BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.

• Increase viable cell density and culture duration to maximize yield
• Use the same Feed for a wide range of HEK293 cell lines
• Fulfill regulatory requirements
  • Chemically-defined, animal component-free formula
  • Drug Master File (DMF) submitted to US FDA

Two-fold increase in titer compared to other commercially-available media

HEK293-6E cells were cultured in duplicate 125mL Erlenmeyer flasks with a 30 mL working volume. Cells were seeded at 3 x 105 cells/mL in BalanCD HEK 293 medium. PEI-mediated transfection was performed on day 3 to produce a biosimilar antibody. BalanCD HEK293 medium was supplemented with a 5% v/v addition of BalanCD HEK293 Feed on days 1-4 post-transfection.  Protein titer was measured on days 7, 9, and 11 post-transfection. Cultures terminated when viability dropped below 70%, (data not shown). Data shown represents the average of two duplicate cultures.