描述
SD/-Trp/-His/-Ade with Agar
产品标签
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | 价格（元） |
| SD/-Trp/-His/-Ade with Agar 酵母缺陷型培养基（琼脂） | MS6422-5L | 10×0.5 L | 1198 |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Trp/-His/-Ade with Agar就是缺乏色氨酸（Trp），组氨酸（His）和腺嘌呤（Ade）三种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |