溶剂体积

浓度

体内研究（动物）：

动物模型（Animal Model）：Hairless pigmented (black) guinea pig models

药物配制（Preparation）：dA (DeoxyArbutin) was prepared in I base cream consisting of water (25%), 0.2M triethanolamine hydrochloride (44.5%), glycerin (3.75%), Parson MCX (7.5%), cutina GMS (2.5%), and tegin 90 (2.5%).

实验方法（Assay）：For experiments comparing skin lightening effects of test compounds, skin was treated daily for 9 weeks with dA, Hydroquinone (HQ), kojic acid and arbutin (3%) in I base cream. Ten animals were in each treatment group, and three independent experiments were performed. Skin lightness was measured at baseline and then weekly with a colorimeter.

实验结果（Results）：Both dA and HQ resulted in skin lightening during the initial 3 weeks of treatment, the extent of dA-induced skin lightening plateaued while the HQ-treated skin darkened and returned to the pretreatment value. The dA treated sites showed no signs of skin irritation. In contrast, HQ treated skin showed signs of irritation that resulted in irregular darkening, beginning at 3 weeks of treatment. In contrast, kojic acid, and arbutin were not significantly different from the vehicle control.

体内研究（细胞）：

细胞类型（Cell type）：Human melanocytes

药物配制（Preparation）：All compounds (dA, dF, fdA, tdA and HQ) were dissolved in sterile DMSO. Stock solutions of compounds were prepared and protected from light at -20℃until use. At time of use, compounds were further diluted in growth medium to produce the final required concentration. Compounds were tested at the appropriate concentration range (1-100μM) in triplicate in order to calculate LD50values.

实验方法（Assay）：Melanocytes were seeded at 1-3×105cells and allowed to grow for 48h before treatment. Cultured were treated daily with fresh growth media containing test compound for 5 days. On the sixth day, cells were detached and counted.

实验结果（Results）：A concentration-dependent decrease in the cell number of both was observed for all inhibitors (dA, dF, fdA, tdA and HQ). LD50concentrations of dA and its derivatives are listed below.

溶剂体积

浓度

体内研究（动物）：

动物模型（Animal Model）：Hairless pigmented (black) guinea pig models

药物配制（Preparation）：dA (DeoxyArbutin) was prepared in I base cream consisting of water (25%), 0.2M triethanolamine hydrochloride (44.5%), glycerin (3.75%), Parson MCX (7.5%), cutina GMS (2.5%), and tegin 90 (2.5%).

实验方法（Assay）：For experiments comparing skin lightening effects of test compounds, skin was treated daily for 9 weeks with dA, Hydroquinone (HQ), kojic acid and arbutin (3%) in I base cream. Ten animals were in each treatment group, and three independent experiments were performed. Skin lightness was measured at baseline and then weekly with a colorimeter.

实验结果（Results）：Both dA and HQ resulted in skin lightening during the initial 3 weeks of treatment, the extent of dA-induced skin lightening plateaued while the HQ-treated skin darkened and returned to the pretreatment value. The dA treated sites showed no signs of skin irritation. In contrast, HQ treated skin showed signs of irritation that resulted in irregular darkening, beginning at 3 weeks of treatment. In contrast, kojic acid, and arbutin were not significantly different from the vehicle control.

体内研究（细胞）：

细胞类型（Cell type）：Human melanocytes

药物配制（Preparation）：All compounds (dA, dF, fdA, tdA and HQ) were dissolved in sterile DMSO. Stock solutions of compounds were prepared and protected from light at -20℃until use. At time of use, compounds were further diluted in growth medium to produce the final required concentration. Compounds were tested at the appropriate concentration range (1-100μM) in triplicate in order to calculate LD50values.

实验方法（Assay）：Melanocytes were seeded at 1-3×105cells and allowed to grow for 48h before treatment. Cultured were treated daily with fresh growth media containing test compound for 5 days. On the sixth day, cells were detached and counted.

实验结果（Results）：A concentration-dependent decrease in the cell number of both was observed for all inhibitors (dA, dF, fdA, tdA and HQ). LD50concentrations of dA and its derivatives are listed below.