品牌：Bioss/博奥森 | 货号：JP-0127R
| 产品编号 | JP-0127R |
| 英文名称 | Bax |
| 中文名称 | Bax抗体 |
| 别 名 | apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4. |
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Specific References (68) | JP-0127R has been referenced in 68 publications.
[IF=7.519] Goreczny,et al.Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1.(2018) Oncogene. 37:1699-1713. IF(ICC) ; Human.
PubMed:29348458
[IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203. IHC-P + WB ; Mouse.
PubMed:29317253
[IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse.
PubMed:30791957
[IF=5.62] Qiu, Shi, et al. “A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53.” Cancer Letters 356.2 (2015): 910-917. IP ; Human.
PubMed:25444901
[IF=5.29] Huang, Chuen-Lin, et al. “Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability.” Molecular Neurobiology (2015): 1-20. Rat.
PubMed:25947082
[IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human.
PubMed:26881030
[IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human.
PubMed:26881030
[IF=4.47] Park, Min-Jung, et al. “New role of irisin in hepatocytes: The protective effect of hepatic steatosis in vitro.” Cellular Signalling (2015). WB ; Mouse.
PubMed:25917316
[IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human.
PubMed:27824118
[IF=3.909] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. IHF ; Rat.
PubMed:30851331
[IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653 WB ; Human.
PubMed:28974930
[IF=3.73] Rani, Neha, et al. “Regulation of Heat Shock Proteins 27 and 70, p-Akt/p-eNOS and MAPKs by Naringin Dampens Myocardial Injury and Dysfunction In Vivo after Ischemia/Reperfusion.” PLOS ONE 8.12 (2013): e82577. WB ; Rat.
PubMed:24324809
[IF=3.73] Zhu, Zhongling, et al. “Regulation of Cell Transformation by Rb-Controlled Redox Homeostasis.” PLOS ONE 9.7 (2014): e102582. WB ; Mouse.
PubMed:25019272
[IF=3.7] Lin, Lai-xiang, et al. “Feasibility of β-Sheet Breaker Peptide-H102 Treatment for Alzheimers Disease Based on β-Amyloid Hypothesis.” PLoS one 9.11 (2014): e112052. IHC-P ; Mouse.
PubMed:25372040
[IF=3.56] He, Xin, et al. “Canonical Wnt signaling pathway contributes to the proliferation and survival in porcine pancreatic stem cells (PSCs).” Cell and tissue research 362.2 (2015): 379-388. WB ; Pig.
PubMed:26085341
[IF=3.457] Chai FN et al. Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.Biomed Pharmacother. 2018 Jul;103:1002-1011. WB ; Mouse.
PubMed:29710498
[IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated JP-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB&IHC-P ; Rat.
PubMed:30813721
[IF=3.33] Chen, Jie, et al. “Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis.” BMC cancer 12.1 (2012): 464. WB ; Human.
PubMed:23050783
[IF=3.33] Tian, Xiao Qiang, et al. “The protective effect of Hyperbaric oxygen and Ginkgo Biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats.” Behavioural Brain Research (2012). WB ; Rat.
PubMed:23266522
[IF=3.265] Zeng Q et al.Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice.(2018) Toxicology. (18)30396-2. WB ; Mouse.
PubMed:30278210
[IF=3.265] Si J et al. Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.(2018) Toxicology. 2018 Oct 12. WB ; zebrafish .
PubMed:30321647
[IF=3.23] Daverey, Amita, and Sandeep K. Agrawal. “Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes.” Neuroscience 333 (2016): 92-103. WB ; Human.
PubMed:27423629
[IF=3.208] Cui H et al. Gamma linolenic acid regulates PHD2 mediated hypoxia and mitochondrial apoptosis in DENinduced hepatocellular carcinoma. Drug Des Devel Ther. 2018 Dec 13;12:4241-4252. WB ; Rat.
PubMed:30587920
[IF=3.156] Andharia,et al.Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.(2018) Pflugers Archiv: European Journal of Physiology. 470:897-907. IHC-P + WB ; Guinea Pig.
PubMed:29399744
[IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493. WB&IHC ; Rat.
PubMed:30505200
[IF=3.13] Zhang, Wen-feng, et al. “Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo.” Phytomedicine (2017). WB ; Human.
PubMed:28887916
[IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. WB ; Chicken.
PubMed:30389602
[IF=3.06] Li, Guiying, et al. “Gremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway.” Journal of Cellular Biochemistry (2013). WB ; Mouse.
PubMed:23553804
[IF=2.86] Liu, Yaping, et al. “Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice.” International Journal of Molecular Sciences 17.1 (2016): 96. WB ; Mouse.
PubMed:26771609
[IF=2.85] Li et al. A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. (2017) PLoS.On. 12:e0175407 WB ; Human.
PubMed:28467514
[IF=2.76] Liu, Qiang, et al. “Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis.” Biomedicine & Pharmacotherapy(2017). WB ; Rat.
PubMed:29198746
[IF=2.75] Ye, Bengui, et al. “Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo.” Journal of Ethnopharmacology (2014). WB ; Human.
PubMed:24583069
[IF=2.69] Lin, Caiyu, et al. “Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo.” Nutrition and Cancer (2014). WB ; Human.
PubMed:24660968
[IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577 WB ;
PubMed:30323629
[IF=2.62] Rahman, Md Mostafizur, et al. “Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism.” Chemico-Biological Interactions (2017). WB ; Rat.
PubMed:28390674
[IF=2.559] Jia C et al. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo(2018) Biochem Biophys Res Commun.505(3):720-725 WB ; Mouse.
PubMed:30292410
[IF=2.491] Şahin S et al. Vitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats.(2018) Epilepsy Res.149 IHC-P ; Rat .
PubMed:30584976
[IF=2.38] She, Yue, et al. “Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats.” Cell Biochemistry and Biophysics (2015): 1-9. IHC-P ; Rat.
PubMed:25578489
[IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. “Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model.” Naunyn-Schmiedeberg’s Archives of Pharmacology (2017): 1-17. IHC-P ; Rat.
PubMed:28070612
[IF=2.27] He, X., et al. “Rapamycin inhibits acrolein‐induced apoptosis by alleviating ROS‐driven mitochondrial dysfunction in male germ cells.” Cell Proliferation (2014). WB ; Mouse.
PubMed:24483236
[IF=2.17] Huang, Guo-fu, et al. “The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration.” Evidence-Based Complementary and Alternative Medicine 2014 (2014). WB ; Rabbit.
PubMed:25763091
[IF=1.894] Chi et al. Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model. (2018) Med.Sci.Monit. 24:3946-3957 WB ; Rat.
PubMed:29888735
[IF=1.66] Wu et al. Resveratrol induces apoptosis in SGC-7901 gastric cancer cells. (2018) Oncol.Lett. 16:2949-2956 WB ; Human.
PubMed:30127883
[IF=1.652] Wang Y et al. Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.(2019)Acta Histochem. Jan;121(1):56-63. WB ; Rat.
PubMed:30392635
[IF=1.63] Aksu, E. H., et al. “Mitigation of paracetamol‐induced reproductive damage by chrysin in male rats via reducing oxidative stress.” Andrologia (2016). IHC-P ; Rat.
PubMed:26914515
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. “Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis.” Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat.
PubMed:27665780
[IF=1.51] Sun, Y‐L., et al. “Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows.” Reproduction in domestic animals 47.4 (2012): 601-608. IHC-P ; Pig.
PubMed:22023076
[IF=1.39] Chen et al. Suppression effect of recombinant adenovirus vector containing hIL-24 on Hep-2 laryngeal carcinoma cells. (2014) Oncol.Let. 7:771-777 WB ; Human.
PubMed:24527085
[IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057 WB ; Human.
PubMed:25120656
[IF=1.39] Chen et al. Suppression effect of recombinant adenovirus vector containing hIL-24 on Hep-2 laryngeal carcinoma cells. (2014) Oncol.Let. 7:771-777 WB ; Human.
PubMed:24527085
[IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057 WB ; Human.
PubMed:25120656
[IF=1.31] Wang, Chunqiang, Wei Ma, and Yuhong Su. “NF-κB Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells.” Biological trace element research (2013): 1-8. WB ; Pig.
PubMed:23575899
[IF=1.243] Li W et al. Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis. (2018) Asian-australas. J. Anim. Sci. Oct 26. WB ; Mouse.
PubMed:30381745
[IF=1.14] Wang, J., et al. “Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway.” Brazilian Journal of Medical and Biological Research (2014): 000-000. WB ; Mouse.
PubMed:25517918
[IF=1.01] Yang, H., et al. “Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro.” Brazilian Journal of Medical and Biological Research 43.11 (2010): 1027-1033. IF(ICC) ; Human.
PubMed:20945038
[IF=.88] Li, Jinglong, et al. “Deficits of learning and memory in Hemojuvelin knockout mice.” Journal of Veterinary Medical Science (2015). WB ; Mouse.
PubMed:26027705
[IF=.44] Sutrisno, Sutrisno, et al. “The effect of genistein on TGF-β signal, dysregulation of apoptosis, cyclooxygenase-2 pathway, and NF-kB pathway in mice peritoneum of endometriosis model.” Middle East Fertility Society Journal (2017). IHC-P ; Mouse.
PubMed:10.1016/j.mefs.2017.05.002
[IF=.2] Sukohar, Asep, and Mohammad Kanedi. “Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells.” Biomedical and Pharmacology Journal 10.2 (2017): 641-649. IF(ICC) ; Human.
PubMed:10.13005/bpj/1151
[IF=0] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human.
PubMed:26538209
[IF=0] Zhang et al. Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice. (2014) Evid.Based.Complement.Alternat.Me. 2013:690808 IHC-P ; Mouse.
PubMed:24454506
[IF=0] Ma et al. A potential adjuvant chemotherapeutics, 18β-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. (2016) Sci.Re. 6:25396 WB ; Human,Mouse.
PubMed:27145860
[IF=0] Han et al. Protective effects of tao-Hong-si-wu decoction on memory impairment and hippocampal damage in animal model of vascular dementia. (2015) Evid.Based.Complement.Alternat.Me. 2015:195835 IHC-P ; Rat.
PubMed:25821478
[IF=0] Xiang M et al. Biyuanling suppresses the toluene-2, 4-diisocyanate induced allergic rhinitis in guinea pigs. Oncotarget. 2017 Dec 8;9(16):12620-12629. WB&IHC-P ; Guinea pigs.
PubMed:29560095
[IF=0] Gao Y et al. Hepatoprotective effects of limb ischemic post-conditioning in hepatic ischemic rat model and liver cancer patients via PI3K/ERK pathways.(2018)Int J Biol Sci. Nov 3;14(14):2037-2050. IHC ; Rat.
PubMed:30585267
[IF=0] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156. WB&IF ; Chicken.
PubMed:30593361
[IF=0] Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer’s disease. J Agric Food Chem. 2019 Aug 27. WB&IHC-P ; Rat.
PubMed:31422666
[IF=] Zou P et al. Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). Int. J. Mol. Sci. 2019, 20, 4470. WB ; Rat.
PubMed:doi:10.3390/ijms20184470
[IF=0] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats. J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB ; Rat.
PubMed:30813721
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| 研究领域 | 细胞生物 信号转导 细胞凋亡 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 21kDa |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Bax:84-175/192 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].
Function: Subunit: Subcellular Location: Tissue Specificity: Similarity: SWISS: Gene ID: Database links: Entrez Gene: 581 Human Entrez Gene: 12028 Mouse Entrez Gene: 24887 Rat Omim: 600040 Human SwissProt: Q07812 Human SwissProt: Q07813 Mouse SwissProt: Q63690 Rat Unigene: 624291 Human Unigene: 19904 Mouse Unigene: 10668 Rat Important Note: 可识别分子量为21KDa的Bax蛋白，此抗体与Bax有较高特异性，且与Bc1-2及Bc1-X蛋白无交叉反应，Bax、Bc1-2和Bc1-X蛋白是凋亡调节蛋白家庭成员。与Bc1-2和Bc1-X相反，Bax蛋白的过量表达加速细胞凋亡。Bax在组织中广泛表达。 |
| 产品图片 |
Sample: Brain(Rat)lysate 30ug;
Liver(Mouse) lysates, 30ug; Primary: Anti-Bax (JP-0127R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 21kD Observed band size : 21kD Sample:
Cerebral cortex (Rat) Lysate at 40 ug Primary: Anti-Bax (JP-0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD Sample:
Hela-UV(Human) Cell Lysate at 30 ug Primary: Anti-Bax (JP-0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 23 kD Sample:
Hela(Human) Cell Lysate at 30 ug Primary: Anti-Bax (JP-0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 23 kD Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (JP-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (JP-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (JP-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(JP-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(JP-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(JP-0127R) 1:800, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (JP-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Overlay histogram showing HL 60 cells stained with JP-0127R (Green line).
The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (JP-0127R,1μg/1×10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (JP- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,JP-0295P,Orange line) (1μg/1×10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. |
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