体外研究：

细胞类型（Cell type）：Hepatocytes (from rat liver) in Primary Culture

药物配制（Preparation）：MPA was dissolved in DMSO

实验方法（Assay）：MPA was dissolved in DMSO and added in the appropriate concentrations (0.6, 1.2, 2.4, 5, 10 μg/ml). The amount of DMSO added to the medium was never more than 2 μL/mL, which does not affect hepatocyte proliferation. The in vitro DNA synthesis was detected by [3H] Thymidine Incorporation.

体内研究：

动物模型（Animal Model）：70% partial hepatectomy rat model

药物配制（Preparation）：MPA was dissolved in 0.5% carbossil-methil-cellulose (CMC), 0.4% Tween, and 0.9% alcohol in saline.

注射剂量（Dosages）：Rats were assigned to groups and treated for 4 days as controls or with MPA (15mg/kg).On the 4th day, between 0900 and 1030 hours, the rats received a standard 70% hepatectomy under light ether anesthesia. Food and drink were allowed immediately. Twenty-four hours after the hepatectomies, 185 × 10−4Bq [3H] thymidine was administered to all rats by intraperitoneal injection.

给药途径（Administration）：Oral (p.o.)

体外研究：

细胞类型（Cell type）：PBMCs

药物配制（Preparation）：MPA was dissolved in Ethanol

实验方法（Assay）：PBMCs was placed into each well of a 96-well flat-bottomed microplate.Saline containing concanavalin A was added to each well to a final mitogen concentration of 5.0 µg/ml. Subsequently, an ethanol solution containing MPA was added to give a final drug concentration of 0.001, 0.01, 0.1, 1, 10, 100, 1,000, or 10,000 ng/ml. The same volume of each vehicle solution was added to the control wells (no concanavalin A or MPA treatment). After 4 days of culture, MTT assay was done.