描述
Forskolin 佛司可林/毛喉素
产品标签
Forskolin (Fsk) 佛司可林；Adenylate cyclase (AC) 腺苷酸环化酶；cAMP 环磷酸腺苷；Neuron Differentiation 神经元分化；CAS NO：66575-29-9；
产品信息
| 产品名称 | 产品编号 | 规格 | CAS NO. | 价格（元） |
| Forskolin (50mM in DMSO) 佛司可林 | MZ2301-50UL | 50μl | 66575-29-9 | 150 |
| Forskolin (50mM in DMSO) 佛司可林 | MZ2301-250UL | 5×50μl | 66575-29-9 | 520 |
| Forskolin (Powder) 佛司可林 | MZ2301-50MG | 50mg | 66575-29-9 | 1480 |
| Forskolin (Powder) 佛司可林 | MZ2301-100MG | 100mg | 66575-29-9 | 2540 |
产品描述
佛司可林（Forskolin，CAS NO.：66575-29-9）是一种来源于印度彩叶植物毛喉鞘蕊花（Coleus forskohlii）的天然生成二萜。通过作用于催化亚基直接激活腺苷酸环化酶（AC），引起细胞内cAMP水平的提高[1-2]。在稳定转染且表达I型腺苷酸环化酶（AC1）的Sf9细胞，Forskolin与膜上的AC1结合（IC50=41nM）并活化其功能（EC50=0.5 μM，评估从ATP生成cAMP的激活实验）[3]。Forskolin以浓度依赖性的方式发挥平滑肌松弛剂和血管扩张剂的功能，同时影响细胞质钙离子水平和收缩成分的钙离子敏感性[4]。Forskolin能够与葡萄糖转运体和某些离子通道相互作用，可用来检测腺苷酸环化酶的表达、调控和G蛋白信号通路[5]。Forskolin用于几种分化方案中以增强神经元分化能力[6-7]。
我司提供两种形式的佛司可林（Forskolin）：
一种是溶于DMSO的50mM储存液形式，对应货号是：MZ2301-50UL（~1mg），MZ2301-250UL（~5mg），直接用培养基稀释到所需工作浓度即可。操作简单方便。
一种是冻干粉形式，对应货号是：MZ2301-50MG，MZ2301-100MG。需要先用DMSO或无水乙醇配制成储存液，再用培养基稀释到所需工作浓度使用。
Forskolin通常用在各种类型的完整细胞或组织培养物中来提高cAMP水平，常用工作浓度为10-50μM。具体的最佳工作浓度请参考文献，或根据自身实验体系，培养细胞和组织类型，通过实验进行摸索和优化。
产品特性
1）CAS NO：66575-29-9
2）化学名：5-(acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-(3R,4aR,5S,6S, 6aS,10S,10aR,10bS)-1H-naphtho[2,1-b]pyran-1-one
3）同义名：Coleonol洋紫苏醇；NSC 375489；NSC 357088；L 75-1362B；HL 362；
4）溶解性：溶于乙醇（~15 mg/ml），DMSO（~30 mg/ml），DMF（~30 mg/ml），几乎不溶于水
5）分子式：C22H34O7
6）分子量：410.50 g/mol
7）纯度：>99%
8）外观：白色结晶性粉末
9）化学结构式：
保存与运输方法
保存：冻干粉-20ºC避光保存，至少3年有效；DMSO储存液-20ºC避光保存至少6个月有效；
运输：冰袋运输。
使用方法【源自文献，仅作参考】
| 文献1，Rodriguez G et al.Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex. J Biol Chem. 2013 Mar 8;288(10):7137-46.PMID: 23341462
体外研究： 细胞类型（Cell type）：Human leukemic cell line MT-2 cells 药物配制（Preparation）：Forskolinwas dissolved in DMSO to make stock solution. 实验方法（Assay）：MT-2 cells were seeded into 96-well plates at 5 × 104 cells per well. Cells were then pretreated for1 h with 1% DMSO (vehicle) or Fsk at 1, 5, 10, 25, 50, and 100 μM concentrations. The cells were stimulated with IL-2 as above and cultured for an additional 20 h at 37 °C. Control cells were treated with 1% DMSO for 20 h. During the final 4 h of incubation, the cells were pulsed with [3H]thymidine at a concentration of 0.5 μCi/200 μl. Cells were harvested onto fiberglass filters and analyzed using liquid scintillation counting.Fsk treatment inhibited the proliferation of MT-2 cells in a dose-dependent manner with an IC50 equal to∼5 μM Fsk. |
| 文献2，Matsumiya W et al. Forskolin modifies retinal vascular development in Mrp4-knockout mice. Invest Ophthalmol Vis Sci. 2012 Dec 7;53(13):8029-35. PMID:23154460
体内研究： 动物模型（Animal Model）：C57BL/6J mice and Mrp4-knockout mice 药物配制（Preparation）：Forskolin was dissolved in dimethyl sulfoxide (DMSO) and then diluted. 注射剂量（Dosages）：Forskolin was dissolved in DMSO and injected intraperitoneally into neonatal mice at postnatal days 4 (P4) (1.0 μg/50 μL (0.3 mg/kg))and 5 (P5)(1.5 μg/50 μL (0.5 mg/kg)). Mice injected with DMSO served as the controls. The treated mice were euthanized at P6, and their retinas were isolated for whole-mount immunohistochemistry (IHC) 给药途径（Administration）：Intraperitoneally (i.p.) Injection |
注意事项
1）本品不是临床药物，只能用于科研用途，不能用于诊断或临床用途。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
参考文献
[1] Barber, R. and Goka, T.J. “Adenylate cyclase activity as a function of forskolin concentration.” J. Cyclic Nucleotide Protein Phosphor. Res. 10: 23-29 (1985).
[2] Laurenza, A., et al. “Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action?” Trends Pharm. Sci. 10: 442-447 (1989).
[3] Robbins JD et al. Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem. 1996 Jul 5;39(14):2745-52.
[4] Abe, A. and Karaki, H.J. “Effect of forskolin on cytosolic Ca++ level and contraction in vascular smooth muscle.” J. Pharmacol. Exp. Ther. 249: 895-900 (1989).
[5] Insel PA et al. Forskolin as a tool for examining adenylyl cyclase expression, regulation, and G protein signaling. Cell Mol Neurobiol. 2003 Jun;23(3):305-14.
[6] Palmer TD et al. The adult rat hippocampus contains primordial neural stem cells.Mol Cell Neurosci. 1997;8(6):389-404.
[7] Son H et al. Pairing of forskolin and KCl increases differentiation of immortalized hippocampal neurons in a CREB Serine 133 phosphorylation-dependent and extracellular-regulated protein kinase-independent manner. Neurosci Lett. 2001 Jul 27;308(1):37-40.
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