CAS:9013-20-1 PE-Streptavidin PE标记链霉亲和素系列

Streptavidin 链霉亲和素(链亲和素);Avidin亲和素;链霉亲和素-生物素放大系统;Streptavidin Magbeads 链霉亲和素免疫磁珠;CAS:9013-20-1

产品描述

链霉亲和素(Streptavidin),分子量约为60kDa,来源于阿维丁链霉菌(Streptomyces avidinii)的一种细菌蛋白,高度特异性结合生物素(biotin),亲和常数(Kd)为10-15M。作为一种四聚体蛋白,可同时结合4个生物素分子,该功能与鸡蛋清来源的亲和素(avidin)具很高的相似性。然而,不同于亲和素,链霉亲和素是一种非糖基化蛋白,基本不带电荷,而亲和素在中性pH条件下呈碱性,比较而言链霉亲和素的非特异性结合远低于亲和素,这样检测时链霉亲和素的非特异性背景明显低很多。目前,生物素检测通常用链霉亲和素替代亲和素。

本品为PE标记的链霉亲和素(PE-Streptavidin),可由488nm的激光激发后,在最大发射波长578nm处检测发射荧光,可用于生物素标记的抗体、核酸、蛋白或其他生物素标记分子的检测。

本品以液体形式供货,浓度1mg/ml,缓冲液为0.01M PBS, pH 7.4, 0.09%(w/v) NaN3。

产品特征

1)同义名:PE-Conjugated Streptavidin; Streptavidin-Phycoerythrin; SAv-PE;

2)反应性:人、大鼠、小鼠、所有物种

3)浓度:1mg/ml(实际见包装标签)

4)应用:FC、ICFC

5)建议使用浓度:对于流式染色,建议使用浓度为0.5µg/test(细胞浓度调整为:106个/100µl),建议每次实验进行浓度优化以得到最佳染色结果。

保存与运输方法

保存:2-8℃避光保存,避免冻存。一年有效。

运输:冰袋运输。

注意事项

1)本品含叠氮钠,操作时请注意安全防护,不要直接接触眼睛、皮肤等。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

1、链霉亲和素(Streptavidin),Unconjugated

产品名称                                                                                   规格             外观              
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) 1ml 液体
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) 5ml 液体
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) 10ml 液体
Streptavidin, Recombinant 链霉亲和素(重组型) 10mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 50mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 100mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 500mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 1g 粉末

2、链霉亲和素(Streptavidin),Conjugated

产品名称                                                                              规格             Ex/Em(nm)   
HRP-Streptavidin 100µl
Alkaline Phosphatase-Streptavidin 100µl
FITC-Streptavidin 500µg 488/520
PE-Streptavidin 500µg 565/578
Alexa Fluor 405-Streptavidin 200µg 401/420
Alexa Fluor 488-Streptavidin 200µg 491/514
Alexa Fluor 555-Streptavidin 200µg 559/569
Alexa Fluor 594-Streptavidin 200µg 592/614
Alexa Fluor 647-Streptavidin 200µg 654/674
Alexa Fluor 680-Streptavidin 200µg 682/701
Alexa Fluor 750-Streptavidin 200µg 753/779
Cy2-Streptavidin Cy2 1mg 492/510
Cy3-Streptavidin 1mg 550/570
Cy5-Streptavidin 1mg 650/670

3、链霉亲和素免疫磁珠(Streptavidin Magbeads)

产品名称                                                                                   规格           浓度                
Streptavidin Magnetic Beads 1 µm 链霉亲和素磁珠 1ml 10mg/ml
Streptavidin Magnetic Beads 1 µm 链霉亲和素磁珠 10ml 10mg/ml
Streptavidin Magnetic Beads 2 µm 链霉亲和素磁珠 1ml 10mg/ml
Streptavidin Magnetic Beads 2 µm 链霉亲和素磁珠 10ml 10mg/ml
Streptavidin Magnetic Beads 5 µm 链霉亲和素磁珠 1ml 10mg/ml
Streptavidin Magnetic Beads 5 µm 链霉亲和素磁珠 10ml 10mg/ml

PE-Streptavidin PE标记链霉亲和素 Streptavidin Magbeads 链霉亲和素免疫磁珠 9013-20-1

Streptavidin 链霉亲和素(链亲和素);Avidin亲和素;链霉亲和素-生物素放大系统;Streptavidin Magbeads 链霉亲和素免疫磁珠;CAS:9013-20-1

链霉亲和素(Streptavidin),分子量约为60kDa,来源于阿维丁链霉菌(Streptomyces avidinii)的一种细菌蛋白,高度特异性结合生物素(biotin),亲和常数(Kd)为10-15M。作为一种四聚体蛋白,可同时结合4个生物素分子,该功能与鸡蛋清来源的亲和素(avidin)具很高的相似性。然而,不同于亲和素,链霉亲和素是一种非糖基化蛋白,基本不带电荷,而亲和素在中性pH条件下呈碱性,比较而言链霉亲和素的非特异性结合远低于亲和素,这样检测时链霉亲和素的非特异性背景明显低很多。目前,生物素检测通常用链霉亲和素替代亲和素。

本品为PE标记的链霉亲和素(PE-Streptavidin),可由488nm的激光激发后,在最大发射波长578nm处检测发射荧光,可用于生物素标记的抗体、核酸、蛋白或其他生物素标记分子的检测。

本品以液体形式供货,浓度1mg/ml,缓冲液为0.01M PBS, pH 7.4, 0.09%(w/v) NaN3。

产品特征

1)同义名:PE-Conjugated Streptavidin; Streptavidin-Phycoerythrin; SAv-PE;

2)反应性:人、大鼠、小鼠、所有物种

3)浓度:1mg/ml(实际见包装标签)

4)应用:FC、ICFC

5)建议使用浓度:对于流式染色,建议使用浓度为0.5µg/test(细胞浓度调整为:106个/100µl),建议每次实验进行浓度优化以得到最佳染色结果。

保存与运输方法

保存:2-8℃避光保存,避免冻存。一年有效。

运输:冰袋运输。

注意事项

1)本品含叠氮钠,操作时请注意安全防护,不要直接接触眼睛、皮肤等。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

1、链霉亲和素(Streptavidin),Unconjugated

产品名称                                                                                   规格             外观              
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) 1ml 液体
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) 5ml 液体
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) 10ml 液体
Streptavidin, Recombinant 链霉亲和素(重组型) 10mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 50mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 100mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 500mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) 1g 粉末

2、链霉亲和素(Streptavidin),Conjugated

产品名称                                                                               规格             Ex/Em(nm)   
HRP-Streptavidin 100µl /
Alkaline Phosphatase-Streptavidin 100µl
FITC-Streptavidin 500µg 488/520
PE-Streptavidin 500µg 565/578
Alexa Fluor 405-Streptavidin 200µg 401/420
Alexa Fluor 488-Streptavidin 200µg 491/514
Alexa Fluor 555-Streptavidin 200µg 559/569
Alexa Fluor 594-Streptavidin 200µg 592/614
Alexa Fluor 647-Streptavidin 200µg 654/674
Alexa Fluor 680-Streptavidin 200µg 682/701
Alexa Fluor 750-Streptavidin 200µg 753/779
Cy2-Streptavidin Cy2 1mg 492/510
Cy3-Streptavidin 1mg 550/570
Cy5-Streptavidin 1mg 650/670

3、链霉亲和素免疫磁珠(Streptavidin Magbeads)

产品名称                                                                                   规格           浓度                
Streptavidin Magnetic Beads 1 µm 链霉亲和素磁珠 1ml 10mg/ml
Streptavidin Magnetic Beads 1 µm 链霉亲和素磁珠 10ml 10mg/ml
Streptavidin Magnetic Beads 2 µm 链霉亲和素磁珠 1ml 10mg/ml
Streptavidin Magnetic Beads 2 µm 链霉亲和素磁珠 10ml 10mg/ml
Streptavidin Magnetic Beads 5 µm 链霉亲和素磁珠 1ml 10mg/ml
Streptavidin Magnetic Beads 5 µm 链霉亲和素磁珠 10ml 10mg/ml

PE-Streptavidin PE标记链霉亲和素 链霉亲和素免疫磁珠 CAS:9013-20-1

Streptavidin 链霉亲和素(链亲和素);Avidin亲和素;链霉亲和素-生物素放大系统;Streptavidin Magbeads 链霉亲和素免疫磁珠;CAS:9013-20-1

产品信息

产品名称 产品编号                    规格                          
PE-Streptavidin PE标记链霉亲和素    MM50104-50UG 50µg
PE-Streptavidin PE标记链霉亲和素    MM50104-100UG 100µg
PE-Streptavidin PE标记链霉亲和素 MM50104-500UG 500µg

链霉亲和素(Streptavidin),分子量约为60kDa,来源于阿维丁链霉菌(Streptomyces avidinii)的一种细菌蛋白,高度特异性结合生物素(biotin),亲和常数(Kd)为10-15M。作为一种四聚体蛋白,可同时结合4个生物素分子,该功能与鸡蛋清来源的亲和素(avidin)具很高的相似性。然而,不同于亲和素,链霉亲和素是一种非糖基化蛋白,基本不带电荷,而亲和素在中性pH条件下呈碱性,比较而言链霉亲和素的非特异性结合远低于亲和素,这样检测时链霉亲和素的非特异性背景明显低很多。目前,生物素检测通常用链霉亲和素替代亲和素。

本品为PE标记的链霉亲和素(PE-Streptavidin),可由488nm的激光激发后,在最大发射波长578nm处检测发射荧光,可用于生物素标记的抗体、核酸、蛋白或其他生物素标记分子的检测。

本品以液体形式供货,浓度1mg/ml,缓冲液为0.01M PBS, pH 7.4, 0.09%(w/v) NaN3。

产品特征

1)同义名:PE-Conjugated Streptavidin; Streptavidin-Phycoerythrin; SAv-PE;

2)反应性:人、大鼠、小鼠、所有物种

3)浓度:1mg/ml(实际见包装标签)

4)应用:FC、ICFC

5)建议使用浓度:对于流式染色,建议使用浓度为0.5µg/test(细胞浓度调整为:106个/100µl),建议每次实验进行浓度优化以得到最佳染色结果。

保存与运输方法

保存:2-8℃避光保存,避免冻存。一年有效。

运输:冰袋运输。

注意事项

1)本品含叠氮钠,操作时请注意安全防护,不要直接接触眼睛、皮肤等。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

1、链霉亲和素(Streptavidin),Unconjugated

产品名称                                                                 产品编号                   规格             外观              
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) MM50001-1ML 1ml 液体
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) MM50001-5ML 5ml 液体
Streptavidin (1mg/ml) 链霉亲和素(1mg/ml) MM50001-10ML 10ml 液体
Streptavidin, Recombinant 链霉亲和素(重组型) MM50002-10MG 10mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) MM50002-50MG 50mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) MM50002-100MG 100mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) MM50002-500MG 500mg 粉末
Streptavidin, Recombinant 链霉亲和素(重组型) MM50002-1000MG 1g 粉末

2、链霉亲和素(Streptavidin),Conjugated

产品名称                                                          产品编号                     规格             Ex/Em(nm)   
HRP-Streptavidin MM50101-100UL 100µl /
Alkaline Phosphatase-Streptavidin MM50102-100UL 100µl /
FITC-Streptavidin MM50103-500UG 500µg 488/520
PE-Streptavidin MM50104-500UG 500µg 565/578
Alexa Fluor 405-Streptavidin MM50105-200UG 200µg 401/420
Alexa Fluor 488-Streptavidin MM50106-200UG 200µg 491/514
Alexa Fluor 555-Streptavidin MM50107-200UG 200µg 559/569
Alexa Fluor 594-Streptavidin MM50108-200UG 200µg 592/614
Alexa Fluor 647-Streptavidin MM50109-200UG 200µg 654/674
Alexa Fluor 680-Streptavidin MM50110-200UG 200µg 682/701
Alexa Fluor 750-Streptavidin MM50111-200UG 200µg 753/779
Cy2-Streptavidin Cy2 MM50112-1MG 1mg 492/510
Cy3-Streptavidin MM50113-1MG 1mg 550/570
Cy5-Streptavidin MM50114-1MG 1mg 650/670

3、链霉亲和素免疫磁珠(Streptavidin Magbeads)

产品名称                                                                     产品编号              规格           浓度                
Streptavidin Magnetic Beads 1 µm 链霉亲和素磁珠 MP3901-1ML 1ml 10mg/ml
Streptavidin Magnetic Beads 1 µm 链霉亲和素磁珠 MP3901-10ML 10ml 10mg/ml
Streptavidin Magnetic Beads 2 µm 链霉亲和素磁珠 MP3902-1ML 1ml 10mg/ml
Streptavidin Magnetic Beads 2 µm 链霉亲和素磁珠 MP3902-10ML 10ml 10mg/ml
Streptavidin Magnetic Beads 5 µm 链霉亲和素磁珠 MP3903-1ML 1ml 10mg/ml
Streptavidin Magnetic Beads 5 µm 链霉亲和素磁珠 MP3903-10ML 10ml 10mg/ml

BD 559763细胞凋亡试剂盒(PE和7-ADD标记)PE Annexin V Apoptosis Detectio

【简单介绍】

NameAnnexin V : PE Apoptosis Detection Kit IContentsAnnexin V-PE, 7-AAD, and Annexin V Binding BufferSize100 TestsRegulatory Status RUO

*,现货*格优惠,咨询

【详细说明】

 Technical Data Sheet

PE Annexin V Apoptosis Detection Kit I

Product Information

Material Number: 559763

Component: 51-66121E

Description: 10X Annexin V Binding Buffer

Size: 50 ml (1 ea)

Storage Buffer: Aqueous buffered solution containing no preservative.

Component: 51-68981E

Description: 7-AAD

Size: 2.0 ml (1 ea)

Vol. per Test: 5 μl

Storage Buffer: Aqueous buffered solution containing fetal bovine serum and ≤0.09% sodium

azide.

Component: 51-65875X

Description: PE Annexin V

Size: 0.5 ml (1 ea)

Vol. per Test: 5 μl

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Description

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The

apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment,

condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features.

In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma

membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding

protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including

Phycoerythrin (PE). This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are

undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, PE Annexin V staining can identify apoptosis at an

earlier stage than assays based on nuclear changes such as DNA fragmentation.

PE Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either

apoptotic or necrotic processes. Therefore, staining with PE Annexin V is typically used in conjunction with a vital dye such as

7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (7-AAD negative, PE Annexin V positive). Viable

cells with intact membranes exclude 7-AAD, wheras the membranes of dead and damaged cells are permeable to 7-AAD. For example, cells

that are considered viable are PE Annexin V and 7-AAD negative; cells that are in early apoptosis are PE Annexin V positive and 7-AAD

negative; and cells that are in late apoptosis or already dead are are both PE Annexin V and 7-AAD positive. This assay does not distinguish

between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead

cells will stain with both PE Annexin V and 7-AAD. However, when apoptosis is measured over time, cells can be often tracked from PE

Annexin V and 7-AAD negative (viable, or no measurable apoptosis), to PE Annexin V positive and 7-AAD negative (early apoptosis,

membrane integrity is present) and finally to PE Annexin V and 7-AAD positive (end stage apoptosis and death). The movement of cells

through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both PE Annexin V and 7-AAD

positive, in of itself, reveals less information about the process by which the cells underwent their demise.

559763 Rev. 8 Page 1 of 3

Flow Cytometric Analysis of PE Annexin V staining. Jurkat cells

(Human T-cell leukemia; ATCC TIB-152) were left untreated (top

panels) or treated for 4 hours with 4 μM Camptothecin (bottom

panels). Cells were incubated with PE Annexin V in a buffer

containing 7-Amino-Actinomycin (7-AAD) and analyzed by flow

cytometry. Untreated cells were primarily PE Annexin V and 7-AAD

negative, indicating that they were viable and not undergoing

apoptosis. After a 4 hour treatment (bottom panels), there were

primarily two populations of cells: Cells that were viable and not

undergoing apoptosis (PE Annexin V and 7-AAD negative); cells

undergoing apoptosis (PE Annexin V positive and 7-AAD negative).

A minor population of cells were observed to be PE Annexin V and

7-AAD positive, indicating that they were in end stage apoptosis or

already dead.

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Application Notes

Application

Flow cytometry Routinely Tested

Recommended Assay Procedure:

PE Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a

higher affinity for phosphatidylserine (PS) than most other phospholipids. PE Annexin V binding is calcium dependent and defined calcium and

salt concentrations are required for optimal staining as described in the PE Annexin V Staining Protocol. Investigators should note that PE

Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane damage

may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types, however,

have been previously reported (Casiola-Rosen et al. and van Engelend et al.).

INDUCTION OF APOPTOSIS BY CAMPTOTHECIN

The following protocol is provided as an illustration on how PE Annexin V may be used on a cell line (Jurkat).

Materials

1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO.

2. Jurkat T cells (ATCC TIB-152).

Procedure

1. Add Camptothecin (final conc. 4-6 μM) to 1 x 10^6 Jurkat cells.

2. Incubate the cells for 4-6 hr at 37°C.

3. Proceed with the PE Annexin V Staining Protocol to measure apoptosis.

PE ANNEXIN V STAINING PROTOCOL

PE Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on

the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid

phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external

environment. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying

apoptotic cells with exposed PS. 7-Amino-Actinomycin (7-AAD) is a standard flow cytometric viability probe and is used to distinguish viable

from nonviable cells. Viable cells with intact membranes exclude 7-AAD, whereas the membranes of dead and damaged cells are permeable to

7-AAD. Cells that stain positive for PE Annexin V and negative for 7-AAD are undergoing apoptosis. Cells that stain positive for both PE

Annexin V and 7-AAD are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both PE

Annexin V and 7-AAD are alive and not undergoing measurable apoptosis.

559763 Rev. 8 Page 2 of 3

Reagents

1. PE Annexin V (component no. 51-65875X): Use 5 μl per test.

2. 7-Amino-Actinomycin (7-AAD) (component no. 51-68981E) is a convenient, ready-to-use nucleic acid dye. Use 5 μl per test.

3. 10X Annexin V Binding Buffer (component no. 51-66121E): 0.1 M Hepes/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2. For a 1X working

solution, dilute 1 part of the 10X Annexin V Binding Buffer to 9 parts of distilled water.

Staining

1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml.

2. Transfer 100 μl of the solution (1 x 10^5 cells) to a 5 ml culture tube.

3. Add 5 μl of PE Annexin V and 5 μl 7-AAD.

4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.

5. Add 400 μl of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1 hr.

SUGGESTED CONTROLS FOR SETTING UP FLOW CYTOMETRY

The following controls are used to set up compensation and quadrants:

1. Unstained cells.

2. Cells stained with PE Annexin V (no 7-AAD).

3. Cells stained with 7-AAD (no PE Annexin V).

Other Staining Controls:

A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining with PE Annexin V and/or PE

Annexin V and 7-AAD. It is important to note that the basal level of apoptosis and necrosis varies considerably within a population. Thus, even in

the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (PE Annexin V

positive, 7-AAD negative or PE Annexin V positive, 7-AAD positive).

The untreated population is used to define the basal level of apoptotic and dead cells. The percentage of cells that have been induced to undergo

apoptosis is then determined by subtracting the percentage of apoptotic cells in the untreated population from percentage of apoptotic cells in the

treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged

membrane and stain positive for 7-AAD as well as for PE Annexin V. Thus the assay does not distinguish between cells that have already

undergone an apoptotic cell death and those that have died as a result of necrotic pathway, because in either case the dead cells will stain with

both PE Annexin V and 7-AAD.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental

sample (a test).

1.

2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before

discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.

4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

References

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