Background Information

Vistech iMatrix Gel is recombinant Laminin-511-E8 fragments, Laminin-511 is well known to bind to the integrin α6β1 which is located on the cell surface.

Vistech iMatrix凝胶是重组Laminin-511- e8片段，Laminin-511已知与位于细胞表面的整合素α6β1结合。

Catalog Number

VM-Gel

Amount

6 × 175 μg/tube: Solution（PBS），Concentration 0.5 mg/mL, total 6 × 350 μL

Storage and Stability

The liquid solution is stable at +2℃-+15℃ until the expiration date printed on the label. Protect from light.

Vistech iMatrix Gel is stable at 4℃ for 2 years from the manufacturing date.

Activity

The dissociation constant of the binding activity with integrin α6β1 is under 10nM

Application

iMatrix Gel is able to use as cell culture substrate for various cell types including ES/iPS cells.

iMatrix Gel Precoat Procedure

1.      Dilute the solution with sterile PBS(-). Coat dishes with 0.5 μg/cm², Common culture plates and recommended dosages are detailed in the table as follow.

Culture vessel	Surface area(cm2)	iMatrix Gel（μL）	Total volume
96 well plate	0.32	0.32	64 μL
24 well plate	2	2	0.4 mL
12 well plate	4.5	4.5	0.9 mL
6 well plate	9.6	9.6	2 mL
35mm dish	8	8	1.6 mL
60mm dish	21	21	4.2 mL
100mm dish	55	55	11 mL
625px2 flask	25	25	5 mL
1875px2 flask	75	75	15mL

 

2.      Incubate for 1 h at 37℃, 3 h at room temperature, or over night at 4℃.

3.      Remove remaining fluid from the coated surface. No rinse is needed.

4.      Immediately plate the cells at desired density.

* Don’t allow the plate to dry.

* Briefly spin down all liquid in the tube before use.

* Avoid repeated freeze thaw cycles.

hPSC Passaging Protocol with iMatrix Gel Adding Medium

1. Remove the culture medium.

2. Wash gently with DPBS. Use more than the volume of the spent culture medium.

3. Add the cell dissociation buffer. Incubate at 37°C for 5-7 min.

4. Transfer the cells to a conical tube. Add the culture medium with more than 3 times the volume of dissociation buffer.

5. Centrifuge at 200 g for 3 min.

6. Remove the supernatant fluid. Resuspend gently the cell pellet with appropriate amount of the culture medium.

7. Count the cell number.

8. Seed the cells to new culture plate.

Cell density (recommended)

2×10⁴ cells/cm²，passage every 4 days

1×10⁴ cells/cm²，passage every 5 days

Culture medium:200 μL/cm²

Additives:

Y-27632: 10 μM final concentration

Vistech iMatrix Gel: 0.25 μg/cm²

Culture vessel	Surface area (cm2)	iMatrix Gel (μL)
96 well plate	0.32	0.16
24 well plate	2	1
12 well plate	4.5	2.3
6 well plate	9.6	4.8
35mm dish	8	4
60mm dish	21	10.5
100mm dish	55	27.5
625px2 flask	25	12.5
1875px2 flask	75	37.5

相关商品