TDB–FITC-CM-dextran
CAS number: not available
FITC-CM-dextrans are supplied as a yellow powder which is freely soluble in water or electrolyte solutions.
The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining
information on the permeability characteristics of cell membranes and tissues. An anionic fluorescent dextran
with carboxyl groups imparting an overall negative charge to the molecule, valuable for gaining information
on the permeability characteristics of cell membranes and tissues. Mechanistic studies of drug delivery using
FITC-CM-dextran have also been reported.
Spectral data: Excitation is best performed at 493 nm and fluorescence measured at 517 nm.
FITC-CM-dextrans are standard FITC-dextran carrying carboxymethyl-substituents. FITC-CM-dextrans have been developed for special studies on membranes and cells. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB labs produce FITC-CM-dextrans from 4 kDa to 150 kDa. FITC-CM-dextran is supplied as a yellow powder.
Can’t find what you are looking for? We can always produce a customised product for you. Read more here.
Structure
FITC-CM-dextrans are manufactured by reacting selected dextran fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the dextran chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. The degree of substitution of FITC lies between 0.003 – 0.008.
Storage and stability
FITC-CM-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.
Solubility
FITC-CM-dextran dissolves readily in water.
Application
FITC-CM-dextran are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. The free carboxyl group is also useful for coupling other substances to the dextran chain. Read more about applications here.
| Catalog no | Name | MW (Da) | Packsize |
| FCMD4 | FITC-CM-dextran 4 | 4000 | 100mg |
| FCMD4 | FITC-CM-dextran 4 | 4000 | 1g |
| FCMD20 | FITC-CM-dextran 20 | 20000 | 100mg |
| FCMD20 | FITC-CM-dextran 20 | 20000 | 1g |
| FCMD40 | FITC-CM-dextran 40 | 40000 | 100mg |
| FCMD40 | FITC-CM-dextran 40 | 40000 | 1g |
| FCMD70 | FITC-CM-dextran 70 | 70000 | 100mg |
| FCMD70 | FITC-CM-dextran 70 | 70000 | 1g |
| FCMD150 | FITC-CM-dextran 150 | 150000 | 100mg |
| FCMD150 | FITC-CM-dextran 150 | 150000 | 1g |
References
Click to view publications
- Sankaran, J. et al. Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms. npj Biofilms Microbiomes 5, 1–10 (2019).
- Li, B. et al. Functionalized polymer microbubbles as new molecular ultrasound contrast agent to target P-selectin in thrombus. Biomaterials 194, 139–150 (2019).
- Chen, Y., Zhang, M. & Ren, F. A Role of Exopolysaccharide Produced by Streptococcus thermophilus in the Intestinal Inflammation and Mucosal Barrier in Caco-2 Monolayer and Dextran Sulphate Sodium-Induced Experimental Murine Colitis. Molecules 24, 513 (2019).
- Kojima, T. & Takayama, S. Patchy Surfaces Stabilize Dextran–Polyethylene Glycol Aqueous Two-Phase System Liquid Patterns. Langmuir 29, 5508–5514 (2013).
- Srikantha, N. et al. Influence of molecular shape, conformability, net surface charge, and tissue interaction on transscleral macromolecular diffusion. Exp. Eye Res. 102, 85–92 (2012).
- Asgeirsson, D., Venturoli, D., Rippe, B. & Rippe, C. Increased glomerular permeability to negatively charged Ficoll relative to neutral Ficoll in rats. Am. J. Physiol. Renal Physiol. 291, F1083-1089 (2006).
Technical documents
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Download SDS
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Download datafile
Specifications
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FITC-CM-dextran 4
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FITC-CM-dextran 20
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FITC-CM-dextran 70
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FITC-CM-dextran 150
How to order
Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.