标签归档：recombinant

Recombinant Human IL-12

发表于由

产品编号

JJP54-10μg
Recombinant Human IL-12
10μg

1671

JJP54-1mg
Recombinant Human IL-12
1mg

34065

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3592

JP20459

CHO

503aa

75kDa

－

About this Protein

Name

Recombinant Human IL-12 (Recombinant Human Interleukin-12; rHuIL-12)；重组人白细胞介素12

Synonyms

CLMF1; IL12A; NKSF1; CLMF JP30; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit JP30; IL-12, subunit JP30; interleukin 12, JP30; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, JP30); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; JP30; JP30

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-12 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-12 (IL-12), also known as NKSF or CLMF, is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the JP30 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and JP30 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Amino Acid Sequence

JP30 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

包装清单:

产品编号

产品名称

包装

JJP54-2μg

Recombinant Human IL-12

2μg

JJP54-10μg

Recombinant Human IL-12

10μg

JJP54-1mg

Recombinant Human IL-12

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-11

发表于由

产品编号

JJJP5-10μg
Recombinant Human IL-11
10μg

1671

JJJP5-100μg
Recombinant Human IL-11
100μg

10650

JJJP5-1mg
Recombinant Human IL-11
1mg

34065

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3589

JP20809

E. coli

179aa

19.1kDa

－

About this protein

Name

Recombinant Human IL-11 (Recombinant Human Interleukin-11; rHuIL-11)；重组人白细胞介素11

Synonyms

AGIF; Adipogenesis inhibitory factor; AGIFoprelvekin; IL-11Oprelvekin; interleukin 11; interleukin-11

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine B9-11 cells is less than 1ng/ml, corresponding to a specific activity of >1.0×106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-11 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-11 (IL-11) is encoded by the IL11 gene and is a member of IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. It is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. The functions of IL-11 are that it directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production.

Amino Acid Sequence

MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL

包装清单:

产品编号

产品名称

包装

JJJP5-2μg

Recombinant Human IL-11

2μg

JJJP5-10μg

Recombinant Human IL-11

10μg

JJJP5-100μg

Recombinant Human IL-11

100μg

JJJP5-1mg

Recombinant Human IL-11

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-10

发表于由

产品编号

JJP55-10μg
Recombinant Human IL-10
10μg

1671

JJP55-100μg
Recombinant Human IL-10
100μg

10650

JJP55-1mg
Recombinant Human IL-10
1mg

34065

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3586

JP20301

E. coli

160aa

18.6kDa

－

About this protein

Name

Recombinant Human IL-10 (Recombinant Human Interleukin-10; rHuIL-10)；重组人白细胞介素10

Synonyms

CSIF; GVHDS; IL-10; IL10A; TGIF

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1ng/ml, corresponding to a specific activity of >1.0×106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-10 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10Rα/IL-10R1 chains, resulting in recruitment of two IL-10Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

Amino Acid Sequence

SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

包装清单:

产品编号

产品名称

包装

JJP55-2μg

Recombinant Human IL-10

2μg

JJP55-10μg

Recombinant Human IL-10

10μg

JJP55-100μg

Recombinant Human IL-10

100μg

JJP55-1mg

Recombinant Human IL-10

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-9

发表于由

产品编号

JJJP50-2μg
Recombinant Human IL-9
2μg

–

JJJP50-10μg
Recombinant Human IL-9
10μg

1671

JJJP50-100μg
Recombinant Human IL-9
100μg

10650

JJJP50-500μg
Recombinant Human IL-9
500μg

18780

JJJP50-1mg
Recombinant Human IL-9
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3578

P15248

E. coli

126aa

14.1kDa

－

About this protein

Name

Recombinant Human IL-9 (Recombinant Human Interleukin-9; rHuIL-9)；重组人白细胞介素9

Synonyms

Cytokine P40; HP40; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 0.2ng/ml, corresponding to a specific activity of >5.0×106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-9 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-9 (IL-9) is encoded by the IL9 gene and produced by T-cells and specifically by CD4+ helper cells. IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLVI) transformed T cell line. It functions through the IL-9 receptor, which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 can support the growth of IL-2 independent and IL-4 independent helper T-cells. Human IL-9 has approximately 56% amino acid sequence identity with murine IL-9. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.

Amino Acid Sequence

QGCPTLAGIL DINFLINKMQ EDPASKCHCS ANVTSCLCLG IPSDNCTRPC FSERLSQMTN TTMQTRYPLI FSRVKKSVEV LKNNKCPYFS CEQPCNQTTA GNALTFLKSL LEIFQKEKMR GMRGKI

包装清单:

产品编号

产品名称

包装

JJJP50-10μg

Recombinant Human IL-9

10μg

JJJP50-100μg

Recombinant Human IL-9

100μg

JJJP50-500μg

Recombinant Human IL-9

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-8, 77a.a/CXCL8

发表于由

产品编号

JJJP5-5μg
Recombinant Human IL-8, 77a.a/CXCL8
5μg

669

JJJP5-100μg
Recombinant Human IL-8, 77a.a/CXCL8
100μg

6138

JJJP5-1mg
Recombinant Human IL-8, 77a.a/CXCL8
1mg

19650

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3576

JP10145

E. coli

77aa

8.9kDa

－

About this protein

Name

Recombinant Human IL-8, 77a.a/CXCL8 (Recombinant Human Interleukin-8, 77a.a./CXCL8; rHuIL-8, 77a.a./CXCL8)；重组人白细胞介素8，77a.a./CXCL8

Synonyms

GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-8, 77a.a./CXCL8 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72a.a.) has a 5-10 fold higher activity on neutrophil activation, compared to IL-8 (77a.a.). IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.

Amino Acid Sequence

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

包装清单:

产品编号

产品名称

包装

JJJP5-5μg

Recombinant Human IL-8, 77a.a/CXCL8

5μg

JJJP5-25μg

Recombinant Human IL-8, 77a.a/CXCL8

25μg

JJJP5-100μg

Recombinant Human IL-8, 77a.a/CXCL8

100μg

JJJP5-1mg

Recombinant Human IL-8, 77a.a/CXCL8

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-8, 72a.a/CXCL8

发表于由

产品编号

JJP51-5μg
Recombinant Human IL-8, 72a.a/CXCL8
5μg

669

JJP51-100μg
Recombinant Human IL-8, 72a.a/CXCL8
100μg

6149

JJP51-500μg
Recombinant Human IL-8, 72a.a/CXCL8
500μg

10830

JJP51-1mg
Recombinant Human IL-8, 72a.a/CXCL8
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3576

JP10145

E. coli

72aa

8.4kDa

－

About this protein

Name

Recombinant Human IL-8, 72a.a/CXCL8 (Recombinant Human Interleukin-8, 72a.a./CXCL8; rHuIL-8, 72a.a./CXCL8)；重组人白细胞介素8，72a.a./CXCL8

Synonyms

GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-8, 72a.a./CXCL8 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72a.a.) has a 5-10-fold higher activity on neutrophil activation, compared to IL-8 (77a.a.). IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis.

Amino Acid Sequence

SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS

包装清单:

产品编号

产品名称

包装

JJP51-5μg

Recombinant Human IL-8, 72a.a/CXCL8

5μg

JJP51-100μg

Recombinant Human IL-8, 72a.a/CXCL8

100μg

JJP51-500μg

Recombinant Human IL-8, 72a.a/CXCL8

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-7

发表于由

产品编号

JJJP5-10μg
Recombinant Human IL-7
10μg

1671

JJJP5-100μg
Recombinant Human IL-7
100μg

10650

JJJP5-1mg
Recombinant Human IL-7
1mg

34065

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3574

P13232

E. coli

152aa

17.4kDa

－

About this protein

Name

Recombinant Human IL-7 (Recombinant Human Interleukin-7; rHuIL-7)；重组人白细胞介素7

Synonyms

Lymphopoietin-1; PBGF; IL-7interleukin-7; interleukin 7

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1EU/μg of rHuIL-7 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

Amino Acid Sequence

DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

包装清单:

产品编号

产品名称

包装

JJJP5-2μg

Recombinant Human IL-7

2μg

JJJP5-10μg

Recombinant Human IL-7

10μg

JJJP5-100μg

Recombinant Human IL-7

100μg

JJJP5-1mg

Recombinant Human IL-7

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-6

发表于由

产品编号

JJJP5-5μg
Recombinant Human IL-6
5μg

669

JJJP5-100μg
Recombinant Human IL-6
100μg

6138

JJJP5-1mg
Recombinant Human IL-6
1mg

19650

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3569

JP0105231

E. coli

183aa

20.7kDa

－

About this protein

Name

Recombinant Human IL-6 (Recombinant Human Interleukin-6; rHuIL-6)；重组人白细胞介素6

Synonyms

BSF-2; IFN-beta 2; MGI-2A; B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.
Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8ng/ml, corresponding to a specific activity of >1.25×106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1.0EU/μg of rHuIL-6 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and antiinflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL-6 shares about 40%a.a. sequence identity with mouse and rat IL-6 and it is equally active on mouse and rat cells.

Amino Acid Sequence

VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM

包装清单:

产品编号

产品名称

包装

JJJP5-5μg

Recombinant Human IL-6

5μg

JJJP5-20μg

Recombinant Human IL-6

20μg

JJJP5-100μg

Recombinant Human IL-6

100μg

JJJP5-1mg

Recombinant Human IL-6

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-5

发表于由

产品编号

JJP53-10μg
Recombinant Human IL-5
10μg

1671

JJP53-100μg
Recombinant Human IL-5
100μg

8800

JJP53-1mg
Recombinant Human IL-5
1mg

28162

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3567

JP0105113

E. coli

116aa

26.5kDa

－

About this protein

Name

Recombinant Human IL-5 (Recombinant Human Interleukin-5; rHuIL-5)；重组人白细胞介素5

Synonyms

BCDF mu; BCGFII; EDF; Eo-CSF; TRF; B-cell differentiation factor I; EDF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRFB cell differentiation factor I

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferationassay using human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.5, 3% trehalose, 0.1% Tween-80.

Endotoxin

Less than 1EU/μg of rHuIL-5 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

The IL-5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocytemacrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.

Amino Acid Sequence

MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

包装清单:

产品编号

产品名称

包装

JJP53-2μg

Recombinant Human IL-5

2μg

JJP53-10μg

Recombinant Human IL-5

10μg

JJP53-100μg

Recombinant Human IL-5

100μg

JJP53-1mg

Recombinant Human IL-5

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-4

发表于由

产品编号

JJJP5-5μg
Recombinant Human IL-4
5μg

669

JJJP5-100μg
Recombinant Human IL-4
100μg

6661

JJJP5-1mg
Recombinant Human IL-4
1mg

21288

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3565

JP0105112

E. coli

129aa

15kDa

－

About this protein

Name

Recombinant Human IL-4 (Recombinant Human Interleukin-4; rHuIL-4)；重组人白细胞介素4

Synonyms

BCDF; BSF1; B cell growth factor 1; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; Binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra; pitrakinra

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2ng/ml, corresponding to a specific activity of >5.0×106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-4 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The human IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds. The human IL-4 shares about 40%a.a. sequence identity with mouse/rat IL-4 and they are species-specific in their activities.

Amino Acid Sequence

HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS

包装清单:

产品编号

产品名称

包装

JJJP5-5μg

Recombinant Human IL-4

5μg

JJJP5-20μg

Recombinant Human IL-4

20μg

JJJP5-100μg

Recombinant Human IL-4

100μg

JJJP5-1mg

Recombinant Human IL-4

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-3

发表于由

产品编号

JJJP5-10μg
Recombinant Human IL-3
10μg

1671

JJJP5-100μg
Recombinant Human IL-3
100μg

6138

JJJP5-1mg
Recombinant Human IL-3
1mg

19650

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3562

JP0108700

E. coli

133aa

15kDa

－

About this protein

Name

Recombinant Human IL-3 (Recombinant Human Interleukin-3; rHuIL-3)；重组人白细胞介素3

Synonyms

Hematopoietic growth factor; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells.

Amino Acid Sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

包装清单:

产品编号

产品名称

包装

JJJP5-2μg

Recombinant Human IL-3

2μg

JJJP5-10μg

Recombinant Human IL-3

10μg

JJJP5-100μg

Recombinant Human IL-3

100μg

JJJP5-1mg

Recombinant Human IL-3

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-2

发表于由

产品编号

JP515-10μg
Recombinant Human IL-2
10μg

669

JP515-100μg
Recombinant Human IL-2
100μg

1612

JP515-1mg
Recombinant Human IL-2
1mg

5235

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3558

JJJJP6

E. coli

133aa

15.4kDa

－

About this protein

Name

Recombinant Human IL-2 (Recombinant Human Interleukin-2; rHuIL-2)；重组人白细胞介素2

Synonyms

aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH5.0.

Endotoxin

Less than 1EU/μg of rHuIL-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.

Category

Cytokine

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL-2Rα, 75kDa IL-2Rβ, and 64kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.

Amino Acid Sequence

APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

包装清单:

产品编号

产品名称

包装

JP515-10μg

Recombinant Human IL-2

10μg

JP515-50μg

Recombinant Human IL-2

50μg

JP515-100μg

Recombinant Human IL-2

100μg

JP515-1mg

Recombinant Human IL-2

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-1 RA

发表于由

产品编号

JP511-100μg
Recombinant Human IL-1 RA
100μg

2255

JP511-500μg
Recombinant Human IL-1 RA
500μg

3980

JP511-1mg
Recombinant Human IL-1 RA
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3557

P18510

E. coli

152aa

17.1kDa

－

About this Protein

Name

Recombinant Human IL-1 RA (Recombinant Human Interleukin-1 Receptor Antagonist; rHuIL-1RA)；重组人白细胞介素1受体拮抗剂

Synonyms

IL-1F3; IL1RN; DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; Interleukin 1 receptor antagonist; IRAP; MVCD4

Purity

>96% by SDS-PAGE and HPLC analyses

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40ng/ml, corresponding to a specific activity of >2.5×104IU/mg in the presence of 50pg/ml rHuIL-1α.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1EU/μg of rHuIL-1RA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-1 receptor antagonist (IL-1RA) is a member of the IL-1 family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL-1β. This protein inhibits the activities of interleukin 1, alpha (IL-1α) and interleukin 1, beta (IL-1β), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-RA. The regulated expression of IL-1RA in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1RA is markedly enhanced by IL-1, TNF-α, or PDGF. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α.

Amino Acid Sequence

RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

包装清单:

产品编号

产品名称

包装

JP511-100μg

Recombinant Human IL-1 RA

100μg

JP511-500μg

Recombinant Human IL-1 RA

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-1β

发表于由

产品编号

JJP506-10μg
Recombinant Human IL-1β
10μg

1671

JJP506-100μg
Recombinant Human IL-1β
100μg

7987

JJP506-1mg
Recombinant Human IL-1β
1mg

25545

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3553

JP1584

E. coli

153aa

17.3kDa

－

About this protein

Name

Recombinant Human IL-1β (Recombinant Human Interleukin-1β; rHuIL-1β)；重组人白细胞介素1β

Synonyms

IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of >1.0×109IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-1β as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL-1β shares 96% amino acid sequence identity with rhesus and 67%-78% with canine, mouse and rat IL-1β.

Amino Acid Sequence

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

包装清单:

产品编号

产品名称

包装

JJP506-2μg

Recombinant Human IL-1β

2μg

JJP506-10μg

Recombinant Human IL-1β

10μg

JJP506-100μg

Recombinant Human IL-1β

100μg

JJP506-1mg

Recombinant Human IL-1β

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human IL-1α

发表于由

产品编号

JJP502-10μg
Recombinant Human IL-1α
10μg

1671

JJP502-100μg
Recombinant Human IL-1α
100μg

7987

JJP502-1mg
Recombinant Human IL-1α
1mg

25545

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3552

JP1583

E. coli

159aa

18kDa

－

About this protein

Name

Recombinant Human IL-1α (Recombinant Human Interleukin-1α; rHuIL-1α)；重组人白细胞介素1α

Synonyms

IL1A, IL-1F1, Hematopoietin-1, IL1, IL-1 alpha, IL-1A, IL1-ALPHA, IL1F1hematopoietin-1, interleukin-1 alpha, preinterleukin 1 alpha, pro-interleukin-1-alpha

Purity

>97% by SDS-PAGE and HPLC analyses

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of >1.0×109IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1EU/μg of rHuIL-1α as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar identical biological properties. Among various species, the amino acid sequence of mature IL-1α is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. IL-1α recently started to find effective application in cosmetic and dermatological formulations, which allow to significantly harmonizing derma architecture.

Amino Acid Sequence

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

包装清单:

产品编号

产品名称

包装

JJP502-2μg

Recombinant Human IL-1α

2μg

JJP502-10μg

Recombinant Human IL-1α

10μg

JJP502-100μg

Recombinant Human IL-1α

100μg

JJP502-1mg

Recombinant Human IL-1α

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

胰蛋白酶（Trypsin 1:250胰酶），rypsin-EDTA (0.25%) CAS：9002-07-7

发表于由

Trypsin 1:250胰酶；Collagenase NB 4胶原酶NB4（标准级别）；Trypsin-EDTA (0.25%)；Dispase II中性蛋白酶；Roche；CAS：9001-12-1；

胰蛋白酶（胰酶，Trypsin），CAS：9002-07-7，来源于胰腺的一种丝氨酸蛋白酶，由223个氨基酸残基组成的单链多肽，底物特异性是带正电荷的赖氨酸和精氨酸侧链。胰酶主要切割赖氨酸和精氨酸羧基端，当两者之一紧随为脯氨酸的情况除外。另外，当切割位点任一边紧邻酸性残基，胰酶水解速率也会减缓。

胰蛋白酶（胰酶，Trypsin），以无活性的胰蛋白酶原（Trypsinogen）形式分泌于胰腺中，通过切割其末端六肽而得到活化，产生天然单链形式的β-胰酶（β-Trypsin），随后进行有限的自发裂解产生具水解活性的α-Trypsin（由二硫键共价连接的二条肽链组成）。胰酶水解活性可被多种化合物所抑制，有1）来源于胰腺、大豆、青豆、蛋清的天然胰蛋白酶抑制剂；2）银离子；3）有机磷化合物，如氟磷酸异丙酯DFP；4）特定蛋白酶抑制剂，如AEBSF、抗蛋白酶、抑肽酶、PMSF、TLCK等；

本品是毕赤酵母重组表达，氨基酸序列与猪胰腺来源的胰蛋白酶一致，具有与天然胰蛋白酶相同的酶学特性，可替代天然胰蛋白酶应用于各种生物技术过程，包括：重组胰岛素及类似物生产，细胞培养与发酵，蛋白质的酶解、测序，以及各种组织的细胞分离等。本品重组表达，无动物源性，天然缺乏糜蛋白酶活性，这种高质量的胰蛋白酶适用于蛋白质组学研究。

本品以冻干粉形式提供，比活力≥3800 USP units/mg protein。

产品特性

1） EC NO：3.4.21.4

2） CAS NO：9002-07-7

3） 同义名：Recombinant Trypsin, porcine sequence expressed inPichia pastoris;重组猪胰酶；

4） 来源：毕赤酵母

5） 纯度：单一主条带（SDS-PAGE）

6） 外观：类白色或白色冻干粉末

7） 溶解性：溶于1mM HCl（1mg/ml）

8） 比活力（胰蛋白酶）：≥3800 USP units/mg protein

9） 特异性：切割精氨酸和赖氨酸羧基端肽键

保存与运输方法

保存：-20℃干燥保存，至少2年有效。

运输：冰袋运输。

使用方法

1. 产品溶解

储存液制备：溶于1mM HCl（pH 3）配制母液，充分溶解，-20℃分装冻存，至少稳定保存~1年。加入Ca2+（20mM）能延缓胰蛋白酶的自溶和维持胰蛋白酶在溶液内的稳定性。

2. 产品用法

使用时，将储存液稀释在50mM NH4HCO3, pH 7.8或其它的合适缓冲液内。

胰蛋白酶：目的蛋白=1:50~1:1000，最适pH 7.0-9.0，最适温度：25-35℃。需注意，本品的活性受丝氨酸蛋白酶抑制剂比如：PMSF的抑制，金属离子螯合剂比如：EDTA的抑制。

注意事项

1）胰蛋白冻干粉产品常遇到的活性定义及其转换，

1 BAEE Unit：在25℃，pH 7.6的条件下，以BAEE为底物，3.2 ml的反应体系中，A253吸光值每分钟会发生0.001的变化即为一个酶活单位。

1 TAME Unit： 在25℃，pH 8.2，0.001M Ca2+条件下，每分钟水解1μmol p-toluenesulfonyl-L-arginine methyl ester（TAME）的用量。

1 USP Unit：在指定条件下，每分钟引起吸光值发生0.003的变化的样品活性。

1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Unit

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

名称 规格
Trypsin 1:250, Powder (Porcine)猪源胰蛋白酶1:250（粉末） 25g
Trypsin (0.25%), Phenol Red胰酶溶液（0.25%）,含酚红 100ml
Trypsin-EDTA (0.25%), Phenol Red胰酶-EDTA溶液（0.25%），含酚红 100ml
Trypsin, TPCK Treated胰蛋白酶（TPCK处理）（组织培养级） 100mg
Trypsin, TPCK Treated, Sequencing Grade测序级胰蛋白酶（TPCK处理） 100mg
Trypsin 1:2500, Powder (Porcine)  猪源胰蛋白酶1:2500（粉末） 5g
Trypsin 1:2500, Powder (Bovine)  牛源胰蛋白酶1:2500（粉末） 1g
Trypsin from Porcine, Recombinant重组猪胰蛋白酶 10mg

 

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶

发表于由

重组赖氨酰内切酶；Recombinant Lysyl Endopeptidase；rLys-C；Trypsin 胰酶；EC NO：3.4.21.50；

产品信息

产品名称

 产品编号 规格

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶

 MX2015-10AU 10AU
Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 MX2015-100AU 100AU

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 MX2015-1000AU 1000AU

产品描述

重组赖氨酰内切酶（Recombinant Lysyl Endopeptidase, rLys-C）属于丝氨酸蛋白酶，特异性切割赖氨酸残基羧基端的肽键。Lys-C广泛用于各种生物技术过程中，如：蛋白质酶解生产多肽；GLP-1类药物制备等。生物活性受 DFP、PMSF、TLCK 抑制，不被TACK抑制。

本品为大肠杆菌重组表达所得，无外源性的病毒污染，生产过程中不使用任何动物源的原料。基于稳定的重组生产工艺，可批量生产，批次间几乎无差异，质量稳定，且纯度高，低内毒素水平。以冻干粉的形式提供，酶活力≥0.5AU /mg powder。rLys-C适用于重组胰岛素及类似物生产、GLP-1类似物生产、蛋白质质谱、测序、肽图谱分析；以及小分子蛋白或多肽生产等。

产品特性

1） EC NO：3.4.21.50
2） 同义名：Achromobacter proteinase I; Lysyl bond specific proteinase; Endoproteinase lys-c;
3） 级别：生化级（Biochemistry Grade）
4） 分子量：29±2.5 kDa（SDS-PAGE）
5） 纯度：单一条带（SDS-PAGE）
6） 外观：类白色或白色粉末
7） 最适pH：9.0-9.5
8） 等电点：6.9-7.0
9） 最适温度：30-37℃，50℃以上稳定性下降
10） 稳定性：在 4mol/L 尿素或0.2%SDS 溶液中 30℃孵育 6h后，生物活性未发生降低。稳定性较好。
11） 抑制剂：DFP、PMSF、TLCK
12） 酶活力：≥0.5AU /mg powder
13） 细菌内毒素：≤10EU/mg powder
14） 溶解性：溶于Tris-HCl

保存与运输方法

保存：-20℃干燥保存，至少2年有效。

运输：冰袋运输。

使用方法

  1. 产品溶解

储存液制备：溶于20-50mM Tris-HCl（pH 8.5-9.5）配制母液，充分溶解，-20℃分装冻存，数月稳定保存。配制好的储存液可承受反复冻融~5次。

  1. 产品用法

使用时，将储存液加入样本溶液，按照酶：蛋白=(1-100AU):1g的比例来优化加量，最适pH：9.0-9.5，最适温度30-37℃，反应时间2~24h。

【注意】：如果样本所含的无机盐过高（≥0.1M），会影响酶活。建议对样本进行脱盐后再进行酶解反应。若不能进行脱盐，建议通过增加酶量且延长孵育时间来达到所需效果。

注意事项

1）我司（金畔生物）可定制供应重组赖氨酰内切酶，有更大包装或特殊包装需求请与我们联系。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号 名称 规格
MX2002-25G Trypsin 1:250, Powder (Porcine) 猪源胰蛋白酶1:250（粉末） 25g
MX2003-100ML Trypsin (0.25%), Phenol Red 胰酶溶液（0.25%）,含酚红 100ml
MX2004-100ML Trypsin-EDTA (0.25%), Phenol Red 胰酶-EDTA溶液（0.25%）,含酚红 100ml
MX2005-100MG Trypsin, TPCK Treated 胰蛋白酶（TPCK处理）（组织培养级） 100mg
MX2006-100MG Trypsin, TPCK Treated, Sequencing Grade 测序级胰蛋白酶（TPCK处理） 100mg
MX2011-5G Trypsin 1:2500, Powder (Porcine)  猪源胰蛋白酶1:2500（粉末） 5g
MX2012-1G Trypsin 1:2500, Powder (Bovine)  牛源胰蛋白酶1:2500（粉末） 1g
MX2015-10AU Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 10AU

 

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶

发表于由

重组赖氨酰内切酶；Recombinant Lysyl Endopeptidase；rLys-C；Trypsin 胰酶；EC NO：3.4.21.50；

产品信息

产品名称

 产品编号 规格

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶

 MX2015-10AU 10AU
Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 MX2015-100AU 100AU

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 MX2015-1000AU 1000AU

产品描述

重组赖氨酰内切酶（Recombinant Lysyl Endopeptidase, rLys-C）属于丝氨酸蛋白酶，特异性切割赖氨酸残基羧基端的肽键。Lys-C广泛用于各种生物技术过程中，如：蛋白质酶解生产多肽；GLP-1类药物制备等。生物活性受 DFP、PMSF、TLCK 抑制，不被TACK抑制。

本品为大肠杆菌重组表达所得，无外源性的病毒污染，生产过程中不使用任何动物源的原料。基于稳定的重组生产工艺，可批量生产，批次间几乎无差异，质量稳定，且纯度高，低内毒素水平。以冻干粉的形式提供，酶活力≥0.5AU /mg powder。rLys-C适用于重组胰岛素及类似物生产、GLP-1类似物生产、蛋白质质谱、测序、肽图谱分析；以及小分子蛋白或多肽生产等。

产品特性

1） EC NO：3.4.21.50
2） 同义名：Achromobacter proteinase I; Lysyl bond specific proteinase; Endoproteinase lys-c;
3） 级别：生化级（Biochemistry Grade）
4） 分子量：29±2.5 kDa（SDS-PAGE）
5） 纯度：单一条带（SDS-PAGE）
6） 外观：类白色或白色粉末
7） 最适pH：9.0-9.5
8） 等电点：6.9-7.0
9） 最适温度：30-37℃，50℃以上稳定性下降
10） 稳定性：在 4mol/L 尿素或0.2%SDS 溶液中 30℃孵育 6h后，生物活性未发生降低。稳定性较好。
11） 抑制剂：DFP、PMSF、TLCK
12） 酶活力：≥0.5AU /mg powder
13） 细菌内毒素：≤10EU/mg powder
14） 溶解性：溶于Tris-HCl

保存与运输方法

保存：-20℃干燥保存，至少2年有效。

运输：冰袋运输。

使用方法

  1. 产品溶解

储存液制备：溶于20-50mM Tris-HCl（pH 8.5-9.5）配制母液，充分溶解，-20℃分装冻存，数月稳定保存。配制好的储存液可承受反复冻融~5次。

  1. 产品用法

使用时，将储存液加入样本溶液，按照酶：蛋白=(1-100AU):1g的比例来优化加量，最适pH：9.0-9.5，最适温度30-37℃，反应时间2~24h。

【注意】：如果样本所含的无机盐过高（≥0.1M），会影响酶活。建议对样本进行脱盐后再进行酶解反应。若不能进行脱盐，建议通过增加酶量且延长孵育时间来达到所需效果。

注意事项

1）我司（金畔生物）可定制供应重组赖氨酰内切酶，有更大包装或特殊包装需求请与我们联系。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号 名称 规格
MX2002-25G Trypsin 1:250, Powder (Porcine) 猪源胰蛋白酶1:250（粉末） 25g
MX2003-100ML Trypsin (0.25%), Phenol Red 胰酶溶液（0.25%）,含酚红 100ml
MX2004-100ML Trypsin-EDTA (0.25%), Phenol Red 胰酶-EDTA溶液（0.25%）,含酚红 100ml
MX2005-100MG Trypsin, TPCK Treated 胰蛋白酶（TPCK处理）（组织培养级） 100mg
MX2006-100MG Trypsin, TPCK Treated, Sequencing Grade 测序级胰蛋白酶（TPCK处理） 100mg
MX2011-5G Trypsin 1:2500, Powder (Porcine)  猪源胰蛋白酶1:2500（粉末） 5g
MX2012-1G Trypsin 1:2500, Powder (Bovine)  牛源胰蛋白酶1:2500（粉末） 1g
MX2015-10AU Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 10AU

 

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶

发表于由

重组赖氨酰内切酶；Recombinant Lysyl Endopeptidase；rLys-C；Trypsin 胰酶；EC NO：3.4.21.50；

产品信息

产品名称

 产品编号 规格

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶

 MX2015-10AU 10AU
Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 MX2015-100AU 100AU

Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 MX2015-1000AU 1000AU

产品描述

重组赖氨酰内切酶（Recombinant Lysyl Endopeptidase, rLys-C）属于丝氨酸蛋白酶，特异性切割赖氨酸残基羧基端的肽键。Lys-C广泛用于各种生物技术过程中，如：蛋白质酶解生产多肽；GLP-1类药物制备等。生物活性受 DFP、PMSF、TLCK 抑制，不被TACK抑制。

本品为大肠杆菌重组表达所得，无外源性的病毒污染，生产过程中不使用任何动物源的原料。基于稳定的重组生产工艺，可批量生产，批次间几乎无差异，质量稳定，且纯度高，低内毒素水平。以冻干粉的形式提供，酶活力≥0.5AU /mg powder。rLys-C适用于重组胰岛素及类似物生产、GLP-1类似物生产、蛋白质质谱、测序、肽图谱分析；以及小分子蛋白或多肽生产等。

产品特性

1） EC NO：3.4.21.50
2） 同义名：Achromobacter proteinase I; Lysyl bond specific proteinase; Endoproteinase lys-c;
3） 级别：生化级（Biochemistry Grade）
4） 分子量：29±2.5 kDa（SDS-PAGE）
5） 纯度：单一条带（SDS-PAGE）
6） 外观：类白色或白色粉末
7） 最适pH：9.0-9.5
8） 等电点：6.9-7.0
9） 最适温度：30-37℃，50℃以上稳定性下降
10） 稳定性：在 4mol/L 尿素或0.2%SDS 溶液中 30℃孵育 6h后，生物活性未发生降低。稳定性较好。
11） 抑制剂：DFP、PMSF、TLCK
12） 酶活力：≥0.5AU /mg powder
13） 细菌内毒素：≤10EU/mg powder
14） 溶解性：溶于Tris-HCl

保存与运输方法

保存：-20℃干燥保存，至少2年有效。

运输：冰袋运输。

使用方法

  1. 产品溶解

储存液制备：溶于20-50mM Tris-HCl（pH 8.5-9.5）配制母液，充分溶解，-20℃分装冻存，数月稳定保存。配制好的储存液可承受反复冻融~5次。

  1. 产品用法

使用时，将储存液加入样本溶液，按照酶：蛋白=(1-100AU):1g的比例来优化加量，最适pH：9.0-9.5，最适温度30-37℃，反应时间2~24h。

【注意】：如果样本所含的无机盐过高（≥0.1M），会影响酶活。建议对样本进行脱盐后再进行酶解反应。若不能进行脱盐，建议通过增加酶量且延长孵育时间来达到所需效果。

注意事项

1）我司（金畔生物）可定制供应重组赖氨酰内切酶，有更大包装或特殊包装需求请与我们联系。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号 名称 规格
MX2002-25G Trypsin 1:250, Powder (Porcine) 猪源胰蛋白酶1:250（粉末） 25g
MX2003-100ML Trypsin (0.25%), Phenol Red 胰酶溶液（0.25%）,含酚红 100ml
MX2004-100ML Trypsin-EDTA (0.25%), Phenol Red 胰酶-EDTA溶液（0.25%）,含酚红 100ml
MX2005-100MG Trypsin, TPCK Treated 胰蛋白酶（TPCK处理）（组织培养级） 100mg
MX2006-100MG Trypsin, TPCK Treated, Sequencing Grade 测序级胰蛋白酶（TPCK处理） 100mg
MX2011-5G Trypsin 1:2500, Powder (Porcine)  猪源胰蛋白酶1:2500（粉末） 5g
MX2012-1G Trypsin 1:2500, Powder (Bovine)  牛源胰蛋白酶1:2500（粉末） 1g
MX2015-10AU Recombinant Lysyl Endopeptidase (rLys-C) 重组赖氨酰内切酶 10AU

 

Insulin, Human Recombinant 重组人胰岛素

发表于由

描述

Insulin, Human Recombinant 重组人胰岛素

产品标签

Insulin 胰岛素；Fibronectin 纤连蛋白；Fibrinogen 纤维蛋白原；降血糖激素；Diabetes 糖尿病（I型，II型）；Streptozotocin (STZ) 链脲佐菌素；CAS NO：11061-68-0；

产品信息

  产品名称   产品编号   规格   价格（元）
  Insulin, Human Recombinant 重组人胰岛素      MX0904-25MG   25mg   398
  Insulin, Human Recombinant 重组人胰岛素   MX0904-100MG   100mg   1298
  Insulin, Human Recombinant 重组人胰岛素   MX0904-500MG   500mg      4548
  Insulin, Human Recombinant 重组人胰岛素   MX0904-1000MG      1g   7928

产品描述

胰岛素（Insulin）是胰腺内胰岛β细胞分泌的一种由α和β双链构成的多肽激素，由51个氨基酸组成，分子量约5800 Da。两条链由两对链间二硫键连接，另外α链自身还含有一对链内二硫键。胰岛素是目前已知的机体内唯一能降低血糖的激素，不仅负责调控细胞对葡萄糖、氨基酸和脂肪酸的摄入，利用和储存，而且抑制糖原，蛋白质和脂肪的降解发生。

不同物种胰岛素功能大体相同，只是氨基酸组成上的差异。人胰岛素结构见下：

α链：

G-I-V-E-Q-C-C-T-S-I-C-S-L-Y-Q-L-E-N-Y-C-N

β链：

F-V-N-Q-H-L-C-G-S-H-L-V-E-A-L-Y-L-V-C-G-E-R-G-F-F-Y-T-P-K-T

本品为酵母菌重组表达，经亲和纯化所得，适合用作细胞培养添加物，典型工作浓度范围1-10μg/ml。

产品特性

1） CAS NO：11061-68-0

2） 分子式：C257H383N65O77S6

3） 分子量：5807.57

4） 外观：白色或类白色粉末

5） 等电点（PI）：5.3（天然蛋白）

6） 活性：≥15 U/mg

7） 纯度：95%-105%

8） 微生物限度：≤300cfu/g

9） 细菌内毒素：≤10EU/mg

10）溶解性：不溶解于中性pH，溶于稀醋酸或稀盐酸（pH 2-3）

11）阳离子残留：Zn≤1.0%（若实验需要去除锌离子，可将胰岛素溶解在稀醋酸内，加入过量EDTA螯合锌离子，然后等电点沉淀法重新纯化胰岛素）

12）氨基酸序列信息：

α链：G-I-V-E-Q-C-C-T-S-I-C-S-L-Y-Q-L-E-N-Y-C-N

β链：F-V-N-Q-H-L-C-G-S-H-L-V-E-A-L-Y-L-V-C-G-E-R-G-F-F-Y-T-P-K-T

保存与运输方法

保存：冻干粉-20℃保存，防止受潮，2年有效。

运输：冰袋运输。

常见问题

1. 选择什么溶剂来溶解胰岛素干粉？

胰岛素中性环境几乎不溶解，但可迅速溶解于稀酸或稀碱，测试数据表明胰岛素溶于0.01M HCl的浓度可达20 mg/ml。

2. 胰岛素的母液如何配制和保存？

将胰岛素溶于稀醋酸（1%）或稀盐酸（pH 2-3）配制1-10mg/ml的储存液，用低蛋白吸附滤膜对配制好的溶液进行过滤除菌，按照单次使用剂量，分装冻存于-20℃，避免反复冻融。至少6个月稳定。

【注意】：胰岛素虽可溶于125mM碳酸氢钠溶液，但不建议制备碱性储存液，原因在于高pH会导致发生脱酰胺和聚集反应的速率提高。胰岛素溶液不可进行高压灭菌处理。

3. 胰岛素的应用和使用浓度？

胰岛素中重组人胰岛素、牛胰岛素或猪胰岛素可用作细胞培养添加物。体外研究胰岛素长期被认为是用来调控大部分细胞生长和分化的关键因子。常用使用浓度范围是1-10μg/ml，主要根据细胞类型来决定。另外，对于要求无蛋白细胞培养体系，可将胰岛素固定在聚苯乙烯培养皿表面，以提高细胞的粘附性。

注意事项

为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

MX0902-25MG Insulin, from Bovine Pancreas 牛胰岛素 25mg
MX0903-25MG Insulin, from Porcine Pancreas 猪胰岛素 25mg
MX0904-25MG Insulin, Human Recombinant 重组人胰岛素 25mg
MX0905-100MG Bovine Transferrin (APO) 牛脱铁转铁蛋白 100mg
MX0906-100MG Bovine Transferrin (HOLO) 牛饱和铁转铁蛋白 100mg
MX0907-500U Thrombin, from Bovine Plasma 牛凝血酶 500U
MX0908-100U Human α-Thrombin (Factor IIa) 人α-凝血酶 100U
MX0909-100MG Bovine Fibrinogen 牛纤维蛋白原 100mg