标签归档：磷酸甘油

抗体/试剂/诊断抗体原料,3-磷酸甘油醛脱氢酶（内参）单克隆抗体 | MouseAnti-GAPDH-Loading Control antibody

发表于由

品牌：Bioss/博奥森 | 货号：bsm-33033M

产品编号 bsm-33033M
英文名称 GAPDH-Loading Control
中文名称 3-磷酸甘油醛脱氢酶（内参）单克隆抗体
别    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  新陈代谢  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 4F8
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit, Sheep, Hamster, Monkey, 
产品应用 WB=1:5000-20000 IHC-P=1:100-500 ICC=1:100 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 38kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinded Human GAPDH : 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer’s and Huntington’s disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting JP-glyceraldehyde 3-phosphate (G3P) into 3-phospho-JP-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

SWISS:
P04406

Gene ID:
2597

Database links:

Entrez Gene: 374193 Chicken

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: P00356 Chicken

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P04797 Rat

SwissProt: Q5XJ10 Zebrafish

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。
GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。
甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。
GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.

产品图片
Sample:
Lane1: Skin (Mouse) Lysate at 40 ug
Lane2: Testis (Mouse) Lysate at 40 ug
Lane3: Adrenal gland (Mouse) Lysate at 40 ug
Lane4: Lung (Rat) Lysate at 30 ug
Primary: Anti-GAPDH (bsm-33033M) at 1/1 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD

Sample:
A549 Cell Lysate at 25 ug
293T Cell Lysate at 40 ug
Primary: Anti-GAPDH(bsm-33033M)at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD

Sample:
H9C2 Cell (Rat) Lysate at 40 ug
U87MG Cell (Human) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Primary: Anti- GAPDH (bsm-33033M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 35 kD

Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Primary:
Lane 1: Anti-GAPDH (bsm-33033M) at 1/2000 dilution
Lane 2: Anti-GAPDH (bsm-33033M) at 1/5000 dilution
Lane 3: Anti-GAPDH (bsm-33033M) at 1/10000 dilution
Lane 4: Anti-GAPDH (bsm-33033M) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 36 kD

Sample:
Lane 1: Hela (Human) Lysate at 40 ug
Lane 2: Hela (Human) Lysate at 40 ug
Lane 3: Hela (Human) Lysate at 40 ug
Lane 4: Hela (Human) Lysate at 40 ug
Primary:
Lane 1: Anti-GAPDH (bsm-33033M) at 1/2000 dilution
Lane 2: Anti-GAPDH (bsm-33033M) at 1/5000 dilution
Lane 3: Anti-GAPDH (bsm-33033M) at 1/10000 dilution
Lane 4: Anti-GAPDH (bsm-33033M) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 36 kD

Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Testis (Mouse) Lysate at 40 ug
Lane 6: Testis (Rat) Lysate at 40 ug
Lane 7: Kidney (Mouse) Lysate at 40 ug
Lane 8: HUVEC (Human) Cell Lysate at 30 ug
Lane 9: A549 (Human) Cell Lysate at 30 ug
Lane 10: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (bsm-33033M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti- GAPDH (bsm-33033M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 35 kD

Sample:
Hyperpituitarism (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (bsm-33033M) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 34 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH-Loading Control) monoclonal Antibody, Unconjugated (bsm-33033M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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抗体/试剂/诊断抗体原料,3-磷酸甘油醛脱氢酶（内参）抗体 | RabbitAnti-GAPDH (Loading Control) antibody

发表于由

品牌：Bioss/博奥森 | 货号：JP-2188R

产品编号 JP-2188R
英文名称 GAPDH (Loading Control)
中文名称 3-磷酸甘油醛脱氢酶（内参）抗体
别    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
Specific References  (57)     |     JP-2188R has been referenced in 57 publications.
[IF=0] Zhang et al. Increased stathmin expression strengthens fear conditioning in epileptic rats. (2015) Biomed.Rep. 3:28-32  WB ;  Rat.  
PubMed:25469242

[IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82.  WB ;  Rabbit.  
PubMed:31145948

[IF=7.901] Zhang N et al. Polypeptide-engineered DNA tetrahedrons for targeting treatment of colorectal cancer via apoptosis and autophagy. J Control Release. 2019 Jul 10;309:48-58.   WB ;  Human.  
PubMed:31301339

[IF=6.306] Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.  WB ;  Rat.  
PubMed:30408497

[IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  WB ;  Mouse.  
PubMed:30791957

[IF=5.79] Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66  WB ;  Human.  
PubMed:26112597

[IF=5.17] Zhang et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. (2018) Oncotarget. 9:2175-2192  WB ;  Rat.  
PubMed:29416763

[IF=5.005] Li Y et al. Exosomes derived from Toxoplasma gondii stimulate an inflammatory response through JNK signaling pathway. Nanomedicine (Lond). 2018 May;13(10):1157-1168.  WB ;  Human.  
PubMed:29542367

[IF=4.46] Yan, Lijie, et al. “Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy.” Journal of cardiovascular pharmacology 64.4 (2014): 310-317.  WB ;  Human.  
PubMed:24887685

[IF=4.38] Sun, Pengchao, et al. “SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer.” International Journal of Nanomedicine 12 (2017): 2657.  WB ;  Human.  
PubMed:28435250

[IF=4.258] Steenblock et al. The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts. (2014) J.Biol.Che. 289:18347-59  WB ;  Mouse.  
PubMed:24821726

[IF=3.974] Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119.  WB ;  Chicken.  
PubMed:30822667

[IF=3.895] Shen X et al.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.(2018) Cell Mol Neurobiol.   WB ;  Human.  
PubMed:30554401

[IF=3.831] Zhang H et al. Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway.Front Pharmacol. 2019 Mar 5;10:175.   WB ;  Rat.  
PubMed:30890937

[IF=3.687] Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10.   WB ;  Rat.  
PubMed:30972748

[IF=3.562] Zhang,et al.Interferon-γ Promotes Neuronal Repair by Transplanted Neural Stem Cells in Ischemic Rats.(2018) Stem Cells and Development. 27:355-366.  WB ;  Rat.  
PubMed:29298609

[IF=3.457] Ling L et al. MicroRNA-30e promotes hepatocyte proliferation and inhibits apoptosis in cecal ligation and puncture-induced sepsis through the JAK/STAT signaling pathway by binding to FOSL2.Biomed Pharmacother. 2018 Aug;104:411-419.  WB ;  Rat.  
PubMed:29787988

[IF=3.44] Chen B et al. Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.(2018) Brain Res Bull.  WB ;  Mouse.  
PubMed:30593877

[IF=3.412] Yang Q et al. Activation of Nrf2 by phloretin attenuates palmitic acid-induced endothelial cell oxidative stress via AMPK-dependent signaling.(2018) J Agric Food Chem.   WB ;  Human.  
PubMed:30525573

[IF=3.205] Liu et al. Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala. (2018) Mol.Pain. 14:1744806918805902  WB ;  Mouse.  
PubMed:30232930

[IF=3.144] Liu,et al.Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates.(2018) Journal of Functional Foods. 42:203-215.  WB ;  Human.  
PubMed:10.1016/j.jff.2017.12.003

[IF=3.04] Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49.   WB ;  Human.  
PubMed:30707959

[IF=2.78] Chai et al. Hypoxia induces pulmonary arterial fibroblast proliferation, migration, differentiation and vascular remodeling via the PI3K/Akt/p70S6K signaling pathway. (2018) Int.J.Mol.Med. 41:2461-2472  IF ;  Rat.  
PubMed:29436587

[IF=2.669] Qiu,et al.Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway.(2018) Molecular and Cellular Biochemistry. :.  WB ;  Human.  
PubMed:29363056

[IF=2.634] Zhang X et al. BMP9 Promotes the Extracellular Matrix of Nucleus Pulposus Cells Via Inhibition of the NotchSignaling Pathway. DNA Cell Biol. 2019 Feb 13.  WB ;  Human.  
PubMed:30758228

[IF=2.559] Qian L et al. Upregulated circular RNA circ_0074027 promotes glioblastoma cell growth and invasion by regulating miR-518a-5p/IL17RD signaling pathway. Biochem Biophys Res Commun. 2019 Mar 19;510(4):515-519.   WB ;  Human.  
PubMed:30738578

[IF=2.498] Huang H et al. Molecular characterization, expression and cellular localization of CYP17 gene during geese (Anser cygnoides) follicular development. Gene. 2018 Jun 5;658:184-190.   WB ;  Geese.  
PubMed:29544766

[IF=2.49] Wang, Hong, et al. “Osthole shows the potential to overcome P-glycoprotein‑mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway.” Oncology reports 35.6 (2016): 3659-3668.  WB ;  Human.  
PubMed:27109742

[IF=2.431] Wang L et al. Serious Selenium Deficiency in the Serum of Patients with Kashin–Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes. Biol Trace Elem Res. 2019 Jun 8.   WB ;  Human.  
PubMed:31175635

[IF=2.387] Jiang L et al. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression. Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):3012-3020.   WB ;  Rat.  
PubMed:31002152

[IF=2.314] Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757.  WB ;  Mouse.  
PubMed:30739356

[IF=2.3] Zhang, Xiangliang, et al. “MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy.” Oncology Reports.  WB ;  
PubMed:26134156

[IF=2.14] Song, Yong, et al. “Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis.” Reproductive Biology and Endocrinology 12.1 (2014): 42.  WB ;  Human.  
PubMed:24884521

[IF=2.11] Yan, Lijie, et al. “Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy.” Journal of cardiovascular pharmacology 64.4 (2014): 310-317.  WB ;  Human.  
PubMed:24887685

[IF=2.11] Li et al. All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts. (2010) Mol.Vi. 16:689-97  WB ;  Human.  
PubMed:20405022

[IF=2.09] Tan, Shifan, et al. “miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy.” International Journal of Molecular Medicine.  WB ;  
PubMed:26935807

[IF=1.922] Cao et al. Dickkopf‑3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257  WB ;  rabbit.  
PubMed:29568962

[IF=1.92] Huang et al. Intracellular iodinated compounds affect sodium iodide symporter expression through TSH-mediated signaling pathways. (2011) Mol.Med.Rep. 4:77-80  WB ;  Porcine.  
PubMed:21461567

[IF=1.851] Dai X et al. Silencing of MALAT1 inhibits migration and invasion by sponging miR‑1‑3p in prostate cancer cells. Mol Med Rep. 2019 Aug 22.   WB ;  Human.  
PubMed:31485645

[IF=1.632] Wang R et al. Molecular cloning and functional characterization of porcine 2′,5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol. 2019 Mar;209:22-30.  WB ;  Porcine.  
PubMed:30885302

[IF=1.563] Wang,et al.Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules.() Nephrology. :.  WB ;  Rat.  
PubMed:29280536

[IF=1.55] Jia, Lianqun, et al. “Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats.” Molecular Medicine Reports (2016).  WB ;  Rat.  
PubMed:27082100

[IF=1.514] Wang Y et al. Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress. Iran J Basic Med Sci. 2018 Dec;21(12):1238-1244.  WB ;  Rat.  
PubMed:30627367

[IF=1.48] Hou, Yu Sen, et al. “Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein.” Molecular Medicine Reports.  WB ;  Human.  
PubMed:25955291

[IF=1.358] Ma WF et al. Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase signaling pathway. Journal of Food Biochemistry,2019 e12893.   WB ;  Human.  
PubMed:doi:10.1111/jfbc.12893

[IF=1.28] YIN, Zhi-hong, et al. “Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors.” Journal of Zhejiang University Science B 1 (2016).  WB ;  Sheep.  
PubMed:0

[IF=1.189] Xu Q et al. Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 May 18;12(5):711-716.   WB ;  Rat&Human.  
PubMed:31131227

[IF=.99] Shao, Guangcan, et al. “Construction of the plasmid coding for the expression of the EGFP‑M‑IL‑2 (88Arg, 125Ala) fusion protein and the anti‑tumor effects exerted by the fusion protein in HeLa-60 cells.” Oncology Letters.  WB ;  Human.  
PubMed:26137137

[IF=0] Kaushal P et al. Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum. Journal of Ayurveda and Integrative Medicine.2019  WB ;  Rat.  
PubMed:doi:10.1016/j.jaim.2018.02.138 

[IF=0] Wen Y et al. NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway. Oxid Med Cell Longev. 2019 May 9;2019:4578175.  WB ;  Rat.  
PubMed:31210840

[IF=0] Jiang S et al. Inhibitory Effect of Slit2-N on VEGF165-induced proliferation of vascular endothelia via Slit2-N-Robo4-Akt pathway in choroidal neovascularization. Cell Cycle. 2019 Jun;18(11):1241-1253.   WB ;  Human.  
PubMed:31081721

[IF=] Li T et al. Withanolides, extracted from Datura metel L. inhibit keratinocyte proliferation and imiquimod- induced psoriasis-like dermatitis via the STAT3/P38/ERK1/2 pathway. Molecules. 2019 Jul 17;24(14). pii: E2596.   WB ;  Human.  
PubMed:31319488

[IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692.   WB ;  Human.  
PubMed:31560411

[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.  WB ;  Mouse.  
PubMed:31497217

[IF=2.926] Wang M et al. Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconj J. 2019 Sep 2.   WB ;  Human.  
PubMed:31478096

[IF=1.851] Duan X et al. Expression of TIGIT/CD155 and correlations with clinical pathological features in human hepatocellular carcinoma. Mol Med Rep. 2019 Oct;20(4):3773-3781.   WB ;  Human.  
PubMed:31485637

[IF=.607] Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb.  WB ;  Chicken.  
PubMed:doi:10.1590/1806-9061-2018-0835

产品类型 内参抗体 
研究领域 免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 WB=1:2000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 37kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 GAPDH protein of rabbit: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer’s and Huntington’s disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting JP-glyceraldehyde 3-phosphate (G3P) into 3-phospho-JP-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

SWISS:
P04406

Gene ID:
100009074

Database links:

Entrez Gene: 493876 Cat

Entrez Gene: 374193 Chicken

Entrez Gene: 403755 Dog

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 396823 Pig

Entrez Gene: 100009074 Rabbit

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 380259 Xenopus laevis

Entrez Gene: 448356 Xenopus tropicalis

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: Cat

SwissProt: P00356 Chicken

SwissProt: Q28259 Dog

SwissProt: P70685 Guinea pig

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P00355 Pig

SwissProt: P46406 Rabbit

SwissProt: P04797 Rat

SwissProt: P51469 Xenopus laevis

SwissProt: Q5XJ10 Zebrafish

Unigene: 544577 Human

Unigene: 592355 Human

Unigene: 598320 Human

Unigene: 304088 Mouse

Unigene: 309092 Mouse

Unigene: 317779 Mouse

Unigene: 343110 Mouse

Unigene: 392463 Mouse

Unigene: 392480 Mouse

Unigene: 414470 Mouse

Unigene: 458138 Mouse

Unigene: 458416 Mouse

Unigene: 475698 Mouse

Unigene: 129558 Rat

Unigene: 91450 Rat

Unigene: 995 Xenopus laevis

Unigene: 35640 Zebrafish

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。 GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成。

产品图片
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Hela Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- GAPDH (JP-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
A549(Human) Cell Lysate at 30 ug
U2os(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-2188R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Heart (Rat) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (JP-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD

Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-GAPDH (JP-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH (JP-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD

Sample:
Muscle (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti- GAPDH (JP-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Lane 1: NIH/3T3(Mouse) Cell Lysate at 30 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: HUVEC (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Lane 6: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
U251(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (JP-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (JP-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(JP-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(JP-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

本网站可提供的所有产品和服务均不得用于人体或动物的临床诊断或治疗，仅可用于科研等非医疗目的。

抗体/试剂/诊断抗体原料,3-磷酸甘油醛脱氢酶（内参）抗体 | RabbitAnti-GAPDH (Loading Control) antibody

发表于由

品牌：Bioss/博奥森 | 货号：JP-10900R

产品编号 JP-10900R
英文名称 GAPDH (Loading Control)
中文名称 3-磷酸甘油醛脱氢酶（内参）抗体
别    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
Specific References  (48)     |     JP-10900R has been referenced in 48 publications.
111 [IF=1.39] Wang, Zhi‑Liang, et al. “Irreversible electroporation‑mediated shRNA knockdown of the HPV18 E6 gene suppresses cervical cancer growth in vitro and in vivo.” Oncology Letters.  WB ;  Human.  222
PubMed:28781638

111 [IF=5.008] Liang et al. Itraconazole exerts its anti-melanoma effect by suppressing Hedgehog, Wnt, and PI3K/mTOR signaling pathways. (2017) Oncotarge. 8:28510-28525  WB ;  Human.  222
PubMed:28212537

111 [IF=5.008] Mu et al. Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/β-catenin signaling and is frequently methylated in breast cancer. (2017) Oncotarge. 8:39443-39459  WB ;  Human.  222
PubMed:28467796

111 [IF=4.652] Zong et al. The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro. (2016) Cell.Physiol.Bioche. 38:1100-10  WB ;  Mouse.  222
PubMed:26938714

111 [IF=4.652] Lin et al. MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells. (2017) Cell.Physiol.Bioche. 41:124-136  WB ;  Rat.  222
PubMed:28214900

111 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458  WB ;  Human.  222
PubMed:26881030

111 [IF=2.272] Yang et al. Lrig1 is a positive prognostic marker in hepatocellular carcinoma. (2016) Onco.Targets.The. 9:7071-7079  WB ;  Human.  222
PubMed:27895499

111 [IF=2.137] Yin et al. Expression and Clinical Significance of ILF2 in Gastric Cancer. (2017) Dis.Marker. 2017:4387081  WB ;  Human.  222
PubMed:28831206

111 [IF=1.39] Guo et al. BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition. (2014) Oncol.Let. 8:869-875  WB ;  Human.  222
PubMed:25013510

111 [IF=1.39] Wang et al. BRAF-activated long non-coding RNA contributes to cell proliferation and activates autophagy in papillary thyroid carcinoma. (2014) Oncol.Let. 8:1947-1952  WB ;  Human.  222
PubMed:25289082

111 [IF=1.238] Liu and Xiao Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia. (2017) BMC.Ophthalmo. 17:135  WB ;  Human.  222
PubMed:28764685

111 [IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344  WB ;  Human.  222
PubMed:29512784

111 [IF=6.34] Zhou et al. Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression. (2017) Redox.Biol. 11:390-402  WB ;  Rat, Human.  222
PubMed:28063381

111 [IF=1.77] Duan et al. Antidepressant effect of electroacupuncture regulates signal targeting in the brain and increases brain-derived neurotrophic factor levels. (2016) Neural.Regen.Res. 11:1595-1602  WB ;  Rat.  222
PubMed:27904490

111 [IF=4.302] Jia et al. Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α. (2018) J.Cell.Mol.Med. 22:4507-4521  WB ;  Mouse.  222
PubMed:29993193

111 [IF=2.784] Yang et al. miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. (2018) Int.J.Mol.Med. 42:3115-3124  WB ;  
PubMed:30272255

111 [IF=2.766] Wan et al. Dietary protein-induced hepatic IGF-1 secretion mediated by PPARγ activation. (2017) PLoS.One. 12:e0173174  WB ;  Pig.  222
PubMed:28257428

111 [IF=1.492] Song et al. Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression. (2017) Braz.J.Med.Biol.Res. 50:e5407  WB ;  Rat.  222
PubMed:28678913

111 [IF=1.936] Yu B et al. Inhibition of microRNA-143-3p attenuates myocardial hypertrophy by inhibiting inflammatory response. (2018) Nov;42(11):1584-1593.  WB ;  (SD) Rats.  222
PubMed:30203887

111 [IF=2.976] Guo H et al. Clinical associations between ASCT2 and p-mTOR in the pathogenesis and prognosis of epithelial ovarian cancer. Oncol Rep. 2018 Dec;40(6):3725-3733.  WB ;  Human.  222
PubMed:30272366

111 [IF=3.448] Liu X et al. Lysosomal dysfunction is associated with persistent lung injury in dams caused by pregnancy exposure to carbon black nanoparticles. Life Sci. 2019 Sep 15;233:116741.  WB ;  Mouse.  222
PubMed:31398419

111 [IF=2.675] Liu X et al. The lysosomal membrane protein LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy. FEBS Lett. 2019 Nov 6.  WB ;  Human.  222
PubMed:31693752

111 [IF=2.35] Yu B et al. Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Exp Mol Pathol. 2019 Nov 21;112:104346.  WB ;  Rat.  222
PubMed:31758917

111 [IF=3.598] Chang L et al. Gypenoside A protects ischemia/reperfusion injuries by suppressing miR‐143‐3p level via the activation of AMPK/Foxo1 pathway. Biofactors. 2019 Dec 30.  WB ;  Rat.  222
PubMed:31889343

111 [IF=1.851] Wang T et al. The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway. Mol Med Rep. 2020 Mar;21(3):1097-1106.  WB ;  Rat.  222
PubMed:32016456

111 [IF=4.784] Liu XQ et al. Sodium tanshinone IIA sulfonate protects against Aβ1–42-induced cellular toxicity by modulating Aβ-degrading enzymes in HT22 cells. Int J Biol Macromol. 2020 Feb 6;151:47-55.  WB ;  Mouse.  222
PubMed:32035959

111 [IF=3.448] Wang T et al. Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats. Life Sci. 2020 Feb 13;247:117421.  WB ;  Rat.  222
PubMed:32061865

111 [IF=.578] Shen D et al. Efficacy evaluation and mechanism study on inhibition of breast cancer cell growth by multimodal targeted fluorescent nanobubbles carrying AMD070 and ICG. Nanotechnology. 2020 Mar 10;31(24):245102.  WB ;  human.  222
PubMed:32155591

111 [IF=4.268] Wu H et al. A UPLC-Q-TOF/MS-based plasma metabolomics approach reveals the mechanism of Compound Kushen Injection-based intervention against non-small cell lung cancer in Lewis tumor-bearing mice. Phytomedicine . 2020 Jun 2;76:153259.  WB ;  Mouse.  222
PubMed:32534358

111 [IF=3.647] Lin X et al. Sumoylation enhances the activity of the TGF-β/SMAD and HIF-1 signaling pathways in keloids. Life Sci . 2020 Aug 15;255:117859.  WB ;  Human.  222
PubMed:32474020

产品类型 内参抗体 
研究领域 肿瘤  细胞生物  免疫学  信号转导  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:10000-200000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 38kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant human GAPDH full length protein 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer’s and Huntington’s disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting JP-glyceraldehyde 3-phosphate (G3P) into 3-phospho-JP-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

SWISS:
P04406

Gene ID:
2597

Database links:

Entrez Gene: 374193 Chicken

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: P00356 Chicken

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P04797 Rat

SwissProt: Q5XJ10 Zebrafish

GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。
GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。
甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。
GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.

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Sample: 293T(human) cell lysate at 30ug;
Primary:
Lane1: Anti-GAPDH (JP-10900R) at 1/2000 dilution
Lane2: Anti-GAPDH (JP-10900R) at 1/10000 dilution
Lane3: Anti-GAPDH (JP-10900R) at 1/40000 dilution
Lane4: Anti-GAPDH (JP-10900R) at 1/80000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38kD

Sample:
Lane 1: SiHa (Human) Cell Lysate at 30 ug
Lane 2: NIH/3T3(Mouse) Cell Lysate at 30 ug
Lane 3: Large intestine (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Cerebrum (Mouse) Lysate at 40 ug
Lane 6: Testis (Rat) Lysate at 40 ug
Lane 7: Testis (Mouse) Lysate at 40 ug
Lane 8: Kidney (Mouse) Lysate at 40 ug
Lane 9: HUVEC (Human) Cell Lysate at 30 ug
Lane 10: A549 (Human) Cell Lysate at 30 ug
Lane 11: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-10900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Sample:
293T (Human) Lysate at 40 ug
Primary:
Anti-GAPDH (JP-10900R) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 36 kD

Sample:
H9C2(Rat) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-10900R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD

Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (JP-10900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD

Sample:
A549 Cell (Human) Lysate at 40 ug
A431 Cell (Human) Lysate at 40 ug
NIH/3T3 Cell (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH (JP-10900R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 36 kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Contro) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (JP-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH (Loading Control)) polyclonal Antibody, Unconjugated (JP-10900R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH (Loading Control)) polyclonal Antibody, Unconjugated (JP-10900R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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