抗真菌剂,抑制农杆菌生长 Plant Preservative Mixture (PPM) 植物组培抗菌剂

植物培养抗菌剂(plant culture preservative),农药(biocide),抗真菌剂,抑制农杆菌生长;

作用机制:

PPM(Plant Preservative Mixture,植物组培抗菌剂)是用于植物细胞培养中的一种强效且广谱的生物杀灭剂,由多种抗菌剂组合而成的混合物,能杀死植物组培培养基和污染组织内的细菌和真菌,作用机制在于能够穿透细菌或真菌细胞壁,抑制柠檬酸循环和电子传递链等中心代谢循环中关键酶的生物活性,以及抑制培养基内单糖和氨基酸往细菌或者真菌细胞的运输。取决于PPM剂量和污染程度,PPM可用作植物培养基内的生物杀灭剂组分。PPM还可用作生物静力化合物起到预防效果。除此之外,PPM能有效抑制植物培养液体或半固体培养中通过空气、水、人传播的微生物污染以及内源性污染,但不会影响体外种子发芽、愈伤组织生长以及根茎再生。

适用植物类型:

PPM有效作用于大多数种子裸露植物(被子植物和裸子植物),但不适合用于蕨类,藓类,藻类和水生植物。【PPM是一种非常有效的抗微生物剂以预防和去除组培污染,然而不能替代实验室的无菌处理试剂,建议使用合适的空气无菌处理系统。】

产品优势【与传统的植物组培抗微生物剂相比】:

1)适当浓度下不会影响大多数植物生长;2)极低浓度即可维持无菌的培养环境;3)可预处理新生种子或清洗组织;4)热稳定性强,高压灭菌非常稳定;5)比传统抗生素价格更低廉,可作为组培培养基内的标准成分,并作为常规使用;6)靶向抑制多种酶活性,植株产生耐药性的几率非常低;

产品特性:

外观:清澈,透明至琥珀色溶液,pH 3.8

保存和稳定性:4°C,3年有效

使用特点:1)PPM可在灭菌前加入培养基或者分液前直接加入灭菌培养基;

         2)PPM可在压力15 psi(1.05 kg/cm2),121°C灭菌20min;

         3)PPM需要灭菌后(post-autoclave)后加入含蛋白质的培养基内,之后将混合好的培养基倒入培养皿;

4)工作浓度的PPM(经培养基稀释)可室温稳定存放高达1个月;

使用浓度参考:

PPM使用指南 Maintenance维持培养 Sterilization杀菌 Agrobacterium农杆菌
作用浓度(v/v) 0.05 – 0.2% 5.0%【见注意事项】 0.05%
作用周期 连续性 4 – 12 h 连续性
注意事项 依照植物组织类型而变化,需做剂量优化。新鲜分离的植物原生质体或愈伤组织建议浓度0.5%。更高浓度会引起毒性。 用在3X MS盐培养基内;

不要和其他无菌技术联合使用;不需要调节pH;处理好的组织可直接放入含0.05% PPM的培养基内;

可加入抗生素cocktail内;

产品订购:

货号 品名 规格              货期      
MS0006-30ML      Plant Preservative Mixture (PPM) 植物组培抗菌剂    30ml   现货
MS0006-100ML Plant Preservative Mixture (PPM) 植物组培抗菌剂 100ml 现货

应用文献:

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