–安培瓶：玻璃、阔口
–容量：2 mL
–靶标微生物：粪便大肠菌
–25 oC时pH值：7.4 ± 0.2
–目标菌落颜色：蓝色
–培养基颜色：蓝色
–包装：20个/包装
标签归档：培养基
SD/-His/-Leu/-Trp/-Ura with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 规格 | ||
| SD/-His/-Leu/-Trp/-Ura with Agar 酵母缺陷型培养基（琼脂） | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-His/-Leu/-Trp/-Ura with Agar就是缺乏组氨酸（His），亮氨酸（Leu），色氨酸（Trp）和尿嘧啶（Ura）四种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和双/三杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-His/-Leu/-Met/-Trp with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 规格 | ||
| SD/-His/-Leu/-Met/-Trp with Agar 酵母缺陷型培养基（琼脂） |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD-His-Leu-Met-Trp with Agar就是缺乏组氨酸（His），亮氨酸（Leu），甲硫氨酸（Met）和色氨酸（Trp）四种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和双/三杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-His/-Leu/-Met/-Trp Broth 酵母缺陷型培养基（肉汤）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 规格 | ||
| SD/-Ade/-His/-Leu/-Trp Broth 酵母缺陷型培养基（肉汤） |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD-Ade-His-Leu-Trp Broth就是缺乏腺嘌呤（Ade），组氨酸（His），亮氨酸（Leu）和色氨酸（Trp）四种成分的酵母缺陷型液体培养基，主要用于双质粒酵母转化筛选和双/三杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 称取28g粉末加入1L去离子水，搅拌混匀使其溶解。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Ade/-His/-Leu/-Trp with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 规格 | ||
| SD/-Ade/-His/-Leu/-Trp with Agar 酵母缺陷型培养基（琼脂） |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Ade/-His/-Leu/-Trp with Agar就是缺乏腺嘌呤（Ade），组氨酸（His），亮氨酸（Leu）和色氨酸（Trp）四种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和双/三杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
Salt Czapek Dox Agar 高盐察氏培养基琼脂、高盐察氏琼脂培养基、高盐查氏培养基
高盐察氏培养基（Salt Czapek Dox Agar），又称为高盐察氏琼脂、高盐察氏琼脂培养基、高盐查氏培养基，用于霉菌和酵母菌的计数。以硝酸钠为唯一氮源，葡萄糖为碳源。磷酸氢二钾提供缓冲力，硫酸镁、氯化钾、硫酸亚铁提供必需的离子。高浓度的氯化钠减缓细菌生长速度。琼脂用作凝固剂。
产品成分（g/L）
|
磷酸氢二钾（Dipotassium phosphate）：1.0g |
硝酸钠（Sodium Nitrate）：2.0g |
|
氯化钾（Potassium Chloride）：0.5g |
硫酸镁（Magnesium Sulfate）：0.5g |
| 硫酸亚铁（Ferrous Sulfate）：0.01g |
蔗糖（Sucrose）：30.0g |
| 氯化钠（Sodium Chloride）：60.0g |
琼脂（Agar）：15.0g |
产品级别
微生物培养用(For microbiology)
产品用法
称取本品109.0g，加入1L蒸馏水（或去离子水）中，加热煮沸至完全溶解，分装，1115℃高压灭菌20min。倒板前请摇匀。
保存与运输方法
保存：室温密封阴凉干燥处避光保存，3年有效。
运输：室温运输。
质量控制
接种10-100个霉菌于28℃培养48-96h，需生长良好。
注意事项
1）本品仅用作科研用途，不用于体外诊断。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品
|
|
名称 | 规格 |
|
|
Czapek Dox Agar察氏琼脂（查氏培养基） | 250g |
|
|
Czapek Dox Broth察氏肉汤 | 250g |
|
|
Czapek Dox Agar Modified改良察氏琼脂 |
250g |
| Czapek Dox Broth Modified改良察氏肉汤 |
250g |
|
| Salt Czapek Dox Medium高盐察氏培养基 |
250g |
|
| Czapek Yeast Autolysate Agar (CYA)察氏酵母膏琼脂 |
250g |
YPDA Agar Medium YPDA琼脂培养基
YPD Medium（YEPD Medium）；YPDA Medium；YNB；Minimal SD Base；Dropout (DO) Supplements 省却成分添加剂；
产品信息
| 产品名称 | 规格 |
|
|
| YPDA Agar Medium YPDA琼脂培养基 | 350g | ||
| YPDA Medium YPDA液体培养基 | 250g |
产品描述
YPDA Medium，英文全名Yeast Extract Peptone Dextrose Adenine Medium，是在YPD Medium（酵母浸出粉胨葡萄糖培养基）的配方基础上加入腺嘌呤硫酸盐，能够抑制ade1和ade2突变株的回复突变。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
- 称量50g YPD培养基到1L去离子水，搅拌混匀使其溶解。
- 重要：测试培养基pH是否为6.5，若有必要用弱酸或弱碱调整pH。
- 121℃高压灭菌15min。
- 柔光下室温保存高压灭菌后的培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品
| 名称 | 规格 | |
| YPD Agar Medium YPD琼脂培养基 | 350g | |
| YPD Medium YPD液体培养基 | 250g | |
| YPDA Agar Medium YPDA琼脂培养基 | 350g | |
| YPDA Medium YPDA液体培养基 | 250g | |
| YNB Without Amino Acids酵母氮源基础（无氨基酸） | 100g | |
| YNB Without Amino Acids酵母氮源基础（无氨基酸） | 500g | |
| Minimal SD Agar Base SD琼脂基础培养基 | 467g | |
| Minimal SD Base SD基础培养基 | 267g | |
| Minimal SD Agar Base Gal/Raf SD琼脂基础培养基（含半乳糖/棉子糖） | 270g | |
| Minimal SD Base Gal/Raf SD基础培养基（含半乳糖/棉子糖） | 185g |
SD/-His/-Leu Broth 酵母缺陷型培养基（肉汤）
描述
SD/-His/-Leu Broth
产品标签
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | 价格（元） |
| SD/-His/-Leu Broth 酵母缺陷型培养基（肉汤） | MS6381-5L | 5L | 958 |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-His/-Leu Broth就是缺乏组氨酸（His）和亮氨酸（Leu）两种成分的酵母缺陷型液体培养基，主要用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 称取28g粉末加入1L去离子水，搅拌混匀使其溶解。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Trp/-Ura with Agar 酵母缺陷型培养基（琼脂） Dropout (DO) Supplements氨基酸缺失混合物
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Trp/-Ura with Agar就是缺乏色氨酸（Trp）和尿嘧啶（Ura）两种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Trp/-Ura Broth 酵母缺陷型培养基（肉汤）Dropout (DO) Supplements氨基酸缺失混合物
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Trp/-Ura Broth就是缺乏尿嘧啶（Ura）和色氨酸（Trp）两种成分的酵母缺陷型液体培养基，主要用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 称取28g粉末加入1L去离子水，搅拌混匀使其溶解。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Met/-Trp with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-Met/-Trp with Agar 酵母缺陷型培养基（琼脂） | MS6388-5L | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Met/-Trp with Agar就是缺乏甲硫氨酸（Met）和色氨酸（Trp）两种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Leu/-Trp with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-Leu/-Trp with Agar 酵母缺陷型培养基（琼脂） | MS6386-5L | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Leu/-Trp with Agar就是缺乏亮氨酸（Leu）和色氨酸（Trp）两种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Leu/-Trp Broth 酵母缺陷型培养基（肉汤）Dropout (DO) Supplements氨基酸缺失混合物
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 规格 | ||
| SD/-Leu/-Trp Broth 酵母缺陷型培养基（肉汤） | 5L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Leu/-Trp Broth就是缺乏亮氨酸（Leu）和色氨酸（Trp）两种成分的酵母缺陷型液体培养基，主要用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 称取28g粉末加入1L去离子水，搅拌混匀使其溶解。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-His/-Ura with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-His/-Ura with Agar 酵母缺陷型培养基（琼脂） | MS6384-5L | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-His/-Ura with Agar就是缺乏组氨酸（His）和尿嘧啶（Ura）两种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
YPDA Medium YNB SD/-His/-Ura Broth 酵母缺陷型培养基（肉汤）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-His/-Ura Broth 酵母缺陷型培养基（肉汤） | MS6383-5L | 5L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-His/-Ura Broth就是缺乏组氨酸（His）和尿嘧啶（Ura）两种成分的酵母缺陷型液体培养基，主要用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 称取28g粉末加入1L去离子水，搅拌混匀使其溶解。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
YPD Medium（YEPD Medium） SD/-His/-Leu with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-His/-Leu with Agar 酵母缺陷型培养基（琼脂） | MS6382-5L | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-His/-Leu with Agar就是缺乏组氨酸（His）和亮氨酸（Leu）两种成分的酵母缺陷型固体培养基，常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DO with Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
YPD Medium（YEPD Medium）SD/-Ade with Agar 酵母缺陷型培养基（琼脂）
YPD Medium（YEPD Medium）；YPDA Medium；YNB；Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-Ade with Agar酵母缺陷型培养基（琼脂） | MS6370-5L | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base+ DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Ade with Agar就是缺乏腺嘌呤（Ade）的酵母缺陷型固体培养基，常用于单质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末，全部加入500ml去离子水，搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。冷却至~50℃后再倒板。
4） 室温下使得平板培养基凝固，用封口膜封好后倒置保存在4℃。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 | |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-Ura/-Leu Broth | MS6395-5L | 28 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DOwith Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-Ura/-Leu with Agar | MS6396-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
SD/-Ade Broth 酵母缺陷型培养基（肉汤）Dropout (DO) Supplements氨基酸缺失混合物
YPD Medium（YEPD Medium）；YPDA Medium；YNB；Minimal SD Base；Dropout (DO) Supplements氨基酸缺失混合物；
产品信息
| 产品名称 | 产品编号 | 规格 | |
| SD/-Ade Broth酵母缺陷型培养基（肉汤） | MS6369-5L | 10×0.5 L |
产品描述
酵母缺陷型培养基是由SD Base+ DO Supplement，以添加琼脂和未添加琼脂两种方式混合而成的完全培养基，前者是固体培养基，后者是液体培养基（肉汤），用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base，即Minimal Synthetical Defined Medium，是在无氨基酸酵母氮源基础（YNB）上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement，即氨基酸缺陷混合物（或省却成分添加剂）是缺乏一种或多种特定氨基酸的混合物。
SD/-Ade Broth就是缺乏腺嘌呤（Ade）的酵母缺陷型液体培养基，主要用于单质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
培养基配制
1） 取一包粉末加入0.5L去离子水，搅拌混匀使其溶解。
2） 重要：测试培养基pH是否为5.8，若有必要用弱酸或弱碱调整pH。
3） 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-250G | 65 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| DO Supplement -Ade | MS6315-10G | 1.29 | |
| DO Supplement -Met | MS6330-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| DO Supplement -His/-Ade | MS6327-10G | 1.27 | |
| DO Supplement -Trp/-Ade | MS6328-10G | 1.28 | |
| DO Supplement -Ade/-Leu | MS6329-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| DO Supplement -Trp/-His/-Ade | MS6336-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 |
上接附表1酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| SD + DO Broth酵母缺陷型完全培养基（肉汤） | SD/-His Broth | MS6361-5L | 28 |
| SD/-Leu Broth | MS6363-5L | 28 | |
| SD/-Trp Broth | MS6365-5L | 28 | |
| SD/-Ura Broth | MS6367-5L | 28 | |
| SD/-His/-Leu Broth | MS6381-5L | 28 | |
| SD/-His/-Ura Broth | MS6383-5L | 28 | |
| SD/-Leu/-Trp Broth | MS6385-5L | 28 | |
| SD/-Met/-Trp Broth | MS6387-5L | 28 | |
| SD/-Trp/-Ura Broth | MS6389-5L | 28 | |
| SD/-Trp/-His Broth | MS6391-5L | 28 | |
| SD/-Trp/-Ade Broth | MS6393-5L | 28 | |
| SD/-His/-Leu/-Trp Broth | MS6411-5L | 28 | |
| SD/-His/-Leu/-Ura Broth | MS6413-5L | 28 | |
| SD/-His/-Trp/-Ura Broth | MS6415-5L | 28 | |
| SD/-Leu/-Met/-Trp Broth | MS6417-5L | 28 | |
| SD/-Leu/-Trp/-Ura Broth | MS6419-5L | 28 | |
| SD/-Trp/-His/-Ade Broth | MS6421-5L | 28 | |
| SD/-Ade/-His/-Leu/-Trp Broth | MS6431-5L | 28 | |
| SD/-His/-Leu/-Met/-Trp Broth | MS6433-5L | 28 | |
| SD/-His/-Leu/-Trp/-Ura Broth | MS6435-5L | 28 | |
| SD + DOwith Agar酵母缺陷型完全培养基（琼脂） | SD/-His with Agar | MS6362-5L | —— |
| SD/-Leu with Agar | MS6364-5L | —— | |
| SD/-Trp with Agar | MS6366-5L | —— | |
| SD/-Ura with Agar | MS6368-5L | —— | |
| SD/-His/-Leu with Agar | MS6382-5L | —— | |
| SD/-His/-Ura with Agar | MS6384-5L | —— | |
| SD/-Leu/-Trp with Agar | MS6386-5L | —— | |
| SD/-Met/-Trp with Agar | MS6388-5L | —— | |
| SD/-Trp/-Ura with Agar | MS6390-5L | —— | |
| SD/-Trp/-His with Agar | MS6392-5L | —— | |
| SD/-Trp/-Ade with Agar | MS6394-5L | —— | |
| SD/-His/-Leu/-Trpwith Agar | MS6412-5L | —— | |
| SD/-His/-Leu/-Urawith Agar | MS6414-5L | —— | |
| SD/-His/-Trp/-Urawith Agar | MS6416-5L | —— | |
| SD/-Leu/-Met/-Trpwith Agar | MS6418-5L | —— | |
| SD/-Leu/-Trp/-Urawith Agar | MS6420-5L | —— | |
| SD/-Trp/-His/-Adewith Agar | MS6422-5L | —— | |
| SD/-Ade/-His/-Leu/-Trpwith Agar | MS6432-5L | —— | |
| SD/-His/-Leu/-Met/-Trpwith Agar | MS6434-5L | —— | |
| SD/-His/-Leu/-Trp/-Urawith Agar | MS6436-5L | —— |
Czapek Dox Agar 察氏琼脂（查氏培养基）
描述
Czapek Dox Agar 察氏琼脂（查氏培养基）
产品信息
|
产品名称 |
产品编号 | 规格 | 价格（元） |
| Czapek Dox Agar 察氏琼脂（查氏培养基） | MS6604-250G | 250g |
200 |
产品用途
察氏琼脂（Czapek Dox Agar）是一种半合成的固体培养基，含葡萄糖用作碳源，硝酸钠用作唯一氮源，适用于真菌、酵母和土壤细菌的常规培养。
背景介绍
察氏琼脂（Czapek Dox Agar）是根据Thom&Church研发的配方制备的培养基，具限定性化学成分。含硝酸氮用作唯一氮源的一种半合成培养基。广泛用作真菌常规培养的培养基之一。只有非苛养的土壤细菌才能生长。察氏琼脂能用于白色念珠菌的厚垣孢子产生试验。因含不同盐成分具良好的缓冲能力。pH值略高于中性。察氏琼脂支持几乎所有腐生曲霉（具菌丝体和分生孢子形成特征）的大量生长。
根据Raper&Fenell (1965)的报道，添加1%玉米浆有助于绝大多数曲霉属的生长和孢子产生。按照5g/L加入酵母粉以及pH=4.0推荐用于分离土壤真菌。加入30mg/L链霉菌素和2mg/L金霉素能抑制可能存在的细菌菌丛生长。
产品成分（g/L）
| 磷酸氢二钾（Dipotassium phosphate）：1.0g | 硝酸钠（Sodium Nitrate）：3.0g |
| 氯化钾（Potassium Chloride）：0.5g | 七水硫酸镁（Magnesium Sulfate）：0.5g |
| 七水硫酸亚铁（Ferrous Sulfate）：0.01g | 蔗糖（Sucrose）：30.0g |
| 琼脂（Agar）：15.0g | pH（25℃）：7.3±0.2 |
产品特征
1）级别：微生物培养用(For microbiology)
2）粉末外观：白色粉末，均匀无杂质
3）溶液（胶体）：白色，有不溶物
4）凝胶强度：450-650 g/cm2
5）水分：≤5%
产品用法
称取本品50.0g，加入1L蒸馏水（或去离子水）中，加热煮沸至完全溶解，分装，121℃高压灭菌20min。倒板前请摇匀。
保存与运输方法
保存：室温密封阴凉干燥处避光保存，3年有效。
运输：室温运输。
微生物灵敏度实验
根据产品用法配制的培养基，接种如下质控菌株，置于25℃培养箱、培养3~5天。
| 质控菌株 | 生长特征 |
| 黑曲霉CMCC (F) 98003 | 黑色菌落，生长良好 |
注意事项
1）本品仅用作科研用途，不用于体外诊断。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品
| 货号 | 名称 | 规格 |
| MS6604-250G | Czapek Dox Agar察氏琼脂（查氏培养基） | 250g |
| MS6605-250G | Czapek Dox Broth察氏肉汤 | 250g |
| MS6606-250G | Czapek Dox Agar Modified改良察氏琼脂 | 250g |
| MS6607-250G | Czapek Dox Broth Modified改良察氏肉汤 | 250g |
| MS6608-250G | Salt Czapek Dox Medium高盐察氏培养基 | 250g |
| MS6609-250G | Czapek Yeast Autolysate Agar (CYA)察氏酵母膏琼脂 | 250g |
Agar, Gel Strength 900~1200 g/cm2, 琼脂粉 植物研究中常用琼脂粉为凝固基质
琼脂粉一般是从江蓠、紫菜、石花菜中制取的，可用作增稠剂、凝固剂、悬浮剂、乳化剂、稳定剂、保鲜剂、粘合剂和生物培养基。
微生物研究中常用琼脂粉为凝固基质，用来配制各种微生物固体和半固体培养基，比如LB固体培养基，YNB固体培养基，PDA培养基。相较于更传统的琼脂条作为凝固基质，溶解性强，培养基透明度大大提高，利于后续的微生物菌落研究。
植物研究中常用琼脂粉为凝固基质，用来培养各种植物研究材料如水稻，玉米，拟南芥，花卉等培养基的凝固剂，比如MS，B5，N6固体培养基等。
本品凝胶强度900~1200 g/cm2，用作微生物研究和植物组培原料，一般添加量1.5~2.0%。
产品特性
1） CAS NO.：9002-18-0
2） 别名：Agar-agar；Gum agar；琼脂；洋菜粉；寒天粉；
3） 外观： 类白色-淡黄色粉末
4） 干燥失重：≤22%
5） 烧灼残渣：≤5％
6） 水不溶物：≤1％
7） 砷(As) ：≤0.0001％
8） 重金属（以pb计）：≤0.004％
9） 凝胶强度：900~1200g/cm2
10）溶解性：易溶于沸水、缓溶于热水、不溶于冷水
保存与运输方法
保存：室温干燥保存，5年有效。
运输：室温运输。
注意事项
1） 琼脂粉默认使用pH值为中性，当pH＜4无法凝结。
2） 琼脂粉使用过程遇到不凝固，需要考虑以下因素：①培养基pH值过低，偏酸导致水解和不凝；②所用琼脂量不够，增加用量；倒板前未充分混匀，凝固不均匀；③灭菌温度过高，加热时间过长，pH变化或破坏了琼脂的凝固度；④培养基加热溶解不充分，导致没有凝固。
3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。
订购信息：
| 货号 | 产品名称 | 规格 | |
| MX7403-500G | Agar, Gel Strength 900~1200 g/cm2,琼脂粉 | 500g | |
| MX7403-1KG | Agar, Gel Strength 900~1200 g/cm2,琼脂粉 | 2×500g | |
| MX7403-5KG | Agar, Gel Strength 900~1200 g/cm2,琼脂粉 | 10×500g |
相关产品：
| 货号 | 产品名称 | 规格 | |
| MX7402-100G | Phytagel, Plant Tissue Culture植物凝胶（植物组培级） | 100g | |
| MX7402-500G | Phytagel, Plant Tissue Culture植物凝胶（植物组培级） | 500g | |
| MX7401-500G | Agar, Gel Strength＞1200 g/cm2, Plant Tissue Culture琼脂粉（植物组培级） | 500g | |
| MX7401-1KG | Agar, Gel Strength＞1200 g/cm2, Plant Tissue Culture琼脂粉（植物组培级） | 2×500g | |
| MX7401-5KG | Agar, Gel Strength＞1200 g/cm2, Plant Tissue Culture琼脂粉（植物组培级） | 10×500g | |
| MX7401-25KG | Agar, Gel Strength＞1200 g/cm2, Plant Tissue Culture琼脂粉（植物组培级） | 25kg |