    山羊抗大鼠IgG（H+L）抗体，HRP标记
  • 产品描述:
    Goat Anti-Rat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.Peroxidase Goat Anti-Rat IgG is supplied in solution. With some exceptions, working concentration of HRP Anti-Rat IgG is 0.5-5.0 ug/ml.
  • 反应种属:
    Rat
  • 宿主来源:
    Goat
  • 实验应用:
  • Immunohistochemistry / Immunocytochemistry,Blotting Applications,Elispot,ELISAs
  • 推荐稀释度:
  • Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti rat IgG is 0.5-5.0µg/ml.
  • 偶联:
    Peroxidase
  • 产品浓度:
    1 mg active conjugate/ml
  • 产品形式:
    Concentrate
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs Cy5标记花生凝集素 Peanut Agglutinin (PNA), CY5, 1 mg

发表于由

Vectorlabs Cy5标记花生凝集素 Peanut Agglutinin (PNA), CY5, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。Peanut Agglutinin (PNA), CY®5, 1 mg

品牌：Vectorlabs
产品货号：CL-1075-1
规格：1 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Peanut Agglutinin (PNA), CY®5, 1 mg
  • 别名:
    花生凝集素，Cy5标记
  • 产品描述:
  • CY5 PEANUT AGGLUTININ PNA binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.Cy5 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.Excitation: 649 nmEmission: 670 nmColor: Far Red
  • 实验应用:
    Immunofluorescence,Glycobiology
  • 推荐稀释度:
    The recommended concentration range for use is 5-20µg/ml.
  • 偶联:
    Cy5
  • 产品浓度:
    1 mg active conjugate/ml
  • 存储溶液:
  • 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
  • 样本类型:
  • 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

Vectorlabs 甲基绿复染试剂 Methyl Green Counterstain, 500 ml

发表于由

Vectorlabs 甲基绿复染试剂 Methyl Green Counterstain, 500 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Methyl Green Counterstain, 500 ml

品牌：Vectorlabs
产品货号：H-3402-500
规格：500 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Methyl Green Counterstain, 500 ml
  • 别名:
    甲基绿复染试剂
  • 产品描述:
  • Vector’s Methyl Green counterstain cell nuclei light green. This superior formulation of methyl green is suitable for use with a wide range of enzyme substrates. Optimal tissue staining is achieved by using a simple, two-step procedure. This counterstain provides an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors. This counterstain is suitable for use with non-aqueous mountants.
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 产品形式:
    Ready-to-Use

Vectorlabs代理-VECTABOND® Reagent, Tissue Section Adhesion, 7 ml

发表于由

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。VECTABOND® Reagent, Tissue Section Adhesion, 7 ml

品牌： Vectorlabs
产品货号： SP-1800-7
规格： 7 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准

  • 产品名称:
    VECTABOND® Reagent, Tissue Section Adhesion, 7 ml
  • 别名:
    组织切片粘附剂
  • 产品描述:
    VECTABOND Reagent is designed to significantly increase the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips. Tissue sections will remain attached even when subjected to the most extreme conditions, such as high temperature antigen unmasking techniques and in situ hybridization. VECTABOND™ Reagent treated slides can be stored for long periods. This product chemically modifies the glass to form a highly adherent surface. VECTABOND™ Reagent is provided as 7 ml of concentrate that dilutes to 350 ml of treatment solution, sufficient for at least 500 standard slides.
  • 反应种属:
  • 宿主来源:
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,Immunofluorescence,In situ hybridization
  • 靶标/特异性:
  • 同种型:
  • 推荐稀释度:
    The VECTABOND® Reagent for Tissue Section Adhesion is designed to significantly increase the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips. It chemically modifies the surface of the glass with positive charges to form a highly adherent surface.
  • 免疫原:
  • 免疫原种属:
  • 克隆性:
  • 克隆号:
  • 纯化方式:
  • 偶联:
  • 产品浓度:
  • 表达系统:
  • 阳性对照:
  • 保存温度:
  • 运输温度:
  • 细胞定位:
  • 预期分子量:
  • 产品形式:
  • 存储溶液:
  • 有效期:
    效期最长8~10个月
  • 产地:
    美国
  • 外观:
  • 生产商:
  • 功能与背景:
  • URL:
  • 灵敏度:
  • 检测范围:
  • 检测方法:
  • 样本类型:
  • 产品特点:
  • 纯度:
  • 生物活性:
  • 内毒素水平:
  • 来源:
  • 表达方式:
  • Accession #:
  • CAS号:
  • 分子式:
  • 溶解度:
  • PubChem CID:

Vectorlabs代理-Peanut Agglutinin (PNA), Agarose bound, 2 ml

发表于由

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。Peanut Agglutinin (PNA), Agarose bound, 2 ml

品牌： Vectorlabs
产品货号： AL-1073-2
规格： 2 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准

  • 产品名称:
    Peanut Agglutinin (PNA), Agarose bound, 2 ml
  • 别名:
    花生凝集素，琼脂糖交联
  • 产品描述:
    Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.Agarose bound* PNA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 200 mM galactose*5 mg lectin/ml gel
  • 反应种属:
  • 宿主来源:
  • 实验应用:
    Glycobiology,Affinity Chromatography
  • 靶标/特异性:
  • 同种型:
  • 推荐稀释度:
    Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Use of buffers containing 0.1 mM CaCl2 and 0.01 mM MnCl2 is recommended. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 200 mM galactose in 10 mM HEPES-buffered saline, pH 7.5 can be used.For those glycoconjugates having very high affinity for PNA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 免疫原:
  • 免疫原种属:
  • 克隆性:
  • 克隆号:
  • 纯化方式:
  • 偶联:
    Agarose
  • 产品浓度:
  • 表达系统:
  • 阳性对照:
  • 保存温度:
  • 运输温度:
  • 细胞定位:
  • 预期分子量:
  • 产品形式:
  • 存储溶液:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide
  • 有效期:
  • 产地:
    美国
  • 外观:
  • 生产商:
  • 功能与背景:
  • URL:
  • 灵敏度:
  • 检测范围:
  • 检测方法:
  • 样本类型:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide
  • 产品特点:
  • 纯度:
  • 生物活性:
  • 内毒素水平:
  • 来源:
  • 表达方式:
  • Accession #:
  • CAS号:
  • 分子式:
  • 溶解度:
  • PubChem CID:

Vectorlabs 山羊抗大鼠IgG-Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg

发表于由

Vectorlabs 山羊抗大鼠IgG-Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg

品牌：Vectorlabs
产品货号：PI-9401-0.5
规格：0.5 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
  • Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg
  • 别名:
    山羊抗大鼠IgG（H+L）抗体，HRP标记
  • 产品描述:
  • Goat Anti-Rat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The anti-rat IgG (H+L), mouse adsorbed products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross-react with mouse immunoglobulins. This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues.Peroxidase Goat Anti-Rat IgG, mouse adsorbed, is supplied in solution. With some exceptions, working concentration of HRP Anti-Rat IgG, mouse adsorbed, is 0.5-5.0 ug/ml.
  • Goat Anti-Rat IgG Antibody 通过亲和层析纯化并吸附以去除任何可能干扰特异性标记的交叉反应。然后该抗体与最高比活性的辣根过氧化物酶交联。专有的偶联程序可最大限度地保留酶活性和抗体特异性。该抗体经过严格的质量控制检测，可用于组织染色、ELISA 和印迹。这种特殊制备的抗体设计用于可能含有干扰性内源性免疫球蛋白的组织。抗大鼠 IgG (H+L)、小鼠吸附产品已通过固相技术彻底吸附，以去除与小鼠免疫球蛋白交叉反应的抗体。这种吸附的抗体允许在大鼠中制备的一抗用于小鼠组织。过氧化物酶山羊抗大鼠 IgG，小鼠吸附，以溶液形式提供。除了一些例外，小鼠吸附的 HRP Anti-Rat IgG 的工作浓度为 0.5-5.0 ug/ml。
  • 反应种属:
    Rat
  • 宿主来源:
    Goat
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,Blotting Applications,Elispot,ELISAs
  • 推荐稀释度:
  • Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti rat IgG is 0.5-5.0µg/ml.
  • 推荐用于微量滴定板测定的稀释剂：10 mM 磷酸盐，0.15 M NaCl，pH 7.5，0.1% Tween 20。HRP-抗大鼠 IgG 的工作浓度为 0.5-5.0µg/ml。
  • 产品形式:
    Concentrate
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs Cy3标记接骨木凝集素 Sambucus Nigra Lectin (SNA, EBL), CY3, 1 mg

发表于由

Vectorlabs Cy3标记接骨木凝集素 Sambucus Nigra Lectin (SNA, EBL), CY3, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Sambucus Nigra Lectin (SNA, EBL), CY®3, 1 mg

品牌：Vectorlabs
产品货号：CL-1303-1
规格：1 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Sambucus Nigra Lectin (SNA, EBL), CY®3, 1 mg
  • 别名:
    接骨木凝集素，Cy3标记
  • 产品描述:
  • Cy3 Sambucus nigra lectin, SNL isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.Cy3 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. Excitation: 550 nmEmission: 570 nmColor: Red
  • Cy3 Sambucus nigra 凝集素，从接骨木树皮中分离出来的 SNL，优先与 α-2,6 末端半乳糖连接的唾液酸结合，而α-2,3 键的结合程度较低。 乳糖或半乳糖也在一定程度上抑制了结合。 这种凝集素似乎不结合与 N-乙酰半乳糖胺相连的唾液酸。 据报道，SNA 可抑制无细胞蛋白质合成。Cy3 标记的黑接骨木凝集素具有适当数量的荧光染料，可以为这种凝集素提供最佳染色特性。 该偶联物基本上不含未偶联的荧光染料。 激发：550 纳米发射：570 纳米颜色：红色
  • 实验应用:
    Immunofluorescence,Glycobiology
  • 推荐稀释度:
    The recommended concentration range for use is 5-20µg/ml.
  • 偶联:
    Cy3
  • 产品浓度:
    1 mg active conjugate/ml
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

Vectorlabs 亲和素/生物素封闭试剂盒 Avidin/Biotin Blocking Kit

发表于由

Vectorlabs 亲和素/生物素封闭试剂盒 Avidin/Biotin Blocking Kit

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Avidin/Biotin Blocking Kit, 1 Kit

品牌：Vectorlabs
产品货号：SP-2001
规格：1 Kit
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Avidin/Biotin Blocking Kit, 1 Kit
  • 别名:
    亲和素/生物素封闭试剂盒
  • 产品描述:
  • Avidin Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and avidin binding sites present in tissues. This kit is designed for use with avidin detection systems such as the VECTASTAIN® ABC Kits if avidin or biotinylated reagents bind non-specifically to tissues or proteins.This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles.
  • Avidin Biotin Blocking Kit 可阻断组织中存在的所有内源性生物素、生物素受体和亲和素结合位点。 如果抗生物素蛋白或生物素化试剂与组织或蛋白质非特异性结合，则该试剂盒设计用于抗生物素蛋白检测系统，例如 VECTASTAIN® ABC 试剂盒。该封闭试剂盒由 18 毫升 Avidin D 和 18 毫升生物素组成，装在方便的滴管瓶中 .
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,Immunofluorescence,In situ hybridization,Blotting Applications,Elispot

Vectorlabs 核固红复染试剂 Nuclear Fast Red Counterstain, 500 ml

发表于由

Vectorlabs 核固红复染试剂 Nuclear Fast Red Counterstain, 500 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Nuclear Fast Red Counterstain, 500 ml

品牌：Vectorlabs
产品货号：H-3403-500
规格：500 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Nuclear Fast Red Counterstain, 500 ml
  • 别名:
    核固红复染试剂
  • 产品描述:
  • Vector’s Nuclear Fast Red counterstain stained nuclei have a pink appearance. This counterstain provides good contrast with a variety of substrates and is suitable for use with nonaqueous mountants. The staining procedure is rapid requiring only one step. This counterstain provides an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 产品形式:
    Ready-to-Use
  • 有效期:
    效期最长8个月

Vectorlabs 马抗山羊IgG Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

发表于由

Vectorlabs 马抗山羊IgG Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

品牌：Vectorlabs
产品货号：PI-9500-1
规格：1 mg
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg
  • 别名:
    马抗山羊IgG（H+L）抗体，HRP标记
  • 产品描述:
  • Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.As well as recognising goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.
  • Horse Anti-Goat IgG Antibody 通过亲和层析纯化并吸附以去除任何可能干扰特定标记的交叉反应性。 然后该抗体与最高比活性的辣根过氧化物酶交联。 专有的偶联程序可最大限度地保留酶活性和抗体特异性。 该抗体经过严格的质量控制检测，可用于组织染色、ELISA 和印迹。除识别山羊 IgG 外，该抗体还适用于检测绵羊和牛 IgG 一抗。
  • 反应种属:
    Goat
  • 宿主来源:
    Horse
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization,Blotting Applications,Elispot,ELISAs
  • 推荐稀释度:
  • Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in thse applications, blocking with Animal-Free blocker‚Ñ¢ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti goat IgG is 0.5-5.0µg/ml.
  • 重要提示：在 ELISA 或印迹分析中使用牛产品（如酪蛋白、血清、白蛋白或脱脂奶粉）作为封闭剂可能会产生高背景，因为抗山羊 IgG 与牛免疫球蛋白可能会产生交叉反应。 如果要在这些应用中使用该抗体，建议使用无动物阻滞剂，Ñ¢ 溶液（货号 SP-5030）进行封闭。 推荐用于微量滴定板测定的稀释剂：10 mM 磷酸盐、0.15 M NaCl、pH 7.5、0.1% Tween 20。HRP-抗山羊 IgG 的工作浓度为 0.5-5.0µg/ml。
  • 偶联:
    Peroxidase
  • 产品浓度:
    1 mg active conjugate/ml
  • 产品形式:
    Concentrate
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs 琼脂糖交联花生凝集素 Peanut Agglutinin (PNA), Agarose bound, 5 ml

发表于由

Vectorlabs 琼脂糖交联花生凝集素 Peanut Agglutinin (PNA), Agarose bound, 5 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Peanut Agglutinin (PNA), Agarose bound, 5 ml

品牌：Vectorlabs
产品货号：AL-1073-5
规格：5 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Peanut Agglutinin (PNA), Agarose bound, 5 ml
  • 别名:
    花生凝集素，琼脂糖交联
  • 产品描述:
  • Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.Agarose bound* PNA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 200 mM galactose*5 mg lectin/ml gel
  • 花生凝集素 (PNA) 优先与 T 抗原结合，这是一种半乳糖基 (β-1,3) N-乙酰半乳糖胺结构，存在于许多糖缀合物中，例如 M 和 N 血型、神经节苷脂和许多其他可溶性和膜相关糖蛋白和糖脂。除了某些例外，PNA 的受体序列通常是唾液酸化的，这会阻止凝集素与其受体寡糖结合（参见 Jacalin）。甚至不直接与受体糖结合的唾液酸也可能抑制结合。稀释剂中钙离子的存在可以增强 PNA 与受体的结合，可能通过中和受体序列附近唾液酸残基上的负电荷。PNA 可用于区分正常组织和肿瘤组织以及评估移行黏膜的恶性肿瘤。此外，PNA 结合可用于测量淋巴组织中的细胞成熟度，区分人和实验动物中的各种淋巴细胞亚群，以及测量许多疾病中淋巴细胞群的水平。 PNA 可用于分离小鼠干细胞，用于跨越组织相容性障碍的骨髓移植。PNA 的主要细胞表面受体可能是去唾液酸 GM1 神经节苷脂。由于 PNA 与针对这种糖脂的抗体具有特异性，因此 PNA 和抗体可以在某些应用中互换使用。琼脂糖结合* PNA 是使用我们的亲和纯化凝集素制备的。具有约 2×107 道尔顿分子量排除极限的热稳定、交联的 4% 琼脂糖珠用作凝集素共价偶联的固相基质。凝集素与珠子的连接受到仔细控制，以保持凝集素活性并最大限度地减少可能导致非特异性离子或疏水相互作用的结合凝集素的构象变化。我们开发的将凝集素与琼脂糖珠偶联的技术在凝集素和基质之间插入了一个亲水性间隔臂。这种偶联方法与传统的溴化氰方法相比具有几个优点：保持偶联凝集素的最大碳水化合物结合活性pH 值范围 结合的蛋白质不会被 Tris 或其他常规使用的缓冲液从珠子上浸出 结合后不存在残留电荷。这最大限度地减少了与基质的非特异性结合。我们的琼脂糖结合凝集素以每毫升沉降珠子的恒定凝集素浓度提供。选择每种凝集素的浓度以实现珠中每毫克凝集素的最高糖缀合物结合能力。使用已知结合凝集素的糖蛋白测试每批的结合能力。这为用户提供了指导并确保了我们琼脂糖结合凝集素的质量。抑制/洗脱糖：200 mM 半乳糖*5 mg 凝集素/ml 凝胶
  • 实验应用:
    Glycobiology,Affinity Chromatography
  • 推荐稀释度:
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Use of buffers containing 0.1 mM CaCl2 and 0.01 mM MnCl2 is recommended. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 200 mM galactose in 10 mM HEPES-buffered saline, pH 7.5 can be used.For those glycoconjugates having very high affinity for PNA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 使用前用缓冲液彻底清洗凝胶，以去除为稳定凝集素而添加的糖。 建议使用含有 0.1 mM CaCl2 和 0.01 mM MnCl2 的缓冲液。 用于洗脱与这种琼脂糖凝集素结合的糖缀合物的推荐产品：糖蛋白洗脱溶液，目录 编号 ES-2100。 或者，可以使用 10 mM HEPES 缓冲盐水中的 200 mM 半乳糖，pH 7.5。对于那些对 PNA 具有非常高亲和力的糖缀合物，可能需要用乙酸将洗脱糖溶液的 pH 值降低到 3.0。 使用后，用几柱体积的缓冲盐水清洗凝胶，然后将凝胶重悬于含有 0.08% 叠氮化钠的缓冲盐水中保存。
  • 存储溶液:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2,20 mM galactose, 0.08% sodium azide
  • 样本类型:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2,20 mM
  • galactose, 0.08% sodium azide

Vectorlabs 链霉亲和素/生物素封闭试剂盒 Streptavidin/Biotin Blocking Kit

发表于由

Vectorlabs 链霉亲和素/生物素封闭试剂盒 Streptavidin/Biotin Blocking Kit

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Streptavidin/Biotin Blocking Kit, 1 kit

品牌：Vectorlabs
产品货号：SP-2002
规格：1 kit
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Streptavidin/Biotin Blocking Kit, 1 kit
  • 别名:
    链霉亲和素/生物素封闭试剂盒
  • 产品描述:
  • Streptavidin Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and streptavidin binding sites present in tissues. This kit is designed for use with streptavidin detection systems such as the VECTASTAIN® Universal Quick kits if streptavidin or biotinylated reagents bind non-specifically to tissues or proteins.This blocking kit consists of 18 ml of streptavidin and 18 ml of biotin in convenient dropper bottles.
  • Streptavidin Biotin Blocking Kit 可阻断组织中存在的所有内源性生物素、生物素受体和链霉亲和素结合位点。 如果链霉亲和素或生物素化试剂与组织或蛋白质非特异性结合，则该试剂盒设计用于链霉亲和素检测系统，例如 VECTASTAIN® Universal Quick 试剂盒。该封闭试剂盒由 18 毫升链霉亲和素和 18 毫升生物素组成，装在方便的滴管瓶中 .
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,Immunofluorescence,In situ hybridization,Blotting Applications,Elispot
  • 产品形式:
    Ready-to-Use

Vectorlabs 苏木精/苏木素QS复染试剂 Hematoxylin QS Counterstain, 100 ml

发表于由

Vectorlabs 苏木精/苏木素QS复染试剂 Hematoxylin QS Counterstain, 100 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌产品，欢迎访问Vector Labs官网或者咨询我们获取相关产品信息。
Vector Laboratories主要产品类别
ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Hematoxylin QS Counterstain, 100 ml

品牌：Vectorlabs
产品货号：H-3404-100
规格：100 ml
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Hematoxylin QS Counterstain, 100 ml
  • 别名:
    苏木精/苏木素QS复染试剂
  • 产品描述:
  • Vector’s Hematoxylin QS counterstain, a modification of Mayer s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-touse without filtration, requires no separate blueing step, and stains in less than 45 seconds. Vector Hematoxylin QS contains no mercury and provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates. This counterstain is suitable for use with nonaqueous and aqueous mountants.
  • Vector 的苏木精 QS 复染剂是 Mayer 苏木精的改良版，专为免疫细胞化学开发，可提供清晰的蓝色核染色。 该配方无需过滤即可使用，无需单独的上蓝步骤，并且可在 45 秒内染色。 Vector Hematoxylin QS 不含汞，与最常用的过氧化物酶和碱性磷酸酶底物具有出色的颜色对比。 这种复染剂适用于非水性和水性封固剂。
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 产品形式:
    Ready-to-Use