Megazyme天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒
上海金畔生物科技有限公司
品牌：Megazyme
英文名：Aspartame
货号：K-ASPTM
规格：50 assays (manual) /
分析物意义： 食品中常见的增甜剂有阿斯巴甜、D-甘露醇、D-山梨醇和木糖醇
Megazyme检测试剂盒优点：K-ASPTM-方法新颖/只有试剂盒可用
The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials
Principle:
(pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH
(dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine
(glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate
(L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+
Kit size: 50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition: Novel method
Advantages
- Very cost effective
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Measures aspartame and breakdown products (L-aspartate and aspartame acid)
- Very specific
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
-
件
Megazyme产品所有分类：
生物及食品酶法试剂盒(Assay Kits)
膳食纤维/淀粉(Dietary Fiber/Starch) 单/双糖(Mono/Disaccharides) 多糖(Polysaccharides) 醇类(Alcohols) 有机酸(Organic Acids) 亚硫酸盐/氮(Sulfite/Nitrogen) 活性酶(Enzyme Activity) 试剂混合物(Reagent Mixtures) 其它(Other)
酶(Enzymes)
活性酶(Carbohydrate Active enZYmes )
淀粉酶(Amylases )
阿拉伯树胶酸(Arabinanases)
阿拉伯呋喃(Arabinofuranosidases)
纤维二糖水解(Cellobiohydrolases)
纤维素酶(Cellulases)
酯酶(Esterases)
果糖酶＆果糖苷酶(Fructanases & Fructosidases)
岩藻糖苷酶(Fucosidases)
半乳聚糖酶(Galactanases)
半乳糖苷酶(Galactosidases)
葡聚糖酶(Glucanases)
葡萄糖苷酶(Glucosidases)
葡糖醛酸糖苷(Glucuronidases)
已糖胺酶类(Hexosaminidiases)
裂解酶(Lyases)
甘露聚糖酶(Mannanases)
甘露糖苷酶(Mannosidases)
支链淀粉酶(Pullulanases)
唾液酸酶(Sialidases)
木聚糖酶(Xylanases)
糖苷酶(Xylosidases)
木葡聚糖酶(Xyloglucanases)
多聚半乳糖醛酸(内切)酶(Polygalacturonases)
其它酶(Other Activities)
分析酶(Analytical Enzymes)
脱氢酶(Dehydrogenases)
异构酶(Isomerases)
激酶(Kinases)
磷酸酶(Phosphatases)
蛋白酶(Proteases)
其他活性酶(Other Activities)
糖类活性酶(Glycobiology Enzymes)
酶底物( Chromogenic Substra