临床前研究用检测试剂 免疫·过敏研究领域

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临床前研究用检测试剂
免疫·过敏研究领域

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临床前研究用检测试剂                              免疫·过敏研究领域




免疫球蛋白E(IgE)

生产商编号
和光编号
产品名称 检测范围

检测样本量

(稀释样本)

AKRIE-010

633-02899

LBIS® IgE Mouse ELISA Kit  
小鼠免疫球蛋白E(IgE) ELISA试剂盒 
1~100 ng/mL 50 μL
AKRIE-011
636-04349

LBIS® IgE Rat ELISA Kit  
大鼠免疫球蛋白E(IgE)ELISA试剂盒

1~100 ng/mL 50 μL


◆白细胞介素(Interleukin)

生产商编号

和光编号

产品名称 检测范围 检测样本量
(稀释样本)
AKMIL12-011
638-40841
LBIS® Mouse IL-12 ELISA   Kit
小鼠白细胞介素-12(IL-12)ELISA试剂盒
2.87~700 pg/mL 100 μL

TDAR(T cell Dependent Antibody Responce)试验用


生产商编号
和光编号
产品名称 检测范围 检测样本量
(稀释样本)
AKRKG-010
632-13751

LBIS® KLH(TDAR)-Rat IgG ELISA KIT
血蓝蛋白(KLH)(T细胞依赖性抗原)

大鼠免疫球蛋白G(IgG) ELISA试剂盒 (*1)

0.47~30 ng/mL 50 μL
AKRKM-010
639-13761

LBIS® KLH(TDAR)-Rat IgM ELISA KIT
血蓝蛋白(KLH)(T细胞依赖性抗原)

大鼠免疫球蛋白M(IgM) ELISA试剂盒 (*2)

3.13~200 ng/mL 50 μL


(*1) 为了避免非特异性反应,请将检测样本稀释500倍以上

(*2) 为了避免非特异性反应,请将检测样本稀释200倍以上




抗OVA(ovalbumin)抗体

生产商编号
和光编号
产品名称 检测范围 检测样本量
(稀释样本)
AKRIE-030
639-07651

LBIS® OVA-IgE Mouse
小鼠卵清蛋白特异性免疫球蛋白E

OVA-IgE)ELISA试剂盒 

1.88~120 U/mL 10 μL
AKRIE-040
636-07661

LBIS® OVA-IgG1 Mouse
小鼠卵清蛋白特异性免疫球蛋白G1

OVA-IgG1)ELISA试剂盒

1.88~120 U/mL 10 μL




◆抗核酸抗体


生产商编号
和光编号
产品名称 检测范围 检测样本量
(稀释样本)
AKRDD-061
637-02691
LBIS® Anti dsDNA-Mouse ELISA Kit
小鼠抗双链DNA抗体 ELISA试剂盒
15.6~1000mU/mL 100 μL

AKRSD-051
630-02701

LBIS® Anti ssDNA-Mouse ELISA Kit
LBIS® 抗ssDNA-小鼠ELISA试剂盒 
15.6~1000mU/mL 100 μL

类风湿因子


生产商编号
和光编号
产品名称 检测范围 检测样本量
(稀释样本)
AKRRG-101
637-02679

LBIS® Rheumatoid Factor IgG-Mouse ELISA Kit
小鼠免疫球蛋白G(IgG)

类风湿因子 ELISA试剂盒

15.6~1000 mU/mL 100 μL
AKRRG-111
634-02689

LBIS® Rheumatoid Factor IgM-Mouse ELISA Kit
小鼠免疫球蛋白M(IgM)

类风湿因子 ELISA试剂盒

15.6~1000 mU/mL 100 μL

※ 本页面产品仅供研究用,研究以外不可使用。


参考文献

◆LBIS® 小鼠免疫球蛋白E(IgE) ELISA试剂盒相关参考文献


 1.

Inhibitory Effect of Carnosol on Phthalic Anhydride-Induced Atopic Dermatitis via Inhibition of STAT3. Lee DY, Hwang CJ, Choi JY, Park MH, Song MJ, Oh KW, Son DJ, Lee SH, Han SB, Hong JT. Biomol Ther (Seoul). 2017 Sep 1;25(5):535-544.


 2.

Effects of aqueous extract from red Liriope platyphylla on phthalic-anhydride-induced atopic dermatitis in Interleukin-4/Luciferase/Consensus non-coding sequence-1 transgenic mice evaluated in terms of luciferase signal and general phenotype biomarkers. Kwak MH, Kim JE, Go J, Son HJ, Lee HS, Hong JT, Hwang DY. Journal of Traditional Chinese Medicine, Vol.37, Issue 4, Aug 2017, p475-485


 3.

Oral administration of freeze-dried powders of honey bee larvae inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice. Kageyama M, Xing G, Li K, Zhang Z, Sugiyama A. Personalized Medicine Universe, Vol. 6, July 2017, Pages 22-27.


 4.

Differential Nitric Oxide-Derived Protein Modifications of Flavin-Containing Monooxygenases in Type 1 Allergic Mice. Tanino T, Bando T, Komada A, Nojiri Y, Okada Y, Ueda Y, and Sakurai E. Drug Metabolism and Disposition  July 31, 2017,  dmd.117.076570;


 5.

Simultaneously increased expression of glucocorticoid‑induced tumor necrosis factor receptor and its ligand contributes to increased interleukin‑5/13‑producing group 2 innate lymphocytes in murine asthma. Zhang M, Wan J, Xu Y, Zhang D, Peng J, Qi C, Guo Q, Xia S, Su Z, Wang S, Xu H. Mol Med Rep. 2017 Jun;15(6):4291-4299.


 6.

Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT). Kaneko R, Sato A, Hamada S, Yagi T, Ohsawa I, Ohtsuki M, Kobayashi E, Hirabayashi M, Murakami T. Transgenic Res. 2016 Aug;25(4):413-24.


 7.

Effects of sublingual immunotherapy in a murine asthma model sensitized by intranasal administration of house dust mite extracts. Shima K, Koya T, Tsukioka K, Sakagami T, Hasegawa T, Fukano C, Ohashi-Doi K, Watanabe S, Suzuki E, Kikuchi T. Allergol Int. 2016 Jul 8.


 8.

Therapeutic effect of tyndallized Lactobacillus rhamnosus IDCC 3201 on atopic dermatitis mediated by down-regulation of immunoglobulin E in NC/Nga mice. Lee SH, Yoon JM, Kim YH, Jeong DG, Park S, Kang DJ. Microbiol Immunol. 2016 Jul;60(7):468-76.


 9.

Gain-switched 311-nm Ti:Sapphire laser might be a potential treatment modality for atopic dermatitis.. Choi SY, Oh CT, Kwon TR, Kwon HJ, Choi EJ, Jang YJ, Kim HS, Chu H, Mun SK, Kim MN, Kim BJ. Lasers Med Sci. 2016 Jul 9.


10.

Perilla leaf extract prevents atopic dermatitis induced by an extract of Dermatophagoides farinae in NC/Nga mice. Komatsu KI, Takanari J, Maeda T, Kitadate K, Sato T, Mihara Y, Uehara K, Wakame K. Asian Pac J Allergy Immunol. 2016 Mar 20.


11.

Functional polysaccharides from Grifola frondosa aqueous extract inhibit atopic dermatitis-like skin lesions in NC/Nga mice. Park HS, Hwang YH, Kim MK, Hong GE, Lee HJ, Nagappan A, Yumnam S, Kim EH, Heo JD, Lee SJ, Won CK, Kim GS. Biosci Biotechnol Biochem. Vol.79(1), p147-54, 2015.


12.

Inhibitory effect of 5,6-dihydroergosteol-glucoside on atopic dermatitis-like skin lesions via suppression of NF-κB and STAT activation. Jung M, Lee TH, Oh HJ, Kim H, Son Y, Lee EH, Kim J. J Dermatol Sci. 2015


13.

Immunopotentiator from Pantoea agglomerans Prevents Atopic Dermatitis Induced by Dermatophagoides farinae Extract in NC/Nga Mouse. Wakame K, Komatsu K, Inagawa H, Nishizawa T. Anticancer Res. Vol.35(8), p4501-8, Aug 2015.


14.

Solanum tuberosum L. cv Jayoung Epidermis Extract Inhibits Mite Antigen-Induced Atopic Dermatitis in NC/Nga Mice by Regulating the Th1/Th2 Balance and Expression of Filaggrin. Yang G, Cheon SY, Chung KS, Lee SJ, Hong CH, Lee KT, Jang DS, Jeong JC, Kwon OK, Nam JH, An HJ. J Med Food. 2015 Jun 23.


15.

Gamisasangja-tang suppresses pruritus and atopic skin inflammation in the NC/Nga murine model of atopic dermatitis. Park BK, Park YC, Jung IC, Kim SH, Choi JJ, Do M, Kim SY, Jin M. J Ethnopharmacol. 2015 Feb 23.


16.

Bacillus subtilis KCTC 11782BP-produced alginate oligosaccharide effectively suppresses asthma via T-helper cell type 2-related cytokines. Bang MA, Seo JH, Seo JW, Jo GH, Jung SK, Yu R, Park DH, Park SJ. PLoS One. 2015 Feb 6;10(2):e0117524.


17.

Effect of the hand antiseptic agents benzalkonium chloride, povidone-iodine, ethanol, and chlorhexidine gluconate on atopic dermatitis in NC/Nga mice. Sadakane K, Ichinose T. Int J Med Sci. Vol.5;12(2), p116-25, Jan 2015.


18.

Effects of oral administration of di-(2-ethylhexyl) and diisononyl phthalates on atopic dermatitis in NC/Nga mice. Sadakane K., Ichinose T., Takano H., Yanagisawa R. and Koike E. Immunopharmacology and Immunotoxicology, Vol.36(1), p61-69, Feb 2014.


19.

Oral Administration of SSC201, a Medicinal Herbal Formula, Suppresses Atopic Dermatitis-Like Skin Lesions. Kyung P., Chun P., Chul J., Hyung K., Eun C., Sunyoung P., June C. and Mirim J. Journal of Medicinal Food, 2014.


20.

Effect of Allergen Sensitization on External Root Resorption. Murata N., Ioi H., Ouchi M., Takao T., Oida H., Aijima R., Yamaza T., Kido MA. JDR, Vol.92(7), p641-647, Jul 2013.


21.

Quantitative evaluation of therapeutic effect of Liriope platyphylla on phthalic anhydride-induced atopic dermatitis in IL-4/Luc/CNS-1 Tg mice. M.H.Kwak, J.E.Kim, I.S.Hwang, Y.J.Lee, B.S.An, J.T.Hong, S.H.Lee, D.Y.Hwang. Journal of Ethnopharmacology, Vol.148(3), p880-889, Jul 2013.

22.

Lack of both α2-antiplasmin and plasminogen activator inhibitor type-1 induces high IgE production. K.Okada, S.Ueshima, N.Kawao, M.Yano, Y.Tamura, M.Tanaka, A.Sakamoto, M.Hatano, M.Arima, S.Miyata, N.Nagai, T.Tokuhisa, O.Matsuo. Life Sciences, Vol.93(2-3), p89-95, Jul 2013.

23.

Therapeutic effects of fermented soycrud on phenotypes of atopic dermatitis induced by phthalic anhydride. Sung J-E., Kwak M-H., Kim J-E., Lee Y-J., Kim R-U., Kim E-A., Lee G-Y., Kim D-S. and Hwang D-Y. Lab Anim Res, Vol.29(2), p103-112, Jun 2013.

24.

Water-soluble chitosan and herbal honey compound alleviates atopic dermatitis-like lesions in NC/Nga mice. C.Choi, W.-S.Kim, Y.H.Park, S.-C.Park, M.-K.Jang, J.-W.Nah. Journal of Industrial and Engineering Chemistry, May 2013.


25.

Interleukin-17/T-helper 17 cells in an atopic dermatitis mouse model aggravate orthodontic root resorption in dental pulp. M.Shimizu, M.Yamaguchi, S.Fujita, T.Utsunomiya, H.Yamamoto, K.Kasai. European Journal of Oral Sciences, Vol.121(2), p101-110, Apr 2013.


26.

Water-soluble chitosan and herbal honey compound alleviates atopic dermatitis-like lesions in NC/Nga mice. Choi C., Kim W-S., Park YH., Park S-C., Jang M-K., Nah J-W. Journal of Industrial and Engineering Chemistry, Vol.20(2), p499-504, Mar 2014.


27.

Indirubin, a purple 3,2- bisindole, inhibited allergic contact dermatitis via regulating T helper (Th)-mediated immune system in DNCB-induced model. Kim M H, Choi Y Y, Yang G, Cho I-H, Nam D, Yang W M. Journal of Ethnopharmacology, Vol.145(1), p214-219, Jan 2013.


28.

Effects of Catalpa ovata stem bark on atopic dermatitis-like skin lesions in NC/Nga mice. Yang G, Choi C-H, Lee K, Lee M, Ham I, Choi H-Y. Journal of Ethnopharmacology, Vol.145(2), p416-423, Jan 2013.


29.

Interleukin 33 Is Induced by Tumor Necrosis Factor α and Interferon γ in Keratinocytes and Contributes to Allergic Contact Dermatitis. Taniguchi K., Yamamoto S., Hitomi E., Inada Y., Suyama Y., Sugioka T., Hamasaki Y. J Investig Allergol Clin Immunol, Vol.23(6), p428-434, 2013.


30.

Organic Chemicals in Diesel Exhaust Particles Enhance Picryl Chloride-Induced Atopic Dermatitis in NC/Nga Mice. Sadakane K., Ichinose T., Takano H., Yanagisawa R., Inoue K., Kawazato H., Yasuda A., Hayakawa K. Int Arch Allergy Immunol, Vol.162(1), p7-15, 2013.


31.

The effects of (±)-Praeruptorin A on airway inflammation, remodeling and transforming growth factor-β1/Smad signaling pathway in a murine model of allergic asthma. Xiong Y, Wang J, Wu F, Li J, Kong L. International Immunopharmacology, Vol.14(4), p392-400, Dec 2012.


32.

Inhibitory effects of Drynaria fortunei extract on house dust mite antigen-induced atopic dermatitis in NC/Nga mice. Sung Y-Y, Kim D-S, Yang W-K, Nho K J, Seo H S, Kim Y S, Kim H K.. Journal of Ethnopharmacology, Vol.144(1), p94-100, Oct 2012.


33.

Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice. Sung Y-Y, Yang W-K, Lee A Y, Kim D-S, Nho K J, Kim Y S, Kim H K.. Journal of Ethnopharmacology, Vol.144(1), p151-159, Oct 2012.


34.

Anti-allergic effect of lactic acid bacteria isolated from seed mash used for brewing sake is not dependent on the total IgE levels. Masuda Y, Takahashi T, Yoshida K, Nishitani Y, Mizuno M, Mizoguchi H. Journal of Bioscience and Bioengineering, Vol.114(3), p292-296, Sep 2012.


35.

Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis. Matsushita A, Seike M, Okawa H, Kadawaki Y, Ohtsu H. Experimental Dermatology, Vol.21(9), p714-715, Sep 2012.


36.

Platycodon grandiflorus alleviates DNCB-induced atopy-like dermatitis in NC/Nga mice. Park S-J, Lee H-A, Kim J W, Lee B-S and Kim E-J. Indian J Pharmacol. Vol.44(4), p469-474, Jul-Aug 2012.


37.

Effect of Chrysanthemi borealis flos on atopic dermatitis induced by 1-chloro 2,4-dinitrobenzene in NC/Nga mouse. Yang G, Lee K, An D-G, Lee M-H, Ham I-H, Choi H-Y. Immunopharmacology and Immunotoxicology, Vol.34(3), p413-418, Jun 2012.


38.

Effects of (±)-praeruptorin A on airway inflammation, airway hyperresponsiveness and NF-κB signaling pathway in a mouse model of allergic airway disease. Xiong Y, Wang J, Wu F, Li J, Zhou L, Kong L. European Journal of Pharmacology, Vol.683(1-3), p316-324, May 2012.


39.

Anti-allergic effect of lactic acid bacteria isolated from seed mash used for brewing sake is not dependent on the total IgE levels. Y. Masuda., T. Takahashi., K. Yoshida., Y. Nishitani., M. Mizuno., H. Mizoguchi. Journal of Bioscience and Bioengineering, 2012.


40.

The Protective and Recovery Effects of Fish Oil Supplementation on Cedar Pollen-Induced Allergic Reactions in Mice. Hirao A, Furutani N, Nagahama H, Itokawa M, Shibata S. Food and Nutrition Sciences, Vol.3, p40-47, 2012.


41.

Th17-cells in atopic dermatitis stimulate orthodontic root resorption. Yamada K, Yamaguchi M, Asano M, Fujita S, Kobayashi R, Kasai K. Oral Diseases, 2012.


42.

Mixture of Polyphenols and Anthocyanins from Vaccinium uliginosum L. Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice. Kim M J and Choung S-Y. Evidence-Based Complementary and Alternative Medicine, Vol.2012 (2012)


43.

Single systemic administration of Ag85B of mycobacteria DNA inhibits allergic airway inflammation in a mouse model of asthma. Karamatsu K, Matsuo K, Inada H, Tsujimura Y, Shiogama Y, Matsubara A, Kawano M and Yasutomi Y. J Asthma Allergy, Vol.5, p71-79, 2012.


44.

Inhibitory Effect of Nelumbo nucifera (Gaertn.) on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. R. Karki., M.-A Jung., K.-J. Kim., and D.-W Kim. Evidence-Based Complementary and Alternative Medicine, Vol. 2012 (2012), Article ID 153568, 7 pages


45.

Lack of Transient Receptor Potential Vanilloid-1 Enhances Th2-Biased Immune Response of the Airways in Mice Receiving Intranasal, but Not Intraperitoneal, Sensitization. T. Mori., K. Saito., Y. Ohki., H. Arakawa., M. Tominaga., K. Tokuyama. Int Arch Allergy Immunol 2011, Vol.156, p305-312.


46.

Improvement of the Antiinflammatory and Antiallergic Activity of Bidens pilosa L. var. radiata SCHERFF Treated with Enzyme (Cellulosine). Horiuchi, M. and Seyama, Y. Journal of Health Science, Vol. 54(2008), No.3, p294-301.


47.

Suppression of spontaneous dermatitis in NC/Nga murine model by PG102 isolated from Actinidia arguta. Park, E.J., Park, K.C., Eo, H, Seo, J., Son, M., Kim, K.H. et al. J Investigative Dermatology, Vol.127, p1154-1160, 2007.


48.

Sopungyangjae-Tang inhibits development of dermatitis in Nc/Nga mice. Pokharel, Y.R., Lim, S.C., Kim, S.C., Heo, T.H., Choi, H.K. and Kang, K.W. Evidence-based Complementary and Alternative Medicine nem015, 1-8, March 12, 2007.


49.

Inhibitory effect of DA-9201, an extract of Oryza sativa L., on airway inflammation and bronchial hyperresponsiveness in mouse asthma model. Lee, S.H., Sohm, Y.S., Kang, K.K., Kwon, J.W. and Yoo, M. Biol. Pharm. Bull. Vol.29(6), p1148-1153, 2006.


50.

A new symbiotic, Lactobacillus casei subsp. casei together with dextran, reduces murine and human allergic reaction. Ogawa, T., Hashikawa, S., Asai, Y., Sakamoto, H., Yasuda, K., and Makimura, Y. FEMS Immunology and Medical Microbiology, Vol.46, p400-409, 2006.


51.

Effects of alpha tocopherol and probucol supplements on allergen-induced airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. Okamoto, N., Murata, T., Tamai, H., Tanaka, H., and Nagai, H. Int. Allergy Immunol. Vol.141, p172-180, 2006.


52.

Control of IgE and selective TH1 and TH2 cytokines by PG102 isolated from Actinidia arguta. Parkm E.J., Kim, B., Eo, H., Park, K, Kim, Y., Lee, H.J. and Son, M. J Allergy and Clinical Immunol, p1151-1167, 2005.


53.

Oral immunotherapy against a pollen allergy using a seed-based peptide vaccine. Takagi, H., Saito, S., Yang, L., Nagasawa, S., Nishizawa, N., and Takaiwa, F. Plant Biotechnology Journal, Vol.3, p521-533, 2005.


54.

Activated protein C inhibits bronchial hyperresponsiveness and Th2 cytokine expression in mice. Hisamichi Yuda, Yukihiko Adachi, Osamu Taguchi, Esteban C. Gabazza, Osamu Hataji, Hajime Fujimoto, Shigenori Tamaki, Kimiaki Nishikubo, Kenji Fukudome, Corina N. D’Alessandro-Gabazza, Junko Maruyama, Masahiko Izumizaki, Michiko Iwase, Ikuo Homma, Ryo Inoue, Haruhiko Kamada, Tatsuya Hayashi, Michael Kasper, Bart N. Lambrecht, Peter J. Barnes, and Koji Suzuki. Blood, Vol.103, No.6, p2196-2204, 2004.


55.

Inadequate induction of suppressor of cytokine signaling-1 causes systemic autoimmune diseases. Fujimoto, M., Tsutsui, H., Xinshou, O., Tokumoto, M., Watanabe, D., Shima, Y., Yoshimoto, T., Hirakata, H., Kawase, I., Nakanishi, K., Kishimoto, T., and Naka, T. International Immunology Vol.16, p303-314, 2004.


56.

Association of cathepsin E deficiency with development of atopic dermatitis. Tsukuba, T., Okamoto, K., Okamoto, Y., Yanagawa, M. J Biochemistry, Vol.134, p893-902, 2003.


57.

Mast-cell-dependent histamine release after praziquantel treatment of Schistosoma japonicum infection: implications for chemotherapy-related adverse effects. Jun Matsumoto and Hajime Matsuda. Parasitology Research, Vol.88, p888 – 893, 2002.


◆LBIS® 大鼠免疫球蛋白E(IgE) ELISA试剂盒相关参考文献


1.

Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT). Ryosuke Kaneko, Atsuko Sato, Shun Hamada, Takeshi Yagi, Ichiro Ohsawa, Mamitaro Ohtsuki, Eiji Kobayashi, Masumi Hirabayashi, Takashi Murakami. Transgenic Research, pp 1-12, First online: 17 February 2016


2.

Oral administration of drugs with hypersensitivity potential induces germinal center hyperplasia in secondary lymphoid organ/tissue in Brown Norway rats, and this histological lesion is a promising candidate as a predictive biomarker for drug hypersensitivity occurrence in humans. A.Tamura, I.Miyawaki, T.Yamada, J.Kimura, H.Funabashi. Toxicology and Applied Pharmacology, Vol.271(1), p30-40, Aug 2013.


3.

Enhancement of the pulmonary allergic granulocyte recruitment in rats exposed to DMTI-Ⅱ,a Kunitz-type inhibitor isolated from Dimorphandra mollis seeds. Glaucia,C,M.,Karina,F,R.,Dalize,M,S.,Andre,A,S.,Ivani,A,de S.,Maria,L R,M.,Edson,A.. International Immunopharmacology, Vol.11(6),  p740-747, Jun 2011.


4.

Altered cytokine expression in mesenteric lymph nodes in a rat strain (Matsumoto Eosinophilic Shinshyu) that spontaneously develops hypereosinophilia. Muto, S., Monnai, M., Okuhara, Y., Murakami, M., Kuroda, J., Ono, T., and Matsumoto, K.. Immunology, Vol.116, p373-380, 2005.


◆LBIS® 小鼠免疫球蛋白G(IgG)类风湿因子ELISA试剂盒相关参考文献


 1.

Dysbiosis Contributes to Arthritis Development via Activation of Autoreactive T Cells in the Intestine. Maeda Y, Kurakawa T, Umemoto E, Motooka D, Ito Y, Gotoh K, Hirota K, Matsushita M, Furuta Y, Narazaki M, Sakaguchi N, Kayama H, Nakamura S, Iida T, Saeki Y, Kumanogoh A, Sakaguchi S, Takeda K. Arthritis Rheumatol. 2016 Nov;68(11):2646-2661.


 2.

C9orf72 ablation causes immune dysregulation characterized by leukocyte expansion, autoantibody production, and glomerulonephropathy in mice. Atanasio A, Decman V, White D, Ramos M, Ikiz B, Lee HC, Siao CJ, Brydges S, LaRosa E, Bai Y, Fury W, Burfeind P, Zamfirova R, Warshaw G, Orengo J, Oyejide A, Fralish M, Auerbach W, Poueymirou W, Freudenberg J, Gong G, Zambrowicz B, Valenzuela D, Yancopoulos G, Murphy A, Thurston G, Lai KM. Sci Rep. 2016 Mar 16


 3.

Reciprocal effects of Schistosoma mansoni infection on spontaneous autoimmune arthritis in IL-1 receptor antagonist-deficient mice. Osada Y, Yamada S, Nakae S, Sudo K, Kanazawa T. Parasitol Int. Vol.64(1), p13-17. Feb 2015.


 4.

The development of mixed cryoglobulinemia in Capillaria hepatica-infected mice is associated with the capillaria antigen-induced selective proliferation of splenic B-1a cells in response to interleukin-5 stimulation. Aihara N, Kamiie J, Yamada M, Shirota K. Am J Pathol. Vol.185(1), p172-84, Jan 2015.


 5.

Mechanism of the 24-hour rhythm of tumor necrosis factor-alpha formed by onset of rheumatoid arthritis. Yoshimatsu H., Okazaki F., Ieiri I., To H. Chronobiology International, 2014.


 6.

Deficient Leptin Signaling Ameliorates Systemic Lupus Erythematosus Lesions in MRL/Mp-Faslpr Mice. Fujita Y., Fujii T., Mimori T., Sato T., Nakamura T., Iwao H., Nakajima A., Miki M., Sakai T., Kawanami T., Tanaka M., Masaki Y., Fukushima T., Okazaki T. and Umehara H. The Journal of Immunology, Vol.192(3), p979-984, Feb 2014.


 7.

Phenotype conversion from rheumatoid arthritis to systemic lupus erythematosus by introduction of Yaa mutation into FcγRIIB-deficient C57BL/6 mice. Kawano S, Lin Q, Amano H, Kaneko T, Nishikawa K, Tsurui H, Tada N, Nishimura H, Takai T, Shirai T, Takasaki Y, Hirose S. European Journal of Immunology, Vol.43(3), p770-778, Mar 2013.


 8.

IFN-γ-Producing Effector CD8 T Lymphocytes Cause Immune Glomerular Injury by Recognizing Antigen Presented as Immune Complex on Target Tissue. Tsumiyama K., Hashiramoto A., Takimoto M., Tsuji-Kawahara S., Miyazawa M. and Shiozawa S. The Journal of ImmunologyJuly, Vol.191(1), p91-96, 2013.


 9.

Increased expression of soluble form of vascular cell adhesion molecule-1 aggravates autoimmune arthritis in MRL-Fasipr mice. Oishi, H., Mizuki, S., Terada, M., Kudo, M., Araki, K., Araki, M., Nose, M., and Takahashi, S. Pathology International, Vol.57, p734-740, 2007.


10.

Electro-acupuncture at acupoint ST36 reduces inflammation and regulates immune activity in collagen-induced arthritic mice. Yim, Y.Y., Lee, H., Hong, K.E., Kim, Y.I., Lee, B.R., Son, C.G., and Kim, J.E. Evidence-based Complementary and Alternative Medicine 4:p51-57, 2007.

11.

Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Kumanogoh, A., Shikina, T., Watanabe, C., Takegahara, N., Suzuki, K., Yamamoto, M., Takamatsu, H., Prasad, D.V.R., Mizui, M., Toyohuku, T., Tamaru, M., Watanabe, D., Parnes, J.R., and Kikutani, H. International Immuno, Vol.17, p1277-1282, 2005.


12.

The point mutation of tyrosine 759 of the IL-6 family cytokine receptor gp130 synergizes with HTLV-1 pX in promoting rheumatoid arthritis-like arthritis. Ishihara, K., Sawa, S., Ikushima, H., Hirota, S., Atsumi, T., Kamimura, D., Park, S.J., Murakami, M., Kitanuma, Y., Iwakura, Y., and Hirano, T. International Immunol. Vol.16, p455-465, 2004.


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Chemical induction of HO-1 suppresses lupus nephritis by reducing local iNOS expression and synthesis of anti-dsDNA antibody. Takeda, Y., Takeno, M., Iwasaki, M., Kobayashi, H., Kirino, Y., Ueda, A., Nagahama, K., Aoki, I., and Ishigatsubo, Y. Clin Exp Immunol. Vol.138, p237-244, 2004.


14.

A Point Mutation of Tyr-759 in Interleukin 6 Family Cytokine Receptor Subunit gp130 Causes Autoimmune Arthritis. T. Atsumi., K. Ishihara., D. Kamimura., H. Ikushima., T. Ohtani., S. Hirota., H. Kobayashi., S. Park., Y. Saeki., Y. Kitamura. and T. Hirano. J Exp Med, 196, Number 7, p979-990, 2002.


15.

A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice. Kikukawa, T, Kojima, M., and Abe, C. Jap J Inflammation, Vol.20, p697-701, 2000.


◆LBIS® 小鼠免疫球蛋白M(IgM)类风湿因子ELISA试剂盒相关参考文献


 1.

C9orf72 ablation causes immune dysregulation characterized by leukocyte expansion, autoantibody production, and glomerulonephropathy in mice.
Atanasio A, Decman V, White D, Ramos M, Ikiz B, Lee HC, Siao CJ, Brydges S, LaRosa E, Bai Y, Fury W, Burfeind P, Zamfirova R, Warshaw G, Orengo J, Oyejide A, Fralish M, Auerbach W, Poueymirou W, Freudenberg J, Gong G, Zambrowicz B, Valenzuela D, Yancopoulos G, Murphy A, Thurston G, Lai KM.

Sci Rep. 2016 Mar 16

 2.

Production of Autoantibodies by Murine B-1a Cells Stimulated with Helicobacter pylori Urease through Toll-Like Receptor 2 Signaling
Kobayashi F, Watanabe E, Nakagawa Y, Yamanishi S, Norose Y, Fukunaga Y and Takahashi H.

Infect. Immun, Vol.79(12), p4791-4801, Dec 2011.


 3.

Lack of B and T lymphocyte attenuator exacerbates autoimmune disorders and induces Fas-independent liver injury in MRL-lpr/lpr mice
Y. Oya., N. Watanabe. Y. Kobayashi., T. Owada., M. Oki., K. Ikeda. A. Suto., S. Kagami., K. Hirose., T. Kishimoto., and H. Nakajima.

Int. Immunol. Vol.23(5), p335-344, 2011.


 4.

An experimental type 2 mixed cryoglobulinemia with renal glomerulopathy in ICR mice triggered by Capillaria hepatica infection
N. Aihara., J. K. Chambers., K. Hosono., J. Kamiie., and K. Shirota.

Histol Histopathol, Vol.26, p1519-1529, 2011.


 5.

Point mutation of tyrosine 759 of the IL-6 family cytokine receptor,gp130,augments collagen-induced arthritis in DBA/IJ mice
Tsuji,F.,Yoshimi,M.,Katsuta,O.,Takai,M.,Ishihara,K.,Aono,H.

BMC Musculoskeletal Disorders ,10:23, 2009.


 6.

Implications for induction of autoimmunity via activation of B-1 cells by Helicobacter pylori urease.
Yamasnishi, S., Iizumi, T., Watanabe, E., Shimizu, M., Kamiya, S., Nagata, K., Kumagai, Y., Fukunaga, Y., and Takahashi, H.

Infection and Immunity, Vol.74, p248-256, 2006.


 7.

Cytokine production profile of splenocytes derived from zymosan A-treated SKG mice developing arthritis.
Kobayashi, K., Suda, T., Nan-ya, K., Sakaguchi, N., Sakaguchi, S., and Miki, I.

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 8.

Chronic polyarthritis caused by mammalian DNA that escapes from degradation in macrophages.
Kawane, K., Ohtani, M., Miwa, K., Kizawa, T., Kanbara, Y., Yoshioka, Y., and Yoshikawa,H.

Nature, Vol.443, p998-1002, 2006.


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Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
Kumanogoh, A., Shikina, T., Watanabe, C., Takegahara, N., Suzuki, K., Yamamoto, M., Takamatsu, H., Prasad, D.V.R., Mizui, M., Toyohuku, T., Tamaru, M., Watanabe, D., Parnes, J.R., and Kikutani, H.

International Immuno, Vol.17, p1277-1282, 2005.


10.

A Point Mutation of Tyr-759 in Interleukin 6 Family Cytokine Receptor Subunit gp130 Causes Autoimmune Arthritis
T. Atsumi., K. Ishihara., D. Kamimura., H. Ikushima., T. Ohtani., S. Hirota., H. Kobayashi., S. Park., Y. Saeki., Y. Kitamura. and T. Hirano

J Exp Med, 196, Number 7, p979-990, 2002.


11.

A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice.
Kikukawa, T, Kojima, M., and Abe, C.

Jap J Inflammation, Vol.20, p697-701, 2000.



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LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒 LBIS® OVA-IgE Mouse

产品中心 > 生命科学 > 疾病研究 > LBIS® 动物代谢检测试剂盒系列

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒
LBIS® OVA-IgE Mouse

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒                              LBIS® OVA-IgE Mouse小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒

 


  IgE(Immunoglobulin E,免疫球蛋白E)是第五个被发现的免疫球蛋白,由5个结构域(VH、CHε 1~4)构成的两个Hε链和两个L链中组成的IgE,其分子量约190000,电泳实验中向γ1域移动。IgE的代谢半衰期约3天,正常人血清中的IgE浓度非常低,约300 ng/mL。但在寄生虫感染和枯草热时浓度会升高。与过敏性相关的IgE被称作反应素。因接触过敏原导致反应素含量升高,反应素会在Fc域与存在于皮肤、呼吸道、消化脏器中嗜碱性粒细胞和肥大细胞的FcεR1受体结合,引起细胞过敏。在结合过敏原后细胞发生脱粒现象,组胺,五羟色胺,蛋白酶,肝素,趋化因子,前列腺素,白三烯等被投放,经过支气管收缩和黏膜水肿,分泌亢进,从而诱发支气管哮喘,部分荨麻疹,过敏性鼻炎,过敏性反应等I型过敏性反应。

  本试剂盒是以OVA(卵清蛋白)作为免疫的抗原,在一个简化的反应系统中通过测定鼠抗OVA- IgE抗体值来进行小鼠免疫系统检测的试剂盒。

◆特点

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒                              LBIS® OVA-IgE Mouse


● 测定时间短(总反应时间:1小时50分钟)。

● 微量样本即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

● 操作简便,无需进行特殊前处理。

 

试剂盒组成


组成品

状态

包装

OVA包被96孔板(干燥板)

清洗后使用

96 wells(8×12)/1个

标准溶液(Anti OVA-IgE:1,200 U/mL)(单抗)

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgE抗体(单抗)

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4)※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


◆样本信息


● 小鼠血清·血浆

● 10 μL/well(稀释样本)

※ 样本需要用试剂盒附带的缓冲液调至标准曲线范围内。

※ 样本必须稀释10倍以上。

 


测定范围


1.88~120 U/mL(标准曲线范围)

(本试剂盒中1 U/mL定义为抗原结合常数(Ka)为2.0×108 M-1的抗体1.3 ng/mL)

 


实验数据


精度测试(组内变异)


样本

A

B

1

70.7

19.1

2

71.0

18.7

3

77.1

19.6

4

74.3

19.7

5

72.9

18.9

Mean

73.2

19.2

SD

2.6

0.42

CV(%)

3.6

2.2

单位:U/mL



重复性测试(组间变异)


测定日/样本

C

D

E

0天

60.0

15.0

3.75

1天

59.1

15.0

3.75

2天

58.1

14.7

3.65

3天

63.6

16.0

3.48

Mean

60.2

15.2

3.66

SD

2.4

0.57

0.13

CV(%)

4.0

3.7

3.4

单位:U/mL



加标回收测试


样本F


添加量

实测值

回收量

回收率(%)

0.00

6.93

5.35

12.2

5.27

98.5

10.7

17.8

10.9

102

17.1

25.1

18.2

106

单位:U/mL,n=3


样本G


添加量

实测值

回收量

回收率(%)

0.00

40.5

30.8

70.4

29.5

95.8

35.9

77.1

36.6

102

53.9

94.9

54.4

101

单位:U/mL,n=3



稀释直线性测试


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.9987~0.9999

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639-07651 (AKRIE-030)小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒
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96 tests

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