Megazyme麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒
上海金畔生物科技有限公司
品牌：Megazyme
英文名：Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
货号：K-MBG4
规格：100 assays (manual) / 400 assays (auto-analyser)
alt β-glucanase:
100 assays (manual format) / 400 assays (auto-analyser format)
Lichenase:
100 / 200 assays (manual format) / 330 assays (auto-analyser format)
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase. Mixed linkage
β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g.Trichoderma sp.) but this does not result in an increase in absorbance.
Data calculators are located in the Documentation tab.
DESCRIPTION
Catalase (Aspergillus niger)
EC 1.11.1.16
CAS: 9001-05-2
Synonyms:
catalase; hydrogen-peroxide:hydrogen-peroxide oxidoreductase
Form:
In 3.2 M ammonium sulphate.
Supplied at ~ 18,000 U/mL.
Stability:
> 2 years at 4oC.
Specific activity:
~ 4,960 U/mg protein (using A240 method) at pH 7.0 and 25oC.
Unit definition:
One Unit of catalase activity will decompose 1 micromole of H2O2 per minute at pH 7.0 and 25oC, while the H2O2 concentration falls from 10.3 mM to 9.2 mM. The rate of disappearance of H2O2 is followed by observing the rate of decrease in the absorbance at
240 nm (A240).
Specificity:
Decomposition of hydrogen peroxide into water and oxygen.
Applications:
For use in research.
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件
Megazyme产品所有分类：
生物及食品酶法试剂盒(Assay Kits)
膳食纤维/淀粉(Dietary Fiber/Starch) 单/双糖(Mono/Disaccharides) 多糖(Polysaccharides) 醇类(Alcohols) 有机酸(Organic Acids) 亚硫酸盐/氮(Sulfite/Nitrogen) 活性酶(Enzyme Activity) 试剂混合物(Reagent Mixtures) 其它(Other)
酶(Enzymes)
活性酶(Carbohydrate Active enZYmes )
淀粉酶(Amylases )
阿拉伯树胶酸(Arabinanases)
阿拉伯呋喃(Arabinofuranosidases)
纤维二糖水解(Cellobiohydrolases)
纤维素酶(Cellulases)
酯酶(Esterases)
果糖酶＆果糖苷酶(Fructanases & Fructosidases)
岩藻糖苷酶(Fucosidases)
半乳聚糖酶(Galactanases)
半乳糖苷酶(Galactosidases)
葡聚糖酶(Glucanases)
葡萄糖苷酶(Glucosidases)
葡糖醛酸糖苷(Glucuronidases)
已糖胺酶类(Hexosaminidiases)
裂解酶(Lyases)
甘露聚糖酶(Mannanases)
甘露糖苷酶(Mannosidases)
支链淀粉酶(Pullulanases)
唾液酸酶(Sialidases)
木聚糖酶(Xylanases)
糖苷酶(Xylosidases)
木葡聚糖酶(Xyloglucanases)
多聚半乳糖醛酸(内切)酶(Polygalacturonases)
其它酶(Other Activities)
分析酶(Analytical Enzymes)
脱氢酶(Dehydrogenases)
异构酶(Isomerases)
激酶(Kinases)
磷酸酶(Phosphatases)
蛋白酶(Proteases)
其他活性酶(Other Activities)
糖类活性酶(Glycobiology Enzymes)
酶底物( Chromogenic Substra