描述
Enhanced Potassium Green-2 TMA+ Salt 钾离子指示探针
产品标签
EPG-2 salt,Asante Potassium Green-2 TMA Salt,PBFI AM,SBFI AM钠离子指示剂/探针,Valinomycin缬氨霉素;
产品信息
产品名称 |
产品编号 |
规格 |
价格(元) |
Enhanced Potassium Green-2 TMA+ Salt钾离子指示探针 |
MX4532-500UG-A |
500μg |
5968 |
Enhanced Potassium Green-2 TMA+ Salt钾离子指示探针 |
MX4532-500UG-B |
10×50μg |
7218 |
产品描述
Enhanced Potassium Green-2 (EPG-2)是Asante Potassium Green-2(APG-2)的替代产品,是一种新型K+荧光探针,具有良好光稳定性和宽动力学范围等理想特征,正好弥补传统K+荧光探针PBFI AM(# MX4510)的缺陷。EPG-2被可见光激发,最佳激发波长为517nm,但也能被传统波长488nm激发检测。虽然不是比率型探针,但其宽广的动力学范围使其甚至能检测到变化很小的K+浓度。
本品是EPG-2 TMA+ Salt,不具有细胞膜渗透性,不可通过简单孵育加载细胞。能被可见光激发,最大激发波长为526nm,发射波长为546nm。
产品特性
1) 分子式:C58H85Cl2N5O13
2) 分子量:1131 g/mol
3) 纯度:≥85%(HPLC)
4) 荧光光谱:Ex/ Em =526/546nm(in methanol)5) 吸收光谱:Amax=524nm
6) 摩尔吸光系数:85,000 M-1 cm-1
7) 解离常数(Kd):18mM
8) 溶解性:溶于H2O(1mg/ml)
9) 化学结构式:
保存与运输方法
保存:-20ºC避光干燥保存,至少1年有效。
运输:冰袋运输。
注意事项
1) EPG-2 TMA+ Salt易溶于水,可用水或生理缓冲液溶解配制成母液,小量分装置于-20℃干燥避光保存,避免反复冻融,
2) 为了您的安全和健康,请穿实验服并戴一次性手套操作。
参考文献
Rimmele TS et al. A Novel Optical Intracellular Imaging Approach for Potassium Dynamics in Astrocytes. PLoS One. 2014 Oct 2;9(10):e109243.
Bazzigaluppi P et al. Wide field fluorescent imaging of extracellular spatiotemporal potassium dynamics in vivo. Neuroimage. 2015 Jan 1;104:110-6.
Zhang L et al. Excess iron stress reduces root tip zone growth through nitric oxide‐mediated repression of potassium homeostasis in Arabidopsis. New Phytol. 2018 Jul;219(1):259-274.
Chen X et al. Pyroptosis is driven by non-selective gasdermin-D pore and its morphology is different from MLKL channel-mediated necroptosis. Cell Res. 2016 Sep;26(9):1007-20.
Wang F et al. Revealing the roles of GORK channels and NADPH oxidase in acclimation to hypoxia in Arabidopsis. J Exp Bot. 2017 Jun 1;68(12):3191-3204.
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