Optimized for transient gene expression, and production of viral vectors and recombinant proteins.
Details
BalanCD HEK293 medium is a versatile, chemically-defined, animal component-free medium optimized for transfection and production of viral vectors and recombinant proteins.
One medium supports multiple applications including:
Rapid, scalable production of viral vectors for gene therapy (adeno-associated viruses (AAV), lentiviruses)
Transient protein expression and recombinant protein production to gram-scale yields
Formulated for versatility and ease-of-use:
Use the same growth medium for seeding, transfection and production
Scalable protocol for use in flasks, spinner flasks and bioreactors
Add complementary BalanCD HEK293 Feed and/or Anti-Clumping Supplement to maximize growth and titer
Fulfills regulatory requirements:
Chemically-defined, animal-component free
Drug Master File (DMF) filed with US FDA
Also available as Powder: .
Simple, scalable protocol supports cell growth and increases yield of lentiviral vector
Increase protein yield compared to other commercially-available media
Optimized for transient gene expression, and production of viral vectors and recombinant proteins.
Details
BalanCD HEK293 medium is a versatile, chemically-defined, animal component-free medium optimized for transfection and production of viral vectors and recombinant proteins.
Standard Packaging Size: 10 L
Larger pack sizes available upon request
Also Available as Liquid:
One medium supports multiple applications including:
Rapid, scalable production of viral vectors for gene therapy (adeno-associated viruses (AAV), lentiviruses)
Transient protein expression and recombinant protein production to gram-scale yields
Formulated for versatility and ease-of-use:
Use the same growth medium for seeding, transfection and production
Scalable protocol for use in flasks, spinner flasks and bioreactors
Add complementary BalanCD HEK293 Feed and/or Anti-Clumping Supplement to maximize growth and titer
Fulfills regulatory requirements:
Chemically-defined, animal-component free
Drug Master File (DMF) filed with US FDA
Simple, scalable protocol supports cell growth and increases yield of lentiviral vector
Increase protein yield compared to other commercially-available media
Enhance post-transfection growth and protein production of multiple HEk293 cell lines
Details
BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.
Increase viable cell density and culture duration to maximize yield
Use the same Feed for a wide range of HEK293 cell lines
Fulfill regulatory requirements
Chemically-defined, animal component-free formula
Drug Master File (DMF) submitted to US FDA
Two-fold increase in titer compared to other commercially-available media
HEK293-6E cells were cultured in duplicate 125mL Erlenmeyer flasks with a 30 mL working volume. Cells were seeded at 3 x 105 cells/mL in BalanCD HEK 293 medium. PEI-mediated transfection was performed on day 3 to produce a biosimilar antibody. BalanCD HEK293 medium was supplemented with a 5% v/v addition of BalanCD HEK293 Feed on days 1-4 post-transfection. Protein titer was measured on days 7, 9, and 11 post-transfection. Cultures terminated when viability dropped below 70%, (data not shown). Data shown represents the average of two duplicate cultures.
Boost viral vector production in HEK293 cells up to 10-fold.
Details
The First Viral Feed Designed Specifically for HEK293 Cells
Gene therapy and viral vector-based vaccine manufacturers rely on basal media as the standard to grow their adeno-associated (AAV) viral vectors. The first-of-its-kind BalanCD HEK293 Viral Feed from FUJIFILM Irvine Scientific delivers a 3-fold to 10-fold increase in viral vector production over basal media alone to provide consistent performance, scalability, and speed time-to-market.
Scalable and adaptable viral vector performance
BalanCD HEK293 Viral Feed can be used with HEK293 suspension cultures from small- to large-scale for AAV production of multiple serotypes, as well as a range of agnostic basal growth media and transfection methods.
Chemically defined BalanCD HEK293 Viral Feed, optimized to enhance viral vector production
Supplement to HEK293 suspension cultures post-transfection and supports high cell densities
A complete solution to achieve viral vector production goals for gene therapy applications
Optimized for use with chemically defined medium and compatible with other basal growth media
Multiple-fold increases in viral titers
BalanCD HEK293 Viral Feed promotes vigorous VG/capsid yields and multiple-fold increases in titers to help manufacturers get more working volume for their applications.
AAV2 titer improvement upon feed
Figure 1. AAV2 viral genome titer and capsid titer were measured using cell extracts subjected to three cycles of freeze-thaw, followed by clarification by centrifugation and treatment with DNAse I and lysis buffer prior to qPCR (using AAVpro titration kit, Takara Bio) and ELISA (AAV2 titration ELISA, Progen). Blue and orange bars indicate the viral genome (VG) and capsid titer fold over the basal culture without a feed. The data indicates that the feed media enhancing the AAV2 production approximately 10-fold in VG and 6-fold for the capsid titer. As an overall result, viral packaging was determined to improve about 67%, from 13% in Basal to 21.81% in Basal+feed (data not shown).
BalanCD HEK293 Viral Feed increases the AAV titer in 10 liter scale-up
Figure 2. Verification of the BalanCD HEK293 Viral Feed performance on improving the production of AAV5 in 10L bioreactor scale. HEK293 suspension cells (cell line 2) were seeded at density of 0.8X10^6 cell/mL in 8.3 L culture volume in 10L bioreactor (XDR10 bioreactor). BalanCD HEK293 Viral Feed was added at 12% v/v at 24 hr post transfection. Titer was measured using ddPCR.
An uninterrupted supply of feed for HEK293 cell lines
BalanCD HEK293 Viral Feed is manufactured in a GMP facility using qualified raw materials sourced from a solid supply chain to ensure continuity of supply and lot-to-lot reliability for HEK293-specific applications. Our stringent oversight provides confirmation of formula, analysis, and assurance that BalanCD HEK293 Viral Feed meets the highest global and regional standards while fulfilling regulatory demands with each manufacturing lot file.
Stringent raw materials control and sourcing program
cGMP-compliant manufacturing
COA, COO, TSE/BSE statements
ISO13485, EN 13485:2016 certified
Drug Master Files (DMF) supported
for other packaging sizes and powder format availability.
