描述
Tunicamycin 衣霉素(N-连接糖基化抑制剂)
— — 糖蛋白合成和细胞周期研究核苷抗生素
搜索关键词:
Tunicamycin 衣霉素;链霉菌属 Streptomyces lysosuperficus;GlcNAc-PT, GPT活性抑制;N-连接糖基化抑制;竞争性细胞周期抑制剂;CAS:11089-65-9
产品订购:
货号 |
产品名称 |
规格 |
价格(元) |
MS0013-1MG |
Tunicamycin from Streptomyces lysosuperficus 衣霉素,来源于链霉菌属 |
1mg |
470 |
MS0013-5MG |
5mg |
1470 |
|
MS0013-10MG |
10mg |
2170 |
基本介绍:
衣霉素(Tunicamycin,CAS:11089-65-9),分离自链霉菌属(Streptomyces lysosuperficus)的代谢产物,由四种异构体A,B,C,D组成的混合物,普遍用于各种生物体系内的糖蛋白合成研究。衣霉素是一种核苷抗生素,体外可有效抵制革兰氏阳性菌(G+),真菌,酵母菌和病毒的生长与繁殖。衣霉素能抑制N-乙酰基-1-磷酸转移酶(GlcNAc phosphotransferase, GPT)活性,以及防止糖蛋白合成中的N-糖苷连接形成。在多萜醇连接的糖蛋白合成中衣霉素抑制糖基转移到多萜醇上。衣霉素还能剂量依赖性的抑制DNA合成(此特性可能与糖蛋白结构改变有关),因而影响胸苷转运进入细胞。衣霉素能抑制细胞周期的S期,诱使细胞周期停滞在G1晚期。文献报道,衣霉素能阻止大鼠视网膜原代细胞的细胞周期进程,且以剂量依赖方式抑制鸡或小鼠成纤维细胞内脂质介导的蛋白糖基化。衣霉素能抑制蛋白质棕榈酰化,并且诱导自吞噬发生。
基本特性:
1)CAS NO:11089-65-9
2)分子式/分子量:衣霉素为四种异构体A,B,C,D的混合物,具体信息如下,
异构体A, n=8, C37H60N4O16, 816.90g/mol
异构体B, n=9, C38H62N4O16, 830.93g/mol
异构体C, n=10, C39H64N4O16, 844.95g/mol
异构体D, n=11, C40H66N4O16, 858.99g/mol
3)纯度:≥95%(异构体混合物)
4)外观:白色至浅黄色或黄褐色粉末
5)溶解性:溶于DMSO和DMF(20mg/ml)、碱溶液、热甲醇
6)化学结构式:
保存与运输方法
保存:2-8℃干燥保存,或置于-20℃长期保存。至少2年稳定。
运输:冰袋运输。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用方法【源自文献,仅作参考】
文献1,Wang H et al. Tunicamycin-induced Unfolded Protein Response in the Developing Mouse Brain. Toxicol Appl Pharmacol. 2015 Mar 15; 283(3): 157–167. PMID: 25620058
体内研究(动物模型): 动物模型(Animal Model):C57BL/6 mice 药物配制(Preparation):Tunicamycin was diluted in 150 mM dextrose at a concentration of 0.3 μg/μl. The concentration was selected based on previous studies of tunicamycin injection in adult animals. 实验方法(Assay):Tunicamycin was administered on postnatal day 4 (PD4), PD12 and PD25. The mice received two subcutaneous injections of tunicamycin at 3 μg/g each and the injections were two hours apart. Mice in the control group were injected with the same amount of dextrose without tunicamycin. |
文献2,Zode GS et al. Reduction of ER stress via a chemical chaperone prevents disease phenotypes in a mouse model of primary open angle glaucoma. J Clin Invest. 2011 Sep 1; 121(9): 3542–3553. PMID: 21821918
体外研究(细胞实验): 动物模型(Animal Model):Normal C57BL/6J mice 药物配制(Preparation):Tunicamycin was dissolved in DMSO. 实验方法(Assay):For anterior chamber injections, the animals were anesthetized with a mouse anesthesia cocktail (ketamine [73 mg/kg], and xylazine [1.8 mg/kg]). Adenoviral vectors (3 × 109 PFU/eye) or tunicamycin (0.03, 0.3, and 3 μg/eye) were injected in the anterior chamber in a volume of 2 μl into both eyes of each animal. Mice that developed eye abnormalities were excluded from further study. DMSO injections were used as vehicle control for tunicamycin. |
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