描述

Ethidium Monoazide Bromide (EMA) 叠氮溴化乙锭

产品关键词：

Ethidium Monoazide Bromide (EMA) 叠氮溴化乙锭；Ethidium bromide (EB) 溴化乙锭；Photoafinity label 光亲和标记核酸染料；EMA-PCR；死细菌和活细胞区分；CAS NO：58880-05-0；

产品信息

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产品描述

叠氮溴化乙锭（Ethidium Monoazide Bromide，EMA）是一种具光亲和标记特性的荧光核酸染料。EMA经光分解后，能共价结合到溶液和损伤的细胞核酸上。此染料能用来印记DNA上的药物结合位点，检测死细胞，鉴定DNA和tRNA上的乙锭结合位点和选择性失活基因表达。EMA最特殊的用途是标记死活细胞群内的死细胞，因其相对来说不具有细胞膜渗透性，则选择性结合死细胞的DNA。EMA还能通过5′核酸酶PCR来区分死细菌和活细菌。EMA本身荧光较弱，当与DNA结合后荧光增强约15倍，最大激发/发射波长约510/600nm。

产品特性

1）   CAS NO：58880-05-0

2）   同义名：3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide; Ethidium bromide monoazide; 溴化乙锭单叠氮；溴化乙锭叠氮化物；叠氮溴乙锭；

3）   分子式：C21H18BrN5

4）   分子量：420 g/mol

5）   外观：橙色固体

6）   纯度：≥95% (HPLC)

7）   EX/Em：510/600nm（与核酸结合）

8）   溶解性：溶于DMF、无水乙醇

9）   化学结构式：

保存与运输方法

保存：-20℃避光干燥保存，至少1年有效。

运输：室温运输。

应用示例

文献来源1）Rudi K et al. Use of Ethidium Monoazide and PCR in Combination for Quantification of Viable and Dead Cells in Complex Samples. Appl Environ Microbiol. 2005 Feb;71(2):1018-24.

Fig 1. Schematic representation of EMA-PCR. (A) EMA is added to the test sample containing both viable and dead cells. EMA penetrates the dead cells and binds to the DNA. Light exposure for 1 min leads to covalent binding and inactivation of free EMA. EMA does not enter viable cells. (B) There are two populations of DNA after purification. The DNA population from viable cells is unstained, while the DNA from the dead cells is covalently bound to EMA. (C). The unstained DNA from viable cells is PCR amplified, while the DNA from dead cells with bound EMA cannot be amplified.

EMA-PCR：EMA was added to samples at a final concentration of 100 μg/ml. The samples were then incubated in the dark for 5 min and subsequently exposed to light for 1 min. The light source was an Osram SLG 1000 with a 650-W halogen lightbulb, which was placed 20 cm from the sample tubes. The microcentrifuge tubes were placed on ice prior to light exposure to minimize elevated temperature in the samples.

注意事项

1）   荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）   为了您的安全和健康，请穿实验服并戴一次性手套操作。

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