产品编号

JJP506-10μg
Recombinant Human IL-1β
10μg

1671

JJP506-100μg
Recombinant Human IL-1β
100μg

7987

JJP506-1mg
Recombinant Human IL-1β
1mg

25545

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3553

JP1584

E. coli

153aa

17.3kDa

－

About this protein

Name

Recombinant Human IL-1β (Recombinant Human Interleukin-1β; rHuIL-1β)；重组人白细胞介素1β

Synonyms

IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of >1.0×109IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-1β as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL-1β shares 96% amino acid sequence identity with rhesus and 67%-78% with canine, mouse and rat IL-1β.

Amino Acid Sequence

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

包装清单:

产品编号

产品名称

包装

JJP506-2μg

Recombinant Human IL-1β

2μg

JJP506-10μg

Recombinant Human IL-1β

10μg

JJP506-100μg

Recombinant Human IL-1β

100μg

JJP506-1mg

Recombinant Human IL-1β

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。