品牌：Bioss/博奥森 | 货号：JP-0081R
| 产品编号 | JP-0081R |
| 英文名称 | Caspase-3 |
| 中文名称 | 活化半胱胺酸蛋白酶蛋白-3抗体 |
| 别 名 | Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. |
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Specific References (117) | JP-0081R has been referenced in 117 publications.
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[IF=3.52] Chu et al. Newcastle Disease Virus V Protein Inhibits Cell Apoptosis and Promotes Viral Replication by Targeting CacyBP/SIP. (2018) Front.Cell.Infect.Microbiol. 8:304 WB ;
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[IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated JP-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB&IHC-P ; Rat.
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PubMed:not posted yet
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[IF=1.06] Zhang, Xiaolin, and Hao Yu. “Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression (Supplement 2016).” Iranian Journal of Pharmaceutical Research (2016). WB ; Rat.
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[IF=0] Zhou et al. Suppression of E. multilocularis hydatid cysts after ionizing radiation exposure. (2013) PLoS.Negl.Trop.Di. 7:e2518 WB ; Mouse.
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[IF=0] Zhong et al. MicroRNA-200a inhibits epithelial-mesenchymal transition in human hepatocellular carcinoma cell line. (2015) Int.J.Clin.Exp.Patho. 8:9922-31 WB ; Human.
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[IF=0] Pang and Fan Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro. (2016) Int.J.Ophthalmo. 9:497-504 ELISA ; Human.
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[IF=0] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156. WB ; Chicken.
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[IF=.181] Wang S et al. Insulin-like growth factor-1 inhibits the expression of autophagic ID1 in cerebrovascular endothelial cells. Int J Clin Exp Med 2019;12(8):10486-10493. WB ; Human.
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[IF=0] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats. J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB ; Rat.
PubMed:30813721
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| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 Alzheimer’s |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit:1-100/277 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer’s disease. Reacts with Caspase-3 subunit p17 and precursor. Function: Subunit: Subcellular Location: Tissue Specificity: Post-translational modifications: Similarity: SWISS: Gene ID: Database links: Entrez Gene: 836 Human Entrez Gene: 12367 Mouse Entrez Gene: 397244 Pig Entrez Gene: 100008840 Rabbit Entrez Gene: 25402 Rat Omim: 600636 Human SwissProt: P42574 Human SwissProt: P70677 Mouse SwissProt: Q95ND5 Pig SwissProt: Q8MJC3 Rabbit SwissProt: P55213 Rat Unigene: 141125 Human Unigene: 34405 Mouse Unigene: 10562 Rat Important Note: Caspase3广泛分布于各种不同类型的细胞中，是Caspase家族中最重要的凋亡执行者之一，激活的Caspase-3能使许多与细胞结构、细胞周期及DNA修复等相关蛋白或激酶失活，从而使细胞凋亡. |
| 产品图片 |
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Lymph node (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Spleen (Rat) Lysate at 40 ug Lane 7: Lung (Rat) Lysate at 40 ug Lane 8: Lymph node (Rat) Lysate at 40 ug Lane 9: Cerebrum (Rat) Lysate at 40 ug Lane 10: Hela (Human) Cell Lysate at 30 ug Primary: Anti-Caspase-3 (JP-0081R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD Sample:
Lane 1: Raji (Human) Cell Lysate at 30 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: Lung (Mouse) Lysate at 40 ug Lane 4: Lung (Rat) Lysate at 40 ug Primary: Anti-Caspase-3 (JP-0081R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD Sample:
Kidney (Mouse) Lysate at 40 ug Primary: Anti-Caspase-3 (JP-0081R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 17 kD Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti-Caspase-3 (JP-0081R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Sample: Raji Cell lysate;
Primary: Anti-Caspase-3 (JP-0081R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(JP-0295G-HRP) at 1: 5000; Predicted band size: 17/28 kD Observed band size: 26 kD Sample:Liver(Sheep)Lysate at 40 ug
Primary: Anti-Caspase-3(JP-0081R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 30kD Sample:
Lung(Mouse) Lysate at 40 ug Hela(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Caspase-3 (JP-0081R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35/29/19/17 kD Observed band size: 38 kD Protein:Lung(Mouse) lysate at 30ug;
Primary: Anti-caspase-3 (JP-0081R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000; Predicted band size : 17/28 kD Observed band size : 28 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(JP-0081R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rabbit pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(JP-0081R) 1:300, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated (JP-0081R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
The figure annotation:
The blue histogram is unstained cells . The Orange histogram is cells stained with Rabbit IgG/FITC (JP-0295P-FITC). The green histogram is cells stained with Rabbit Anti-Caspase-3/FITC Conjugated antibody (JP-0081R-FITC). Controls Positive control: HepG 2 cells Isotype control: Cell lines treated with Rabbit IgG/FITC (JP-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 1 00μL1 X PBS containing 0.5% BSA. The figure annotation:
The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (JP-0295P-FITC). The green histogram is cells stained with Rabbit Anti-Caspase-3/FITC Conjugated antibody (JP-0081R-FITC). Controls Positive control: A549 cells Isotype control: Cell lines treated with Rabbit IgG/FITC(JP-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 3μg in 1 00 μL 1 X PBS containing 0.5% BSA. Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Caspase-3 antibody (JP-0081R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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