描述
Puromycin Aminonucleoside 嘌呤霉素氨基核苷
产品标签
Puromycin Aminonucleoside 嘌呤霉素氨基核苷；Puromycin 嘌呤霉素盐酸盐；Nephrotic syndrome 肾病综合征；Proteinuria 蛋白尿症；CAS：58-60-6；
产品信息
| 产品名称 | 产品编号 | 规格 | 价格（元） |
| Puromycin Aminonucleoside 嘌呤霉素氨基核苷 | MS0124-10MG | 10mg | 896 |
| Puromycin Aminonucleoside 嘌呤霉素氨基核苷 | MS0124-50MG | 50mg | 3566 |
产品描述
嘌呤霉素氨基核苷（Puromycin Aminonucleoside, PANS）是抗生素嘌呤霉素的氨基核苷部分，这一嘌呤霉素类似物不能抑制蛋白合成或诱导凋亡，替代的是用于诱导肾病的实验动物模型，以研究局灶节段性肾小球硬化、肾病变的诱导和引发蛋白尿症的事件。大鼠体内注射PANS（0.5-5 μM）能影响肾小球上皮细胞黏附，降低α3β1整合素表达。PANS还能减少肾病蛋白（Nephrin）和足突蛋白（Podocin）的表达。
产品特性
1） 化学名：4-amino-2-[6-(dimethylamino)purin-9-yl]-5-(hydroxymethyl)oxolan-3-ol
2） 同义名：PANS; NSC 3056;3′-amino-3′-deoxy-N,N-dimethyl-adenosine; 3′-Amino-3′-deoxy-N6,N6- dimethyladenosine; 氨基甘嘌呤霉素；氨基核苷嘌呤霉素；
3） CAS NO：58-60-6
4） 来源：半合成，来自嘌呤霉素
5） 分子式：C12H18N6O3
6） 分子量：294.3 g/mol
7） 纯度：≥98% (HPLC)
8） 溶解性：溶于DMSO（20mg/ml），DMF（10mg/ml），H2O（10mg/ml）
9） 化学结构式：
保存与运输方法
保存：-20℃干燥保存，3年有效。
运输：室温运输。
配制储存液
| 质量
溶剂体积 浓度 |
1mg | 5mg | 10mg | 50mg |
| 1mM | 3.3978 mL | 16.9889 mL | 33.9778 mL | 169.8889 mL |
| 5mM | 0.6796 mL | 3.3978 mL | 6.7956 mL | 33.97778 mL |
| 10mM | 0.3398 mL | 1.6989 mL | 3.3978 mL | 16.9889mL |
| 50mM | 0.0680 mL | 0.3398 mL | 0.6796 mL | 3.3978 mL |
使用方法【源自文献，仅作参考】
| 文献1，Krishnamurti U et al.Puromycin aminonucleoside suppresses integrin expression in cultured glomerular epithelial cells.J Am Soc Nephrol. 2001 Apr;12(4):758-66. PMID: 11274237
体外研究： 细胞类型（Cell type）：T- SV40 immortalized human glomerular epithelial cell line (56/10 A1) 实验方法（Assay）：The 56/10 A1 cells were seeded at 0.01 × 106/cm2in 96-well plates and incubated for 2 d at 37℃. PAN was added to the media at a concentration of 0, 0.5, or 5.0 μg/ml (n = 12 for each PAN concentration), and the cells were incubated for another 48 h. Then the effect of PAN on proliferation of cells was assessed by an enzyme-linked immunosorbent assay method, using BrdU. 实验结果（Results）：Control wells that were not exposed to PAN contained 204.3 ± 31.1 × 103cells/cm2, compared with 204.0 ± 30.6 × 103cells/cm2in wells that were exposed to 0.5 μg/ml PAN and 163.7 ± 36.3 × 103cells/cm2in wells that were exposed to 5.0 μg/ml PAN. |
| 文献2，Kho MC et al. Plantago asiatica L. Ameliorates Puromycin Aminonucleoside-Induced Nephrotic Syndrome by Suppressing Inflammation and Apoptosis. Nutrients. 2017 Apr 14; 9(4). pii: E386. PMID: 28420111
体内研究： 动物模型（Animal Model）：Eight-week-old maleSprague-Dawley (SD) rats (weighing170–200g) 药物配制（Preparation）：Puromycin aminonucleoside (PAN) was dissolved in dissolved in saline 实验方法（Assays）：Rats were randomly divided into four groups: Control group; a PAN-treated Nephrotic syndrome (NS) group; a NS plus Losartan (30 mg/kg/day) treated group; and a NS plus PAL (200 mg/kg/day) treated group. Rats were intravenously injected with PAN (75 mg/kg/day, dissolved in saline). The control group was injected with saline only. Rats were administered either distilled water (Control and NS groups), Losartan or PAL by an oral gavage method once daily for 7 days. 实验结果（Results）：Proteinuria is an important characteristic of puromycin aminonucleoside-induced NS in rats. 24-h urinary protein excretion progressively increased following PAN injection. Proteinuria levels in the NS group peaked on day 7 compared to the Control group. However, the PAL-treated group exhibited significantly decreased proteinuria levels on day 7 (p < 0.01). Similarly, treatment with losartan produced similar results to those seen in the PAL group. 给药途径（Administration）：Intravenous (i.v.) injection |