9-Amino-6-chloro-2-methoxyacridine；9-氨基-6-氯-2-甲氧基吖啶；DNA intercalator DNA插入剂；核酸染色剂; Acridine Orange Hydrochloride 吖啶橙盐酸盐；CAS NO：3548-09-2；

产品信息

产品描述

9-氨基-6-氯-2-甲氧基吖啶（9-Amino-6-chloro-2-methoxyacridine, ACMA）是一种细胞通透性的荧光探针，一种DNA嵌入剂，选择性结合poly (dA-dT)序列，随着插入鸟苷荧光寿命逐渐减少。ACMA能用于DNA标记，激发/发射波长为411/475n，可采用大多数紫外光源激发，因而兼容更短和更长波长的染料。ACMA荧光依赖pH，并在形成pH梯度时淬灭，这一特征被用于基于动物和植物的研究中。ACMA也抑制胆碱酯酶活性，Ki为49nM。

产品特性

1） CAS NO：3548-09-2

2） 化学名：6-chloro-2-methoxy-9-acridinamine

3） 同义名：ACMA，NSC 15300

4） 分子式：C₁₄H₁₁ClN₂O

5） 分子量：258.7

6） 外观：固体

7） 纯度：≥98%

8） Ex/Em：411/475nm

9） 溶解性：溶于甲醇

10） 化学结构式：

保存与运输方法

保存：-20°C避光干燥保存，2年有效。

运输：室温运输。

注意事项

1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

染色示例（来自文献，仅作参考）

Ref 1）Jin, R., He, S., Black, K.A. et al. Ion currents through Kir potassium channels are gated by anionic lipids. Nat Commun 13, 490 (2022). https://doi.org/10.1038/s41467-022-28148-4

Assay：Liposomal fluorimetric assay 

Method：The pH sensitive dye 9-amino-chloro-2-methoxyacridine (ACMA) was added to a final concentration of 2 μM from a freshly prepared 200 μM stock in 100% (v/v) ethanol. Fluorescence emission at 483 nm (excitation at 419 nm) was monitored over time with pathlengths of 2 and 10 mm for excitation and emission, respectively.

Ref 2）Furrer EM, Ronchetti MF, Verrey F, Pos KM. Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus. FEBS Lett. 2007 Feb 6;581(3):572-8. doi: 10.1016/j.febslet.2006.12.059. Epub 2007 Jan 17. PMID: 17254570.

Assay：9-Amino-6-chloro-2-methoxyacridine (ACMA) fluorescence assay to detect Na+/H+ antiport activity

Method：Na+/H+ antiporter activity was measured in a thermostated cuvette containing a continuously stirred suspension of 2 ml of 10 mM Tris–HEPES at various pH (6.0–8.0), 140 mM choline chloride, 5 mM MgCl2, 2 μM ACMA and membranes (200 μg of protein). The changes in pH inside the membranes were monitored by following the (de)quenching of ACMA fluorescence (excitation 410 nm; emission 480 nm).

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