Hexidium Iodide (HI) 碘化乙啶(核酸染色剂)CAS NO:211566-66-4

Hexidium Iodide (HI) 碘化乙啶;Phenanthridinium dye 菲啶鎓染料;核酸染色剂;Ethidium bromide (EtBr) 溴化乙啶;Propidium iodide (PI) 碘化丙啶;CAS NO:211566-66-4;

产品信息

产品名称

产品编号              规格   CAS NO.   
Hexidium Iodide (HI) 碘化乙啶    MX4233-5MG 5mg 211566-66-4

产品描述

碘化乙啶(Hexidium Iodide, HI)是一种中等亲脂的菲啶鎓染料,一种核酸染色剂,具哺乳动物细胞膜通透性,在含革兰氏阴性菌的环境下选择性染色几乎所有的革兰氏阳性菌。一旦与DNA结合,碘化乙啶发出最大激发/发射波长分别约为518/600nm的荧光。通常,真核细胞的细胞质和细胞核都会被碘化乙啶染色。然而,线粒体和核仁也可能被染色。

产品特性

1)   CAS NO.:211566-66-4

2)   化学名:Phenanthridinium, 3,8-diamino-5-hexyl-6-phenyl-, iodide

3)   分子式:C25H28IN3

4)   分子量:497.42

5)   外观:固体

6)   纯度:≥95%

7)   溶解性:溶于DMSO

8)   Ex/Em:~518⁄600nm(与DNA结合)

9)   化学结构式:

保存与运输方法

保存:-20°C避光干燥保存,至少1年有效。

运输:冰袋运输。

注意事项

1)荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

染色示例(来自文献,仅作参考)

文献1)Yan Q, Ahn SH, Fowler VG Jr. Macrophage Phagocytosis Assay of <i>Staphylococcus aureus</i> by Flow Cytometry. Bio-protocol. 2015 Feb;5(4). PMID: 25879046; PMCID: PMC4394738.

实验目的:用碘化乙啶(HI)染色金黄色葡萄球菌(S. aureus),从而评估小鼠巨噬细胞对金黄色葡萄球菌的吞噬能力。

实验方法:a)50ml离心管内装1ml S. aureus(5 × 108/ml);b)加入20 μl碘化乙啶储存液(浓度为:5mg/ml,用1ml DMSO加入5mg HI粉末,充分溶解后,分装避光冻存在-20℃),混匀;c)室温避光孵育15min;4)冰DPBS清洗2次(4 °C, 3,000 rpm, 10 min);5)用BMM培养基重悬细菌,使其密度为2 × 107/ml,此时的细菌立即进行后续的分析;6)用FACSCanto流式细胞仪进行检测,碘化乙啶的荧光用FL2通道(Ex/Em= 488/575nm)来搜集信号。用FlowJo进行结果分析。

Figure 1 Practical example of a bone marrow-derived macrophage (BMDM) phagocytosis experiment

Blank: BMDMs without both FITC-anti-F4/80 staining and bacterial uptake. FITC: BMDMs with FITC-anti-F4/80 staining but without bacterial uptake. Hexidium Iodide: BMDMs with bacterial uptake but without FITC-anti-F4/80 staining. BMDM-phagocytosis: BMDMs with both FITC-anti-F4/80 staining and bacterial uptake.

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MF0756-5ML EB (10 mg/ml in Water) EB水溶液(10 mg/ml) 5ml
MX4205-10MG Propidium Iodide碘化丙啶(粉末) 10mg
MX4205-1ML Propidium Iodide (1mg/ml)碘化丙啶(1mg/ml) 1ml
MX4213-1MG Ethidium Homodimer 1 (EthD-1)溴乙啡锭二聚体1 1mg
MX4214-1MG Ethidium Homodimer 3 (EthD-3)溴乙啡锭二聚体3 1mg
MX4215-1MG 7-AAD (7-Aminoactinomycin D) 7-氨基放线菌素D 1mg
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MX4220-1MG Propidium Monoazide (PMA)叠氮溴化丙锭 1mg
MX4212-5MG Ethidium Monoazide Bromide (EMA)叠氮溴化乙锭 5mg
MX4213-1MG Ethidium Homodimer 1 (EthD-1)溴乙啡锭二聚体1 1mg         
MX4214-1MG Ethidium Homodimer 3 (EthD-3)溴乙啡锭二聚体3 1mg

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)


描述

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

 

产品标签

MPP+ iodide;MPTP;帕金森病动物模型;Ferroptosisinhibitor;Necrostatin-1;Ferrostatin-1 (Fer-1);CAS:36913-39-0;

产品信息: 

产品名称

产品编号 CAS NO. 规格 价格(元)

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

MZ6402-50MG 36913-39-0 50mg 922
MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-100MG 36913-39-0 100mg

1592

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-250MG 36913-39-0 250mg

3522

产品描述

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)是神经毒素MPTP的活性代谢物,在帕金森病动物模型中选择性破坏多巴胺能神经元。MPP+主要通过抑制线粒体电子传递链复合物I,引起ATP耗竭和氧化应激增高,从而诱导神经毒性。MPP+诱导Necrostatin-1 (Nec-1)和 Ferrostatin-1 (Fer-1)敏感的SH-SY5Y细胞坏死,但MPP+诱导的坏死性细胞死亡与铁坏死是不同的生理过程。

产品特性

1) 化学名:1-Methyl-4-phenylpyridin-1-ium iodide

2) 同义名:N-Methyl-4-Phenylpyridinium Iodide; 1-methyl-4-phenyl-pyridinium, monoiodide; MPP+;N-甲基-4-苯基吡啶鎓碘化物;1-甲基-4-苯基-吡啶鎓碘化物;1-甲基-4-苯基吡啶碘化物;

3) CAS NO:36913-39-0

4) 分子式:C12H12IN

5) 分子量:297.1

6) 纯度:≥98%

7) 外观:固体

8) 溶解性:溶于H2O(100mM)

9) 化学结构式:

保存与运输方法

保存:-20ºC避光干燥保存,至少2年有效。 

运输:冰袋运输。

注意事项

1) 关于化合物溶解性:产品特性内的“≥”表明溶于标示浓度,但饱和溶解度未知。不同批次化合物的溶解度会有差异。

2) 为了让化合物更好的溶解,可通过37℃加热或(和)超声波水浴中震动片刻来处理。若实验所需浓度过大甚至达产品溶解极限,请添加助溶剂助溶或自行调整浓度。

3) 本品仅用作科研用途,不可用于临床或诊疗用途。

4) 为了您的安全和健康,请穿实验服并戴一次性手套操作。

储存液制备

          质量

溶剂体积

浓度

1mg 5mg 10mg
1mM 3.3654 mL 16.8271 mL 33.6542mL
5mM 0.6731mL 3.3654 mL 6.7308mL
10mM 0.3365 mL 1.6827 mL 3.3654 mL

【温馨提示】:请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;MPP+在溶液中不稳定,建议新鲜配制溶液且立即使用。配制好的溶液需避光保存和使用。

使用方法【源自文献,仅作参考】

文献1,Stahl K, Rahmani S, Prydz A, Skauli N, MacAulay N, Mylonakou MN, Torp R, Skare Ø, Berg T, Leergaard TB, Paulsen RE, Ottersen OP, Amiry-Moghaddam M. Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson’s disease. PLoS One. 2018 Mar 22;13(3):e0194896. doi: 10.1371/journal.pone.0194896. PMID: 29566083; PMCID: PMC5864064.

体内研究(动物模型):

动物模型(Animal Model):C57BL/6J WT and Aqp9-/- mice

配制方法(Formulation):MPP+ (7.5 μg dissolved in saline)

实验方法(Assay):C57BL/6J WT (n = 34) and Aqp9-/- mice (n = 29) were deeply anesthetized with zoletil mixture, Rompunand Fentanyl and then subjected tostereotaxic intrastriatal injections of MPP+ (7.5 μg dissolved in saline) or saline. 1 μl MPP+ solution or saline was injected into the striatum 0.6 mm anterior to Bregma, 2.2 mm laterally and 3.2 mm ventrally at 12 μl/hr using a syringe pump. MPP+ was protected against light during the procedure. The injector was left for 5 minutes to allow diffusion before suture.

文献2,Xu J, Gao X, Yang C, Chen L, Chen Z. Resolvin D1 Attenuates Mpp+-Induced Parkinson Disease via Inhibiting Inflammation in PC12 Cells. Med Sci Monit. 2017 Jun 2;23:2684-2691. doi: 10.12659/msm.901995. PMID: 28572562; PMCID: PMC5465971.

体外研究(细胞实验):

细胞类型(Cell types):PC12 cells

实验方法(Assay):The in vitro PD model was established by treatment of PC12 cells with250 μM MPP+ for approximately 24 hours.Different concentrations of RvD1 (50, 100, 200 nM) were administrated two hours prior to MPP+ treatment, afterwards, PC12 cells were used for following experiments.

文献3,Christensen C, Þorsteinsson H, Maier VH, Karlsson KÆ. Multi-parameter Behavioral Phenotyping of the MPP+ Model of Parkinson’s Disease in Zebrafish. Front Behav Neurosci. 2020 Dec 18;14:623924. doi: 10.3389/fnbeh.2020.623924. PMID: 33390914; PMCID: PMC7775599.

体内研究(动物模型):

动物模型(Animal Model):Zebrafish larvae

配制方法(Formulation):Both MPP+ and PBA were dissolved in the system water.

实验方法(Assay):Zebrafish larvae were incubated between 1 and 5 dpf in the presence or absence of 500 μM MPP+ at a total volume of 25 ml system water. The drug solutions were freshly prepared and replaced between 11:00 and 12:00 am at 2, 3, and 4 dpf.Deceased larvae were removed daily, but no differences in mortality were observed between MPP+ exposed larvae and untreated larvae.

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MZ5272-5MG Erastin铁死亡激活剂 5mg
MZ6402-50MG MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide) 50mg
MZ6403-25MG MPTP hydrochloride 25mg
MX4558-50UG FeRhoNox-1 (Fe2+ indicator)亚铁离子荧光探针 50μg
MX4559-24UG FerroOrange (Fe2+ indicator)亚铁离子荧光探针 24μg
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MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)


描述

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

 

产品标签

MPP+ iodide;MPTP;帕金森病动物模型;Ferroptosisinhibitor;Necrostatin-1;Ferrostatin-1 (Fer-1);CAS:36913-39-0;

产品信息: 

产品名称

产品编号 CAS NO. 规格 价格(元)

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

MZ6402-50MG 36913-39-0 50mg 922
MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-100MG 36913-39-0 100mg

1592

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-250MG 36913-39-0 250mg

3522

产品描述

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)是神经毒素MPTP的活性代谢物,在帕金森病动物模型中选择性破坏多巴胺能神经元。MPP+主要通过抑制线粒体电子传递链复合物I,引起ATP耗竭和氧化应激增高,从而诱导神经毒性。MPP+诱导Necrostatin-1 (Nec-1)和 Ferrostatin-1 (Fer-1)敏感的SH-SY5Y细胞坏死,但MPP+诱导的坏死性细胞死亡与铁坏死是不同的生理过程。

产品特性

1) 化学名:1-Methyl-4-phenylpyridin-1-ium iodide

2) 同义名:N-Methyl-4-Phenylpyridinium Iodide; 1-methyl-4-phenyl-pyridinium, monoiodide; MPP+;N-甲基-4-苯基吡啶鎓碘化物;1-甲基-4-苯基-吡啶鎓碘化物;1-甲基-4-苯基吡啶碘化物;

3) CAS NO:36913-39-0

4) 分子式:C12H12IN

5) 分子量:297.1

6) 纯度:≥98%

7) 外观:固体

8) 溶解性:溶于H2O(100mM)

9) 化学结构式:

保存与运输方法

保存:-20ºC避光干燥保存,至少2年有效。 

运输:冰袋运输。

注意事项

1) 关于化合物溶解性:产品特性内的“≥”表明溶于标示浓度,但饱和溶解度未知。不同批次化合物的溶解度会有差异。

2) 为了让化合物更好的溶解,可通过37℃加热或(和)超声波水浴中震动片刻来处理。若实验所需浓度过大甚至达产品溶解极限,请添加助溶剂助溶或自行调整浓度。

3) 本品仅用作科研用途,不可用于临床或诊疗用途。

4) 为了您的安全和健康,请穿实验服并戴一次性手套操作。

储存液制备

          质量

溶剂体积

浓度

1mg 5mg 10mg
1mM 3.3654 mL 16.8271 mL 33.6542mL
5mM 0.6731mL 3.3654 mL 6.7308mL
10mM 0.3365 mL 1.6827 mL 3.3654 mL

【温馨提示】:请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;MPP+在溶液中不稳定,建议新鲜配制溶液且立即使用。配制好的溶液需避光保存和使用。

使用方法【源自文献,仅作参考】

文献1,Stahl K, Rahmani S, Prydz A, Skauli N, MacAulay N, Mylonakou MN, Torp R, Skare Ø, Berg T, Leergaard TB, Paulsen RE, Ottersen OP, Amiry-Moghaddam M. Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson’s disease. PLoS One. 2018 Mar 22;13(3):e0194896. doi: 10.1371/journal.pone.0194896. PMID: 29566083; PMCID: PMC5864064.

体内研究(动物模型):

动物模型(Animal Model):C57BL/6J WT and Aqp9-/- mice

配制方法(Formulation):MPP+ (7.5 μg dissolved in saline)

实验方法(Assay):C57BL/6J WT (n = 34) and Aqp9-/- mice (n = 29) were deeply anesthetized with zoletil mixture, Rompunand Fentanyl and then subjected tostereotaxic intrastriatal injections of MPP+ (7.5 μg dissolved in saline) or saline. 1 μl MPP+ solution or saline was injected into the striatum 0.6 mm anterior to Bregma, 2.2 mm laterally and 3.2 mm ventrally at 12 μl/hr using a syringe pump. MPP+ was protected against light during the procedure. The injector was left for 5 minutes to allow diffusion before suture.

文献2,Xu J, Gao X, Yang C, Chen L, Chen Z. Resolvin D1 Attenuates Mpp+-Induced Parkinson Disease via Inhibiting Inflammation in PC12 Cells. Med Sci Monit. 2017 Jun 2;23:2684-2691. doi: 10.12659/msm.901995. PMID: 28572562; PMCID: PMC5465971.

体外研究(细胞实验):

细胞类型(Cell types):PC12 cells

实验方法(Assay):The in vitro PD model was established by treatment of PC12 cells with250 μM MPP+ for approximately 24 hours.Different concentrations of RvD1 (50, 100, 200 nM) were administrated two hours prior to MPP+ treatment, afterwards, PC12 cells were used for following experiments.

文献3,Christensen C, Þorsteinsson H, Maier VH, Karlsson KÆ. Multi-parameter Behavioral Phenotyping of the MPP+ Model of Parkinson’s Disease in Zebrafish. Front Behav Neurosci. 2020 Dec 18;14:623924. doi: 10.3389/fnbeh.2020.623924. PMID: 33390914; PMCID: PMC7775599.

体内研究(动物模型):

动物模型(Animal Model):Zebrafish larvae

配制方法(Formulation):Both MPP+ and PBA were dissolved in the system water.

实验方法(Assay):Zebrafish larvae were incubated between 1 and 5 dpf in the presence or absence of 500 μM MPP+ at a total volume of 25 ml system water. The drug solutions were freshly prepared and replaced between 11:00 and 12:00 am at 2, 3, and 4 dpf.Deceased larvae were removed daily, but no differences in mortality were observed between MPP+ exposed larvae and untreated larvae.

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MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)


描述

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

 

产品标签

MPP+ iodide;MPTP;帕金森病动物模型;Ferroptosisinhibitor;Necrostatin-1;Ferrostatin-1 (Fer-1);CAS:36913-39-0;

产品信息: 

产品名称

产品编号 CAS NO. 规格 价格(元)

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

MZ6402-50MG 36913-39-0 50mg 922
MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-100MG 36913-39-0 100mg

1592

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-250MG 36913-39-0 250mg

3522

产品描述

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)是神经毒素MPTP的活性代谢物,在帕金森病动物模型中选择性破坏多巴胺能神经元。MPP+主要通过抑制线粒体电子传递链复合物I,引起ATP耗竭和氧化应激增高,从而诱导神经毒性。MPP+诱导Necrostatin-1 (Nec-1)和 Ferrostatin-1 (Fer-1)敏感的SH-SY5Y细胞坏死,但MPP+诱导的坏死性细胞死亡与铁坏死是不同的生理过程。

产品特性

1) 化学名:1-Methyl-4-phenylpyridin-1-ium iodide

2) 同义名:N-Methyl-4-Phenylpyridinium Iodide; 1-methyl-4-phenyl-pyridinium, monoiodide; MPP+;N-甲基-4-苯基吡啶鎓碘化物;1-甲基-4-苯基-吡啶鎓碘化物;1-甲基-4-苯基吡啶碘化物;

3) CAS NO:36913-39-0

4) 分子式:C12H12IN

5) 分子量:297.1

6) 纯度:≥98%

7) 外观:固体

8) 溶解性:溶于H2O(100mM)

9) 化学结构式:

保存与运输方法

保存:-20ºC避光干燥保存,至少2年有效。 

运输:冰袋运输。

注意事项

1) 关于化合物溶解性:产品特性内的“≥”表明溶于标示浓度,但饱和溶解度未知。不同批次化合物的溶解度会有差异。

2) 为了让化合物更好的溶解,可通过37℃加热或(和)超声波水浴中震动片刻来处理。若实验所需浓度过大甚至达产品溶解极限,请添加助溶剂助溶或自行调整浓度。

3) 本品仅用作科研用途,不可用于临床或诊疗用途。

4) 为了您的安全和健康,请穿实验服并戴一次性手套操作。

储存液制备

          质量

溶剂体积

浓度

1mg 5mg 10mg
1mM 3.3654 mL 16.8271 mL 33.6542mL
5mM 0.6731mL 3.3654 mL 6.7308mL
10mM 0.3365 mL 1.6827 mL 3.3654 mL

【温馨提示】:请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;MPP+在溶液中不稳定,建议新鲜配制溶液且立即使用。配制好的溶液需避光保存和使用。

使用方法【源自文献,仅作参考】

文献1,Stahl K, Rahmani S, Prydz A, Skauli N, MacAulay N, Mylonakou MN, Torp R, Skare Ø, Berg T, Leergaard TB, Paulsen RE, Ottersen OP, Amiry-Moghaddam M. Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson’s disease. PLoS One. 2018 Mar 22;13(3):e0194896. doi: 10.1371/journal.pone.0194896. PMID: 29566083; PMCID: PMC5864064.

体内研究(动物模型):

动物模型(Animal Model):C57BL/6J WT and Aqp9-/- mice

配制方法(Formulation):MPP+ (7.5 μg dissolved in saline)

实验方法(Assay):C57BL/6J WT (n = 34) and Aqp9-/- mice (n = 29) were deeply anesthetized with zoletil mixture, Rompunand Fentanyl and then subjected tostereotaxic intrastriatal injections of MPP+ (7.5 μg dissolved in saline) or saline. 1 μl MPP+ solution or saline was injected into the striatum 0.6 mm anterior to Bregma, 2.2 mm laterally and 3.2 mm ventrally at 12 μl/hr using a syringe pump. MPP+ was protected against light during the procedure. The injector was left for 5 minutes to allow diffusion before suture.

文献2,Xu J, Gao X, Yang C, Chen L, Chen Z. Resolvin D1 Attenuates Mpp+-Induced Parkinson Disease via Inhibiting Inflammation in PC12 Cells. Med Sci Monit. 2017 Jun 2;23:2684-2691. doi: 10.12659/msm.901995. PMID: 28572562; PMCID: PMC5465971.

体外研究(细胞实验):

细胞类型(Cell types):PC12 cells

实验方法(Assay):The in vitro PD model was established by treatment of PC12 cells with250 μM MPP+ for approximately 24 hours.Different concentrations of RvD1 (50, 100, 200 nM) were administrated two hours prior to MPP+ treatment, afterwards, PC12 cells were used for following experiments.

文献3,Christensen C, Þorsteinsson H, Maier VH, Karlsson KÆ. Multi-parameter Behavioral Phenotyping of the MPP+ Model of Parkinson’s Disease in Zebrafish. Front Behav Neurosci. 2020 Dec 18;14:623924. doi: 10.3389/fnbeh.2020.623924. PMID: 33390914; PMCID: PMC7775599.

体内研究(动物模型):

动物模型(Animal Model):Zebrafish larvae

配制方法(Formulation):Both MPP+ and PBA were dissolved in the system water.

实验方法(Assay):Zebrafish larvae were incubated between 1 and 5 dpf in the presence or absence of 500 μM MPP+ at a total volume of 25 ml system water. The drug solutions were freshly prepared and replaced between 11:00 and 12:00 am at 2, 3, and 4 dpf.Deceased larvae were removed daily, but no differences in mortality were observed between MPP+ exposed larvae and untreated larvae.

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PI [Propidium iodide], 25535-16-4

Propidium Iodide(碘化丙啶,PI)是一种核酸荧光染料,可用于哺乳动物、细菌、酵母的染色。它不能透过完整的细胞膜,但能够透过死细胞和凋亡中晚期细胞的细胞膜而使细胞核染色。因此,PI 作为荧光探针常用于细胞凋亡(apoptosis)或细胞坏死(necrosis)的检测。进入细胞后,PI 与 DNA 和 RNA 结合,荧光强度增强 20-30 倍。

产品名称 PI [Propidium iodide], 25535-16-4
目录号 910802
中文名称 碘化丙啶
英文名称 Propidium iodide
CAS 25535-16-4
分子式 C27H34I2N4
分子量 668.39
存储条件 -20°干燥避光
保存时间 一年
Ex/Em(nm) 535/617

DiR iodide,100068-60-8

DiR 是一个亲脂性、近红外荧光花青染料。这个染料常用于标记细胞质膜。DiR 的两个 18-碳链插入到细胞膜,从而进行特定的、稳定的细胞染色,几乎不会发生细胞间的染料转移。

产品名称 DiR iodide,100068-60-8
目录号 910814
中文名称 DiR 碘化物
英文名称 DiR iodide
CAS 100068-60-8
分子式 C63H101N2I
分子量 1013.39
存储条件 -20°干燥避光
保存时间 一年
Ex/Em(nm) 748/780