6X His Peptide (6X His多肽)

产品编号

JJP91-1mg
6X His Peptide (6X His多肽)
5mg/ml×0.2ml

182

JJP91-5mg
6X His Peptide (6X His多肽)
5mg/ml×1ml

617

JJP91-25mg
6X His Peptide (6X His多肽)
25mg

2068

6X His Peptide (6X His多肽),也称6X His-tag Peptide (6X His标签多肽)、6X His tag Peptide (6X His tag多肽)或His-tag Peptide (His标签多肽),是一种常用于通过竞争性结合Anti-His抗体(也称His-tag antibody、His标签抗体、6X His antibody或6X His抗体),从而在免疫沉淀时洗脱与Anti-His抗体结合的6X His融合表达蛋白的多肽。本产品不仅可以用于Anti-His Magnetic Beads (Anti-His磁珠) (JP2021)免疫沉淀时6X His融合表达蛋白的洗脱,也可以用于常规的Anti-His抗体免疫沉淀时6X His融合表达蛋白的洗脱。
本产品竞争性洗脱6X His融合表达蛋白后,洗脱后的样品中不含Anti-His抗体的轻链和重链,可以有效解决免疫沉淀后Western检测时轻链和重链的干扰问题。普通的免疫沉淀,通常是通过加入上样缓冲液煮沸从而使His融合表达蛋白进入到溶液中,但此时Anti-His抗体的轻链和重链也会进入到溶液中,并很可能会干扰后续的Western检测。
6X His Peptide (6X His多肽)是合成的多肽,其氨基酸序列为His-His-His-His-His-His (HHHHHH)。
6X His Peptide (6X His多肽)的主要参数如下:

About this protein

Product name
6X His Peptide (6X His多肽)

Alternative names
His Tag; His Synthetic Peptide; His Tag Peptide; Hexa-histidine; Hexa His Tag Peptide

Description
6X His Peptide is a synthetic peptide with a molecular weight of 840.85. The amino acid sequence is His-His-His-His-His-His.

Purity
~98% by HPLC

Molecular Formula
C36H44N18O7

Molecular Weight
~840.85

Sequence (Three-Letter Code)
His-His-His-His-His-His

Sequence (One-Letter Code)
HHHHHH

Source
Synthetic

Reconstitution
TBS (50mM Tris-HCl, pH7.4, 150mM NaCl)

本产品5mg/ml包装产品配制在TBS中,可以直接使用。25mg为粉末装。6X His Peptide (6X His多肽)常用储存液浓度为5-25mg/ml,常用使用浓度为100-400μg/ml。
包装清单:

产品编号
产品名称
包装

JJP91-1mg
6X His Peptide (6X His多肽)
5mg/ml×0.2ml

JJP91-5mg
6X His Peptide (6X His多肽)
5mg/ml×1ml

JJP91-25mg
6X His Peptide (6X His多肽)
25mg


说明书
1份

保存条件:
-20℃保存,至少一年有效。JJP91-25mg粉末装也可以4℃保存,一年有效。
注意事项:
由于多肽的每次冻融均会引起部分失活,所以溶液装或粉末装配制成溶液后须分装后于-20℃或更低温度冻存,以避免反复冻融。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

DO Supplement -His/-Leu/-Trp/-Ura 与Minimal SD base联合使用

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/色氨酸(Trp)/尿嘧啶(Ura)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成四组分缺陷的酵母菌选择性培养基,俗称四缺培养基。用于酵母双杂交系统和酵母三杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                 名称                                                    规格           价格(元)  
DO Supplement -Ade/-His/-Leu/-Trp 10g /
DO Supplement -His/-Leu/-Met/-Trp 10g /
DO Supplement -His/-Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

 培养基类型                                           名称                                                 货号                   终浓度(g/L)  
 Rich Media

 富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
 Minimal Media

 基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
 Single Dropout Supplements (SDO)

 单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
 Double Dropout Supplements (DDO)

 双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Leu/-Met/-Trp 酵母菌的质粒筛选 营养缺陷型突变菌株

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/蛋氨酸(Met)/色氨酸(Trp)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成四组分缺陷的酵母菌选择性培养基,俗称四缺培养基。用于酵母双杂交系统和酵母三杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                 名称                                                    规格           价格(元)  
DO Supplement -Ade/-His/-Leu/-Trp 10g /
DO Supplement -His/-Leu/-Met/-Trp 10g /
DO Supplement -His/-Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

DO Supplement -His/-Leu/-Met/-Trp 本品为缺乏组氨酸(His)/亮氨酸(Leu)/蛋氨酸(Met)/色氨酸(Trp)的省却成分培养基添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/蛋氨酸(Met)/色氨酸(Trp)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成四组分缺陷的酵母菌选择性培养基,俗称四缺培养基。用于酵母双杂交系统和酵母三杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                 名称                                                    规格           价格(元)  
DO Supplement -Ade/-His/-Leu/-Trp 10g /
DO Supplement -His/-Leu/-Met/-Trp 10g /
DO Supplement -His/-Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

DO Supplement -His/-Leu/-Trp

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/色氨酸(Trp)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成三组分缺陷的酵母菌选择性培养基,俗称三缺培养基。用于双质粒酵母转化筛选和报告基因检测。可用于外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                  名称                                             规格            价格(元)  
DO Supplement -His/-Leu/-Trp 10g  /
DO Supplement -His/-Leu/-Ura 10g  /
DO Supplement -His/-Trp/-Ura 10g  /
DO Supplement -Leu/-Met/-Trp 10g  /
DO Supplement -Leu/-Trp/-Ura 10g  /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Leu/-Ura

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/尿嘧啶(Ura)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成三组分缺陷的酵母菌选择性培养基,俗称三缺培养基。用于双质粒酵母转化筛选和报告基因检测。可用于外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                   名称                                             规格            价格(元)  
DO Supplement -His/-Leu/-Trp 10g /
DO Supplement -His/-Leu/-Ura 10g /
DO Supplement -His/-Trp/-Ura 10g /
DO Supplement -Leu/-Met/-Trp 10g /
DO Supplement -Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

SD/-His/-Leu/-Met/-Trp Broth 酵母缺陷型培养基(肉汤)

YPD Medium(YEPD Medium);YPDA Medium;YNB; Minimal SD Base;Dropout (DO) Supplements氨基酸缺失混合物;

产品信息

产品名称    规格             
SD/-Ade/-His/-Leu/-Trp Broth 酵母缺陷型培养基(肉汤)         

产品描述

酵母缺陷型培养基是由SD Base + DO Supplement,以添加琼脂和未添加琼脂两种方式混合而成的完全培养基,前者是固体培养基,后者是液体培养基(肉汤),用于选择培养含特异载体的酵母或进行杂交筛选。

Minimal SD Base,即Minimal Synthetical Defined Medium,是在无氨基酸酵母氮源基础(YNB)上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement,即氨基酸缺陷混合物(或省却成分添加剂)是缺乏一种或多种特定氨基酸的混合物。

SD-Ade-His-Leu-Trp Broth就是缺乏腺嘌呤(Ade),组氨酸(His),亮氨酸(Leu)和色氨酸(Trp)四种成分的酵母缺陷型液体培养基,主要用于双质粒酵母转化筛选和双/三杂交系统报告基因检测。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

培养基配制

1)   称取28g粉末加入1L去离子水,搅拌混匀使其溶解。

2)  重要:测试培养基pH是否为5.8,若有必要用弱酸或弱碱调整pH。

3)   121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

附表1 酵母培养基配制浓度表

培养基类型 名称 货号 终浓度(g/L)
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
DO Supplement -Trp/-His/-Ade MS6336-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp    MS6341-10G        1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15
SD + DO Broth酵母缺陷型完全培养基(肉汤) SD/-His Broth MS6361-5L 28
SD/-Leu Broth MS6363-5L 28
SD/-Trp Broth MS6365-5L 28
SD/-Ura Broth MS6367-5L 28
SD/-His/-Leu Broth MS6381-5L 28
SD/-His/-Ura Broth MS6383-5L 28
SD/-Leu/-Trp Broth MS6385-5L 28
SD/-Met/-Trp Broth MS6387-5L 28
SD/-Trp/-Ura Broth MS6389-5L 28
SD/-Trp/-His Broth MS6391-5L 28
SD/-Trp/-Ade Broth MS6393-5L 28
SD/-Ura/-Leu Broth MS6395-5L 28

上接附表1酵母培养基配制浓度表

培养基类型 名称 货号 终浓度(g/L)
SD + DO Broth酵母缺陷型完全培养基(肉汤) SD/-His/-Leu/-Trp Broth MS6411-5L         28
SD/-His/-Leu/-Ura Broth MS6413-5L 28
SD/-His/-Trp/-Ura Broth MS6415-5L 28
SD/-Leu/-Met/-Trp Broth MS6417-5L 28
SD/-Leu/-Trp/-Ura Broth MS6419-5L 28
SD/-Trp/-His/-Ade Broth MS6421-5L 28
SD/-Ade/-His/-Leu/-Trp Broth MS6431-5L 28
SD/-His/-Leu/-Met/-Trp Broth MS6433-5L 28
SD/-His/-Leu/-Trp/-Ura Broth MS6435-5L 28
SD + DO with Agar酵母缺陷型完全培养基(琼脂)  SD/-His with Agar MS6362-5L ——
SD/-Leu with Agar MS6364-5L ——
SD/-Trp with Agar MS6366-5L ——
SD/-Ura with Agar MS6368-5L ——
SD/-His/-Leu with Agar MS6382-5L ——
SD/-His/-Ura with Agar MS6384-5L ——
SD/-Leu/-Trp with Agar MS6386-5L ——
SD/-Met/-Trp with Agar MS6388-5L ——
SD/-Trp/-Ura with Agar MS6390-5L ——
SD/-Trp/-His with Agar MS6392-5L       ——
SD/-Trp/-Ade with Agar MS6394-5L ——
SD/-Ura/-Leu with Agar MS6396-5L ——
SD/-His/-Leu/-Trpwith Agar          MS6412-5L ——
SD/-His/-Leu/-Urawith Agar MS6414-5L ——
SD/-His/-Trp/-Urawith Agar MS6416-5L ——
SD/-Leu/-Met/-Trpwith Agar MS6418-5L ——
SD/-Leu/-Trp/-Urawith Agar MS6420-5L ——
SD/-Trp/-His/-Adewith Agar MS6422-5L ——
SD/-Ade/-His/-Leu/-Trpwith Agar MS6432-5L ——
SD/-His/-Leu/-Met/-Trpwith Agar MS6434-5L ——
SD/-His/-Leu/-Trp/-Urawith Agar MS6436-5L ——

 

DO Supplement -His/-Leu,酵母培养基配制浓度表

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

 产品名称                             

            规格            

    

     

 DO Supplement -His/-Leu    10g    
 DO Supplement -His/-Leu    100g    

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                 名称                                         规格               价格(元)  
DO Supplement -His/-Leu 10g /
DO Supplement -His/-Ura 10g /
DO Supplement -Leu/-Trp 10g /
DO Supplement -Met/-Trp 10g /
DO Supplement -Trp/-Ura 10g /
DO Supplement -Trp/-His 10g /
DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Leu

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

 产品名称                             

 产品编号             规格            

     

     

 DO Supplement -His/-Leu  MS6320-10G  10g  418  318
 DO Supplement -His/-Leu  MS6320-100G  100g  2518  1998

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                 名称                                         规格               价格(元)  
DO Supplement -His/-Leu 10g /
DO Supplement -His/-Ura 10g /
DO Supplement -Leu/-Trp 10g /
DO Supplement -Met/-Trp 10g /
DO Supplement -Trp/-Ura 10g /
DO Supplement -Trp/-His 10g /
DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Ade


描述

DO Supplement -His/-Ade 

产品标签

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

 

  产品名称                                产品编号            规格             价格(元)      促销价(元)   
  DO Supplement -His/-Ade    MS6327-10G   10g   418   318
  DO Supplement -His/-Ade    MS6327-100G   100g   2518   1998

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/腺嘌呤(Ade)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                         规格               价格(元)  
MS6320-10G DO Supplement -His/-Leu 10g /
MS6321-10G DO Supplement -His/-Ura 10g /
MS6322-10G DO Supplement -Leu/-Trp 10g /
MS6323-10G DO Supplement -Met/-Trp 10g /
MS6324-10G DO Supplement -Trp/-Ura 10g /
MS6325-10G DO Supplement -Trp/-His 10g /
MS6326-10G DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

 

DO Supplement -His/-Ura Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

  产品名称                                             规格               
  DO Supplement -His/-Ura      10g
  DO Supplement -His/-Ura      100g

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/尿嘧啶(Ura)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                         规格               价格(元)  
MS6320-10G DO Supplement -His/-Leu 10g /
MS6321-10G DO Supplement -His/-Ura 10g /
MS6322-10G DO Supplement -Leu/-Trp 10g /
MS6323-10G DO Supplement -Met/-Trp 10g /
MS6324-10G DO Supplement -Trp/-Ura 10g /
MS6325-10G DO Supplement -Trp/-His 10g /
MS6326-10G DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

本品为缺乏组氨酸(His)的省却成分培养基添加剂 DO Supplement -His

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成单组分缺陷的酵母菌选择性培养基,俗称单缺培养基(一缺培养基),主要用于单质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

                  名称                                                                    规格              价格(元)    
DO Supplement -His 省却成分添加剂-组氨酸 10g /
DO Supplement -Leu 省却成分添加剂-亮氨酸 10g /
DO Supplement -Trp 省却成分添加剂-色氨酸 10g /
DO Supplement -Ura 省却成分添加剂-尿嘧啶 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                               货号                  终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

DO Supplement -His/-Ade Dropout (DO) Supplements 省却成分添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

  产品名称                                产品编号            规格                      
  DO Supplement -His/-Ade    MS6327-10G   10g     
  DO Supplement -His/-Ade    MS6327-100G   100g      

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/腺嘌呤(Ade)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                         规格               价格(元)  
MS6320-10G DO Supplement -His/-Leu 10g /
MS6321-10G DO Supplement -His/-Ura 10g /
MS6322-10G DO Supplement -Leu/-Trp 10g /
MS6323-10G DO Supplement -Met/-Trp 10g /
MS6324-10G DO Supplement -Trp/-Ura 10g /
MS6325-10G DO Supplement -Trp/-His 10g /
MS6326-10G DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Leu/-Trp/-Ura Dropout (DO) Supplements 省却成分添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

  产品名称                                                  产品编号            规格          
  DO Supplement -His/-Leu/-Trp/-Ura   MS6343-10G   10g
  DO Supplement -His/-Leu/-Trp/-Ura   MS6343-100G   100g

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/色氨酸(Trp)/尿嘧啶(Ura)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成四组分缺陷的酵母菌选择性培养基,俗称四缺培养基。用于酵母双杂交系统和酵母三杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                                    规格           价格(元)  
MS6341-10G DO Supplement -Ade/-His/-Leu/-Trp 10g /
MS6342-10G DO Supplement -His/-Leu/-Met/-Trp 10g /
MS6343-10G DO Supplement -His/-Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

 培养基类型                                           名称                                                 货号                   终浓度(g/L)  
 Rich Media

 富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
 Minimal Media

 基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
 Single Dropout Supplements (SDO)

 单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
 Double Dropout Supplements (DDO)

 双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Leu/-Met/-Trp Dropout (DO) Supplements 省却成分添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

  产品名称                                                  产品编号             规格         
  DO Supplement -His/-Leu/-Met/-Trp   MS6342-10G   10g
  DO Supplement -His/-Leu/-Met/-Trp   MS6342-100G   100g

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/蛋氨酸(Met)/色氨酸(Trp)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成四组分缺陷的酵母菌选择性培养基,俗称四缺培养基。用于酵母双杂交系统和酵母三杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                                    规格           价格(元)  
MS6341-10G DO Supplement -Ade/-His/-Leu/-Trp 10g /
MS6342-10G DO Supplement -His/-Leu/-Met/-Trp 10g /
MS6343-10G DO Supplement -His/-Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

DO Supplement -His/-Leu/-Ura Dropout (DO) Supplements 省却成分添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

  产品名称                                      产品编号            规格           
  DO Supplement -His/-Leu/-Ura   MS6332-10G   10g
  DO Supplement -His/-Leu/-Ura   MS6332-100G   100g

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/尿嘧啶(Ura)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成三组分缺陷的酵母菌选择性培养基,俗称三缺培养基。用于双质粒酵母转化筛选和报告基因检测。可用于外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                   名称                                             规格            价格(元)  
MS6331-10G DO Supplement -His/-Leu/-Trp 10g /
MS6332-10G DO Supplement -His/-Leu/-Ura 10g /
MS6333-10G DO Supplement -His/-Trp/-Ura 10g /
MS6334-10G DO Supplement -Leu/-Met/-Trp 10g /
MS6335-10G DO Supplement -Leu/-Trp/-Ura 10g /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

DO Supplement -His/-Leu/-Trp Dropout (DO) Supplements 省却成分添加剂

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

  产品名称                                       产品编号           规格            
  DO Supplement -His/-Leu/-Trp MS6331-10G 10g
  DO Supplement -His/-Leu/-Trp MS6331-100G 100g

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/亮氨酸(Leu)/色氨酸(Trp)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成三组分缺陷的酵母菌选择性培养基,俗称三缺培养基。用于双质粒酵母转化筛选和报告基因检测。可用于外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析(His3,Ade2)。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                   名称                                             规格            价格(元)  
MS6331-10G DO Supplement -His/-Leu/-Trp 10g  /
MS6332-10G DO Supplement -His/-Leu/-Ura 10g  /
MS6333-10G DO Supplement -His/-Trp/-Ura 10g  /
MS6334-10G DO Supplement -Leu/-Met/-Trp 10g  /
MS6335-10G DO Supplement -Leu/-Trp/-Ura 10g  /

表1 酵母培养基配制浓度表

培养基类型                                           名称                                                 货号                   终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

SD/-His/-Leu/-Trp/-Ura Broth 酵母缺陷型培养基(肉汤)


描述

SD/-His/-Leu/-Trp/-Ura Broth

产品标签

YPD Medium(YEPD Medium);YPDA Medium;YNB; Minimal SD Base;Dropout (DO) Supplements氨基酸缺失混合物;

产品信息

产品名称 产品编号   规格           价格(元)  
SD/-His/-Leu/-Trp/-Ura Broth 酵母缺陷型培养基(肉汤)   MS6435-5L       5L 958

产品描述

酵母缺陷型培养基是由SD Base + DO Supplement,以添加琼脂和未添加琼脂两种方式混合而成的完全培养基,前者是固体培养基,后者是液体培养基(肉汤),用于选择培养含特异载体的酵母或进行杂交筛选。

Minimal SD Base,即Minimal Synthetical Defined Medium,是在无氨基酸酵母氮源基础(YNB)上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement,即氨基酸缺陷混合物(或省却成分添加剂)是缺乏一种或多种特定氨基酸的混合物。

SD/-His/-Leu/-Trp/-Ura Broth就是缺乏组氨酸(His),亮氨酸(Leu),色氨酸(Trp)和尿嘧啶(Ura)四种成分的酵母缺陷型液体培养基,主要用于双质粒酵母转化筛选和双/三杂交系统报告基因检测。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

培养基配制

1)   称取28g粉末加入1L去离子水,搅拌混匀使其溶解。

2)  重要:测试培养基pH是否为5.8,若有必要用弱酸或弱碱调整pH。

3)   121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

附表1 酵母培养基配制浓度表

培养基类型 名称 货号 终浓度(g/L)
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
DO Supplement -Trp/-His/-Ade MS6336-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp    MS6341-10G        1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15
SD + DO Broth酵母缺陷型完全培养基(肉汤) SD/-His Broth MS6361-5L 28
SD/-Leu Broth MS6363-5L 28
SD/-Trp Broth MS6365-5L 28
SD/-Ura Broth MS6367-5L 28
SD/-His/-Leu Broth MS6381-5L 28
SD/-His/-Ura Broth MS6383-5L 28
SD/-Leu/-Trp Broth MS6385-5L 28
SD/-Met/-Trp Broth MS6387-5L 28
SD/-Trp/-Ura Broth MS6389-5L 28
SD/-Trp/-His Broth MS6391-5L 28
SD/-Trp/-Ade Broth MS6393-5L 28
SD/-Ura/-Leu Broth MS6395-5L 28

上接附表1酵母培养基配制浓度表

培养基类型 名称 货号 终浓度(g/L)
SD + DO Broth酵母缺陷型完全培养基(肉汤) SD/-His/-Leu/-Trp Broth MS6411-5L         28
SD/-His/-Leu/-Ura Broth MS6413-5L 28
SD/-His/-Trp/-Ura Broth MS6415-5L 28
SD/-Leu/-Met/-Trp Broth MS6417-5L 28
SD/-Leu/-Trp/-Ura Broth MS6419-5L 28
SD/-Trp/-His/-Ade Broth MS6421-5L 28
SD/-Ade/-His/-Leu/-Trp Broth MS6431-5L 28
SD/-His/-Leu/-Met/-Trp Broth MS6433-5L 28
SD/-His/-Leu/-Trp/-Ura Broth MS6435-5L 28
SD + DO with Agar酵母缺陷型完全培养基(琼脂)  SD/-His with Agar MS6362-5L ——
SD/-Leu with Agar MS6364-5L ——
SD/-Trp with Agar MS6366-5L ——
SD/-Ura with Agar MS6368-5L ——
SD/-His/-Leu with Agar MS6382-5L ——
SD/-His/-Ura with Agar MS6384-5L ——
SD/-Leu/-Trp with Agar MS6386-5L ——
SD/-Met/-Trp with Agar MS6388-5L ——
SD/-Trp/-Ura with Agar MS6390-5L ——
SD/-Trp/-His with Agar MS6392-5L       ——
SD/-Trp/-Ade with Agar MS6394-5L ——
SD/-Ura/-Leu with Agar MS6396-5L ——
SD/-His/-Leu/-Trpwith Agar          MS6412-5L ——
SD/-His/-Leu/-Urawith Agar MS6414-5L ——
SD/-His/-Trp/-Urawith Agar MS6416-5L ——
SD/-Leu/-Met/-Trpwith Agar MS6418-5L ——
SD/-Leu/-Trp/-Urawith Agar MS6420-5L ——
SD/-Trp/-His/-Adewith Agar MS6422-5L ——
SD/-Ade/-His/-Leu/-Trpwith Agar MS6432-5L ——
SD/-His/-Leu/-Met/-Trpwith Agar MS6434-5L ——
SD/-His/-Leu/-Trp/-Urawith Agar MS6436-5L ——

 

 

 

DO Supplement -His/-Ura


描述

DO Supplement -His/-Ura

 

产品标签

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

 

  产品名称                               产品编号              规格           价格(元)   促销价(元)  
  DO Supplement -His/-Ura   MS6321-10G   10g 418 318
  DO Supplement -His/-Ura   MS6321-100G   100g 2518 1998

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)/尿嘧啶(Ura)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                         规格               价格(元)  
MS6320-10G DO Supplement -His/-Leu 10g /
MS6321-10G DO Supplement -His/-Ura 10g /
MS6322-10G DO Supplement -Leu/-Trp 10g /
MS6323-10G DO Supplement -Met/-Trp 10g /
MS6324-10G DO Supplement -Trp/-Ura 10g /
MS6325-10G DO Supplement -Trp/-His 10g /
MS6326-10G DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15

 

 

DO Supplement -His


描述

DO Supplement –His

产品标签

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

  产品名称                                                        产品编号          规格            价格(元)    促销价(元) 
  DO Supplement -His 省却成分添加剂-组氨酸    MS6316-10G   10g   418   318
  DO Supplement -His 省却成分添加剂-组氨酸    MS6316-100G   100g   2518   1998

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏组氨酸(His)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成单组分缺陷的酵母菌选择性培养基,俗称单缺培养基(一缺培养基),主要用于单质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                    名称                                                                    规格              价格(元)    
MS6316-10G DO Supplement -His 省却成分添加剂-组氨酸 10g /
MS6317-10G DO Supplement -Leu 省却成分添加剂-亮氨酸 10g /
MS6318-10G DO Supplement -Trp 省却成分添加剂-色氨酸 10g /
MS6319-10G DO Supplement -Ura 省却成分添加剂-尿嘧啶 10g /

 

表1 酵母培养基配制浓度表

培养基类型                                            名称                                               货号                  终浓度(g/L)  
Rich Media

富集培养基

YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15