Catalog Number:

mLIF Protein

VF-mLIF

Description:

VISTECH® Recombinant Mouse LIF Protein (Vistech mLIF)

Packaging Information:

1.0 ml (10000×)

Working Concentration:

0.1 μL mLIF/ 1 mL culture medium

Background Information:

Mouse Leukemia Inhibitory Factor (mLIF) promotes proliferation and maintains long-term pluripotency of mouse embryonic stem (mES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.

小鼠白血病抑制因子(mLIF)通过抑制小鼠胚胎干细胞(mES)和诱导多能干细胞(iPS)的自发分化，促进细胞增殖并维持其长期多能性。

Applications:

VISTECH® mLIF is supplied in liquid form as 10000× in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability. Vistech mLIF is used as a reagent for the in vitro maintenance of the pluripotential phenotype of mouse ES cells and induced pluripotent stem (iPS) cells. The working concentration is recommended at 0.1 μL mLIF per 1 mL culture medium. So each package of VF-LIF103U can make up 10 L culture medium of mES and iPS.

VISTECH®mLIF以液体形式提供10000x，在1.0 mL磷酸盐缓冲盐水中，以1% w/v牛血清白蛋白BSA作为稳定性载体。Vistech mLIF被用作体外维持小鼠胚胎干细胞和诱导多能干细胞(iPS)多能表型的试剂。推荐工作浓度为每1ml培养基0.1 μL mLIF。因此，每包VF-LIF103U可以组成10 L的mES和iPS培养基。

Storage Conditions:

Shipped at ambient temperature

Storage at 4°C for long term

Don’t freeze-thawing

Stability for at least 18 months from the date of manufacture (in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells)

Stability for at least 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

USE RESTRICTED TO RESEARCH USE ONLY