标签归档：脱氧

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖  CAS：154-17-6

发表于由

2-Deoxy-D-glucose (2-DG) 2-脱氧-D-葡萄糖；2-deoxyglucose；Hexokinase 己糖激酶；glycolysis糖酵解抑制剂； CAS：154-17-6

产品信息

产品名称

CAS NO.

规格

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

154-17-6

1g

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

154-17-6

5g

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

154-17-6

25g

产品描述

2-脱氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一种不可代谢的葡萄糖类似物，通过作用于己糖激酶（糖酵解的限速步骤）来抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖异构酶进一步代谢，进而引起2-DG-P在细胞内的累积和细胞内ATP的耗尽。体外缺氧模型中，2-DG导致细胞周期抑制和细胞死亡，诱导自噬，增加ROS生成和活化AMPK。体内动物模型中阻断肿瘤细胞生长。

产品特性

1） CAS NO：154-17-6

2） 化学名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol

3） 同义名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脱氧葡萄糖；2-去氧-D-葡萄糖；D-2-脱氧葡萄糖；

4） 分子式：C6H12O5

5） 分子量：164.16

6） 外观：白色至类白色固体

7） 纯度：>98%

8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）

9）化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，至少2年有效。 

运输：室温运输。

注意事项

1） 本品储存液置于≤-20℃避光保存，尽量避免反复冻融。通常情况下，-20℃至少一个月稳定，-80℃至少6个月稳定。

2） 本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

储存液配制

          质量

溶剂体积

浓度

 1mg 5mg 10mg
1mM 6.0916 mL 30.4581 mL 60.9162mL
5mM 1.2183mL 6.0916 mL 12.1832mL
10mM 0.6092 mL 3.0458 mL 6.0916 mL
50mM 0.1218 mL 0.6092 mL 1.2183 mL

使用方法【源自文献，仅作参考】

文献1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689

体外研究（细胞实验）：

细胞类型（Cell types）：MCF-7 cells

实验方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm. 

实验结果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).

体内研究（动物模型）：

动物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.

实验结果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
文献2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277

体内研究（动物模型）：

动物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age

配制方法（Formulation）：2-DGwasdiluted insaline. 

实验方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).

实验结果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.

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2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

发表于由

描述

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

产品标签

2-Deoxy-D-glucose (2-DG) 2-脱氧-D-葡萄糖；2-deoxyglucose；Hexokinase 己糖激酶；glycolysis糖酵解抑制剂； CAS：154-17-6

产品信息

产品名称

产品编号

CAS NO.

规格

价格（元）

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

MS0871-0001G

154-17-6

1g

200

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

MS0871-0005G

154-17-6

5g

500

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

MS0871-0025G

154-17-6

25g

1900

 

产品描述

2-脱氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一种不可代谢的葡萄糖类似物，通过作用于己糖激酶（糖酵解的限速步骤）来抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖异构酶进一步代谢，进而引起2-DG-P在细胞内的累积和细胞内ATP的耗尽。体外缺氧模型中，2-DG导致细胞周期抑制和细胞死亡，诱导自噬，增加ROS生成和活化AMPK。体内动物模型中阻断肿瘤细胞生长。

产品特性

1） CAS NO：154-17-6

2） 化学名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol

3） 同义名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脱氧葡萄糖；2-去氧-D-葡萄糖；D-2-脱氧葡萄糖；

4） 分子式：C6H12O5

5） 分子量：164.16

6） 外观：白色至类白色固体

7） 纯度：>98%

8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）

9）化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，至少2年有效。 

运输：室温运输。

注意事项

1） 本品储存液置于≤-20℃避光保存，尽量避免反复冻融。通常情况下，-20℃至少一个月稳定，-80℃至少6个月稳定。

2） 本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

储存液配制

          质量

溶剂体积

浓度

 1mg 5mg 10mg
1mM 6.0916 mL 30.4581 mL 60.9162mL
5mM 1.2183mL 6.0916 mL 12.1832mL
10mM 0.6092 mL 3.0458 mL 6.0916 mL
50mM 0.1218 mL 0.6092 mL 1.2183 mL

使用方法【源自文献，仅作参考】

文献1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689

体外研究（细胞实验）：

细胞类型（Cell types）：MCF-7 cells

实验方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm. 

实验结果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).

体内研究（动物模型）：

动物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.

实验结果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
文献2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277

体内研究（动物模型）：

动物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age

配制方法（Formulation）：2-DGwasdiluted insaline. 

实验方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).

实验结果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.

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2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

发表于由

描述

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

产品标签

2-Deoxy-D-glucose (2-DG) 2-脱氧-D-葡萄糖；2-deoxyglucose；Hexokinase 己糖激酶；glycolysis糖酵解抑制剂； CAS：154-17-6

产品信息

产品名称

产品编号

CAS NO.

规格

价格（元）

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

MS0871-0001G

154-17-6

1g

200

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

MS0871-0005G

154-17-6

5g

500

2-Deoxy-D-glucose 2-脱氧-D-葡萄糖

MS0871-0025G

154-17-6

25g

1900

 

产品描述

2-脱氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一种不可代谢的葡萄糖类似物，通过作用于己糖激酶（糖酵解的限速步骤）来抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖异构酶进一步代谢，进而引起2-DG-P在细胞内的累积和细胞内ATP的耗尽。体外缺氧模型中，2-DG导致细胞周期抑制和细胞死亡，诱导自噬，增加ROS生成和活化AMPK。体内动物模型中阻断肿瘤细胞生长。

产品特性

1） CAS NO：154-17-6

2） 化学名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol

3） 同义名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脱氧葡萄糖；2-去氧-D-葡萄糖；D-2-脱氧葡萄糖；

4） 分子式：C6H12O5

5） 分子量：164.16

6） 外观：白色至类白色固体

7） 纯度：>98%

8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）

9）化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，至少2年有效。 

运输：室温运输。

注意事项

1） 本品储存液置于≤-20℃避光保存，尽量避免反复冻融。通常情况下，-20℃至少一个月稳定，-80℃至少6个月稳定。

2） 本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

储存液配制

          质量

溶剂体积

浓度

 1mg 5mg 10mg
1mM 6.0916 mL 30.4581 mL 60.9162mL
5mM 1.2183mL 6.0916 mL 12.1832mL
10mM 0.6092 mL 3.0458 mL 6.0916 mL
50mM 0.1218 mL 0.6092 mL 1.2183 mL

使用方法【源自文献，仅作参考】

文献1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689

体外研究（细胞实验）：

细胞类型（Cell types）：MCF-7 cells

实验方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm. 

实验结果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).

体内研究（动物模型）：

动物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.

实验结果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
文献2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277

体内研究（动物模型）：

动物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age

配制方法（Formulation）：2-DGwasdiluted insaline. 

实验方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).

实验结果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.

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 MS0019-10G  Kanamycin Sulfate硫酸卡那霉素 10g
 MS0029-1G  Cefotaxime Sodium Salt噻孢霉素钠（头孢噻肟钠） 1g
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Deoxyarbutin 脱氧熊果苷

发表于由

描述

Deoxyarbutin 脱氧熊果苷

产品标签

Deoxyarbutin 脱氧熊果苷；β-Arbutin β-熊果苷；Tyrosinase inhibitor 酪氨酸酶抑制剂；Melanin production inhibitor 黑色素生成抑制剂；Polyphenol Oxidase 蘑菇多酚氧化酶；CAS：53936-56-4；

产品信息

产品名称 产品编号 规格              价格（元）    
Deoxyarbutin 脱氧熊果苷      MZ95033-1G         1g 360
Deoxyarbutin 脱氧熊果苷 MZ95033-5G 5g 780

产品描述

脱氧熊果苷（Deoxyarbutin，CAS：53936-56-4）是一种有效且可逆的酪氨酸酶抑制剂（IC50=50nM）。能抑制黑色素合成，引起明显和长期的皮肤增白效果。

产品特性

1）   CAS NO：53936-56-4

2）   化学名：4-(Oxan-2-yloxy)phenol

3）   分子式：C11H14O3

4）   分子量：194.23

5）   纯度：≥99%

6）   外观：白色或类白色粉末

7）   溶解性：溶于DMSO（100mM）、乙醇（100mM）、不溶于水

8）   化学结构图：

保存与运输方法

保存：-20ºC干燥保存，至少2年有效。

运输：室温运输

注意事项

1）   本品不是临床药物，只能用于科研用途，不能用于诊断或临床用途。

2）   为了您的安全和健康，请穿实验服并戴一次性手套操作。

配制储存液

         质量

溶剂体积

浓度

 1mg                      5mg                   10mg                  100mg              
1mM               5.1485 mL 25.7427 mL 51.4854 mL 514.8535 mL
5mM 1.0297 mL 5.1485 mL 10.2971 mL 102.9707 mL
10mM 0.5149 mL 2.5743 mL 5.1485 mL 51.4854 mL
50mM 0.1030 mL 0.5149 mL 1.0297 mL 10.2971 mL

使用方法【源自文献，仅作参考】

文献1，Boissy RE et al.DeoxyArbutin: A novel reversible tyrosinase inhibitor with effective in vivo skin lightening potency.Exp Dermatol. 2005 Aug;14(8):601-8. PMID: 16026582

体内研究（动物）：

动物模型（Animal Model）：Hairless pigmented (black) guinea pig models

药物配制（Preparation）：dA (DeoxyArbutin) was prepared in I base cream consisting of water (25%), 0.2M triethanolamine hydrochloride (44.5%), glycerin (3.75%), Parson MCX (7.5%), cutina GMS (2.5%), and tegin 90 (2.5%).

实验方法（Assay）：For experiments comparing skin lightening effects of test compounds, skin was treated daily for 9 weeks with dA, Hydroquinone (HQ), kojic acid and arbutin (3%) in I base cream. Ten animals were in each treatment group, and three independent experiments were performed. Skin lightness was measured at baseline and then weekly with a colorimeter.

实验结果（Results）：Both dA and HQ resulted in skin lightening during the initial 3 weeks of treatment, the extent of dA-induced skin lightening plateaued while the HQ-treated skin darkened and returned to the pretreatment value. The dA treated sites showed no signs of skin irritation. In contrast, HQ treated skin showed signs of irritation that resulted in irregular darkening, beginning at 3 weeks of treatment. In contrast, kojic acid, and arbutin were not significantly different from the vehicle control.
文献2，Chawla S et al. Mechanism of tyrosinase inhibition by deoxyArbutin and its second-generation derivatives.. Br J Dermatol. 2008 Dec;159(6):1267-74. PMID:18811684

体内研究（细胞）：

细胞类型（Cell type）：Human melanocytes

药物配制（Preparation）：All compounds (dA, dF, fdA, tdA and HQ) were dissolved in sterile DMSO. Stock solutions of compounds were prepared and protected from light at -20℃until use. At time of use, compounds were further diluted in growth medium to produce the final required concentration. Compounds were tested at the appropriate concentration range (1-100μM) in triplicate in order to calculate LD50values.

实验方法（Assay）：Melanocytes were seeded at 1-3×105cells and allowed to grow for 48h before treatment. Cultured were treated daily with fresh growth media containing test compound for 5 days. On the sixth day, cells were detached and counted.

实验结果（Results）：A concentration-dependent decrease in the cell number of both was observed for all inhibitors (dA, dF, fdA, tdA and HQ). LD50concentrations of dA and its derivatives are listed below.

LD50(dark melanocytes) LD50(light melanocytes)
dA (deoxyarbutin) 57.7±1.5 104.7±1.0
tdA (thideoxyarbutin) 48.0±2.0 87.8±2.5
fdA (fluorodeoxyarbutin) 89.1±0.7 106±1.5

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MZ95033-1G Deoxyarbutin 脱氧熊果苷 1g
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Deoxyarbutin 脱氧熊果苷

发表于由

描述

Deoxyarbutin 脱氧熊果苷

产品标签

Deoxyarbutin 脱氧熊果苷；β-Arbutin β-熊果苷；Tyrosinase inhibitor 酪氨酸酶抑制剂；Melanin production inhibitor 黑色素生成抑制剂；Polyphenol Oxidase 蘑菇多酚氧化酶；CAS：53936-56-4；

产品信息

产品名称 产品编号 规格              价格（元）    
Deoxyarbutin 脱氧熊果苷      MZ95033-1G         1g 360
Deoxyarbutin 脱氧熊果苷 MZ95033-5G 5g 780

产品描述

脱氧熊果苷（Deoxyarbutin，CAS：53936-56-4）是一种有效且可逆的酪氨酸酶抑制剂（IC50=50nM）。能抑制黑色素合成，引起明显和长期的皮肤增白效果。

产品特性

1）   CAS NO：53936-56-4

2）   化学名：4-(Oxan-2-yloxy)phenol

3）   分子式：C11H14O3

4）   分子量：194.23

5）   纯度：≥99%

6）   外观：白色或类白色粉末

7）   溶解性：溶于DMSO（100mM）、乙醇（100mM）、不溶于水

8）   化学结构图：

保存与运输方法

保存：-20ºC干燥保存，至少2年有效。

运输：室温运输

注意事项

1）   本品不是临床药物，只能用于科研用途，不能用于诊断或临床用途。

2）   为了您的安全和健康，请穿实验服并戴一次性手套操作。

配制储存液

         质量

溶剂体积

浓度

 1mg                      5mg                   10mg                  100mg              
1mM               5.1485 mL 25.7427 mL 51.4854 mL 514.8535 mL
5mM 1.0297 mL 5.1485 mL 10.2971 mL 102.9707 mL
10mM 0.5149 mL 2.5743 mL 5.1485 mL 51.4854 mL
50mM 0.1030 mL 0.5149 mL 1.0297 mL 10.2971 mL

使用方法【源自文献，仅作参考】

文献1，Boissy RE et al.DeoxyArbutin: A novel reversible tyrosinase inhibitor with effective in vivo skin lightening potency.Exp Dermatol. 2005 Aug;14(8):601-8. PMID: 16026582

体内研究（动物）：

动物模型（Animal Model）：Hairless pigmented (black) guinea pig models

药物配制（Preparation）：dA (DeoxyArbutin) was prepared in I base cream consisting of water (25%), 0.2M triethanolamine hydrochloride (44.5%), glycerin (3.75%), Parson MCX (7.5%), cutina GMS (2.5%), and tegin 90 (2.5%).

实验方法（Assay）：For experiments comparing skin lightening effects of test compounds, skin was treated daily for 9 weeks with dA, Hydroquinone (HQ), kojic acid and arbutin (3%) in I base cream. Ten animals were in each treatment group, and three independent experiments were performed. Skin lightness was measured at baseline and then weekly with a colorimeter.

实验结果（Results）：Both dA and HQ resulted in skin lightening during the initial 3 weeks of treatment, the extent of dA-induced skin lightening plateaued while the HQ-treated skin darkened and returned to the pretreatment value. The dA treated sites showed no signs of skin irritation. In contrast, HQ treated skin showed signs of irritation that resulted in irregular darkening, beginning at 3 weeks of treatment. In contrast, kojic acid, and arbutin were not significantly different from the vehicle control.
文献2，Chawla S et al. Mechanism of tyrosinase inhibition by deoxyArbutin and its second-generation derivatives.. Br J Dermatol. 2008 Dec;159(6):1267-74. PMID:18811684

体内研究（细胞）：

细胞类型（Cell type）：Human melanocytes

药物配制（Preparation）：All compounds (dA, dF, fdA, tdA and HQ) were dissolved in sterile DMSO. Stock solutions of compounds were prepared and protected from light at -20℃until use. At time of use, compounds were further diluted in growth medium to produce the final required concentration. Compounds were tested at the appropriate concentration range (1-100μM) in triplicate in order to calculate LD50values.

实验方法（Assay）：Melanocytes were seeded at 1-3×105cells and allowed to grow for 48h before treatment. Cultured were treated daily with fresh growth media containing test compound for 5 days. On the sixth day, cells were detached and counted.

实验结果（Results）：A concentration-dependent decrease in the cell number of both was observed for all inhibitors (dA, dF, fdA, tdA and HQ). LD50concentrations of dA and its derivatives are listed below.

  LD50(dark melanocytes) LD50(light melanocytes)
dA (deoxyarbutin) 57.7±1.5 104.7±1.0
tdA (thideoxyarbutin) 48.0±2.0 87.8±2.5
fdA (fluorodeoxyarbutin) 89.1±0.7 106±1.5

相关产品

货号 名称 规格              
MZ95031-5G       Arbutin 熊果苷 5g
MZ95032-1G alpha-Arbutin alpha-熊果苷 1g
MZ95033-1G Deoxyarbutin 脱氧熊果苷 1g
MP0507-25KU Polyphenol Oxidase (Tyrosinase) 多酚氧化酶（酪氨酸酶）    25KU