描述

Nanosep centrifugal devices are formed out of two components, a filtrate receiver tube and a sample reservoir containing the membrane. These component parts are constructed from low protein binding polypropylene that also offers chemical compatibility for aggressive solvents. Instead of using O-rings or adhesives that could add extractables or bind precious biomolecules the product is constructed using an ultrasonic welding technique.

These centrifugal devices are very simple to use. The sample to be processed is pipetted into the upper reservoir of the device. The device is placed into the appropriate centrifuge (taking care that the rotor is balanced) and spun. Filtrate can be recovered by removing the lower reservoir of the device and using a pipette or pipette tip to remove the liquid. Nanosep centrifugal devices can be used with sample volumes of up to 500 μL, and fit in standard centrifuge rotors that accept 1.5 mL tubes.

Nanosep centrifugal devices with wwPTFE (water wettable PTFE) membranes are available with 0.2 and 0.45 μm pore sizes can be used in rapid microfiltration applications. They are ideal for use in analytical sample preparation or applications that require the use of a universally solvent compatible membrane.

Nanosep centrifugal devices with wwPTFE are certified to be low in UV extractables making them ideal for HPLC or UHPLC sample preparation. They feature low hold-up volumes (<5μL) making these devices ideal for the processing of small volume and expensive samples. Pall also supply the wwPTFE membrane in AcroPrep multi-well filter plates, available in 24, 96 and 384 well configurations that facilitate high-throughput sample preparation. 类型 Centrifugal Filters 用途 Microfiltration, Particulate removal