LBIS® 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE）ELISA试剂盒
LBIS® OVA-IgE Mouse
- 产品特性
- 相关资料
- Q&A
- 参考文献
小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE） ELISA试剂盒
IgE（Immunoglobulin E，免疫球蛋白E）是第五个被发现的免疫球蛋白，由5个结构域（VH、CHε 1～4）构成的两个Hε链和两个L链中组成的IgE，其分子量约190000，电泳实验中向γ1域移动。IgE的代谢半衰期约3天，正常人血清中的IgE浓度非常低，约300 ng/mL。但在寄生虫感染和枯草热时浓度会升高。与过敏性相关的IgE被称作反应素。因接触过敏原导致反应素含量升高，反应素会在Fc域与存在于皮肤、呼吸道、消化脏器中嗜碱性粒细胞和肥大细胞的FcεR1受体结合，引起细胞过敏。在结合过敏原后细胞发生脱粒现象，组胺，五羟色胺，蛋白酶，肝素，趋化因子，前列腺素，白三烯等被投放，经过支气管收缩和黏膜水肿，分泌亢进，从而诱发支气管哮喘，部分荨麻疹，过敏性鼻炎，过敏性反应等I型过敏性反应。
本试剂盒是以OVA（卵清蛋白）作为免疫的抗原，在一个简化的反应系统中通过测定鼠抗OVA- IgE抗体值来进行小鼠免疫系统检测的试剂盒。
◆特点
● 测定时间短（总反应时间：1小时50分钟）。
● 微量样本即可测定。
● 使用无害的防腐剂。
● 全部试剂为溶液即用类型。
● 高测定精度和高重复性。
● 操作简便，无需进行特殊前处理。
◆试剂盒组成
|
组成品 |
状态 |
包装 |
|
OVA包被96孔板（干燥板） |
清洗后使用 |
96 wells（8×12）／1个 |
|
标准溶液（Anti OVA-IgE:1,200 U/mL）（单抗） |
稀释后使用 |
100 μL/1瓶 |
|
缓冲液 |
直接使用 |
60 mL/1瓶 |
|
生物素结合抗小鼠IgE抗体（单抗） |
稀释后使用 |
200 μL/1瓶 |
|
过氧化物酶·抗生素结合物 |
稀释后使用 |
200 μL/1瓶 |
|
显色液（TMB） |
直接使用 |
12 mL/1瓶 |
|
终止液（1M H2SO4）※小心轻放 |
直接使用 |
12 mL/1瓶 |
|
浓缩清洗液（10×） |
稀释后使用 |
100 mL/1瓶 |
|
孔板密封膜 |
3个 |
|
|
产品说明书 |
1本 |
◆样本信息
● 小鼠血清·血浆
● 10 μL/well（稀释样本）
※ 样本需要用试剂盒附带的缓冲液调至标准曲线范围内。
※ 样本必须稀释10倍以上。
◆测定范围
1.88～120 U/mL（标准曲线范围）
（本试剂盒中1 U/mL定义为抗原结合常数（Ka）为2.0×108 M-1的抗体1.3 ng/mL）
◆实验数据
精度测试（组内变异）
|
样本 |
A |
B |
|
1 |
70.7 |
19.1 |
|
2 |
71.0 |
18.7 |
|
3 |
77.1 |
19.6 |
|
4 |
74.3 |
19.7 |
|
5 |
72.9 |
18.9 |
|
Mean |
73.2 |
19.2 |
|
SD |
2.6 |
0.42 |
|
CV（%） |
3.6 |
2.2 |
单位：U/mL
重复性测试（组间变异）
|
测定日/样本 |
C |
D |
E |
|
0天 |
60.0 |
15.0 |
3.75 |
|
1天 |
59.1 |
15.0 |
3.75 |
|
2天 |
58.1 |
14.7 |
3.65 |
|
3天 |
63.6 |
16.0 |
3.48 |
|
Mean |
60.2 |
15.2 |
3.66 |
|
SD |
2.4 |
0.57 |
0.13 |
|
CV（%） |
4.0 |
3.7 |
3.4 |
单位：U/mL
加标回收测试
样本F
|
添加量 |
实测值 |
回收量 |
回收率（%） |
|
0.00 |
6.93 |
— |
— |
|
5.35 |
12.2 |
5.27 |
98.5 |
|
10.7 |
17.8 |
10.9 |
102 |
|
17.1 |
25.1 |
18.2 |
106 |
单位：U/mL，n=3
样本G
|
添加量 |
实测值 |
回收量 |
回收率（%） |
|
0.00 |
40.5 |
— |
— |
|
30.8 |
70.4 |
29.5 |
95.8 |
|
35.9 |
77.1 |
36.6 |
102 |
|
53.9 |
94.9 |
54.4 |
101 |
单位：U/mL，n=3
稀释直线性测试
2个血清样本连续用稀释缓冲液稀释3个梯度测定结果，直线回归值R2=0.9987~0.9999
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| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
| 639-07651 | （AKRIE-030）小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒 LBIS® OVA-IgE Mouse |
96 tests | – | – |
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