描述
IBMX (3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤
产品标签
IBMX PDEs抑制剂；Forskolin (Fsk) 佛司可林；Adenylate cyclase (AC) 腺苷酸环化酶；cAMP 环磷酸腺苷；Adenosine receptor antagonist 腺苷受体拮抗剂；CAS NO：28822-58-4；
产品信息
| 产品名称 | 产品编号 | CAS NO. | 规格 | 价格（元） |
| IBMX (3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤 | MZ2451-50MG | 28822-58-4 | 50mg | 246 |
| IBMX (3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤 | MZ2451-100MG | 28822-58-4 | 100mg | 386 |
| IBMX (3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤 | MZ2451-500MG | 28822-58-4 | 500mg | 1286 |
产品描述
IBMX (3-Isobutyl-1-methylxanthine) ，中文名3-异丁基-1-甲基黄嘌呤，是一种普遍使用的和cAMP和cGMP磷酸二酯酶（PDEs）的非特异性抑制剂（PDE1，PDE2，PDE3，PDE4，PDE5，PDE7和PDE11的IC50分别为19，50，18，13，32，7和50μM）。PDE8A，PDE8B和PDE9对IBMX不敏感。通过抑制PDEs，IBMX增加细胞内cAMP和cGMP水平，激活环化核苷酸调节的蛋白激酶。IBMX能抑制神经内分泌上皮细胞α-肾上腺素受体介导的5-HT释放（IC50=1.3μM）。还是一种非选择性的腺苷受体拮抗剂。能加速小鼠成纤维细胞转化为脂肪细胞。体外还能促进神经祖细胞（NPC）分化。
产品特性
1） CAS NO：28822-58-4
2）化学名：3,7-Dihydro-1-methyl-3-(2-methylpropyl)-1H-purine-2,6-dione 3,7-二氢-1-甲基-3-(2-甲基丙基)- 1H-嘌呤-2,6-二酮；3-Isobutyl-1-methyl-2,6(1H,3H)-purinedione 3-异丁基-1-甲基-2,6(1H,3H)-嘌呤二酮；
3）同义名：3-Isobutyl-1-methylxanthine3-异丁基-1-甲基黄嘌呤；1-Methyl-3-isobutylxanthine1-甲基-3-异丁基黄嘌呤；IMX；MIX；Isobutylmethylxanthine；Methylisobutylxanthine；NSC165960；SC2964；
4）分子式：C10H14N4O2
5）分子量：222.24
6）纯度：≥99%
7）外观：白色或类白色粉末
8）溶解性：溶于乙醇（10mg/ml），DMSO（1M，微热助溶），甲醇（50mg/ml，微热助溶），DMF（5mg/ml），不溶于水。
9）化学结构图：
保存与运输方法
保存：室温干燥保存，或置于-20ºC长期干燥保存，至少2年有效。
运输：室温运输。
使用方法【源自文献，仅作参考】
| 实验目的：3T3-L1小鼠前脂肪细胞诱导分化为成熟的脂肪细胞。
文献1，Chiadak JD et al. Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB. Mediators Inflamm. 2016;2016:1431789. Epub 2016 Nov 2. PMID: 27881903 诱导方法：To induce adipocyte differentiation,3T3-L1 murine preadipocyte cells (day 2) were incubated for 60 h in DMEM supplemented with 10% fetal bovine serum and containing 500 µM IBMX, 0.25 µM dexamethasone, and 10 µg/mL insulin. The cells were then maintained in the culture medium supplemented with insulin only and this media was changed every 2 days (day 5 and day 7) until complete differentiation (monitored by lipid droplet accumulation under the microscope and confirmed by Oil Red Coloration) had occurred (day 9). 文献2，Mudhasani R et al. An Essential Role for Dicer in Adipocyte Differentiation.J Cell Biochem. 2010 Jul 1;110(4):812-6. PMID: 20564208 诱导方法：Mouse embryonic fibroblasts (MEFs)or preadipocytes were then plated at 50–60% confluence and stimulated two days later (considered as day 0 [D0]) with a hormonal cocktail composed of 0.25mM 3-Isobutyl-1-methylxanthine (IBMX), 0.1µM Dexamethasone, Insulin at 10µg/ml and 1µM Rosiglitazone. Starting on day two (D2), the media was replaced every two days with fresh DMEM supplemented with Insulin (10µg /ml) and 1µM Rosiglitazone. Cells were harvested 7–9 days post-induction, fixed in 10% formalin for Oil Red O (ORO) staining. |
| 实验目的：脐带血来源的间充质干细胞（Umbilical cord blood-derived mesenchymal stromal cells, UCB-MSCs）诱导分化为脂肪细胞。
文献3，Rafieemehr H et al. Improving the neuronal differentiation efficiency of umbilical cord blood-derived mesenchymal stem cells cultivated under appropriate conditions. Iran J Basic Med Sci. 2015 Nov;18(11):1100-6. PMID: 26949497 诱导方法：To induce differentiation into adipocytes, cells were plated at 1,000 cells/cm2in 24-well plates in DMEM with 1 μM dexamethasone, 10 μg/ml insulin, 0.5 mM IBMX, and 100 μM indomethacin. After 2 wk of adipogenic stimulation, cells were fixed in 5% PFA for 30 min and incubated with Oil Red-O to stain lipid vacuoles. |
注意事项
1）本品不是临床药物，只能用于科研用途，不能用于诊断或临床用途。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
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