产品编号

JJP55-10μg
Recombinant Human IL-36β, 157a.a.
10μg

1671

JJP55-100μg
Recombinant Human IL-36β, 157a.a.
100μg

10650

JJP55-500μg
Recombinant Human IL-36β, 157a.a.
500μg

18780

JJP55-1mg
Recombinant Human IL-36β, 157a.a.
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

27177

Q9NZH7

E. coli

157aa

17.7kDa

－

About this protein

Name

Recombinant Human IL-36β, 157a.a. (Recombinant Human Interleukin-36β, 157a.a.; rHuIL-36β, 157a.a.)；重组人白细胞介素36β，157a.a.

Synonyms

IL36B; IL1F8; IL1H2

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36β at 1μg/ml can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5μg/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-36β, 157a.a. as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36 beta is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin. Furthermore, it can stimulate production of interleukin-6 and interleukin-8 in synovial fibroblasts, articular chondrocytes and mature adipocytes. Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C-terminal 70 amino acid residues have been reported and IL-36β isoform 2 is synthesized as a 157a.a. protein. Specifically, human IL-36β shares low sequence identity with IL-1β, IL-36RA, IL-36α and IL-36γ.

Amino Acid Sequence

MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE

包装清单:

产品编号

产品名称

包装

JJP55-10μg

Recombinant Human IL-36β, 157a.a.

10μg

JJP55-100μg

Recombinant Human IL-36β, 157a.a.

100μg

JJP55-500μg

Recombinant Human IL-36β, 157a.a.

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。