Necrostatin-1 (Necroptosis Inhibitor) 坏死性凋亡抑制剂


描述

Necrostatin-1 (Necroptosis Inhibitor) 坏死性凋亡抑制剂

产品标签

Necrooptosis 坏死性凋亡;Apoptosis 凋亡;Necrostatin(Nec)坏死性凋亡抑制剂;RIP1激酶;RIPK1;CAS NO:4311-88-0;

产品信息

产品名称 产品编号 CAS NO 规格 价格(元)
Necrostatin-1 (Necroptosis Inhibitor) 坏死性凋亡抑制剂 MX3261-10MG 4311-88-0 10mg 650
Necrostatin-1 (Necroptosis Inhibitor) 坏死性凋亡抑制剂 MX3261-100MG 4311-88-0 100mg 2400

产品描述

Necrostatin-1(Nec-1),CAS:4311-88-0,一种特异性且强效的坏死性凋亡(necroptosis)抑制剂,区别于自发凋亡(apoptosis)的一种程序性死亡机制,不会对Fas/TNFR激发的经典自发凋亡级联反应产生任何干扰。Nec-1是一种ATP竞争性的受体相互作用蛋白激酶1(receptor-interacting protein kinase 1, RIPK1, RIP1 kinase)变构抑制剂(EC50=180 nM),该蛋白是参与坏死性凋亡激活途径至关重要的一种上游激酶。小鼠的中风模型中,Nec-1能够降低再灌注引起的脑损伤。

产品特性

1)CAS NO:4311-88-0

2)化学名:5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone

3)同义名:Methylthiohydantoin-DL-tryptophan; Nec-1;

4)分子式:C13H13N3OS

5)分子量:259.33

6)纯度:≥98%

7)溶解性:溶于DMSO(25mg/ml,微热助溶)

8)化学结构式:

保存与运输方法

保存:-20℃干燥保存,2年稳定保存。

运输:室温运输。

注意事项

1)本品在DMSO中的溶解度>10mg/ml,需配制更高浓度或为了更好溶解,请于37℃温热助溶和/或超声波震荡一段时间。储存液可置于-20℃保存数月。

2)本品不是临床药物,只能用于科研用途,不能用于诊断或临床用途。

3)为了您的安全和健康,请穿实验服并戴一次性手套操作。

 

使用方法【源自文献,仅作参考】

文献1,Cui H et al. Necrostatin-1 treatment inhibits osteocyte necroptosis and trabecular deterioration in ovariectomized rats. Sci Rep. 2016 Oct 5;6:33803. PMID: 27703177

体外研究:

细胞类型(Cell type):Murine long bone osteocyte Y4 (MLO-Y4)

实验方法(Assay):The synchronized MLO-Y4, cultured in serum-free medium for 24 h before each experiment, were pretreated for 30 min at 37 °C with DMSO (1%) or Nec-1 (30 mmol/l) and then treated with TNF-α (100 ng/ml) for 24 h.

文献2,Zhou Y et al.Protective effects of necrostatin-1 against concanavalin A-induced acute hepatic injury in mice. Mediators Inflamm. 2013;2013:706156. PMID: 24198446

体内研究:

动物模型(Animal Model):Male C57BL/6 mice (6–8 weeks old, 20 ± 2 g)

实验目的(Objective):To examine the protective effects and mechanisms of Nec-1 in concanavalin A- (ConA-) induced hepatitis in mice.

注射剂量(Dosages):Nec-1 was dissolved in 0.5% DMSO at a concentration of 5mg/mL and was injected intraperitoneally at a dose of 1.8mg/kg body weight. the Nec-1 pretreatment group was injected intraperitoneally with Nec-1 1h before the ConA challenge.

给药途径(Administration):Intraperitoneal (i.p.) injection

文献3,Takemoto K et al.Necrostatin-1 protects against reactive oxygen species (ROS)-induced hepatotoxicity in acetaminophen-induced acute liver failure. FEBS Open Bio. 2014 Sep 6;4:777-87. PMID: 25349782

体内研究:

动物模型(Animal Model):Male C57BL/6 mice, 8–10 weeks old

注射剂量(Dosages):Mice received an intravenous injection of 12.5 mg/kg Nec-1, which was dissolved in DMSO diluted in a warm saline solution. Control mice were intravenously injected with the same volume of DMSO in a warm saline solution.

给药途径(Administration):Intravenous(i.v.) injection

文献4,Dong K et al.Necrostatin-1 protects photoreceptors from cell death and improves functional outcome after experimental retinal detachment. Am J Pathol. 2012 Nov;181(5):1634-41. PMID: 22940440

体内研究:

动物模型(Animal Model):Male Sprague-Dawley rats, weighing 260 to 280 g induced as experimental RD.

实验目的(Objective):Investigate the role of necrostatin-1 on photoreceptor survival and functional protection after experimental retinal detachment (RD) in rats.

注射剂量(Dosages):At RD or 6 hours afterward, 5 μL of 400 μmol/L necrostatin-1, an inactive analogue of necrostatin-1 (necrostatin-1i), or vehicle [DMSO] was administered into the subretinal space of the experimental eyes.

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