描述
pBI121-mCherry Plant Expression Vector
植物表达载体
产品信息：
|
货号 |
产品名称 | 规格 | 价格（元） |
| MF3723-2UG | pBI121-mCherry Plant Expression Vector 植物表达载体 | 2μg |
1200 |
基本信息
| 载体用途： | 植物双元表达载体 |
| 载体大小： | 13603bp |
| 原核抗性： | 卡那霉素（Kanamycin, Kan） |
| 克隆菌株： | DH5α |
| 荧光标记： | mCherry（红色荧光） |
| 启动子： | CaMV 35S |
| 复制子： | oriV |
| 终止子： | NOS |
| 真核抗性： | Neo |
| 表达水平： | 高 |
| 载体拷贝数： | 低 |
| 5’测序引物： | CaMV35S-f (GACGCACAATCCCACTATCC) |
| 3’测序引物： | 根据序列设计引物 |
质粒图谱
保存与运输方法
保存：-20℃保存，至少1年有效。
运输：冰袋运输。
注意事项
- 我司提供的载体/质粒/菌株都经过严格的质量控制。请收到货后先进行转化，再挑取单克隆扩增。产品使用中若遇到质量问题，请和我司及时沟通。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
质粒序列
LOCUS Exported 13603 bp ds-DNA circular SYN 31-DEC-2012
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13603)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 13, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..13603
/organism=”synthetic DNA construct”
/mol_type=”other DNA”
CDS complement(145..516)
/codon_start=1
/gene=”traJ”
/product=”oriT-recognizing protein”
/note=”traJ”
/translation=”MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP”
oriT 549..658
/note=”incP origin of transfer”
CDS complement(1339..1989)
/codon_start=1
/gene=”tetR”
/product=”tetracycline resistance regulatory protein”
/note=”TetR”
/translation=”MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD”
misc_feature 2454..2478
/note=”RB T-DNA repeat”
/note=”right border repeat from nopaline C58 T-DNA”
promoter 2602..2781
/note=”NOS promoter”
/note=”nopaline synthase promoter”
promoter 2634..2817
/function=”NOS promoter”
/note=”NOS promoter”
/note=”nopaline synthase promoter”
CDS 2838..3632
/codon_start=1
/gene=”aph(3′)-II (or nptII)”
/product=”aminoglycoside phosphotransferase from Tn5″
/note=”NeoR/KanR”
/note=”confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))”
/translation=”MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF”
terminator 4022..4274
/note=”NOS terminator”
/note=”nopaline synthase terminator and poly(A) signal”
protein_bind 4823..4844
/bound_moiety=”E. coli catabolite activator protein”
/note=”CAP binding site”
/note=”CAP binding activates transcription in the presence
of cAMP.”
promoter 4859..4889
/note=”lac promoter”
/note=”promoter for the E. coli lac operon”
protein_bind 4897..4913
/bound_moiety=”lac repressor encoded by lacI”
/note=”lac operator”
/note=”The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG).”
primer_bind 4921..4937
/note=”M13 rev”
/note=”common sequencing primer, one of multiple similar
variants”
promoter 5461..5806
/note=”CaMV 35S promoter”
/note=”strong constitutive promoter from cauliflower mosaic
virus”
CDS 5845..6555
/codon_start=1
/product=”monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)”
/note=”mCherry”
/note=”mammalian codon-optimized”
/translation=”MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK”
terminator 6572..6824
/note=”NOS terminator”
/note=”nopaline synthase terminator and poly(A) signal”
primer_bind complement(6833..6849)
/note=”M13 fwd”
/note=”common sequencing primer, one of multiple similar
variants”
misc_feature 7467..7491
/note=”LB T-DNA repeat”
/note=”left border repeat from nopaline C58 T-DNA”
CDS complement(9087..10235)
/codon_start=1
/product=”trans-acting replication protein that binds to
and activates oriV”
/note=”trfA”
/translation=”MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR”
CDS complement(10534..11328)
/codon_start=1
/gene=”aphA-3″
/product=”aminoglycoside phosphotransferase”
/note=”KanR”
/note=”confers resistance to kanamycin”
/translation=”MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF”
mobile_element 11520..12287
/mobile_element_type=”insertion sequence:IS1″
/note=”IS1″
/note=”prokaryotic transposable element”
rep_origin 12910..18
/note=”oriV”
/note=”incP origin of replication”
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
5821 ggatccccgg gtggtcagtc ccttatggtg agcaagggcg aggaggataa catggccatc
5881 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
5941 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
6001 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
6061 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
6121 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
6181 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
6241 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
6301 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
6361 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
6421 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
6481 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
6541 gagctgtaca agtgagagct cgaatttccc cgatcgttca aacatttggc aataaagttt
6601 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
6661 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
6721 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
6781 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tcactggccg
6841 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
6901 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
6961 aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc
7021 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
7081 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
7141 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
7201 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
7261 taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa
7321 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
7381 tgcccgtctc actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg
7441 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
7501 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
7561 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
7621 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
7681 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc
7741 cctcgcagag atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct
7801 gtcgatcttg agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag
7861 ctgagtggcg ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc
7921 tcaccgaatg gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg
7981 ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag
8041 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca
8101 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc
8161 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg
8221 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc
8281 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc
8341 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag
8401 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac
8461 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag
8521 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat
8581 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg
8641 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc
8701 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac
8761 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac
8821 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg
8881 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc
8941 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa
9001 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc
9061 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
9121 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
9181 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
9241 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
9301 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
9361 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
9421 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
9481 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
9541 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
9601 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
9661 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
9721 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
9781 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
9841 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
9901 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
9961 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg
10021 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
10081 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
10141 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
10201 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
10261 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
10321 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
10381 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
10441 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
10501 ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata
10561 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
10621 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
10681 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
10741 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
10801 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
10861 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
10921 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
10981 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
11041 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
11101 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
11161 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
11221 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
11281 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
11341 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
11401 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
11461 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg
11521 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
11581 tctgtgtcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
11641 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
11701 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
11761 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
11821 cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca
11881 tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct
11941 ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac
12001 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat
12061 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca
12121 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac
12181 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
12241 ggcattatct gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga
12301 atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat
12361 gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat
12421 ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata
12481 tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa
12541 tatggcgctt catagagtaa ttctgtaaag gtccaattct cgttttcata cctcggtata
12601 atcttaccta tcacctcaaa tggttcgctg ggtttatcgc acccccgaac acgagcacgg
12661 cacccgcgac cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt
12721 ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac
12781 tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg
12841 gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga
12901 ctgccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
12961 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
13021 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
13081 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
13141 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
13201 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
13261 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
13321 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
13381 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa
13441 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
13501 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg
13561 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cgg