描述
pBARGPE1-EGFP Vector 真菌表达载体
产品信息：
|
货号 |
产品名称 |
规格 |
价格（元） |
|
MF3400-2UG |
pBARGPE1-EGFP Vector 真菌表达载体 |
2μg |
1800 |
基本信息
| 载体用途： | 丝状真菌表达载体（质粒） |
| 载体大小： | 6226bp |
| 原核抗性： | Amp |
| 克隆菌株： | DH5α |
| 荧光标记： | EGFP（绿色荧光） |
| 启动子： | gpdA, TrpC |
| 复制子： | pUC ori |
| 终止子： | trpC |
| 表达宿主： | 丝状真菌 |
| 真核抗性： | Bar |
| 诱导方式： | 无需诱导 |
| 5′ 测序引物： | 根据全序列设计引物 |
| 3′ 测序引物： | 根据全序列设计引物 |
质粒图谱
保存与运输方法
保存：-20℃保存，至少1年有效。
运输：冰袋运输。
注意事项
1） 我司提供的载体/质粒/菌株都经过严格的质量控制。请收到货后先进行转化，再挑取单克隆扩增。产品使用中若遇到质量问题，请和我司及时沟通。
2） 为了您的安全和健康，请穿实验服并戴一次性手套操作。
质粒序列
LOCUS Exported 6226 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBARGPE1-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6226)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6226
/organism=”synthetic DNA construct”
/mol_type=”other DNA”
primer_bind 18..34
/note=”KS primer”
/note=”common sequencing primer, one of multiple similar
variants”
CDS complement(55..774)
/codon_start=1
/product=”enhanced GFP”
/note=”EGFP”
/note=”mammalian codon-optimized”
/translation=”MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK”
primer_bind 789..805
/note=”KS primer”
/note=”common sequencing primer, one of multiple similar
variants”
misc_feature complement(855..1539)
/note=”gpdA Promoter”
misc_feature 1754..1894
/note=”bom”
/note=”basis of mobility region from pBR322″
rep_origin complement(2080..2668)
/direction=LEFT
/note=”ori”
/note=”high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication”
CDS complement(2839..3699)
/codon_start=1
/gene=”bla”
/product=”beta-lactamase”
/note=”AmpR”
/note=”confers resistance to ampicillin, carbenicillin, and
related antibiotics”
/translation=”MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW”
promoter complement(3700..3804)
/gene=”bla”
/note=”AmpR promoter”
promoter 3910..4011
/gene=”S. cerevisiae TRP1″
/note=”TRP1 promoter”
misc_feature 4102..4487
/note=”TrpC Promotor”
CDS 4488..5039
/codon_start=1
/gene=”Streptomyces hygroscopicus bar”
/product=”phosphinothricin acetyltransferase”
/note=”Bar”
/note=”confers resistance to bialophos or phosphinothricin”
/translation=”MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI”
protein_bind 5431..5464
/bound_moiety=”Cre recombinase”
/note=”loxP”
/note=”Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).”
protein_bind 5542..5575
/bound_moiety=”Cre recombinase”
/note=”loxP”
/note=”Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).”
misc_feature 5969..6199
/note=”trpC terminator”
ORIGIN
1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcttactt
61 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
121 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
181 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
241 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
301 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
361 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
421 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
481 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
541 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
601 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
661 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
721 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggatcc
781 tctagagtcg aggtcgacgg tatcgataag cttgattaaa ggttcttgga tgggaagatg
841 aatatactga agatgggaaa agaaagagaa aagaaaagag cagctggtgg ggagagcagg
901 aaaatatggc aacaaatgtt ggactgacgc aacgaccttg tcaaccccgc cgacacaccg
961 ggcggacaga cggggcaaag ctgcctacca gggactgagg gacctcagca ggtcgagtgc
1021 agagcaccgg atgggtcgac tgccagcttg tgttcccggt ctgcgccgct ggccagctcc
1081 tgagcggcct ttccggtttc atacaccggg caaagcagga gaggcacgat atttggacgc
1141 cctacagatg ccggatgggc caattaggga gcttacgcgc cgggtactcg ctctacctac
1201 ttcggagaag gtactatctc gtgaatcttt taccagatcg gaagcaattg gacttctgta
1261 cctaggttaa tggcatgcta tttcgccgac ggctatacac ccctggcttc acattctcct
1321 tcgcttactg ccggtgattc gatgaagctc catattctcc gatgatgcaa tagattcttg
1381 gtcaacgagg ggcacaccag cctttccact tcggggcgga ggggcggccg gtcccggatt
1441 aataatcatc cactgcacct cagagccgcc agagctgtct ggcgcagtgg cgcttattac
1501 tcagcccttc tctctgcgtc cgtccgtctc tccgcatgct ttgcatactt ctgcctgctg
1561 gggagcctgg ggactttcca caccctaact gacacacatt ccacagagat ctctgcctcg
1621 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
1681 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
1741 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
1801 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
1861 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
1921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
1981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
2041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
2101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
2161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
2221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
2281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
2341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
2401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
2461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
2521 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
2581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
2641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
2701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
2761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
2821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
2881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
2941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
3001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
3061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
3121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
3181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
3241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
3301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
3361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
3421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
3481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
3541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
3601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
3661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
3721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
3781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
3841 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
3901 tcaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
3961 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
4021 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
4081 gccgcagaat gtgctctaga ctcgacagaa gatgatattg aaggagcact ttttgggctt
4141 ggctggagct agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg
4201 agcacaaaat ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc
4261 cacttgtagc agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg
4321 acattattat catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa
4381 gtgaacgacc cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat
4441 ctgacttacc tattctaccc aagcaaagct tcgattagga agtaaccatg agcccagaac
4501 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
4561 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
4621 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
4681 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
4741 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
4801 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
4861 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
4921 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
4981 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgat
5041 ccgtcgacct gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
5101 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
5161 taacatgtaa tgcatagtac cgagaaacta gtggatctaa acataaaatc tgtaaaataa
5221 caagatgtaa agataatgct aaatcatttg gctttttgat tgattgtaca ggaaaatata
5281 catcgcaggg ggttgacttt taccatttca ccgcaatgga atcaaacttg ttgaagagaa
5341 tgttcacagg cgcatacgct acaatgaccc gattcttgct agccttttct cggtcttgca
5401 aacaaccgcc gcggccggtc gagggaccta ataacttcgt atagcataca ttatacgaag
5461 ttatattaag ggttccggat cgcggccgct cgacctgcag ccaagctagc ttggctggac
5521 gtaaactcct cttcagacct aataacttcg tatagcatac attatacgaa gttatattaa
5581 gggttattga atatgatcgg aattggtcga tcaggtattg ctgttatctg atgagtatac
5641 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac
5701 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt
5761 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc ttgcaaatta
5821 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
5881 acacgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
5941 taaaaaccca tctcataaat aacgtcatgc attgcagatg agctgtatct ggaagaggta
6001 aacccgaaac gcgttttatt cttgttgaca tggagctatt aaatcactag aaggcactct
6061 ttgctgcttg gacaaatgaa cgtatcttat cgagatcctg aacaccattt gtctcaactc
6121 cggagctgac atcgacacca acgatcttat atccagattc gtcaagctgt ttgatgattt
6181 cagtaacgtt aagtggatct cggtgacggg caggaccgga cggggc