产品名称
HRP偶联试剂盒?- Lightning-Link?
产品概述
HRP Conjugation Kit / HRP Labeling Kit ab102890 uses a simple and quick process for?HRP?labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides.?Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to HRP using this kit:
– add modifier to antibody and incubate for 3 hrs
– add quencher and incubate for 30 mins
The HRP conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to HRP.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
说明
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link? HRP Labeling Kit. 701-0004 is the same as the 5 mg size. 701-0003 is the same as the 5 x 1 mg size. 701-0000 is the same as the 3 x 100 ug size. 701-0030 is the same as the 3 x 10 ug size. 701-0010 is the same as the 100 μg size. 701-0002 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to HRP
Kit size
Recommended
amount of antibody1
Maximum
amount of antibody
Maximum antibody
volume2
3 x 10 μg
3 x 10 μg
3 x?40 μg
3 x 10 μL
100 μg
1 x 100 μg
1 x 400 μg
1 x 100 μL
3 x 100 μg
3 x 100 μg
3 x 400 μg
3 x 100 μL
1 mg
1 x 1 mg
1 x 4 mg
1 x 1 mL
5 x 1 mg
5 x 1 mg
5 x 4 mg
5 x 1 mL
5 mg
1 x?5 mg
1 x 20 mg
1 x 5 mL
1 Recommended amount of antibody will give an HRP:antibody molar ratio of 4:1. Antibodies often also perform well at the maximum amount of antibody which is 1:1 molar ratio.
2 Ideal?antibody concentration is 1mg/ml. 0.5 – 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 4 mg/ml or < 0.5 mg/ml should be diluted /concentrated. Buffer Requirements for Conjugation Buffer should be?pH 6.5-8.5. Buffer constituent compatibility If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation. Compatible buffer constituents 50mM / 0.6% Tris1 0.1% BSA 50% glycerol 0.1% sodium azide2 PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose 1 Tris buffered saline is almost always ≤ 50 mM / 0.6% 2 Sodium azide irreversibly inhibits HRP; antibodies with azide should be purified before using the HRP conjugation kit. Incompatible buffer constituents Thiomerosal Proclin Glycine Arginine Glutathione DTT If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us. Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids?are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible. Storing and handling conjugation kits Lyophilized Lightning-Link? components are hygroscopic. Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation. 性能 存放说明 Store at -20°C. Please refer to protocols. 组件 100 μg 1 mg 3 x 10 μg 5 x 1 mg 3 x 100 μg 1 x 5 mg ab274112 - HRP mix 1 x 100μg 1 x 1mg 3 x 10μg 5 x 1mg 3 x 100μg 1 x 5mg ab274106 - Modifier reagent 1 x 200μl 1 x 200μl 1 x 200μl 1 x 1200μl 1 x 200μl 1 x 1200μl ab274296 - Quencher reagent 1 x 200μl 1 x 200μl 1 x 200μl 1 x 1200μl 1 x 200μl 1 x 1200μl
