YPD Medium（YEPD Medium）；YPDA Medium；YNB；Minimal SD Base；DO Supplements 省却成分添加剂；氨基酸缺陷混合物；单缺（SDO）；双缺（DDO）；三缺（TDO）；四缺（QDO）；
产品信息
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产品名称 |
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规格 |
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DO Supplement -Trp/-Leu/-Lys |
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10g |
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DO Supplement -Trp/-Leu/-Lys |
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100g |
产品描述
酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展，这些菌株在缺乏某种特定培养基组分（比如氨基酸，嘌呤或嘧啶）的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长，正因如此，省却成分培养基添加剂（DO medium supplements）能用来筛选转化的酵母培养物。
本品为缺乏色氨酸（Trp）/亮氨酸（Leu）/赖氨酸（Lys）的省却成分培养基添加剂，与Minimal SD base联合使用，配制成三组分缺陷的酵母菌选择性培养基，俗称三缺培养基。用于双质粒酵母转化筛选和报告基因检测。可用于外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统报告基因检测、Y187和AH109酵母交配实验后报告基因分析（His3，Ade2）。
保存与运输方法
保存：2-8°C干燥保存，至少2年稳定。
运输：室温运输。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品
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名称 |
规格 |
价格（元） |
|
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DO Supplement -His/-Leu/-Trp |
10g |
/ |
|
|
DO Supplement -His/-Leu/-Ura |
10g |
/ |
|
|
DO Supplement -His/-Trp/-Ura |
10g |
/ |
|
|
DO Supplement -Leu/-Met/-Trp |
10g |
/ |
|
|
DO Supplement -Leu/-Trp/-Ura |
10g |
/ |
|
|
DO Supplement -Trp/-His/-Ade |
10g |
/ |
|
|
DO Supplement -Trp/-Leu/-Lys |
10g |
/ |
表1酵母培养基配制浓度表
|
培养基类型 |
名称 |
货号 |
终浓度（g/L） |
|
Rich Media 富集培养基 |
YPD Agar Medium |
MS6301-350G |
70 |
|
YPD Medium |
MS6302-250G |
50 |
|
|
YPDA Agar Medium |
MS6303-350G |
70 |
|
|
YPDA Medium |
MS6304-250G |
50 |
|
|
Minimal Media 基本培养基 |
Minimal SD Agar Base |
MS6311-467G |
46.7 |
|
Minimal SD Base |
MS6312-267G |
26.7 |
|
|
Minimal SD Agar Base Gal/Raf |
MS6313-270G |
54 |
|
|
Minimal SD Base Gal/Raf |
MS6314-185G |
37 |
|
|
Single Dropout Supplements (SDO) 单一省却成分添加剂 |
DO Supplement -His |
MS6316-10G |
1.29 |
|
DO Supplement -Leu |
MS6317-10G |
1.29 |
|
|
DO Supplement -Trp |
MS6318-10G |
1.29 |
|
|
DO Supplement -Ura |
MS6319-10G |
1.29 |
|
|
Double Dropout Supplements (DDO) 双省却成分添加剂 |
DO Supplement -His/-Leu |
MS6320-10G |
1.19 |
|
DO Supplement -His/-Ura |
MS6321-10G |
1.27 |
|
|
DO Supplement -Leu/-Trp |
MS6322-10G |
1.19 |
|
|
DO Supplement -Met/-Trp |
MS6323-10G |
1.27 |
|
|
DO Supplement -Trp/-Ura |
MS6324-10G |
1.27 |
|
|
DO Supplement -Trp/-His |
MS6325-10G |
1.27 |
|
|
DO Supplement -Ura/-Leu |
MS6326-10G |
1.27 |
|
|
Triple Dropout Supplements (TDO) 三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp |
MS6331-10G |
1.17 |
|
DO Supplement -His/-Leu/-Ura |
MS6332-10G |
1.17 |
|
|
DO Supplement -His/-Trp/-Ura |
MS6333-10G |
1.25 |
|
|
DO Supplement -Leu/-Met/-Trp |
MS6334-10G |
1.17 |
|
|
DO Supplement -Leu/-Trp/-Ura |
MS6335-10G |
1.17 |
|
|
DO Supplement -Trp/-His/-Ade |
MS6336-10G |
1.25 |
|
|
Quadruple Dropout Supplements (QDO) 四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp |
MS6341-10G |
1.15 |
|
DO Supplement -His/-Leu/-Met/-Trp |
MS6342-10G |
1.15 |
|
|
DO Supplement -His/-Leu/-Trp/-Ura |
MS6343-10G |
1.15 |