产品变色范围：
无氧状态（O2＜0.1%）：浅紫色到粉红色；
有氧状态（O2＞0.5%）：淡蓝色到深蓝色。
储存方法：本品需要在无氧条件下冷藏保存（2~8℃）。
包装10片厌氧指示剂使用方法
厌氧产气袋C-1，10包/袋,每包用于2.5L
微需氧产气袋C-2，10包/袋，每包用于2.5L
CO2产气袋 C-3，10包/袋, 每包用于2.5L
氧气指示剂C-22，10包/袋, 1包/次
厌氧产气袋C-11，10包 每包用于350ml
分类目录归档：金畔生物
Recombinant Human OSM, 209a.a.
产品编号
JJJP5-10μg
Recombinant Human OSM, 209a.a.
10μg
1671
JJJP5-100μg
Recombinant Human OSM, 209a.a.
100μg
11049
JJJP5-500μg
Recombinant Human OSM, 209a.a.
500μg
22520
JJJP5-1mg
Recombinant Human OSM, 209a.a.
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
5008
P13725
E. coli
209aa
23.7kDa
－
About this Antibody
Name
Recombinant Human OSM, 209a.a. (Recombinant Human Oncostatin-M, 209a.a.; rHuOSM, 209a.a.)；重组人肿瘤抑制素M，209a.a.
Synonyms
MGC20461; oncostatin M; oncostatin-M
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuOSM, 209a.a. as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant human OSM (209a.a.) has approximately 43% and 42% amino acid sequence identity with rat and murine OSM.
Amino Acid Sequence
AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRR
包装清单:
产品编号
产品名称
包装
JJJP5-10μg
Recombinant Human OSM, 209a.a.
10μg
JJJP5-100μg
Recombinant Human OSM, 209a.a.
100μg
JJJP5-500μg
Recombinant Human OSM, 209a.a.
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human OSM, 195a.a.
产品编号
JJP53-10μg
Recombinant Human OSM, 195a.a.
10μg
1671
JJP53-100μg
Recombinant Human OSM, 195a.a.
100μg
11049
JJP53-500μg
Recombinant Human OSM, 195a.a.
500μg
22520
JJP53-1mg
Recombinant Human OSM, 195a.a.
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
5008
P13725
E. coli
195aa
22kDa
－
About this Antibody
Name
Recombinant Human OSM, 195a.a. (Recombinant Human Oncostatin-M, 195a.a.; rHuOSM, 195a.a.)；重组人肿瘤抑制素M，195a.a.
Synonyms
MGC20461; oncostatin M; oncostatin-M
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rHuOSM, 195a.a. as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant human OSM (195a.a.) has approximately 46% and 45% amino acid sequence identity with rat and murine OSM.
Amino Acid Sequence
AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSR
包装清单:
产品编号
产品名称
包装
JJP53-10μg
Recombinant Human OSM, 195a.a.
10μg
JJP53-100μg
Recombinant Human OSM, 195a.a.
100μg
JJP53-500μg
Recombinant Human OSM, 195a.a.
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human OSM
产品编号
JJJP5-10μg
Recombinant Human OSM
10μg
1671
JJJP5-100μg
Recombinant Human OSM
100μg
11049
JJJP5-500μg
Recombinant Human OSM
500μg
22520
JJJP5-1mg
Recombinant Human OSM
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
5008
P13725
E. coli
227aa
25.8kDa
－
About this protein
Name
Recombinant Human OSM (Recombinant Human Oncostatin-M; rHuOSM)；重组人肿瘤抑制素M
Synonyms
MGC20461; oncostatin M; oncostatin-M
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuOSM as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant human OSM has approximately 40% and 39% amino acid sequence identity with rat and murine OSM.
Amino Acid Sequence
AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRRT RPSRKGKRLM TRGQLPR
包装清单:
产品编号
产品名称
包装
JJJP5-10μg
Recombinant Human OSM
10μg
JJJP5-100μg
Recombinant Human OSM
100μg
JJJP5-500μg
Recombinant Human OSM
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human LIF
产品编号
JJP54-5μg
Recombinant Human LIF
5μg
669
JJP54-100μg
Recombinant Human LIF
100μg
5736
JJP54-1mg
Recombinant Human LIF
1mg
18335
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
3976
P15018
E. coli
180aa
19.7kDa
－
About this protein
Name
Recombinant Human LIF (Recombinant Human Leukemia Inhibitory Factor; rHuLIF)；重组人白血病抑制因子
Synonyms
CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); Melanoma-derived LPL inhibitor; MLPLI
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuLIF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.2mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature human LIF (180a.a.) shares 78%, 82%, 91%, 88% and 87%a.a. sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively.
Amino Acid Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
包装清单:
产品编号
产品名称
包装
JJP54-5μg
Recombinant Human LIF
5μg
JJP54-25μg
Recombinant Human LIF
25μg
JJP54-100μg
Recombinant Human LIF
100μg
JJP54-1mg
Recombinant Human LIF
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human sCD40 Ligand/TNFSF5
产品编号
JJJP5-10μg
Recombinant Human sCD40 Ligand/TNFSF5
10μg
669
JJJP5-100μg
Recombinant Human sCD40 Ligand/TNFSF5
100μg
3075
JJJP5-1mg
Recombinant Human sCD40 Ligand/TNFSF5
1mg
9825
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
959
JP20965
E. coli
149aa
16.3kDa
－
About this protein
Name
Recombinant Human sCD40 Ligand/TNFSF5 (Recombinant Human soluble CD40 Ligand; rHusCD40L)；重组人可溶性CD40配体/TNFSF5
Synonyms
CD154; CD40LG; gp39; TNFSF5; TRAP; CD154; CD154 antigen; CD40 antigen ligand; CD40 ligand; CD40-L; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5IMD3; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
Endotoxin
Less than 1EU/μg of rHusCD40L as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
Amino Acid Sequence
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
包装清单:
产品编号
产品名称
包装
JJJP5-10μg
Recombinant Human sCD40 Ligand/TNFSF5
10μg
JJJP5-50μg
Recombinant Human sCD40 Ligand/TNFSF5
50μg
JJJP5-100μg
Recombinant Human sCD40 Ligand/TNFSF5
100μg
JJJP5-1mg
Recombinant Human sCD40 Ligand/TNFSF5
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human Flt-3 Ligand
产品编号
JJJP5-10μg
Recombinant Human Flt-3 Ligand
10μg
1671
JJJP5-100μg
Recombinant Human Flt-3 Ligand
100μg
7987
JJJP5-1mg
Recombinant Human Flt-3 Ligand
1mg
25545
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
2323
P49771
E. coli
155aa
17.6kDa
－
About this protein
Name
Recombinant Human Flt-3 Ligand (Recombinant Human Fms-related Tyrosine Kinase 3 Ligand; rHuFlt3-Ligand)；重组人Fms相关酪氨酸激酶3配体
Synonyms
FLT3LG; FL; Flt3 ligand; FLT3L; Flt3L; fms-related tyrosine kinase 3 ligand; SL cytokine
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
Endotoxin
Less than 1EU/μg of rHuFlt3-Ligand as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Flt3-ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt3. Flt3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt3-ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.
Amino Acid Sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
包装清单:
产品编号
产品名称
包装
JJJP5-2μg
Recombinant Human Flt-3 Ligand
2μg
JJJP5-10μg
Recombinant Human Flt-3 Ligand
10μg
JJJP5-100μg
Recombinant Human Flt-3 Ligand
100μg
JJJP5-1mg
Recombinant Human Flt-3 Ligand
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human BAFF Receptor/TNFRSF13C
产品编号
JJJP50-10μg
Recombinant Human BAFF Receptor/TNFRSF13C
10μg
669
JJJP50-100μg
Recombinant Human BAFF Receptor/TNFRSF13C
100μg
3075
JJJP50-500μg
Recombinant Human BAFF Receptor/TNFRSF13C
500μg
5418
JJJP50-1mg
Recombinant Human BAFF Receptor/TNFRSF13C
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
115650
Q96RJ3
E. coli
76aa
7.8kDa
－
About this protein
Name
Recombinant Human BAFF Receptor/TNFRSF13C (Recombinant Human B Cell Activating Factor Receptor; rHuBAFF-R)；重组人B细胞激活因子受体/TNFSF13C
Synonyms
BR3; CD268; TNFRSF13C
Purity
>95% by reduced SDS-PAGE analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to block BAFF induced mouse splenocyte survival is 1.0-5.0μg/ml in the presence of 1.0μg/ml of rHuBAFF.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH8.0, 500mM NaCl.
Endotoxin
Less than 1EU/μg of rHuBAFF-R as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
B Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation.
Amino Acid Sequence
MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG
包装清单:
产品编号
产品名称
包装
JJJP50-10μg
Recombinant Human BAFF Receptor/TNFRSF13C
10μg
JJJP50-100μg
Recombinant Human BAFF Receptor/TNFRSF13C
100μg
JJJP50-500μg
Recombinant Human BAFF Receptor/TNFRSF13C
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human BAFF/TNFSF13B
产品编号
JP536-5μg
Recombinant Human BAFF/TNFSF13B
5μg
669
JP536-100μg
Recombinant Human BAFF/TNFSF13B
100μg
10659
JP536-500μg
Recombinant Human BAFF/TNFSF13B
500μg
18780
JP536-1mg
Recombinant Human BAFF/TNFSF13B
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
10673
Q9Y275
E. coli
153aa
17.2kDa
－
About this protein
Name
Recombinant Human BAFF/TNFSF13B (Recombinant Human B Cell Activating Factor; rHuBAFF)；重组人B细胞激活因子/TNFSF13B
Synonyms
BLyS; CD257; TALL1; THANK; TNFSF13B; ZTNF4; ApoL related ligand TALL-1; B lymphocyte stimulator; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257; CD257 antigen; Dendritic cell-derived TNF-like molecule; DTL; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2μg/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
Endotoxin
Less than 1EU/μg of rHuBAFF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56%a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.
Amino Acid Sequence
MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
包装清单:
产品编号
产品名称
包装
JP536-5μg
Recombinant Human BAFF/TNFSF13B
5μg
JP536-100μg
Recombinant Human BAFF/TNFSF13B
100μg
JP536-500μg
Recombinant Human BAFF/TNFSF13B
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human TNF-β/TNFSF1
产品编号
JP531-10μg
Recombinant Human TNF-β/TNFSF1
10μg
669
JP531-100μg
Recombinant Human TNF-β/TNFSF1
100μg
6138
JP531-500μg
Recombinant Human TNF-β/TNFSF1
500μg
10830
JP531-1mg
Recombinant Human TNF-β/TNFSF1
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
4049
JP1374
E. coli
171aa
18.7kDa
－
About this protein
Name
Recombinant Human TNF-β/TNFSF1 (Recombinant Human Tumor Necrosis Factor-β/TNFSF1; rHuTNF-β/TNFSF1)；重组人肿瘤坏死因子β/TNFS1
Synonyms
LTA; Lymphotoxin alpha; tnfb; TNF-beta; TNFSF1; TNFSF1B; LT; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); TNFBlymphotoxin-alpha; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5pg/ml, corresponding to a specific activity of >2.0×108IU/mg in the presence of actinomycin D.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20.
Endotoxin
Less than 0.1EU/μg of rHuTNF-β/TNFSF1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNFbeta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1.
Amino Acid Sequence
LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L
包装清单:
产品编号
产品名称
包装
JP531-10μg
Recombinant Human TNF-β/TNFSF1
10μg
JP531-100μg
Recombinant Human TNF-β/TNFSF1
100μg
JP531-500μg
Recombinant Human TNF-β/TNFSF1
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human TNF-α/TNFSF2, Variant
产品编号
JP527-10μg
Recombinant Human TNF-α/TNFSF2, Variant
10μg
669
JP527-100μg
Recombinant Human TNF-α/TNFSF2, Variant
100μg
2420
JP527-500μg
Recombinant Human TNF-α/TNFSF2, Variant
500μg
5418
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7124
JP1375
E. coli
151aa
16.9kDa
－
About this protein
Name
Recombinant Human TNF-α/TNFSF2, Variant (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, Variant; rHuTNF-α/TNFSF2, Variant)；重组人肿瘤坏死因子α/TNFSF2变体
Synonyms
Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01ng/ml, corresponding to a specific activity of >1.0×108IU/mg in the presence of actinomycin D.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
Endotoxin
Less than 1EU/μg of rHu TNF-α/TNFSF2, Variant as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
The clinical use of the potent antitumor activity of TNF-α has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-αmutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-α, which binds to the murine TNF-R55 but not to the murine TNF-R75, exhibits retained antitumor activity and reduced systemic toxicity in mice compared with murine TNF-α, which binds to both murine TNF receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo.
Amino Acid Sequence
MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F
包装清单:
产品编号
产品名称
包装
JP527-10μg
Recombinant Human TNF-α/TNFSF2, Variant
10μg
JP527-100μg
Recombinant Human TNF-α/TNFSF2, Variant
100μg
JP527-500μg
Recombinant Human TNF-α/TNFSF2, Variant
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human TNF-α/TNFSF2, His-tag
产品编号
JP522-10μg
Recombinant Human TNF-α/TNFSF2, His-tag
10μg
248
JP522-50μg
Recombinant Human TNF-α/TNFSF2, His-tag
50μg
548
JP522-100μg
Recombinant Human TNF-α/TNFSF2, His-tag
100μg
908
JP522-1mg
Recombinant Human TNF-α/TNFSF2, His-tag
1mg
4828
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7124
JP1375
E. coli
164aa
18.3kDa
His
About this protein
Name
Recombinant Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag)；重组人肿瘤坏死因子α/TNFSF2，His标签
Synonyms
Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
Endotoxin
Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Amino Acid Sequence
MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
包装清单:
产品编号
产品名称
包装
JP522-10μg
Recombinant Human TNF-α/TNFSF2, His-tag
10μg
JP522-50μg
Recombinant Human TNF-α/TNFSF2, His-tag
50μg
JP522-100μg
Recombinant Human TNF-α/TNFSF2, His-tag
100μg
JP522-1mg
Recombinant Human TNF-α/TNFSF2, His-tag
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human Soluble TNF-α (Trimeric form)
产品编号
JP520-10μg
Recombinant Human Soluble TNF-α (Trimeric form)
10μg
356
JP520-100μg
Recombinant Human Soluble TNF-α (Trimeric form)
100μg
1298
JP520-1mg
Recombinant Human Soluble TNF-α (Trimeric form)
1mg
6898
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7124
JP1375
E. coli
157aa
17.3kDa
No
蛋白信息(About this protein)
名称(Name)
Recombinant Human Soluble TNF-α (Trimeric form)；重组可溶性人肿瘤坏死因子α (三聚体)
别名(Synonyms)
Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
产品简介(Background)
Recombinant Human TNF-α contains soluble cytokines (77-233aa) of the protein. This protein was expressed in E.coli and purified without tag. It is expressed as soluble trimer, which has been validated by Size-exclusion chromatography. Apoptosis induced experiments confirmed that the protein had high biological activity.
TNF-α is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily，and it produced by various immune cells, including T cells, B cells, natural killer cells and macrophages. As a transmembrane protein, TNF-α’s membrane-bound site can be cleaved by the metalloprotease ADAM17/TACE, releasing the soluble cytokine into the extracellular space to form polymers [1-2]. The cellular response to TNF-α is mediated through the receptors TNFR1 and TNFR2. TNFR1 can be activated by both the membrane-bound and soluble trimeric forms of TNF-α, and resulting the downstream apoptosis signaling pathway. While the TNFR2 responds to the membrane-bound form of TNF-α, and promotes T cell activation and proliferation [3]. TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease.
产品用途(Applications)
Sandwich ELISA, Cell Culture, Functional Studies, Mass Spectrometry, HPLC, SDS-PAGE
外观(Physical appearance)
Liquid
活性(Biological activity)
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent Killing/apoptosis of L-929 cells is 0.05ng/ml corresponding to a Specific Activity of 2×107 units/mg.
浓度(Concentration)
1mg/ml
纯度(Purity)
Sterile, >98% as determined by SDS-PAGE
储存液(Storage buffer)
PBS (pH7.4)
本产品纯度高，并经过滤除菌处理。本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图1。本产品经过滤除菌处理，可以直接用于培养细胞的刺激。
图1. Recombinant Human Soluble TNF-α (Trimeric form) (JP520)的SDS-PAGE电泳检测图。本产品经BeyoGel? Plus PAGE预制胶(Hepes, 4-20%, 10孔) (JP010523)电泳，蛋白Marker (M)为BeyoColor?彩色预染蛋白分子量标准(6.5-270kD) (JP010071/JP010072)，后续经BeyoBlue?考马斯亮蓝超快染色液(JP010017F)染色。实际效果会因样品种类、检测仪器等的不同而存在差异，图中数据仅供参考。
本产品可有效激活NF-κB的核转运信号通路，使JP6 (REL-associated protein, Rel-A)转移到细胞核内。稳定表达EGFP-JP6的HeLa细胞(C8001)经终浓度为10ng/ml的本产品刺激后EGFP-JP6进入细胞核，需要注意的是随着时间的延长EGFP-JP6会出核再次回到细胞质中，因此加入本产品后观察EGFP-JP6进入细胞核理想的时间为15-60分钟(图2)。
图2. Recombinant Human Soluble TNF-α (Trimeric form) (JP520)激活NF-κB信号通路诱导核转运的效果图。稳定表达EGFP-JP6的HeLa细胞(C8001)加入或不加入终浓度为10ng/ml的本产品，分别在0、30、60和120分钟用荧光显微镜进行拍照。可见在静息状态(Inactive state)下(Control)，EGFP-JP6均匀分布在细胞质中(第1行)；加入TNF-α刺激后，激活状态(Active state)下EGFP-JP6进入细胞核内(第2行，30分钟)，但是随着时间的延长EGFP-JP6会出核再次回到细胞质中(第2行，120分钟)。实际效果会因实验仪器、检测条件等的不同而存在差异，图中效果仅供参考。
本产品与SM-164 (XIAP抑制剂)联合使用可有效诱导细胞凋亡。100nM SM-164 (XIAP抑制剂) (SJP114)与不同浓度的本产品组成的细胞凋亡诱导剂，诱导BGC-823 (人胃癌细胞)(C6123)细胞凋亡的效果参考图3。
图3. Recombinant Human Soluble TNF-α (Trimeric form) (JP520)诱导细胞凋亡效果图。BGC-823 (人胃癌细胞) (C6123)经100nM SM-164 (XIAP抑制剂) (SJP114)与相应浓度的本产品组成的细胞凋亡诱导剂处理5-6小时，后续采用YO-PRO-1 (凋亡与坏死细胞绿色荧光探针) (C2022)对细胞进行染色。图中可见TNF-α三聚体(Trimer)的本产品比常规的TNF-α单体(monomer)有更强的细胞凋亡诱导效果。实际效果会因实验仪器、检测条件等的不同而存在差异，图中效果仅供参考。
本产品经尺寸排阻层析(Size-exclusion chromatography, SEC)验证为三聚体。人体中TNF-α为26kDa的单次跨膜蛋白，TNF-α被TACE/ADAM17 (TNF-α Converting Enzyme/A Disintegrin and Metalloprotease 17)在Ala76-Val77之间切割，成为游离于细胞膜外可溶形式的TNF-α (77-233aa)，无论是单次跨膜的TNF-α，还是游离于细胞膜外可溶形式的TNF-α，都以同源三聚体的构象行使其生物活性[4]。参考图4，本产品经16/60 Beyodex? 200pg验证为三聚体。
图4. Recombinant Human Soluble TNF-α (Trimeric form) (JP520)经使用16/60 Beyodex? 200pg进行凝胶过滤层析(也称尺寸排阻层析)分析确认为三聚体的参考图。牛血清白蛋白(Bovine Serum Albumin, BSA)分子量约为66kDa，是一种常见的蛋白分子量标准，在使用16/60 Beyodex? 200pg检测中A280nm实际出峰位置为78min (见绿色实线)。如果TNF-α为单体形式(TNF-α Monomer, 17.3kDa)，那么在使用16/60 Beyodex? 200pg检测中A280nm理论出峰位置应为95min (见紫色虚线)。本产品在使用16/60 Beyodex? 200pg检测中A280nm实际出峰位置为86min (见蓝色实线)，符合TNF-α三聚体形式(TNF-α Trimer, 51.9kDa)的出峰位置。实际检测结果可能会因样品和检测条件等的不同而存在差异，结果图仅供参考。
包装清单：
产品编号
产品名称
包装
JP520-10μg
Recombinant Human Soluble TNF-α (Trimeric form)
10μg
JP520-100μg
Recombinant Human Soluble TNF-α (Trimeric form)
100μg
JP520-1mg
Recombinant Human Soluble TNF-α (Trimeric form)
1mg
—
说明书
1份
保存条件：
-20℃保存，一年有效。-80℃保存，可以保存更长时间。
注意事项：
由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后，须分装后-20℃或更低温度冻存，尽量避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human TNF-α/TNFSF2
产品编号
JP518-10μg
Recombinant Human TNF-α/TNFSF2
10μg
669
JP518-100μg
Recombinant Human TNF-α/TNFSF2
100μg
3075
JP518-1mg
Recombinant Human TNF-α/TNFSF2
1mg
9825
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7124
JP1375
E. coli
158aa
17.5kDa
－
About this protein
Name
Recombinant Human TNF-α/TNFSF2 (Recombinant Human Tumor Necrosis Factor-α/TNFSF2; rHuTNF-α/TNFSF2)；重组人肿瘤坏死因子α/TNFSF2
Synonyms
Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, 10mM Nacl, pH7.0.
Endotoxin
Less than 1EU/μg of rHuTNF-α/TNFSF2 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Amino Acid Sequence
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
包装清单:
产品编号
产品名称
包装
JP518-10μg
Recombinant Human TNF-α/TNFSF2
10μg
JP518-50μg
Recombinant Human TNF-α/TNFSF2
50μg
JP518-100μg
Recombinant Human TNF-α/TNFSF2
100μg
JP518-1mg
Recombinant Human TNF-α/TNFSF2
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human M-CSF
产品编号
JP513-10μg
Recombinant Human M-CSF
10μg
1671
JP513-100μg
Recombinant Human M-CSF
100μg
8800
JP513-500μg
Recombinant Human M-CSF
500μg
15520
JP513-1mg
Recombinant Human M-CSF
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
1435
JP0109603
E. coli
158aa
36.8kDa
－
About this protein
Name
Recombinant Human M-CSF (Recombinant Human Macrophage Colony Stimulating Factor; rHuM-CSF)；重组人巨噬细胞集落刺激因子
Synonyms
CSF1; CSF-1; colony stimulating factor 1 (macrophage); CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; M-CSF; MCSFlanimostim; MGC31930
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150a.a. portion that is necessary and sufficient for interaction with the receptor. The first 223a.a. of mature human M-CSF shares 88%, 86%, 81% and 74% sequence identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
Amino Acid Sequence
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS
包装清单:
产品编号
产品名称
包装
JP513-10μg
Recombinant Human M-CSF
10μg
JP513-100μg
Recombinant Human M-CSF
100μg
JP513-500μg
Recombinant Human M-CSF
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human PEDF
产品编号
JJP509-5μg
Recombinant Human PEDF
5μg
669
JJP509-100μg
Recombinant Human PEDF
100μg
7993
JJP509-500μg
Recombinant Human PEDF
500μg
14080
JJP509-1mg
Recombinant Human PEDF
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
5176
JP30955
E. coli
399aa
44.4kDa
－
About this protein
Name
Recombinant Human PEDF (Recombinant Human Pigment Epithelium-derived Factor; rHuPEDF)；重组人色素上皮衍生因子
Synonyms
EPC-1; PEDF; Cell proliferation-inducing gene 35 protein; EPC-1PIG35; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM NaCl.
Endotoxin
Less than 1EU/μg of rHuPEDF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Pigment epithelium-derived factor (PEDF) is encoded by the SERPINF1 gene in humans and found in verebrates. It is a secreted phosphoglycoprotein that belongs to the clade F subfamily, serpin superfamily of proteinase inhibitors. The PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is synthesized as a 418a.a. about 50kDa precursor that contains a 19a.a. signal sequence and a 399a.a. mature region that shows a pyroglutamate at Gln20. Like other serpins, it contains three β-sheets, 810 α-helices, and a C-terminal RCL (reactive center loop). Unlike other serpins with Ser protease inhibiting activity. PEDF has functions of inducing extensive neuronal differentiation in retinoblastoma cells, inhibiting of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. PEDF is researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer.
Amino Acid Sequence
QNPASPPEEG SPDPDSTGAL VEEEDPFFKV PVNKLAAAVS NFGYDLYRVR SSTSPTTNVL LSPLSVATAL SALSLGAEQR TESIIHRALY YDLISSPDIH GTYKELLDTV TAPQKNLKSA SRIVFEKKLR IKSSFVAPLE KSYGTRPRVL TGNPRLDLQE INNWVQAQMK GKLARSTKEI PDEISILLLG VAHFKGQWVT KFDSRKTSLE DFYLDEERTV RVPMMSDPKA VLRYGLDSDL SCKIAQLPLT GSMSIIFFLP LKVTQNLTLI EESLTSEFIH DIDRELKTVQ AVLTVPKLKL SYEGEVTKSL QEMKLQSLFD SPDFSKITGK PIKLTQVEHR AGFEWNEDGA GTTPSPGLQP AHLTFPLDYH LNQPFIFVLR DTDTGALLFI GKILDPRGP
包装清单:
产品编号
产品名称
包装
JJP509-5μg
Recombinant Human PEDF
5μg
JJP509-100μg
Recombinant Human PEDF
100μg
JJP509-500μg
Recombinant Human PEDF
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human TSLP
产品编号
JJP504-10μg
Recombinant Human TSLP
10μg
1671
JJP504-100μg
Recombinant Human TSLP
100μg
10650
JJP504-500μg
Recombinant Human TSLP
500μg
18780
JJP504-1mg
Recombinant Human TSLP
1mg
–
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
85480
Q969D9
E. coli
132aa
15.1kDa
－
About this protein
Name
Recombinant Human TSLP (Recombinant Human Thymic Stromal Lymphopoietin; rHuTSLP)；重组人胸腺基质淋巴细胞生成素
Synonyms
thymic stromal lymphopoietin
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3ng/ml, corresponding to a specific activity of >3.3×106IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM NaCl.
Endotoxin
Less than 1EU/μg of rHuTSLP as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Thymic stromal lymphopoietin (TSLP) is a hemopoietic protein belonging to the cytokine family and is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is mainly expressed in a number of tissues including heart, liver and prostate. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of downstream transcription factors. Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP has the functions of enhancing the maturation of CD11c+ dendritic cells and inducing allergic inflammation by directly activating mast cells. It expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states. But the factors inducing the activation of TSLP release are not clearly defined. Human TSLP shares approximately 43%a.a. sequence identity with mouse TSLP.
Amino Acid Sequence
MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ
包装清单:
产品编号
产品名称
包装
JJP504-10μg
Recombinant Human TSLP
10μg
JJP504-100μg
Recombinant Human TSLP
100μg
JJP504-500μg
Recombinant Human TSLP
500μg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human GM-CSF
产品编号
JJP56-5μg
Recombinant Human GM-CSF
5μg
669
JJP56-100μg
Recombinant Human GM-CSF
100μg
6661
JJP56-1mg
Recombinant Human GM-CSF
1mg
21288
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
1437
JP0104141
E. coli
127aa
14.5kDa
－
About this protein
Name
Recombinant Human GM-CSF (Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor; rHuGM-CSF)；重组人粒细胞-巨噬细胞集落刺激因子
Synonyms
CSF2; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostin; molgramostin; sargramostim; Sargramostim
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuGM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. Human GM-CSF shares 54% sequences identity with mouse GM-CSF, but has no biological effects across species. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy and has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients.
Amino Acid Sequence
APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
包装清单:
产品编号
产品名称
包装
JJP56-5μg
Recombinant Human GM-CSF
5μg
JJP56-20μg
Recombinant Human GM-CSF
20μg
JJP56-100μg
Recombinant Human GM-CSF
100μg
JJP56-1mg
Recombinant Human GM-CSF
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human G-CSF
产品编号
JJP52-2μg
Recombinant Human G-CSF
2μg
669
JJP52-10μg
Recombinant Human G-CSF
10μg
1671
JJP52-100μg
Recombinant Human G-CSF
100μg
7987
JJP52-1mg
Recombinant Human G-CSF
1mg
25545
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
1440
JP0109919
E. coli
174aa
18.7kDa
－
About this protein
Name
Recombinant Human G-CSF (Recombinant Human Granulocyte Colony Stimulation Factor; rHuG-CSF)；重组人粒细胞集落刺激因子
Synonyms
CSF3; C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3OS; filgrastim; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin; Pluripoietin
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.
Physical Appearance
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5% trehalose, pH4.0.
Endotoxin
Less than 1EU/μg of rHuG-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition it may function in some adhesion or recognition events at the cell surface.
In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30a.a. signal peptide and have identical amino acid sequences except for a threea.a. insertion (deletion) at the 35tha.a. residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
Amino Acid Sequence
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
包装清单:
产品编号
产品名称
包装
JJP52-2μg
Recombinant Human G-CSF
2μg
JJP52-10μg
Recombinant Human G-CSF
10μg
JJP52-100μg
Recombinant Human G-CSF
100μg
JJP52-1mg
Recombinant Human G-CSF
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human SCF
产品编号
JJP57-10μg
Recombinant Human SCF
10μg
1671
JJP57-100μg
Recombinant Human SCF
100μg
4913
JJP57-1mg
Recombinant Human SCF
1mg
15717
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
4254
JP20583
E. coli
164aa
18.5kDa
－
About this protein
Name
Recombinant Human SCF (Recombinant Human Stem Cell Factor; rHuSCF)；重组人干细胞生长因子
Synonyms
c-kit Ligand; KITLG; MGF; SLF; DKFZJP66F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KL-1; Mast cell growth factor; MGFSHEP7; SCFStem cell factor; SFc-Kit ligand; steel factor
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuSCF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Stem Cell Factor (SCF) that binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival. Human SCF shares 79%-87%a.a. sequence identity with canine, feline, mouse, and rat SCF. Furthermore, human SCF is weakly active on mouse cells.
Amino Acid Sequence
EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA
包装清单:
产品编号
产品名称
包装
JJP57-2μg
Recombinant Human SCF
2μg
JJP57-10μg
Recombinant Human SCF
10μg
JJP57-100μg
Recombinant Human SCF
100μg
JJP57-1mg
Recombinant Human SCF
1mg
–
说明书
1份
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。