Megazyme葡甘露聚糖检测试剂盒

发表于由

Megazyme葡甘露聚糖检测试剂盒
上海金畔生物科技有限公司
品牌：Megazyme

英文名：Glucomannan Assay Kit

货号：K-GLUM

规格：50 Assays per kit

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials

Principle:
(β-mannanase)
(1) Ac-Glucomannan + H2O → Ac-glucomanno-oligomers

(pH 12.5)
(2) Ac-Glucomanno-oligomers + H2O → glucomanno-oligomers
+ acetate

(β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H2O → D-glucose + D-mannose

(hexokinase)
(4) D-glucose + D-mannose + ATP → G-6-P + M-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P → F-6-P → G-6-P

Kit size:                            50 assays 
Method:                            Spectrophotometric at 340 nm
Total assay time:              120 min
Detection limit:                 1-100% of sample weight
Application examples: 
Jelly sweets, cosmetics, food gums and other materials
Method recognition:         Novel method

Advantages

  • Very cost effective
  • Only enzymatic kit available
  • Simple format
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

  •  件

Megazyme产品所有分类：
生物及食品酶法试剂盒(Assay Kits)
膳食纤维/淀粉(Dietary Fiber/Starch) 单/双糖(Mono/Disaccharides) 多糖(Polysaccharides) 醇类(Alcohols) 有机酸(Organic Acids) 亚硫酸盐/氮(Sulfite/Nitrogen) 活性酶(Enzyme Activity) 试剂混合物(Reagent Mixtures) 其它(Other)
酶(Enzymes)
活性酶(Carbohydrate Active enZYmes )
淀粉酶(Amylases )
阿拉伯树胶酸(Arabinanases)
阿拉伯呋喃(Arabinofuranosidases)
纤维二糖水解(Cellobiohydrolases)
纤维素酶(Cellulases)
酯酶(Esterases)
果糖酶＆果糖苷酶(Fructanases & Fructosidases)
岩藻糖苷酶(Fucosidases)
半乳聚糖酶(Galactanases)
半乳糖苷酶(Galactosidases)
葡聚糖酶(Glucanases)
葡萄糖苷酶(Glucosidases)
葡糖醛酸糖苷(Glucuronidases)
已糖胺酶类(Hexosaminidiases)
裂解酶(Lyases)
甘露聚糖酶(Mannanases)
甘露糖苷酶(Mannosidases)
支链淀粉酶(Pullulanases)
唾液酸酶(Sialidases)
木聚糖酶(Xylanases)
糖苷酶(Xylosidases)
木葡聚糖酶(Xyloglucanases)
多聚半乳糖醛酸(内切)酶(Polygalacturonases)
其它酶(Other Activities)
分析酶(Analytical Enzymes)
脱氢酶(Dehydrogenases)
异构酶(Isomerases)
激酶(Kinases)
磷酸酶(Phosphatases)
蛋白酶(Proteases)
其他活性酶(Other Activities)
糖类活性酶(Glycobiology Enzymes)
酶底物( Chromogenic Substra