产品名称

Semaglutide 索马鲁肽

溶剂体积

浓度

体内研究（动物模型）：

动物模型（Animal Model）：C57BL/6J lean male mice at 12 weeks of age

实验方法（Assay）：To study acute inflammation in vivo study design, C57BL/6J lean male mice at 12 weeks of age were given SC doses of semaglutide (60.0 μg/kg) or vehicle control 1 hour prior to receiving an IP dose of lipopolysaccharide (LPS) (0.05 mg/kg, Escherichia coli O55:B5) or vehicle control. Blood samples were collected from the sinus orbital vein at 1 and 4 h after LPS dose.

体外研究：

细胞类型（Cell type）：BV2 cells

实验方法（Assay）： BV2 cells were seeded into 96-well plates (1×10⁴ cells/well) for 12 h, and then treated with different doses (300, 600, 900 or 1,000 nM) of semaglutide for 20 h at 37°C. A blank well with culture medium was used to detect background, and five replicate wells were established per group. CCK-8 reagent (100 µl) was added to evaluate cellular proliferation.

体内研究（动物模型）：

动物模型（Animal Model）：C57BL/6J mice (male, 10±2 weeks old; weight, 25±3 g)

实验方法（Assay）：The animals were randomized into five groups (20 per group), all of which were treated by intraperitoneal (i.p.) injection. In the control group (1% DMSO, i.p.), mice received 20 injections in total – 19 injections every other day for 37 days, and a final injection on the 45th day. For the semaglutide group [25 nM/kg semaglutide i.p.], the administration schedule was the same as that for the control group. In the PTZ group [37 mg/kg PTZ, i.p.], the mice received a total of 16 injections – one injection every other day from the 9th to the 37th day for a total of 15 injections, and a final injection on the 45th day. For the low-dose semaglutide group [37 mg/kg PTZ + 10 nM/kg semaglutide, i.p.], the semaglutide administration schedule was the same as that for the control group, and the PTZ administration schedule was the same as that for the PTZ group, 30 min after each injection of semaglutide. For the high-dose semaglutide group (37 mg/kg PTZ + 25 nM/kg semaglutide, i.p.), the administration schedule was the same as that for the low-dose semaglutide group.