TDB–Tetramethylrhodamine Hyaloronic Acid
CAS number: not available
THA-Se is supplied a red solid which is soluble in water. Tetramethyl-rhodamine
hyaluronic acid (THA-Se) has similar applications to those described for fluoresce
in hyaluronic acid (see FHA-Se) but has certain advantages. As mentioned earlier,
the fluorescence of tetramethyl-rhodamine is less dependent on pH than FITC
labels. Also, the longer emission wavelength avoids interference from background
images in experimental environments. Invasive growth into brain tissue employing
TR-HA and 2-photon imaging has been described.
Spectral data: Excitation is best performed at 552 nm and fluorescence measured
at 576 nm.
Tetramethylrhodamine Hyaluronic Acid
Hyaluronic acid (HA) can be obtained from various sources, for example rooster combs or Streptococcus equi. We can supply derivatives of either of these qualities labelled with tetramethylrhodamine (T-HA). All batches are checked for molecular weight, degree of substitution, loss on drying and free TRITC. TdB Labs can produce T-HA with a molecular weight of approximately 1.5 MDa. T-HA is supplied as a pink powder.
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Structure
Hyaluronic acid is a polysaccharide composed of alternating β(1-3) glucuronide and β(1-4) glucosaminide units derived from Streptococcus equi. The hyaluronic acid is labelled with amino-tetramethylrhodamine. The degree of substitution lies between 0.001 and 0.008. The labelling procedures do not lead to depolymerisation of the HA.
Spectral data
T-HA has an excitation maximum at 552 nm and an emission maximum at 576 nm.
Storage and stability
T-HA is stable for more than 6 years when stored dry in well-sealed containers.
Solubility
T-HA dissolves readily in water.
Application
T-HA have been used to e.g. investigate invasive growth into brain tissue. Read more about applications here.
| Catalog no | Name | MW (Da) | Packsize |
| THA-Se | 4T-hyaluronic acid | 5000000 | 100mg |
References
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All publications
- Pusch, A. et al. CD44 and hyaluronan promote invasive growth of B35 neuroblastoma cells into the brain. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research 1803, 261–274 (2010)
- Kaminski, T., Siebrasse, J.-P., Gieselmann, V., Kubitscheck, U., Kappler, J. Imaging and tracking of single hyaluronan molecules diffusing in solution. Glycoconjugate Journal 25(6), 555-560 (2008).
Technical documents
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Download SDS
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Download datafile
How to order
Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.