文献1，Chiadak JD et al. Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB. Mediators Inflamm. 2016;2016:1431789. Epub 2016 Nov 2. PMID: 27881903

诱导方法：To induce adipocyte differentiation,3T3-L1 murine preadipocyte cells (day 2) were incubated for 60 h in DMEM supplemented with 10% fetal bovine serum and containing 500 µM IBMX, 0.25 µM dexamethasone, and 10 µg/mL insulin. The cells were then maintained in the culture medium supplemented with insulin only and this media was changed every 2 days (day 5 and day 7) until complete differentiation (monitored by lipid droplet accumulation under the microscope and confirmed by Oil Red Coloration) had occurred (day 9).

文献2，Mudhasani R et al. An Essential Role for Dicer in Adipocyte Differentiation.J Cell Biochem. 2010 Jul 1;110(4):812-6. PMID: 20564208

诱导方法：Mouse embryonic fibroblasts (MEFs)or preadipocytes were then plated at 50–60% confluence and stimulated two days later (considered as day 0 [D0]) with a hormonal cocktail composed of 0.25mM 3-Isobutyl-1-methylxanthine (IBMX), 0.1µM Dexamethasone, Insulin at 10µg/ml and 1µM Rosiglitazone. Starting on day two (D2), the media was replaced every two days with fresh DMEM supplemented with Insulin (10µg /ml) and 1µM Rosiglitazone. Cells were harvested 7–9 days post-induction, fixed in 10% formalin for Oil Red O (ORO) staining.

文献3，Rafieemehr H et al. Improving the neuronal differentiation efficiency of umbilical cord blood-derived mesenchymal stem cells cultivated under appropriate conditions. Iran J Basic Med Sci. 2015 Nov;18(11):1100-6. PMID: 26949497

诱导方法：To induce differentiation into adipocytes, cells were plated at 1,000 cells/cm2in 24-well plates in DMEM with 1 μM dexamethasone, 10 μg/ml insulin, 0.5 mM IBMX, and 100 μM indomethacin. After 2 wk of adipogenic stimulation, cells were fixed in 5% PFA for 30 min and incubated with Oil Red-O to stain lipid vacuoles.

文献1，Chiadak JD et al. Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB. Mediators Inflamm. 2016;2016:1431789. Epub 2016 Nov 2. PMID: 27881903

诱导方法：To induce adipocyte differentiation,3T3-L1 murine preadipocyte cells (day 2) were incubated for 60 h in DMEM supplemented with 10% fetal bovine serum and containing 500 µM IBMX, 0.25 µM dexamethasone, and 10 µg/mL insulin. The cells were then maintained in the culture medium supplemented with insulin only and this media was changed every 2 days (day 5 and day 7) until complete differentiation (monitored by lipid droplet accumulation under the microscope and confirmed by Oil Red Coloration) had occurred (day 9).

文献2，Mudhasani R et al. An Essential Role for Dicer in Adipocyte Differentiation.J Cell Biochem. 2010 Jul 1;110(4):812-6. PMID: 20564208

诱导方法：Mouse embryonic fibroblasts (MEFs)or preadipocytes were then plated at 50–60% confluence and stimulated two days later (considered as day 0 [D0]) with a hormonal cocktail composed of 0.25mM 3-Isobutyl-1-methylxanthine (IBMX), 0.1µM Dexamethasone, Insulin at 10µg/ml and 1µM Rosiglitazone. Starting on day two (D2), the media was replaced every two days with fresh DMEM supplemented with Insulin (10µg /ml) and 1µM Rosiglitazone. Cells were harvested 7–9 days post-induction, fixed in 10% formalin for Oil Red O (ORO) staining.

文献3，Rafieemehr H et al. Improving the neuronal differentiation efficiency of umbilical cord blood-derived mesenchymal stem cells cultivated under appropriate conditions. Iran J Basic Med Sci. 2015 Nov;18(11):1100-6. PMID: 26949497

诱导方法：To induce differentiation into adipocytes, cells were plated at 1,000 cells/cm2in 24-well plates in DMEM with 1 μM dexamethasone, 10 μg/ml insulin, 0.5 mM IBMX, and 100 μM indomethacin. After 2 wk of adipogenic stimulation, cells were fixed in 5% PFA for 30 min and incubated with Oil Red-O to stain lipid vacuoles.

文献1，Chiadak JD et al. Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB. Mediators Inflamm. 2016;2016:1431789. Epub 2016 Nov 2. PMID: 27881903

诱导方法：To induce adipocyte differentiation,3T3-L1 murine preadipocyte cells (day 2) were incubated for 60 h in DMEM supplemented with 10% fetal bovine serum and containing 500 µM IBMX, 0.25 µM dexamethasone, and 10 µg/mL insulin. The cells were then maintained in the culture medium supplemented with insulin only and this media was changed every 2 days (day 5 and day 7) until complete differentiation (monitored by lipid droplet accumulation under the microscope and confirmed by Oil Red Coloration) had occurred (day 9).

文献2，Mudhasani R et al. An Essential Role for Dicer in Adipocyte Differentiation.J Cell Biochem. 2010 Jul 1;110(4):812-6. PMID: 20564208

诱导方法：Mouse embryonic fibroblasts (MEFs)or preadipocytes were then plated at 50–60% confluence and stimulated two days later (considered as day 0 [D0]) with a hormonal cocktail composed of 0.25mM 3-Isobutyl-1-methylxanthine (IBMX), 0.1µM Dexamethasone, Insulin at 10µg/ml and 1µM Rosiglitazone. Starting on day two (D2), the media was replaced every two days with fresh DMEM supplemented with Insulin (10µg /ml) and 1µM Rosiglitazone. Cells were harvested 7–9 days post-induction, fixed in 10% formalin for Oil Red O (ORO) staining.

文献3，Rafieemehr H et al. Improving the neuronal differentiation efficiency of umbilical cord blood-derived mesenchymal stem cells cultivated under appropriate conditions. Iran J Basic Med Sci. 2015 Nov;18(11):1100-6. PMID: 26949497

诱导方法：To induce differentiation into adipocytes, cells were plated at 1,000 cells/cm2in 24-well plates in DMEM with 1 μM dexamethasone, 10 μg/ml insulin, 0.5 mM IBMX, and 100 μM indomethacin. After 2 wk of adipogenic stimulation, cells were fixed in 5% PFA for 30 min and incubated with Oil Red-O to stain lipid vacuoles.