MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)


描述

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

 

产品标签

MPP+ iodide;MPTP;帕金森病动物模型;Ferroptosisinhibitor;Necrostatin-1;Ferrostatin-1 (Fer-1);CAS:36913-39-0;

产品信息: 

产品名称

产品编号 CAS NO. 规格 价格(元)

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

MZ6402-50MG 36913-39-0 50mg 922
MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-100MG 36913-39-0 100mg

1592

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide) MZ6402-250MG 36913-39-0 250mg

3522

产品描述

MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)是神经毒素MPTP的活性代谢物,在帕金森病动物模型中选择性破坏多巴胺能神经元。MPP+主要通过抑制线粒体电子传递链复合物I,引起ATP耗竭和氧化应激增高,从而诱导神经毒性。MPP+诱导Necrostatin-1 (Nec-1)和 Ferrostatin-1 (Fer-1)敏感的SH-SY5Y细胞坏死,但MPP+诱导的坏死性细胞死亡与铁坏死是不同的生理过程。

产品特性

1) 化学名:1-Methyl-4-phenylpyridin-1-ium iodide

2) 同义名:N-Methyl-4-Phenylpyridinium Iodide; 1-methyl-4-phenyl-pyridinium, monoiodide; MPP+;N-甲基-4-苯基吡啶鎓碘化物;1-甲基-4-苯基-吡啶鎓碘化物;1-甲基-4-苯基吡啶碘化物;

3) CAS NO:36913-39-0

4) 分子式:C12H12IN

5) 分子量:297.1

6) 纯度:≥98%

7) 外观:固体

8) 溶解性:溶于H2O(100mM)

9) 化学结构式:

保存与运输方法

保存:-20ºC避光干燥保存,至少2年有效。 

运输:冰袋运输。

注意事项

1) 关于化合物溶解性:产品特性内的“≥”表明溶于标示浓度,但饱和溶解度未知。不同批次化合物的溶解度会有差异。

2) 为了让化合物更好的溶解,可通过37℃加热或(和)超声波水浴中震动片刻来处理。若实验所需浓度过大甚至达产品溶解极限,请添加助溶剂助溶或自行调整浓度。

3) 本品仅用作科研用途,不可用于临床或诊疗用途。

4) 为了您的安全和健康,请穿实验服并戴一次性手套操作。

储存液制备

          质量

溶剂体积

浓度

1mg 5mg 10mg
1mM 3.3654 mL 16.8271 mL 33.6542mL
5mM 0.6731mL 3.3654 mL 6.7308mL
10mM 0.3365 mL 1.6827 mL 3.3654 mL

【温馨提示】:请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;MPP+在溶液中不稳定,建议新鲜配制溶液且立即使用。配制好的溶液需避光保存和使用。

使用方法【源自文献,仅作参考】

文献1,Stahl K, Rahmani S, Prydz A, Skauli N, MacAulay N, Mylonakou MN, Torp R, Skare Ø, Berg T, Leergaard TB, Paulsen RE, Ottersen OP, Amiry-Moghaddam M. Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson’s disease. PLoS One. 2018 Mar 22;13(3):e0194896. doi: 10.1371/journal.pone.0194896. PMID: 29566083; PMCID: PMC5864064.

体内研究(动物模型):

动物模型(Animal Model):C57BL/6J WT and Aqp9-/- mice

配制方法(Formulation):MPP+ (7.5 μg dissolved in saline)

实验方法(Assay):C57BL/6J WT (n = 34) and Aqp9-/- mice (n = 29) were deeply anesthetized with zoletil mixture, Rompunand Fentanyl and then subjected tostereotaxic intrastriatal injections of MPP+ (7.5 μg dissolved in saline) or saline. 1 μl MPP+ solution or saline was injected into the striatum 0.6 mm anterior to Bregma, 2.2 mm laterally and 3.2 mm ventrally at 12 μl/hr using a syringe pump. MPP+ was protected against light during the procedure. The injector was left for 5 minutes to allow diffusion before suture.

文献2,Xu J, Gao X, Yang C, Chen L, Chen Z. Resolvin D1 Attenuates Mpp+-Induced Parkinson Disease via Inhibiting Inflammation in PC12 Cells. Med Sci Monit. 2017 Jun 2;23:2684-2691. doi: 10.12659/msm.901995. PMID: 28572562; PMCID: PMC5465971.

体外研究(细胞实验):

细胞类型(Cell types):PC12 cells

实验方法(Assay):The in vitro PD model was established by treatment of PC12 cells with250 μM MPP+ for approximately 24 hours.Different concentrations of RvD1 (50, 100, 200 nM) were administrated two hours prior to MPP+ treatment, afterwards, PC12 cells were used for following experiments.

文献3,Christensen C, Þorsteinsson H, Maier VH, Karlsson KÆ. Multi-parameter Behavioral Phenotyping of the MPP+ Model of Parkinson’s Disease in Zebrafish. Front Behav Neurosci. 2020 Dec 18;14:623924. doi: 10.3389/fnbeh.2020.623924. PMID: 33390914; PMCID: PMC7775599.

体内研究(动物模型):

动物模型(Animal Model):Zebrafish larvae

配制方法(Formulation):Both MPP+ and PBA were dissolved in the system water.

实验方法(Assay):Zebrafish larvae were incubated between 1 and 5 dpf in the presence or absence of 500 μM MPP+ at a total volume of 25 ml system water. The drug solutions were freshly prepared and replaced between 11:00 and 12:00 am at 2, 3, and 4 dpf.Deceased larvae were removed daily, but no differences in mortality were observed between MPP+ exposed larvae and untreated larvae.

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