CellEase® II DNA提取试剂
DNA extraction reagent : CellEase® II series
◆原理
此款试剂盒用于细胞内 DNA 提取，操作极为简便，提取的 DNA 可直接用于下游PCR 操作，无需纯化步骤，仅需 9 分钟。CellEase® II 跟其他产品相比无需洗脱，更可提高回收效率。方法简单，只需将样品与 CellEase A和B混合，并进行2步温控孵育即可。
该试剂盒可从动物组织、微生物、植物细胞等样品中制备 DNA。该试剂盒不仅用于常规实验室，也可用在食品检测方面。
◆优点・特色
● 快速高效提取DNA
● 简单操作即可制备PCR的DNA模板
● 成本低
● 用于实验室和食品检测
DNA 提取试剂 CellEase® II 系列产品
◆案例・应用
【实验步骤】
● 实验前，请先准备 CellEase® 混合液，按 1：1：3 的比例混合溶液 A、B 和 ddH2O。
● 将样品切成合适的大小，放入离心管中。
● 向管中加入 CellEase® 混合液。
● 72℃下孵育样品 6 分钟。
● 接着在 94℃条件下孵育样品 3分钟。
● 即可得到纯化 DNA，取适量作为模板，进行 PCR实验。
【实验案例】
<小鼠尾巴DNA 提取与检测>
M ：Marker (100bp ladder)
1 ：CellEaseII, Extracted solution was undiluted
2 ：CellEaseII, Extracted solution was ×10 diluted
3 ：CellEaseII, Extracted solution was ×100 diluted
4 ：Conventional CellEase , Extracted solution was undiluted
5 ：Conventional CellEase , Extracted solution was ×10 diluted
6 ：Conventional CellEase , Extracted solution was ×100 diluted
<全血DNA 提取与检测>
Sample: Whole blood (EDTA)
M: Marker (100 bp ladder)
1: CellEase® Blood
2: CellEase® Blood
3: CellEase® Blood
4: Extraction by water
<金黄色葡萄球菌DNA提取与检测>
|
M Marker (500 bp ladder)
1: Undiluted Sample Add 5 μl of DNA extract to PCR
2: x 10 dilution
3: x 102 dilution
4: x 103 dilution
5: x 104 dilution
|
6: Undiluted Sample Add 6 μl of DNA extract to PCR
7: x 10 dilution
8: x 102 dilution
9: x 103 dilution
10: x 104 dilution
|
<番茄叶DNA提取与检测>
|
M: Marker（500 bp ladder）
1: + CellEase®, Without dilution Add 5 μl to PCR
2: + CellEase®, ×10 dilution Add 5 μl to PCR
3: + CellEase®, × 100 dilution Add 5 μl to PCR
4: – CellEase®, Without dilution Add 5 μl to PCR
5: – CellEase®, ×10 dilution Add 5 μl to PCR
6: – CellEase®, × 100 dilution Add 5 μl to PCR
7: + CellEase®, Without dilution Add 6 μl to PCR
8: + CellEase®, ×10 dilution Add 6 μl to PCR
9: + CellEase®, × 100dilution Add 6 μl to PCR
|
10: – CellEase®，Without dilution Add 6 μl to PCR
11: – CellEase®，×10 dilution Add 6 μl to PCR
12: – CellEase®，×100 dilution Add 6 μl to PCR
13: + CellEase®，Without dilution Add 7 μl to PCR
14: + CellEase®，×10 dilution Add 7 μl to PCR
15: + CellEase®，×100 dilution Add 7 μl to PCR
16: – CellEase®，Without dilution Add 7 μl to PCR
17: – CellEase®，×10 dilution Add 7 μl to PCR
18: – CellEase®，×100 dilution Add 7 μl to PCR
|
<金枪鱼DNA提取与检测>
M: Marker（100 bp ladder）
1: Sample 6 mg, Add 5 μl of DNA extract to PCR
2: Sample 6 mg, Add 6 μl of DNA extract to PCR
3: Sample 6 mg, Add 7 μl of DNA extract to PCR
4: Sample 9 mg, Add 5 μl of DNA extract to PCR
5: Sample 9 mg, Add 6 μl of DNA extract to PCR
6: Sample 9 mg, Add 7 μl of DNA extract to PCR
7: Sample 18 mg, Add 5 μl of DNA extract to PCR
8: Sample 18 mg Add 6 μl of DNA extract to PCR
9: Sample 18 mg Add 7 μl of DNA extract to PCR
DNA分离PS-快速试剂
