抗体/试剂/诊断抗体原料,核心结合因子α1/成骨特异性转录因子/Cbfα1抗体 | RabbitAnti-RUNX2 antibody

发表于由

品牌：Bioss/博奥森 | 货号：JP-1134R

产品编号 JP-1134R
英文名称 RUNX2
中文名称 核心结合因子α1/成骨特异性转录因子/Cbfα1抗体
别    名 RUNX2_HUMAN; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD.  
Specific References  (10)     |     JP-1134R has been referenced in 10 publications.
[IF=4.095] Gao K et al. Regulation and function of runt-related transcription factors (RUNX1 and RUNX2) in goat granulosa cells.J Steroid Biochem Mol Biol. 2018 Jul;181:98-108.   IHC-P ;  Goat.  
PubMed:29626608

[IF=3.68] Zhang, Ping, et al. “Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones.” Cell and Tissue Research 350.3 (2012): 425-437.  WB ;  Rat.  
PubMed:22237862

[IF=3.414] Lu H et al. Comparative evaluation of book‐type acellular bone scaffold and fibrocartilage scaffold for bone‐tendon healing. J Orthop Res. 2019 Apr 12.   ICF ;  Rabbit.  
PubMed:30977542

[IF=2.784] Chen X et al. Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Send to Int J Mol Med. 2019 Apr 24.   WB ;  Human.  
PubMed:31017269

[IF=2.127] Xu Y et al. Silver nanoparticles promote osteogenic differentiation of human periodontal ligamentfibroblasts by regulating the RhoA-TAZ axis. Cell Biol Int. 2019 May 22.   WB ;  Human.  
PubMed:31115946

[IF=1.892] Li CL et al. Preparation and In Vivo Expression of CS-PEI/pCGRP Complex for Promoting Fracture Healing. International Journal of Polymer Science.2019 9432194.  WB ;  Rat.  
PubMed:doi:10.1155/2019/9432194

[IF=1.872] Li Z et al. Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 2013 Jul;183(1):193-203.   IHC-P ;  Rabbit.  
PubMed:23290592

[IF=1.56] Li, Pengcui, et al. “Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13.” Molecular Medicine Reports.  WB ;  Human.  
PubMed:27356492

[IF=1.26] Sun et al. The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs. (2018) Exp.Ther.Med. 15:3320-3328  IHC-P ;  Guinea Pig.  
PubMed:29545850

[IF=1.2] Hu, Fei, et al. “High expression of periostin is dramatically associated with metastatic potential and poor prognosis of patients with osteosarcoma.” World Journal of Surgical Oncology 12.1 (2014): 287.  IHC-P ;  Human.  
PubMed:25224568

研究领域 干细胞  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 57(hu)/67(mo,ratkDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RUNX2:202-300/521 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008].

Function:
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. [SUBUNIT] Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

Subcellular Location:
Nucleus.

Tissue Specificity:
Specifically expressed in osteoblasts.

Post-translational modifications:
Phosphorylated; probably by MAP kinases (MAPK). Isoform 3 is phosphorylated on Ser340.

DISEASE:
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.

Similarity:
Contains 1 Runt domain.

SWISS:
Q13950

Gene ID:
860

Database links:

Entrez Gene: 860 Human

Entrez Gene: 12393 Mouse

Entrez Gene: 100155806 Pig

Entrez Gene: 367218 Rat

Omim: 600211 Human

SwissProt: Q13950 Human

SwissProt: Q9XSB7 Horse

SwissProt: Q08775 Mouse

SwissProt: Q9Z2J9 Rat

Unigene: 535845 Human

Unigene: 391013 Mouse

Unigene: 391017 Mouse

Unigene: 214214 Rat

Unigene: 83672 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

RUNX2又称：Cbfα1(Core-binding factor, alpha 3 subunit) 是新发现的一类调控间充质干细胞向成骨方向分化的特异性转录因子,参与骨形成,骨骼生长和发育的一类重要细胞,它起源于多能间充质干细胞,是间充质干细胞在体内的各种调控因素的调节下发育而成的。

产品图片
Sample:
Cerebrum (mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Heart (mouse) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti- RUNX2 (JP-1134R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/67 kD
Observed band size: 57 kD

Sample:
HL-60(human)Cell Lysate at 40 ug
Testis (mouse)l Lysate at 40 ug
Primary: Anti- RUNX2 (JP-1134R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/67 kD
Observed band size: 57 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse thyroid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUNX2) Polyclonal Antibody, Unconjugated (JP-1134R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-RUNX2 antibody (JP-1134R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.


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