标签归档：tnf

Recombinant Human TNF-β/TNFSF1

发表于由

产品编号

JP531-10μg
Recombinant Human TNF-β/TNFSF1
10μg

669

JP531-100μg
Recombinant Human TNF-β/TNFSF1
100μg

6138

JP531-500μg
Recombinant Human TNF-β/TNFSF1
500μg

10830

JP531-1mg
Recombinant Human TNF-β/TNFSF1
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

4049

JP1374

E. coli

171aa

18.7kDa

－

About this protein

Name

Recombinant Human TNF-β/TNFSF1 (Recombinant Human Tumor Necrosis Factor-β/TNFSF1; rHuTNF-β/TNFSF1)；重组人肿瘤坏死因子β/TNFS1

Synonyms

LTA; Lymphotoxin alpha; tnfb; TNF-beta; TNFSF1; TNFSF1B; LT; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); TNFBlymphotoxin-alpha; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5pg/ml, corresponding to a specific activity of >2.0×108IU/mg in the presence of actinomycin D.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20.

Endotoxin

Less than 0.1EU/μg of rHuTNF-β/TNFSF1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNFbeta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1.

Amino Acid Sequence

LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L

包装清单:

产品编号

产品名称

包装

JP531-10μg

Recombinant Human TNF-β/TNFSF1

10μg

JP531-100μg

Recombinant Human TNF-β/TNFSF1

100μg

JP531-500μg

Recombinant Human TNF-β/TNFSF1

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human TNF-α/TNFSF2, Variant

发表于由

产品编号

JP527-10μg
Recombinant Human TNF-α/TNFSF2, Variant
10μg

669

JP527-100μg
Recombinant Human TNF-α/TNFSF2, Variant
100μg

2420

JP527-500μg
Recombinant Human TNF-α/TNFSF2, Variant
500μg

5418

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

7124

JP1375

E. coli

151aa

16.9kDa

－

About this protein

Name

Recombinant Human TNF-α/TNFSF2, Variant (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, Variant; rHuTNF-α/TNFSF2, Variant)；重组人肿瘤坏死因子α/TNFSF2变体

Synonyms

Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01ng/ml, corresponding to a specific activity of >1.0×108IU/mg in the presence of actinomycin D.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.

Endotoxin

Less than 1EU/μg of rHu TNF-α/TNFSF2, Variant as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

The clinical use of the potent antitumor activity of TNF-α has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-αmutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-α, which binds to the murine TNF-R55 but not to the murine TNF-R75, exhibits retained antitumor activity and reduced systemic toxicity in mice compared with murine TNF-α, which binds to both murine TNF receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo.

Amino Acid Sequence

MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F

包装清单:

产品编号

产品名称

包装

JP527-10μg

Recombinant Human TNF-α/TNFSF2, Variant

10μg

JP527-100μg

Recombinant Human TNF-α/TNFSF2, Variant

100μg

JP527-500μg

Recombinant Human TNF-α/TNFSF2, Variant

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human TNF-α/TNFSF2

发表于由

产品编号

JP518-10μg
Recombinant Human TNF-α/TNFSF2
10μg

669

JP518-100μg
Recombinant Human TNF-α/TNFSF2
100μg

3075

JP518-1mg
Recombinant Human TNF-α/TNFSF2
1mg

9825

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

7124

JP1375

E. coli

158aa

17.5kDa

－

About this protein

Name

Recombinant Human TNF-α/TNFSF2 (Recombinant Human Tumor Necrosis Factor-α/TNFSF2; rHuTNF-α/TNFSF2)；重组人肿瘤坏死因子α/TNFSF2

Synonyms

Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, 10mM Nacl, pH7.0.

Endotoxin

Less than 1EU/μg of rHuTNF-α/TNFSF2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Amino Acid Sequence

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

包装清单:

产品编号

产品名称

包装

JP518-10μg

Recombinant Human TNF-α/TNFSF2

10μg

JP518-50μg

Recombinant Human TNF-α/TNFSF2

50μg

JP518-100μg

Recombinant Human TNF-α/TNFSF2

100μg

JP518-1mg

Recombinant Human TNF-α/TNFSF2

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

抗体/试剂/诊断抗体原料,肿瘤坏死因子-α/TNFα抗体 | RabbitAnti-TNF alpha antibody

发表于由

品牌：Bioss/博奥森 | 货号：JP-2081R

产品编号 JP-2081R
英文名称 TNF alpha
中文名称 肿瘤坏死因子-α/TNFα抗体
别    名 Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha.  
Specific References  (82)     |     JP-2081R has been referenced in 82 publications.
111 [IF=4.58] Wang, Xing, et al. “Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway.” Biochemical Pharmacology (2013).  WB ;  Rat.  222

111 [IF=3.31] Morishita, Seita, et al. ʺSystemic Simvastatin Rescues Retinal Ganglion Cells from Optic Nerve Injury Possibly through Suppression of Astroglial NF-κB Activation.ʺ PloS one 9.1 (2014): e84387.  IHC-F ;  Rat.  222

111 [IF=2.46] Wang, Shan, et al. “5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts.” Ultrasound in Medicine & Biology (2014).  IHC-P ;  Mouse.  222

111 [IF=2.71] Sun, Chao, et al. “Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue.” BioMed Research International 2015 (2015).  WB ;  Rat.  222

111 [IF=1.55] Wang, Yi, et al. “Targeted knockout of TNF‑α by injection of lentivirus‑mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats.” Molecular Medicine Reports.  other ;  

111 [IF=1.02] Xu, Qiang, et al. “Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus.” BMC Ophthalmology 15.1 (2015): 126.  IHC-P ;  Rat.  222

111 [IF=3.84] Yue, Yuan, et al. “Synergistic inhibitory effects of naproxen in combination with magnolol on TPA-induced skin inflammation in mice.” RSC Advances6.44 (2016): 38092-38099.  IHC-P ;  Mouse.  222

111 [IF=1.27] Ji, Mingli, et al. “Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits.”Experimental and Therapeutic Medicine 12.1 (2016): 51-58.  IHC-P ;  Rabbit.  222

111 [IF=2.6] Shi, Xiaowei, et al. “Panax notoginseng saponins administration modulates pro-/anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats.” Metabolic Brain Disease (2016): 1-13.  IHC-P ;  Rat.  222

111 [IF=1.56] Du, Jinghua, et al. “TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice.” Molecular medicine reports 13.3 (2016): 2159-2166.  WB ;  Mouse.  222

111 [IF=5.19] Han, Sheng, et al. “LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4.” Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.  WB ;  Human.  222

111 [IF=2.37] Dong, Min, et al. “AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE−/− mice.” Biochemical and Biophysical Research Communications (2017).  IHC-F ;  Mouse.  222

111 [IF=3.49] Wang, Xinhong, et al. “Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation.” Cytokine (2017).  IHC-P ;  Mouse.  222

111 [IF=3.88] Luo et al. Maternal Bisphenol A Diet Induces Anxiety-like Behavior in Female Juvenile with Neuroimmune Activation. (2014) Toxicol.Sc.  WB ;  Mouse.  222
PubMed:24824810

111 [IF=3.257] Fang et al. Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption. (2013) Int.J.Mol.Sc. 14:10343-54  WB ;  Rabbit.  222
PubMed:23685868

111 [IF=1.69] Li et al. Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. (2017) Mol.Med.Rep. 16:4455-4462  WB ;  Human, Rat.  222
PubMed:28791384

111 [IF=1.92] Qian et al. FSH aggravates bone loss in ovariectomised rats with experimental periapical periodontitis. (2016) Mol.Med.Rep. 14:2997-3006  IHC ;  Rat.  222
PubMed:27510616

111 [IF=3.189] Kim,et al.Local administration of nuclear factor of activated T cells (NFAT) c1 inhibitor to suppress early resorption and inflammation induced by bone morphogenetic protein-2.(2018) Journal of Biomedical Materials Research. Part A. 106:1299-1310.  IHC-P ;  Rat.  222
PubMed:29316218

111 [IF=2.995] Ding,et al.ZEB2 Attenuates LPS-Induced Inflammation by the NF-κB Pathway in HK-2 Cells.(2018) Inflammation. 41:722-731.  WB ;  Human.  222

111 [IF=1.759] Hashimoto,et al.The Effect of a High-Fat Diet on the Development of Abdominal Aortic Aneurysm in a Vascular Hypoperfusion-Induced Animal Model.(2018) Journal of Vascular Research. 55:63-74.  IHC-F ;  Rat.  222
PubMed:29393228

111 [IF=1.706] Wang,et al.Cx43 and NMDA receptors changes in UL122 genetically modified mice hippocampus: a mechanism for spatial memory impairment.(2018) International Journal of Clinical and Experimental Pathology. 11:129-1379.  IHC-P ;  Mouse.  222

111 [IF=5.25] Lennikov et al. Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis. (2018) Angiogenesis.  IHC-P ;  Rat.  222
PubMed:29332242

111 [IF=1.75] Bertuglia et al. Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career. (2016) BMC.Vet.Res. 12:246  ELISA ;  Horse.  222
PubMed:27821120

111 [IF=1.69] Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302  WB ;  Mouse.  222
PubMed:27035565

111 [IF=2.784] Sikandan A et al. Ashwagandha root extract exerts anti‑inflammatory effects in HaCaT cells by inhibiting the MAPK/NF‑κB pathways and by regulating cytokines.Int J Mol Med. 2018 Jul;42(1):425-434.  WB ;  Human.  222
PubMed:29620265

111 [IF=5.296] Cao Y et al. Regulatory role of IKKɑ in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages. J Mol Cell Cardiol. 2018 Oct;123:1-12.   IHC-P ;  Mouse.  222
PubMed:30153439

111 [IF=2.05] Neupane S et al. Focal localization of inflammatory cytokines and neurotrophins in a tongue chronic injury model.(2018)Arch Oral Biol. 9:22-30.  IHC-P ;  Mouse.  222
PubMed:30590230

111 [IF=3.547] Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.  IHC-P ;  Mouse.  222
PubMed:30685357

111 [IF=3.44] Luo Y et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull. 2019 Mar;146:320-326.  WB ;  Rat.  222
PubMed:30716395

111 [IF=3.986] Jena PK et al. Long-term Western diet intake leads to dysregulated bile acid signaling and dermatitis with Th2 and Th17 pathway features in mice. J Dermatol Sci. 2019 Jun 8. pii: JP-923-1811(19)30145-8.  WB ;  Mouse.  222
PubMed:31213388

研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  生长因子和激素  细胞因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 17/26kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TNF-alpha: 181-235/235 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]

Function:
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subunit:
Homotrimer. Interacts with SPPL2B.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.
Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein.
Tumor necrosis factor, soluble form: Secreted.
C-domain 1: Secreted.
C-domain 2: Secreted.

Post-translational modifications:
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

DISEASE:
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Similarity:
Belongs to the tumor necrosis factor family.

SWISS:
P01375

Gene ID:
7124

Database links:

Entrez Gene: 7124 Human

Entrez Gene: 21926 Mouse

Omim: 191160 Human

SwissProt: P01375 Human

SwissProt: P06804 Mouse

Unigene: 241570 Human

Unigene: 1293 Mouse

生长因子和激素（ Growth Factor and Hormones）
肿瘤坏死因子-TNF-alpha:是巨嗜细胞感染时或应激情况下自然产生的一种十分活跃的生物活性因子，可能与干扰素协同作用杀伤瘤细胞。

产品图片
Sample: Liver (mouse) Lysate at 40 ug
Primary: Anti-TNF alpha(JP-2081R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/26kD
Observed band size: 25 kD

Sample: Diseased lung(mouse) at 30ug;
Primary: Anti-TNF alpha (JP-2081R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size :17/28 kD
Observed band size :17/28 kD


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